天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (10): 1021-1024.doi: 10.11958/20170626

• 实验研究 • 上一篇    下一篇

DC-GPC3 联合 CIK 对肝癌移植瘤的抑制作用

靳燕宇 1，王玉亮 2,3△，宋扬 1，杨涛 1   

  1. 1 天津市第一中心医院普外科（邮编 300192），2 检验科；3 天津市泌尿外科研究所
  • 收稿日期:2017-06-02 修回日期:2017-08-22 出版日期:2017-10-15 发布日期:2017-10-13
  • 通讯作者: 靳燕宇 E-mail:wyyjyy@126.com
  • 作者简介:靳燕宇（1983），男，主治医师，主要从事肝癌治疗研究
  • 基金资助:
    天津市卫生行业重点攻关项目;国家自然科学基金资助项目

Antitumor effect of DC-GPC3 cocultured with CIK on hepatocellular carcinoma in vivo

JIN Yan-yu1, WANG Yu-liang2,3△, SONG Yang1, YANG Tao1   

  1. 1 Department of General Surgery, 2 Department of Clinical Laboratory Medicine, Tianjin First Central Hospital, Tianjin 300192, China; 3 Tianjin Institute of Urology
  • Received:2017-06-02 Revised:2017-08-22 Published:2017-10-15 Online:2017-10-13
  • Supported by:
    Fund of Tianjin Municipal Bureau of Health

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摘要： 目的 探讨磷脂酰肌醇蛋白聚糖-3（GPC3）基因转染的树突状细胞（DC，DC-GPC3）与细胞因子诱导杀伤 细胞（CIK）共培养（DCIK-GPC3）后在体内能否抑制裸鼠皮下肝癌移植瘤生长。方法 将肝癌荷瘤裸鼠模型分为 4 组，A 组于瘤体周围注射生理盐水（NS），B 组于瘤体周围注射 CIK，C 组于瘤体周围注射 DC-CIK，D 组于瘤体周围注 射 DCIK-GPC3，比较 4 组肿瘤体积、抑瘤率和肿瘤细胞凋亡变化。结果 治疗后，D 组肿瘤组织的体积和质量明显 小于 A、B 及 C 组，差异有统计学意义（P＜0.01）；抑瘤率明显高于 B 组、C 组，差异有统计学意义（P＜0.01）。D 组肿 瘤细胞凋亡率与 A 组、B 组和 C 组相比显著增高，差异有统计学意义（P＜0.01）。结论 DCIK-GPC3 效应细胞可明 显抑制肝癌移植瘤的生长。

关键词: 磷脂酰肌醇蛋白聚糖类, 树突细胞, 细胞因子类, 杀伤细胞, 肝肿瘤

Abstract: Objective To investigate whether immunotherapy with dendritic cells (DC) transduced with glypican 3 (GPC3) (DC- GPC3) and cocultured with cytokine- induced killer cells (CIK) (DCIK- GPC3) is capable of inhibiting hepatocellular carcinoma (HCC) in vivo. Methods The hepatocarcinoma cell-bearing mouse models were established and divided randomly into four groups (A, B, C, D) for the injection of normal saline (NS), CIK, DC-CIK, and DCIK-GPC3, respectively. Changes of tumor size, tumor inhibitory rate and cell apoptosis were compared between four groups. Results After treatment, the tumor volumes and weights were significantly decreased in D group compared with those of A, B and C groups (P＜0.01). The inhibition rate was significantly increased in D group compared with that of B group and C group (P＜ 0.01). The apoptotic rate was significantly increased in D group compared with that of A group, B group and C group (P＜ 0.01). Conclusion DCIK-GPC3 can significantly inhibit tumor growth in vivo.

Key words: glypicans, dendritic cells, cytokines, killer cells, liver neoplasms