Table of Content

15 October 2019, Volume 47 Issue 10 Previous Issue    Next Issue

---

For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Select

Assemble flavone of rhizome drynaria promoted the migration of mouse ST-2 cells through SDF-1/CXCR4 signaling pathway WU Zi-xuan, HAO Zheng, GUO Ya-ping, ZHANG Zhai-yi, CHEN Lu-yao, PENG Yan-fei, ZHENG Fang 2019, 47 (10):  1009-1014.  doi: 10.11958/20192305 Abstract ( 552 )   PDF (999KB) ( 3609 )   Abstract: Objective To investigate the effect of assemble flavone of rhizome drynaria (AFRD) on the migration and regulation of SDF1 / CXCR4 signaling pathway in murine bone marrow stromal ST-2 cell line. Methods AFRD was obtained from qianggu capsule by ethanol ultrasonic extraction, CCK-8 method was used to determine the effects of AFRD (5, 20, 50 and 100 mg / L) on the proliferation of ST-2 cells. Transwell was used to detect the effect of AFRD on ST-2 migration ability. Fluorescence quantitative PCR and Western blot assay were used to detect the effect of AFRD on SDF1 and CXCR4 gene expressions. After intervention with CXCR4 inhibitor AMD3100, the migration ability and gene expression of SDF1 and CXCR4 regulated by AFRD in ST-2 cells were observed. Results AFRD promoted the migration of ST-2 cells and up-regulated the expressions of SDF1 and CXCR4 genes in ST-2 cells in a concentration-dependent manner. AMD3100, a CXCR4 inhibitor, can reverse the effect of AFRD on ST-2 cell migration and the upregulation of SDF1 and CXCR4 gene expression. Conclusion AFRD can promote ST-2 migration, which is mediated by SDF1/CXCR4 signaling pathway. References | Related Articles | Metrics

Select

Prediction and validation of bioinformatic effects of HOTAIR on miR-152 targeting HLA-G LI Xiao-juan, ZHOU Zhi-yi, WANG Jue, QIAN Yuan 2019, 47 (10):  1014-1019.  doi: 10.11958/20190638 Abstract ( 545 )   PDF (689KB) ( 4062 )   Abstract: Objective To predict and validate the targeting regulation of HOX antisense RNA (HOTAIR) on human leukocyte antigen-G (HLA-G) by upregulating miR-152-3p. Methods Bioinformatics websites and software were used to predict the binding sites of miR-152-3p targeting to HOTAIR and HLA-G 3′untranslated region (UTR). The 3′-UTR and mutant sequences of HOTAIR and HLA-G genes containing the binding sites with miR-152-3p were designed and synthesized, and the wild-type and mutant double luciferase reporter gene vectors of HOTAIR and HLA-G were constructed. After two-enzyme digestion electrophoresis and sequencing, HOTAIR and HLA-G wild-type and mutant double luciferase reporter gene vectors were co-transfected HTR-8/SVneo cells with miR-152-3p mimics and microRNA negative sequence control (mimics NC) respectively. Luciferase activity was detected, and the effects of miR-152-3p on the expression of HOTAIR and HLA-G were observed. Results The results of double enzyme digestion electrophoresis and sequencing showed that the size and sequence of wild type and mutant type HOTAIR and HLA-G gene vectors were consistent with the experimental expectations, and the vectors were successfully constructed. After transfection of miR-152- 3p mimics, the activity of HOTAIR and HLA-G wild-type luciferase decreased significantly (P＜0.05), but the expression of luciferase in mutant HOTAIR and HLA-G vectors was not significantly inhibited (P＞0.05). Conclusion HOTAIR regulates HLA-G targeting by upregulating the expression of miR-152-3p. References | Related Articles | Metrics

