天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (12): 1432-1435.doi: 10.11958/20160555

• 实验研究 • 上一篇    下一篇

激素治疗对阿霉素肾病大鼠上颌骨骨密度的影响

侯晓艳， 李孝英， 郭乐乐， 马明， 郭奕， 彭诚△   

  1. 天津医科大学第二医院口腔科 （邮编 300211）
  • 收稿日期:2016-06-15 修回日期:2016-08-10 出版日期:2016-12-15 发布日期:2017-01-26
  • 通讯作者: 彭诚 △通讯作者 E-mail： peng_cheng2013@163.com E-mail:hxy900202@163.com
  • 作者简介:侯晓艳 （1990）， 女， 硕士在读， 主要从事口腔颌面部错 畸形矫治方面研究
  • 基金资助:
    天津市卫生局科技基金项目 （2014KZ095）

Effects of glucocorticoids on maxillary bone mineral density in rat model of adriamycin-induced nephropathy

HOU Xiaoyan, LI Xiaoying, GUO Lele, MA Ming, GUO Yi, PENG Cheng△   

  1. Department of Stomatology, the Second Hospital of Tianjin Medical University, Tianjin 300211, China
  • Received:2016-06-15 Revised:2016-08-10 Published:2016-12-15 Online:2017-01-26
  • Contact: PENG Cheng △Corresponding Author E-mail:peng_cheng2013@163.com E-mail:hxy900202@163.com

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摘要： 摘要： 目的 研究甲泼尼龙激素治疗对阿霉素肾病大鼠上颌骨骨密度 （BMD） 的影响。方法 将 40 只大鼠随机分为 4 组， 空白对照组、 激素组、 阿霉素肾病组、 阿霉素+激素组。阿霉素肾病组及阿霉素＋激素组采用间隔 1 周 2 次尾静脉注射盐酸阿霉素 4 mg/kg 建立大鼠阿霉素肾病模型， 空白对照组和激素组尾静脉注射生理盐水 4 mg/kg。建模后激素组及阿霉素＋激素组给予甲泼尼龙 30 mg/ （kg·d） 灌胃， 空白对照组及阿霉素肾病组给予等体积生理盐水灌胃。每日 1 次， 连续 10 周。采用酶联免疫吸附试验 （ELISA） 测定骨钙素 （BGP）、 Ⅰ型原胶原 N-端前肽 （PINP）、 β- Ⅰ型胶原交联 C 末端肽 （CTX） 水平； 并行上颌骨 micro-CT 三维扫描检测骨小梁厚度 （Tb.Th）、 骨小梁间隙 （Tb.Sp）、骨小梁数目 （Tb.N）、 骨体积分数 （BVF）、 BMD。结果 与其余 3 组相比， 阿霉素＋激素组 BGP、 PINP 降低， CTX 升高（P＜0.05）。经 micro-CT 三维扫描分析， 阿霉素＋激素组上颌骨发生明显骨质疏松现象， 包括骨显微结构的改变， BMD 降低、 BVF 降低、 Tb.Th 减少以及 Tb.Sp 增宽 （P＜0.05）。但各组 Tb.N 差异无统计学意义。结论 阿霉素肾病大鼠本身存在骨代谢异常， 大剂量激素治疗可加速骨质疏松的发生， 使其骨代谢水平下降， 影响骨小梁结构。

关键词:  肾病综合征, 上颌骨, 骨密度, 糖皮质激素类, 骨质疏松, 大鼠, Wistar, 阿霉素肾病, micro-CT

Abstract: Abstract: Objective To evaluate effects of glucocorticoids on maxillary bone mineral density in rats with acute adriamycin- induced nephrotoxicity (ADR). Methods Forty rats were randomly divided into four groups, control group, glucocorticoids- treated group, ADR group and ADR + glucocorticoids- treated group. ADR group and ADR + glucocorticoids-treated group were given 4 mg/kg adriamycin injection via tail vein to establish ADR model. Control group and glucocorticoids- treated group were given 4 mg/kg saline injection via tail vein. After establishment of ADR model, glucocorticoids-treated group and ADR + glucocorticoids-treated group were intragastric administration of 30 mg/(kg ·d) methylprednisolone for 10 weeks, and control group and ADR group were given the same volumes of normal saline. Values of bone calcium pigment (BGP), type Ⅰ collagen, N- terminal pro- peptide (PINP), β-Ⅰ type collagen C- terminal cross- linked telopeptide (CTX) were detected by ELISA. The micro-CT scan was used to measure Tb.Th, Tb.Sp, Tb.N, BVF and bone mineral density (BMD). Results Compared with other three groups, the levels of BGP and PINP were significantly decreased, and CTX were significantly increased in ADR + glucocorticoids- treated group (P＜0.05). Micro-CT analysis showed that there was significant maxillae osteoporosis, including changes of porous micro architecture, lower BMD, decreased BVF, lower Tb.Th and widening Tb.Sp in ADR + glucocorticoids- treated group (P＜0.05). There was no significant difference in Tb.N between four groups. Conclusion There is imbalanced bone metabolism in rat model of ADR. High-dose hormone therapy can accelerate the occurrence of osteoporosis, decrease bone metabolism, and affect bone structure.

Key words:  nephrotic syndrome, maxilla, bone density, glucocorticoids, osteoporosis, rats, Wistar, adriamycin-induced nephrotoxicity, micro-CT