天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (3): 267-269.doi: 10.11958/j.issn.0253-9896.2015.03.012

• 临床研究 • 上一篇    下一篇

miR-451 在胃癌组织中的表达及其临床意义

陈涛， 谢黎明， 唐海林   

  1. 湖南衡阳， 南华大学附属第一医院医疗服务中心 （邮编 421001）
  • 收稿日期:2014-08-15 修回日期:2014-11-04 出版日期:2015-03-15 发布日期:2015-03-15
  • 通讯作者: 陈涛 E-mail:176024235@qq.com
  • 作者简介:陈涛 （1979）， 男， 主治医师， 学士， 主要从事消化、 急诊医学研究
  • 基金资助:
    国家自然科学基金资助项目 （31100935）

The expression of miR-451 and its clinical significance in gastric cancer

CHEN Tao， XIE Liming， TANG Hailin   

  1. Medical Service Center, The First Affiliated Hospital of University of South China, Hengyang 421001, China

  • Received:2014-08-15 Revised:2014-11-04 Published:2015-03-15 Online:2015-03-15

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摘要： 目的 研究 miR-451 在胃癌组织中的表达并探讨其临床意义。方法 收集胃癌组织标本 92 例， 癌旁正常组织 47 例， 制成组织芯片。采用原位杂交技术检测胃癌组织及癌旁组织中 miR-451 的表达情况， 分析 miR-451 在胃癌组织中的表达水平与胃癌患者临床病理参数以及预后的关系。结果 miR-451 在胃癌组织中的高表达率比癌旁正常胃黏膜组织明显降低 （38.04% vs 76.60%， χ2=18.503， P＜0.001）。Ⅲ~Ⅳ期胃癌患者 miR-451 的高表达率低于Ⅰ~Ⅱ期胃癌患者（27.3% vs 54.1%， P=0.009）， 有淋巴结转移胃癌组织中 miR-451 的高表达率亦低于无淋巴结转移者 （27.1% vs 57.6%， P=0.004）， 而不同年龄、 性别、 组织分化程度、 T 分期患者间 miR-451 高表达率差异无统计学意义（P > 0.05）。miR-451 高表达者总生存率明显高于低表达者（69.2% vs 39.1%， P=0.003）。结论 miR-451 与胃癌的恶性转变、 浸润转移以及预后明显相关， 可能是胃癌新的潜在诊断和预后分子标志物及治疗靶点。

关键词: 胃肿瘤, 癌, 预后, 基因表达, 原位杂交, miR-451

Abstract: Objective To investigate the expression of miR-451 and its clinical significance in gastric cancer. Methods The miR-451 expression was detected in 92 samples of gastric cancer and 47 sapmles of normal stomach mucosa. The correlations of miR-451 expression with clinicopathologic characteristics, overall survival and disease-free survival were an⁃ alyzed in patients with gastric cancer. Results The overexpression rate of miR-451 was 38.04% in gastric cancer, which was significantly lower than 76.60% in adjacent normal tissues (χ2=18.503， P＜0.001). The overexpression rate of miR-451 was significantly lower in Ⅲ-Ⅳ stages of gastric cancer than that in Ⅰ-Ⅱ stages (27.3% vs 54.1%, P=0.009). The expres⁃ sion of miR-451 was also lower in patients with lymph node metastasis than that in patients without lymph node metastasis (27.1% vs 57.6%, P=0.004). However, there were no significant differences in miR-451 expressions between different age, gender, histological grade and T stage groups (P > 0.05). The overall survival rate was significantly higher in patients with high expression of miR-451 than that in patients with low expression of miR-451(69.2% vs 39.1%, P=0.003). Conclusion miR- 451 is associated with the malignant transformation, progression, and prognosis of gastric cancer, and which can be used as a potential target of therapy and a biomarker of diagnosis and prognosis of gastric cancer in the future.

Key words: stomach neoplasms, carcinoma, prognosis, gene expression, in situ hybridization, miR-451