Abstract: Abstract: Objective To investigate the effect of assemble flavone of rhizome drynaria (AFRD) on the migration and regulation of SDF1 / CXCR4 signaling pathway in murine bone marrow stromal ST-2 cell line. Methods AFRD was obtained from qianggu capsule by ethanol ultrasonic extraction, CCK-8 method was used to determine the effects of AFRD (5, 20, 50 and 100 mg / L) on the proliferation of ST-2 cells. Transwell was used to detect the effect of AFRD on ST-2 migration ability. Fluorescence quantitative PCR and Western blot assay were used to detect the effect of AFRD on SDF1 and CXCR4 gene expressions. After intervention with CXCR4 inhibitor AMD3100, the migration ability and gene expression of SDF1 and CXCR4 regulated by AFRD in ST-2 cells were observed. Results AFRD promoted the migration of ST-2 cells and up-regulated the expressions of SDF1 and CXCR4 genes in ST-2 cells in a concentration-dependent manner. AMD3100, a CXCR4 inhibitor, can reverse the effect of AFRD on ST-2 cell migration and the upregulation of SDF1 and CXCR4 gene expression. Conclusion AFRD can promote ST-2 migration, which is mediated by SDF1/CXCR4 signaling pathway.

Key words: Drynaria Fortunei, cell movement, chemokine CXCL12, bone marrow stromal cells, SDF1, CXCR4