天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (2): 121-124.doi: 10.11958/j.issn.0253-9896.2015.02.003

• 缺氧与器官损伤 • 上一篇    下一篇

间充质干细胞移植减轻大鼠肺气肿及氧化应激的探讨

金志贤1 ， 毕虹 1 ， 周开华1 ， 杜俊毅 1 ， 陈敏 1 ， 王清 1△， 潘兴华 2△   

  1. 1昆明市第一人民医院呼吸二科 （邮编650011）； 2云南省干细胞工程实验室， 成都军区昆明总医院
  • 收稿日期:2014-09-26 修回日期:2014-11-13 出版日期:2015-02-15 发布日期:2015-02-27
  • 通讯作者: 王清 E-mail:wangqing87329@126.com
  • 基金资助:
    云南省应用基础研究项目资助 （2012FB106）

Mesenchymal stem cells transplantation alleviates pulmonary emphysema and oxidative stress in rat

  • Received:2014-09-26 Revised:2014-11-13 Published:2015-02-15 Online:2015-02-27

PDF (PC)

3862

摘要： 摘要： 目的 研究间充质干细胞 （MSCs） 移植在肺气肿大鼠氧化应激和肺气肿发展过程中的作用。方法 26 只雌性 SD 大鼠随机分为对照组 （A 组， n=8）， 肺气肿组 （B 组， n=8）， 肺气肿+MSCs 移植组 （C 组， n=10）， 将 B 与 C 组大鼠在香烟烟雾中暴露 14 周， 制作肺气肿大鼠模型， C 组大鼠予 MSCs 移植。完成 4 次 MSCs 移植后的 2、 4 周分别处死 C 组大鼠各 1 只， 观察 MSCs 在肺气肿大鼠肺部的定植情况； MSCs 移植 8 周后同时处死各组大鼠， 观察大鼠肺组织病理形态， 并进行定量分析； 用硫代戊巴比妥法测定血清和肺组织匀浆中丙二醛 （MDA） 的水平， 用 WST-1 法测定各组血清和肺组织匀浆中超氧化物歧化酶 （SOD） 的水平。结果 MSCs 移植后 2 周及 4 周， C 组大鼠肺组织内均可见标记过的 MSCs， 随着时间的推移， 肺组织内 MSCs 比例逐渐减少。B 组和 C 组大鼠的肺组织呈现肺气肿样改变， B 组和 C 组大鼠肺组织的平均内衬间隔 （MLI） 高于 A 组， 平均肺泡数 （MAN） 明显低于 A 组， B 组大鼠肺组织的 MLI 高于 C 组， MAN 低于 C 组 （P<0.05）。B 组和 C 组大鼠血清及肺组织中 MDA 高于 A 组， SOD 低于 A 组， B 组血清及肺组织中 MDA 高于 C 组， SOD 低于 C 组 （P<0.05）。结论 MSCs 移植可能通过降低氧化应激水平对大鼠肺气肿发挥治疗作用。

关键词: 肺气肿, 间质干细胞, 氧化性应激, 间质干细胞移植

Abstract: Abstract： Objective To test the effect of bone marrow mesenehymal stem cells (MSCs) transplantation on oxidative stress and the development of pulmonary emphysema in rats. Methods SD rats (n=26) were randomly divided into three groups： normal control group (group A, n=8)， emphysema group (group B, n=8) and emphysema+MSCs transplantation group (group C, n=10)．Rat models of emphysema was established by exposing rats to cigarette smoking for 14 weeks. Then rats of group C received MSCs transplantation. At the 14th and 28th days after 4 course of MSCs transplantations, one rat in group C was sacrificed at each time point and their lungs were preserved in frozen sections. Survival of MSCs in the lung tissues were observed by fluorescence microscopy. Eight weeks after transplantations, lung sections were stained by hematoxylin and eo⁃ sin (HE) to observe the morphological alterations．Mean linear intercept (MLI) and mean alveolar numbers (MAN) were also measured. Serum and lung malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity were also examined. Re⁃ sults At the 14th day and 28th day after transplantations of MSCs, MSCs successfully localized to lung and survived in rat models of emphysema. Emphysematous changes of lung tissues were observed in both group B and group C. MLI was higher while MAN was lower in group B and C than those in group A (P < 0.05). MLI and MDA levels in serum and lung were high⁃ er while MAN level and SOD activity were lower in group B than those in group C (P < 0.05)．MDA levels in serum and lung was higher while SOD activity was lower in group B and C than those in group A (P < 0.05). Conclusion MSCs transplanta⁃ tions can effectively alleviates pulmonary emphysema in rat models which might through reducing oxidative stress．

Key words: pulmonary emphysema, mesenchymal stem cells, oxidative stress； mesenchymal stem cell transplantation