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茶多酚治疗N-甲基亚硝基脲诱发膀胱肿瘤的实验研究

梁国庆1,陈春雷1,陈伟杰1,张晓峰2   

  1. 1. 上海中医药大学附属市中医医院泌尿外科
    2. 上海中医药大学附属市中医医院中心实验室
  • 收稿日期:2012-11-08 修回日期:2013-03-23 出版日期:2013-09-15 发布日期:2013-09-15
  • 通讯作者: 陈伟杰

Observation on Epigallocatechin Gallate Treating the Bladder Tumor Induced by N-methyl-N-nitrosourea in Rats

LIANG Guo qing1,CHEN Chun lei1,CHEN Wei jie1,ZHANG Xiao feng2   

  1. 1. Department of Urology,Shanghai Municipal Hospital of Traditional ChineseMedicine
    2. Department of Central Labtoratory,Shanghai Municipal Hospital of Traditional ChineseMedicine
  • Received:2012-11-08 Revised:2013-03-23 Published:2013-09-15 Online:2013-09-15
  • Contact: CHEN Wei jie

摘要:

【摘要】 目的  观察茶多酚对N-甲基亚硝基脲(MNU)诱发膀胱肿瘤的治疗作用。方法  将72只雌性Wistar大鼠随机分为治疗组和对照组,各36只。经膀胱内灌注MNU(2mL/次,2周1次)制作膀胱肿瘤模型,治疗组以茶多酚溶液灌胃,对照组以蒸馏水灌胃,每日1次,每次2mL。实验开始后第3、5、7、9、11和13周,每组各处死6只大鼠,取其膀胱组织标本,显微镜下观察2组膀胱组织病理变化情况并记分。结果  对照组从第5周开始,膀胱黏膜出现明显的不典型增生病变,第11周开始发生膀胱恶性肿瘤;治疗组始终未见膀胱恶性肿瘤。2组病理学变化记分除第3周差异不明显外(P>0.05),其余时间段均是治疗组小于对照组(P<0.05)。对照组大鼠膀胱壁黏膜第3周增厚至5~7层,第7周出现乳头状瘤样变化,第11周出现部分癌变,至第13周细胞异形性明显,排列紊乱,核分裂象明显。治疗组在第5~13周细胞层次轻度增厚至3~5层,细胞排列有序,核浆比例基本正常。结论  茶多酚可有效治疗MNU诱发的膀胱肿瘤。

关键词: 膀胱肿瘤, 茶, 酚类, 甲基亚硝脲, 模型, 动物, 大鼠, Wistar

Abstract:

[Abstract]   Objective   Toinvestigate the therapeutic effect of epigallocatechin gallate (EGCG) on the bladder neo-
plasm induced by N-methyl-N-nitrosourea (MNU) in Wistar rats.Methods   Seventy-two female rats were randomly divided into two groups, treatment group and control group. The rat model of bladder tumor was established by intravesical instillation of MNU (2mL/time, once every two weeks) inside bladder. Rats in treatment group were given EGCG (2mL/time, once/day), and control group were given the same volume of distilled water. Six rats were executed respectively at the3rd, 5th,7th,9th, 11th and13th  week, and the bladder histopathological changes were studied in two groups.Results  The untypical hyper-plasia was observed after5weeks. The bladder neoplasm was observed at the11th week in control group. The bladder neo-plasm was not found in treatment group. There was a significantly lower   pathological score in treatment group than that of control group except for the first two weeks (P<0.05).   Conclusion  EGCG is effective for treatment of the bladder neo-plasm induced by MNU.

Key words: urinary bladder neoplasms, tea, phenols, methylnitrosourea, models, animal, rats, Wistar