Select

Galangin enhanced antitumor effects of apatinib in gastric cancer SGC-7901 cells via PI3K/Akt and p38-MAPK signaling pathway HE Wen-yu, ZHANG Hai-ming, YUAN Chang-jin 2019, 47 (10):  1020-1025.  doi: 10.11958/20190272 Abstract ( 752 )   PDF (1425KB) ( 3718 )   Abstract: Objective To explore the influence of galangin in the antitumor effect of apatinib in gastric cancer cells and the underlying mechanism. Methods Human gastric cancer cell line SGC-7901 was cultured in vitro, and the satisfactory concentration of galangin and apatinib against SGC-7901 cells were determined by MTT assay. Gastric cancer cells were assigned to four groups: blank control group, galangin group, apatinib group and galangin+ apatinib group. Cell proliferation was determined by MTT assay, cell apoptosis was determined by flow cytometry assay, the migration and invasion of SGC-7901 cells were detected by wound healing and Transwell assay, respectively. Meanwhile, the expressions of related proteins were measured by Western blot assay. Results Both galangin and apatinib could significantly inhibit the proliferation of SGC-7901 cells in a dose-dependent manner (P＜0.05). Compared with apatinib group there was an enhanced inhibitory effects on cell proliferation, migration and invasion in galangin+apatinib group (P＜0.05). There was a significant promoted apoptosis in the galangin + apatinib group compared with that of 20 mg/L apatinib group (P＜0.05). Western blot showed that the expression levels of Bax, p-Akt and p-p38 were increased, and the level of Bcl-2 protein was significantly decreased, in galangin + apatinib group comparison with those of 20 mg / L apatinib group (P＜0.05). Conclusion Galangin can enhance antitumor effects of apatinib on gastric cancer SGC-7901 cells by inhibiting PI3K/Akt and activating p38-MAPK signaling pathway. References | Related Articles | Metrics

Select

Mechanism of interleukin-4 negatively regulating NF-κB pathway to inhibit inflammatory response HUANG Jie-yuan, LIU Wen-ming 2019, 47 (10):  1025-1029.  doi: 10.11958/20191391 Abstract ( 786 )   PDF (450KB) ( 4135 )   Abstract: Objective To investigate the effect of interleukin (IL) -4 on MyD88/NF-κB signaling pathway in Ana-1 cells induced by lipopolysaccharides (LPS). Methods The mouse macrophages Ana-1 were divided into LPS group (given 50 μg/L LPS stimulation) and LPS+IL-4 group (given LPS stimulation after 10 μg/L IL-4 pre-culture for 1 hour). Cell culture supernatants were collected at 0, 0.5, 1 and 2 hours. The relative expression levels of MyD88 and NF-κB mRNA were detected by RT-PCR. The expression levels of MyD88, total protein of NF - κB and NF - κB p65 were detected by Western blot assay. The content ratio in nucleus and cytoplasm of NF-κB p65 and the contents of interleukin (IL)-6 and tumor necrosis factor (TNF)-α in cell culture supernatant were detected by ELISA. Results With the prolongation of cell culture time, the expression levels of MyD88 and NF-κB mRNA and proteins, the ratio of NF-κB p65 nuclei/cytoplasm, IL- 6 and TNF-a levels increased gradually (P＜0.05). There was no significant difference in the expression of MyD88 in LPS+ IL-4 group (P＞0.05). The expression of MyD88 protein, NF-κB mRNA and protein, the nuclear/cytoplasmic ratio of NF- κB p65 and levels of IL-6 and TNF-α increased first and then decreased (P＜0.05). The expression of MyD88 protein, the nuclear/cytoplasmic ratio of NF-κB p65 and levels of IL-6 and TNF-α at 1 h and 2 h were significantly decreased in LPS+ IL-4 group than those of LPS group (P＜0.05). There were no significant differences in the expression levels of NF- κB mRNA and proteins in different time points between two groups (P＞0.05). Conclusion IL-4 may play an antiinflammatory role by inhibiting the activation of MyD88/NF-κB signaling pathway. IL-4 can down-regulate the expression of pro-inflammatory cytokines IL-6 and TNF-α. References | Related Articles | Metrics

