天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (2): 131-134.doi: 10.11958/20170897

• 细胞与分子生物学 • 上一篇    下一篇

miR-301b在调节脂肪细胞分化中的作用

徐晓薇1 ， 李芝佳2 ， 王宝利1△   

  1. 1天津医科大学代谢病医院内分泌研究所， 卫生部激素与发育重点实验室 （邮编300070）； 2天津市人民医院血管疾病诊疗中心
  • 收稿日期:2017-08-14 修回日期:2017-12-04 出版日期:2018-02-15 发布日期:2018-02-15
  • 通讯作者: 徐晓薇 E-mail:megxxw@foxmail.com
  • 基金资助:
    miR-196B对成骨细胞和脂肪细胞分化的调节作用及其机制研究

The role of miR-301b in the regulation of adipocyte differentiation

XU Xiao-wei 1 , LI Zhi-jia2 , WANG Bao-li 1△   

  • Received:2017-08-14 Revised:2017-12-04 Published:2018-02-15 Online:2018-02-15

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摘要： 摘要： 目的 研究 miR-301b 在调节间充质干细胞向脂肪细胞分化过程中的作用。方法 对小鼠骨髓基质细胞 ST2 进行脂肪细胞诱导分化， 并利用 qRT-PCR 检测细胞分化过程中成脂分化诱导组相对于阴性对照组的 miR-301b 表达变化。对 ST2 细胞转染 miR-301b mimics 并进行成脂诱导分化， 利用 qRT-PCR 和 Western blot 技术检测 miR- 301b mimics 转染组和阴性对照片段 （NC） 转染组细胞的脂肪特异性基因和蛋白表达水平的变化。结果 成脂分化诱导组 miR-301b 相对表达水平 （0.219±0.021） 较对照组 （1.000±0.425） 减少 （P＜0.05）。miR-301b mimics 转染组的脂肪细胞特异性转录因子过氧化物酶体增殖物激活受体 （PPARγ）、 CCAAT 增强子结合蛋白 （C/EBPα） 和脂肪型脂肪酸结合蛋白 （aP2） 的相对表达量均较 NC 转染组降低 （P＜0.05）。miR-310b mimics 转染组与 NC 转染组相比， 标志基因aP2、 转录因子PPARγ 和C/EBPα 蛋白的表达量均减少 （P＜0.05）。结论 miR-301b可抑制脂肪细胞分化。

关键词: 关键词： 微RNAs, RNA 干扰, 间质干细胞, miR-301b, 脂肪分化

Abstract: Abstract: Objective To examine the effect of miR-301b in the regulating the differentiation of mesenchymal stem cells into adipocytes. Methods Murine ST2 stromal cells were isolated, cultured and induced with adipogenic agents. The expression of miR-301b was detected by qRT-PCR in adipogenic differentiation group and control group. Stromal ST2 cells were transfected with miR-301b mimics followed by adipogenic treatment. qRT-PCR and Western blotting were conducted to detect the changes of adipocyte specific genes and protein expression levels in the miR-301b mimics transfection group and negative control (NC) transfection group. Results qRT-PCR showed that the expression of miR-301b was decreased after adipogenic treatment in ST2 cells. The relative expression level of miR-301b was less in the adipogenic differentiation group (0.219 ± 0.021) than that of the control group (1.000 ± 0.425, P＜0.05). The relative expressions of peroxisome proliferator activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα) and adipocyte fatty acid binding proteins (aP2) were lower in the miR-301b mimics transfecting group than those in the NC transfecting group (P＜0.05). The protein levels of the marker gene aP2 and transcription factors PPARγ and C / EBPα decreased in miR-310b mimics transfecting group compared with those of the NC transfecting group (P＜0.05). Conclusion miR-301b can reduce adipocyte differentiation.

Key words: Key words: microRNAs, RNA interference, mesenchymal stem cells, miR-301b, adipogenesis