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心房颤动与白介素-6-174G/C、-572C/G、-597G/A位点基因多态性的关系

郑金国1,高淑萍2,葛利军3,邓新桃1,何永辉1,石桂良1,潘闽4   

  1. 1. 江苏省兴化市人民医院
    2. 宁夏区人民医院心内科
    3. 宁夏自治区人民医院
    4. 南通大学附属医院
  • 收稿日期:2010-06-11 修回日期:2010-08-03 出版日期:2011-01-15 发布日期:2011-01-15
  • 通讯作者: 郑金国

RELATIONSHIP BETWEEN ATRIAL FIBRILLATION AND THE IL-6- 174G/C、-572C/G、-597G/A POLYMORPHISM

  • Received:2010-06-11 Revised:2010-08-03 Published:2011-01-15 Online:2011-01-15

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摘要： 目的：探讨江苏南通地区汉族人群中白介素（IL）-6 -174G/C(rs1800795)、-572C/G(rs1800796)、-597G/A(rs1800797)单核苷酸多态性（SNP）与血清IL -6水平以及房颤发病之间的关系。方法：测定研究对象血清IL-6以及其他临床指标，测量左心房径(LAD)。聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术扩增目的基因片段并酶切。结果：房颤组IL-6和左心房长径与对照组比较差异有统计学意义(P<0.01)；房颤组IL-6 -572C/G基因型分布G/C+G/G明显高于对照组(P<0.05)，且房颤组G等位基因频率明显大于对照组(P<0.01)，IL-6 -174G/C位点C等位基因突变率极低，IL-6-597G/A未见A等位基因突变。结论：IL-6-572C/G位点G等位基因突变导致了机体血清IL-6水平升高，可能与房颤的发病有关。房颤与IL-6多态性的关系尚需进一步扩大样本量作前瞻性研究。

关键词: 心房颤动, 炎症, 白细胞介素 6, 多态性, 单核苷酸, 多态性, 限制性片段长度, 脂类

Abstract: Abstract Objective: To explore the relationships among atrial fibrillation (AF) , Interleukin-6 (IL-6) and single nucleotide polymorphisms (SNP) of the IL-6 -174G/C,-572C/G and -597G/A. Methods: The serum levels of interleukin -6 (IL-6), lipid and other clinical indicators were measured. The left atrial diameter (LAD) were measured by echocardiography (UCG). The frequency distribution of genotypes and alleles of the IL-6 -174G/C,-572G/C and -597G/A in samples of 127 AF patients were amplified by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Results: IL-6 level and LAD of AF group is significantly higher than that of control group(P<0.01). The C allele mutation rate of IL-6 -174G/C is minimal in Han population in Jiangsu Nantong, and IL-6 -597G/A has no A allele mutation in the experiment. IL-6 -572C/G polymorphisms exist in Han population in Jiangsu Nantong, the G/C+G/G genotype distribution and G allele frequencies of IL-6 -572C/G between Atrial fibrillation group and control group have significant differences (P<0.01). Conclusion: IL-6 level and the pathogenesis of AF may be have some relationships. This study shows that among Han population in Nantong IL-6 -572 C/G polymorphic allele G mutation in the AF group is significantly higher than that of the control group (P<0.01), which may be associated with the inflammation and the pathogenesis of AF.

Key words: atrial fibrillation, inflammation, interleukin-6, polymorphism.single nucleotiide, polymorphism.restriction fragment length, lipids, 脂类