Select

High phosphorus promotes calcification of vascular smooth muscle cells via SET8 regulating p53/Bcl-2/Caspase signaling pathway ZHANG Dong-xue, LU Jing 2019, 47 (10):  1030-1034.  doi: 10.11958/20191487 Abstract ( 594 )   PDF (858KB) ( 3564 )   Abstract: Objective To explore the role and mechanism of histone lysine methyltransferase SET8 in the calcification of vascular smooth muscle cells (VSMCs) induced by high phosphorus. Methods The clean male SD rats weighting 80 g to 100 g were selected, and their thoracic aortae were taken for primary culture. VSMCs were passed to the 3rd to 4th generation, and the cells were plated into six-well plates, and then VSMCs were given corresponding stimulation. VSMCs were randomly divided into normal group and high phosphorus group (10 mmol/L β- glycerophosphate). The formation of calcium nodules was observed after four-day culture. Flow cytometry was used to detect the apoptosis of cells. The expression levels of mRNA and protein of SET8, p53, Bcl-2, Bax and Caspase3 were detected by RT-PCR and Western blot assay. Then SET8-shRNA plasmid was transfected into VSMCs that was used as SET8-shRNA group. The un-transfected group was used as normal control group. The effects of SET8 on mRNA and protein expression levels of p53, Bcl-2 Bax and Caspase3 were detected by RT-PCR and Western blot assay. Results (1) The calcium deposition was significantly increased in the high phosphorus group compared with that of normal group (P＜0.05). (2) The result of flow cytometry showed that compared with the normal group, the apoptosis rate of VSMCs was significantly increased in the high phosphorus group (P＜0.05). (3) Compared with the normal group, the expression levels of Bcl-2 and SET8 mRNA and protein were significantly decreased, and the expression levels of p53, Bax and Caspase3 increased in the high phosphorus group (P＜0.05). (4) Calcium salt deposition increased after interference with SET8 (P＜0.05). The expression level of Bcl-2 decreased and the expression levels of p53, Bax and Caspase3 increased after interference with SET8 gene expression (P＜0.05). Conclusion High phosphorylation regulates p53 / Bcl-2 / Caspase signaling pathway through SET8, down-regulates the expression of anti-apoptotic protein Bcl-2, up-regulates the expressions of pro-apoptotic proteins Bax and Caspase3, and then participates in the regulation of vascular smooth muscle cell calcification. References | Related Articles | Metrics

Select

The effect of knockdown of glucagon gene on insulinoma formation ZHU Guo-ling, CHENG Lan-yun, ZHANG Heng, TIAN Hao, MEN Xiu-li 2019, 47 (10):  1035-1039.  doi: 10.11958/20181998 Abstract ( 651 )   PDF (1046KB) ( 3963 )   Abstract: Objective To discuss the effect of knockdown of glucagon gene on insulinoma formation by using cells in vitro and animal model of insulinoma. Methods The expressions of insulin and glucagon proteins in INS-1 cells were observed by immunofluorescence staining and laser scanning confocal microscopy. Then INS-1 cells were infected with lentivirus pLVTHM-glucagon to knock down the glucagon gene. Therefore, an INS-1 cell line with low glucagon expression was established. Cells were divided into blank control group, lentivirus transfection group and glucagon knockdown group. The cell proliferation was detected by MTT assay. The ability to release insulin and glucagon was detected using ELISA and radioimmunity. And in vivo tumorigenicity of INS-1 cells was evaluated after glucagon gene knockdown. Expression levels of insulin and glucagon of tumor tissue were further detected by immunohistochemistry staining. Results Knockdown of glucagon gene impaired proliferation of INS-1 cells. The release of glucagon, but not insulin was decreased after glucagon knockdown. Subrenal capsul assay showed significantly reduced speed of neoplasia of INS-1 cells, with markedly decreased glucagon gene expression in this insulinoma tissue, which was displayed by immunohistochemical staining. Conclusion The knockdown of glucagon gene can inhibit proliferation of insulinoma cells, and release of glucagon, further restrain insulinoma formation. The result indicates that glucagon gene expression may be an important factor of the insulinoma formation. References | Related Articles | Metrics

