天津医药

天津医药 ›› 2014, Vol. 42 ›› Issue (12): 1197-1204.doi: 10.3969/j.issn.0253-9896.2014.12.014

• 临床研究 • 上一篇    下一篇

Hedgehog 信号通路在肾透明细胞癌中的表达及作用

李晓明1,杨春雨1,关有良2,3   

  1. 1. 辽宁医学院
    2. 辽宁医学院附属第一医院泌尿外科
    3.
  • 收稿日期:2014-05-20 修回日期:2014-07-31 出版日期:2014-12-15 发布日期:2014-12-15
  • 通讯作者: 李晓明 E-mail:lixiaoming68327@163.com
  • 基金资助:
    辽宁省自然科学基金项目

Expression and Role of Hedgehog Signaling Pathway in Renal Clear Cell Carcinoma

LI Xiaoming 1,YANG Chunyu 2,GUAN Youliang 3   

  • Received:2014-05-20 Revised:2014-07-31 Published:2014-12-15 Online:2014-12-15
  • Contact: LI Xiaoming E-mail:lixiaoming68327@163.com

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摘要：

【摘要】 目的 研究人肾透明细胞癌（RCCC）中 Hedgehog 信号通路相关因子 Shh、Ptch、Smo 和 Gli1 蛋白的表达， 探讨 Hedgehog 信号转导通路的异常激活与 RCCC 的关系。 方法 应用免疫组织化学方法检测 76 例 RCCC 标本及 12 例癌旁正常肾组织， 应用蛋白免疫印迹技术检测 10 例新鲜癌组织及癌旁正常肾组织中 Shh、Ptch、Smo 和 Gli1 蛋白的表达。 结果 Shh、Ptch、Smo 和 Gli1 蛋白在 RCCC 中的异常表达率（分别为 52.6%、63.2%、44.7%、60.5%）高于正常肾组织(分别为 8.3%、16.7%、8.3%、25.0%)。 且其表达与患者的年龄、性别和肿瘤大小无显著相关， 而与肿瘤临床分期有关， 随肿瘤的恶性程度加重， 表达率增加。 结论 Hedgehog 信号转导通路在 RCCC 中呈现高度激活状态， 此通路的激活可能与 RCCC 的发生、发展密切相关， 有望成为治疗 RCCC 的靶点。

关键词: 肾透明细胞癌, Hedgehog信号通路, 免疫组织化学, 免疫印迹, 蛋白质

Abstract:

[Abstract] Objective To investigate the expression of Shh, Ptch, Smo and Gli1 proteins who belongs to components of Hedgehog signaling pathway, and to explore the relationship between the abnormal activated of Hedgehog signaling path? way with renal clear cell carcinoma (RCCC). Methods Protein expressions were examined by immunohistochemistry in 76 cases of human RCCC and 12 pericarcinomatous tissues. And 10 fresh specimens from cancer tissues and pericarcinoma? tous tissues were used to examine expressions of Shh, Ptch, Smo and Gli1 by Western blotting. Results The expressions of examined proteins were higher in RCCC than that in pericarcinomatous tissue. Shh (52.6% vs 8.3%), Ptch (63.2% vs 16.7%), Smo (44.7% vs 8.3%) and Gli1 (60.5% vs 25.0%). The increased proteins levels were disassociated with age, gender, and tu? morous size in RCCC. But it was significantly correlated with TNM stage of RCCC. The expressions were gradually increased with higher TNM stage of RCCC or more invasive malignancy. Conclusion Hedgehog signaling pathway is highly activated in RCCC. It might be involved in initiation, progression of RCCC, and it might be used as the target for RCCC treatment.

Key words: renal clear cell carcinoma, Hedgehog signaling pathway, immunohistochemistry, Western blot, Western