天津医药

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丹曲林对缺血再灌注大鼠心肌糖代谢酶活性的影响

王丽艳1,赵晓丽2,郭彦青2,曹颖2,于公元2,康英姿2   

  1. 1. 天津医科大学生物化学教研室
    2. 天津医科大学
  • 收稿日期:2010-08-27 修回日期:2010-11-22 出版日期:2011-07-15 发布日期:2011-07-15
  • 通讯作者: 康英姿

Effect of Dantrolene on the Activities of Enzymes of Glycometabolism in Rats with Myocardial Ischemia-Reperfusion

  • Received:2010-08-27 Revised:2010-11-22 Published:2011-07-15 Online:2011-07-15

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摘要： 目的 研究丹曲林对缺血再灌注损伤的大鼠心肌细胞中糖酵解和异柠檬酸脱氢酶活性的影响。方法 采用健康雄性Vista大鼠（n=16）建立Langendorff离体心脏灌注模型,分为两组，对照组和丹曲林预处理组，对照组全心缺血30分钟，再灌注60分钟；丹曲林预处理组加入5 μM丹曲林后，全心缺血30分钟，再灌注60分钟。用酶联法测定缺血再灌注后心肌糖酵解关键酶—磷酸果糖激酶-1（PFK），丙酮酸激酶(PK)，己糖激酶(HK)，及有氧氧化关键酶—异柠檬酸脱氢酶(IDH)的活性。结果 与对照组相比，丹曲林预处理组冠脉流量增加（P<0.01），磷酸果糖激酶-1、丙酮酸激酶、己糖激酶、异柠檬酸脱氢酶活性均降低（P<0.05）。结论 丹曲林预处理降低了糖代谢中关键酶的活性，减弱了糖的代谢途径，提高了缺血再灌注心脏的功能恢复，对缺血再灌注心脏起到保护作用。

关键词: 丹曲林, 心肌再灌注损伤, 磷酸果糖激酶-1, 丙酮酸激酶, 己糖激酶, 异柠檬酸脱氢酶

Abstract: Objective: To investigate the effect of dantrolene on the activities of enzymes of glycometabolism in rats with myocardial ischemia-reperfusion. Methods: Sixteen healthy male Wistar rats were used in the Langendorff isolated heart perfusion system. The hearts were randomly divided into two groups: control group and dantrolene-treated group, with eight rats in each. The hearts in control group were subjected to 30 min of global ischemia and 60 min of retrograde reperfusion. The hearts in dantrolene-treated group were perfused for 20 min in the working mode, in the presence of 5 μM dantrolene, and then were subjected to 30 min of global ischemia and 60 min of reperfusion. The activities of phosphofructokinase-1(PFK), pyruvate kinase(PK), hexokinase(HK) and isocitrate dehydrogenase (IDH) were determined. Results: The hemodynamic variables had no change except for coronary flow increased (P<0.01). Compared with the control group, the activities of PFK, PK, HK and IDH in dantrolene-treated group were significantly decreased (P<0.05). Conclusion: Dantrolene has a protective effect in myocardial ischemia-reperfusion by reducing the activities of glycolytic enzymes and isocitrate dehydrogenase, slowing the glucose metabolism and thereby enhancing the ability to recover the heart function during ischemia-reperfusion.

Key words: dantrolene, myocardial reperfusion injury, phosphofructokinase-1(PFK), pyruvate kinase(PK), hexokinase(HK), isocitrate dehydrogenase(IDH)

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