Select

Total flavones of bidens bipinnata L. alleviated cognitive impairment in rats with focal cerebral ischemia through ERK1/2/NF-κB pathway LI Li-li, JIAO Fu-ying 2019, 47 (10):  1040-1045.  doi: 10.11958/20190667 Abstract ( 647 )   PDF (561KB) ( 4301 )   Abstract: Objective To investigate the effect of total flavones of bidens bipinnata L. (TFB) on cognitive function in rats with focal cerebral ischemia and its possible mechanism. Methods Seventy-two rats were randomly divided into sham operation group, model group, positive control group (1 mg/kg nimodipine), low-dose TFB group (25 mg/kg), medium-dose TFB group (50 mg/kg) and high-dose TFB group (100 mg/kg). The rat model of focal cerebral ischemia was established by modified thread embolization. Morris water maze test was used to test the learning and memory abilities of rats. The histopathological changes of hippocampus were observed by Nissl staining. The contents of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in hippocampus were detected by enzyme-linked immunosorbent assay. Western blot assay was used to detect the expressions of ERK1/2, p-ERK1/2, NF-κB p65 and p-NF-κB p65 in hippocampus. Results Compared with the sham-operated group, the average escape latency increased, the number of crossing plateau decreased, the contents of BDNF and NGF in hippocampus decreased and the contents of IL-1β, IL-6 and TNF-α, and the expressions of p-ERK1/2 and p-NF-κB p65 protein increased in the model group (P＜0.05). Compared with model group, the average escape latency of rats decreased after the second day in high dose TFB group and positive control group (P＜0.05). The number of times of rats crossing the platform increased in middle and high dose TFB groups and positive control group, and the contents of BDNF and NGF in hippocampus were also increased (P＜0.05). The contents of IL-1β, IL-6 and TNF-α and the expression levels of p-ERK1/ 2 and p-NF-kappa B p65 were all decreased in a dose-dependent manner (P＜0.05). There were no significant differences in detection indexes between positive control group and high-dose TFB group (P＞0.05). Conclusion TFB can improve cognitive function in rats with focal cerebral ischemia, and its mechanism may be related to the inhibition of ERK1/2/NF-κB signaling pathway. References | Related Articles | Metrics

Select

The role of CDK5-mediated PPARγ phosphorylation in the formation of foam cells in atherosclerosis SHEN Na, HE Jing, DI Yan-bo, LIU Yong, TIAN Feng-shi, LIU Yun-de 2019, 47 (10):  1045-1049.  doi: 10.11958/20190295 Abstract ( 908 )   PDF (451KB) ( 4165 )   Abstract: Objective To investigate the role of cyclin-dependent kinases 5 (CDK5) - mediated PPARγ phosphorylation in atherosclerosis. Methods Raw264.7 macrophages were induced by ox-LDL to differentiate into foam cells, and then CDK5 inhibitor was used as the intervent. The experimental cells were divided into control group (C group), ox-LDL group (O group, 50 mg/L ox-LDL) and roscovitine group (50 mg/L ox-LDL+15 μmol/L Roscovitine). Expressions of pPPARγ, tPPARγ, p35 and CDK5 protein were detected by Western blot assay. Lipid accumulation was analyzed by oil red O staining and isopropyl alcohol extraction experiments. Levels of cholesterol content in macrophages were measured by enzymatic method. Expression of ox-LDL uptake related genes CD36, SR-A1 and cholesterol efflux related genes ABCA1 and ABCG1 were detected by RT-PCR. Results After ox-LDL induction, the ratios of pPPARγ/tPPARγ and p35/CDK5 significantly increased, and lipid accumulation, total cholesterol content, free cholesterol content and the ratio of cholesterol ester / total cholesterol were up-regulated significantly in O group compared with those of C group (P＜0.05). RT-PCR showed that expression levels of ox-LDL uptake-related genes CD36 and SR-A1 mRNA increased, while the expression levels of cholesterol efflux-related genes ABCA1 and ABCG1 decreased in O group (P＜0.05). After intervention with CDK5 inhibitor, the above indicators showed the opposite trend compared with those of O group (P＜0.05). Conclusion CDK5/ pPPARγ pathway is involved in the formation of foam cells in atherosclerosis. References | Related Articles | Metrics

Select

Clinical value of MUC1 protein in early diagnosis of non-small cell lung cancer FU Hong-yan, WANG Yi-yong, ZHANG Ke-dong, ZHOU Feng 2019, 47 (10):  1050-1053.  doi: 10.11958/20191449 Abstract ( 972 )   PDF (612KB) ( 4124 )   Abstract：Objective To investigate the expression of MUC1 protein in serum and cancer tissues in patients with different types of non-small cell lung cancer (NSCLC), and to analyze its predictive value in early clinical diagnosis and treatment of NSCLC patients. Methods From December 2017 to December 2018, samples of peripheral blood and lung tissues of 34 cases of lung squamous cell carcinoma, 65 cases of lung adenocarcinoma and 20 cases of non-lung cancer were collected from the General Hospital of Ningxia Medical University. The serum expression of MUC1 protein was detected by enzyme-linked immunosorbent assay (ELISA). After HE staining confirmed that the lung tissue specimens met the pathological requirements, MUC1 protein expression was detected by immunohistochemical staining. The clinical value of serum MUC1 protein for predicting different types of NSCLC patients was performed by ROC curve analysis. Results The expressions of MUC1 protein in serum and cancer tissues were significantly higher in lung squamous cell carcinoma group and lung adenocarcinoma group than those of non-lung cancer group (P＜0.05). The optimum critical value of serum MUC1 protein in the diagnosis of lung squamous cell carcinoma was 33.23 U/mL, with a sensitivity of 52.94% and a specificity of 100%. The positive expression rates of MUC1 proteins in serum and cancer tissues of patients with lung squamous cell carcinoma were not related to the differences of pathological differentiation, TNM stage, age and sex (P＞0.05). The optimal critical value of serum MUC1 protein in the diagnosis of lung adenocarcinoma was 31.31 U/mL, with a sensitivity of 61.54% and a specificity of 95%. The positive expression rates of MUC1 proteins in serum and cancer tissues were significantly higher in lung adenocarcinoma patients with middle-low tissue differentiation and TNM stage Ⅲ-Ⅳ than those of patients with high tissue differentiation and TNM stage Ⅰ-Ⅱ(P＜0.05). Conclusion MUC1 protein is highly expressed in NSCLC patients, which may be of predictive significance for early diagnosis and treatment of lung adenocarcinoma, and has no obvious prognostic significance for lung squamous cell carcinoma. References | Related Articles | Metrics

Select

The prognostic value of NLR combined with procalcitonin in patients with bacterial infection of acute exacerbation of COPD LUO De-yun, CHEN Ju-ping 2019, 47 (10):  1054-1057.  doi: 10.11958/20190925 Abstract ( 690 )   PDF (379KB) ( 3919 )   Abstract：Objective To investigate the predictive value of neutrophil-lymphocyte ratio (NLR) combined with procalcitonin (PCT) in the death of patients with bacterial infection of acute exacerbation of chronic obstructive pulmonary disease (COPD). Methods Data of 210 patients with bacterial infection (sputum culture found bacteria at least twice) of acute exacerbation of COPD were analyzed retrospectively. The patients were divided into survival group (n=188) and nonsurvival group (n=22) according to the survival status at the time of discharge. Clinical data and laboratory results were compared between the two groups. The risk factors for death was analyzed by Logistic regression model, and predictive values of NLR, PCT and NLR combined with PCT in the death of patients with bacterial infection of acute exacerbation of COPD were analyzed by receiver operating characteristic (ROC) curve. Results Compared with survival group, the white blood cell count (WBC), neutrophil count (NEU), NLR, PCT, C-reactive protein (CRP) and arterial blood carbon dioxide partial pressure [ p(CO2) ] were significantly higher in non-survival group, while pH value and albumin were significantly lower in non-survival group (P＜0.05). Logistic regression analysis showed that the elevated NLR (OR=1.329, 95%CI:1.106-1.597) and PCT (OR=1.306, 95% CI:1.012-1.685) were high risk factors for death in patients. The ROC curve showed that the area under the curve (AUC) of the NLR, PCT, predictive regression model of NLR combined with PCT were greater than 0.7. And their specificities were 0.739, 0.564, and 0.702, the sensitivities were 0.727, 0.909, and 0.909. Conclusion Both NLR and PCT have preferably good predictive values for death in patients with bacterial infection of acute exacerbation of COPD. The predictive value is higher when the NLR and PCT were evaluated together. References | Related Articles | Metrics

Select

Analysis of the efficacy of extremely early application of noninvasive ventilator in patients with acute heart failure REN Kai, CHEN Shu-tao, FENG Jin-ping, GENG Jie 2019, 47 (10):  1058-1062.  doi: 10.11958/20191087 Abstract ( 723 )   PDF (395KB) ( 4878 )   Abstract: Objective To analyze extremely early application of noninvasive ventilator in patients with acute heart failure. Methods A total of 100 patients with acute heart failure who were admitted and treated in Cardiac Intensive Care Unit (CICU) from January 2017 to December 2018 were studied retrospectively. Patients were divided into the extremely early application group (n=44) and the control group (n=56). Patients of two groups were given nasal tube oxygenation to reduce cardiac load, control heart rate and blood pressure, and improve myocardial metabolism after the onset of acute heart failure. On the basis of routine treatment, the application group was given extremely early application (10-30 min) of noninvasive ventilator assisted ventilation, and the control group was given noninvasive ventilator assisted ventilation in 30 min -24 h after onset. There were no significant differences in gender, clinical symptoms and vital signs before treatment between the two groups (P＞0.05). Data of respiratory rate (RR), resting heart rate (HR), mean arterial pressure (MAP), arterial blood oxygen partial pressure [p(O2)], arterial blood carbon dioxide partial pressure [p(CO2)] and lactic acid (Lac) were monitored before and 24 h after treatment. The serum levels of N-terminal B-brain natriuretic peptide (NT-proBNP) were also recorded before and after 1 week of treatment. The total efficacy rate was compared between the two groups. Results There were no significant differences in the related indicators before treatment between two groups of patients. The RR, HR, MAP and p(O2) were significantly improved after 24 h-treatment in the extremely early application group than those of control group (P＜0.05). Values of p(CO)2, Lac and NT-proBNP were significantly decreased in the extremely early application group than those of the control group (P＜0.05). The total efficacy rates were 90.91% in the early application group and 82.14% in the control group. There was no significant difference between the two groups (P＞0.05). Conclusion The extremely early application (10-30 min) of noninvasive ventilator assisted therapy has significant effect on patients with acute heart failure. References | Related Articles | Metrics

Select

Trend of T-SPOT.TB in the effective process of early antituberculosis treatment of tuberculosis LI Xiao-fei, LIU Hong-wei, LUO Feng, QIU Wan 2019, 47 (10):  1062-1066.  doi: 10.11958/20191021 Abstract ( 682 )   PDF (419KB) ( 3659 )   Abstract：Objective To study the change trend of T-cell spot of Tuberculosis (T-SPOT.TB) in the effective process of early anti-tuberculosis treatment of tuberculosis. Methods Fifty newly diagnosed patients with active pulmonary tuberculosis who received regular anti-tuberculosis treatment in tuberculosis department of our hospital from June 2016 to February 2019 were selected as subjects of the study. The dynamic change trend of T-SPOT.TB results and the changes of body's response to tuberculosis specific antigen [early secreting antigen target-6 (ESAT-6), culture filtrate protein-10 (CFP- 10)] stimulation before and after anti-tuberculosis treatment (30 days, 1 month and 3 months) were analyzed. Results During the period of effective anti-tuberculosis treatment of three months, the results of T-SPOT.TB decreased gradually with the prolongation of treatment time, and the difference was statistically significant (P＜0.05). The changes of immune response to ESAT-6 and CFP-10 were consistent after treatment for three months. Conclusion T-SPOT.TB may be a good indicator for monitoring the efficacy of tuberculosis. The changes of body response to ESAT-6 and CFP-10 are consistent after anti-tuberculosis treatment (at the 3rd month). References | Related Articles | Metrics

Select

Efficacy analysis of two different noninvasive ventilation strategies in neonatal respiratory distress syndrome ZHU Liu-jie, REN Guang-li, XIE Cong, ZHANG Qing-mei, XIE Guo-qiang 2019, 47 (10):  1067-1072.  doi: 10.11958/20191858 Abstract ( 583 )   PDF (389KB) ( 3713 )   Abstract：Objective To compare the clinical efficacy of nasal intermittent positive pressure ventilation combined with nasal continuous positive airway pressure (NIPPV+NCPAP) and nasal continuous positive airway pressure (NCPAP) in the treatment of neonatal respiratory distress syndrome. Methods A total of 102 newborns with respiratory distress syndrome (RDS) who were admitted between January 2018 to June 2019 were randomly divided into two groups NIPPV+ NCPAP group (n=50) and NCPAP group (n=52). The differences in the improving respiratory rate, oxygen and reduce lactic acid value, ventilation failure, related complications were compared in newborns with RDS between NIPPV+NCPAP group and NCPAP group. According to the ventilation outcome, all the newborns were divided into failed ventilation group (n=34) and successful ventilation group (n=68). The effects of inhaled oxygen volume fraction (FiO2) and lactic acid on the outcome of ventilation were analyzed in the two groups. Results The reducing breath rate, increasing oxygen partial pressure and improving oxygen at 1 h, 6 h, and 12 h were significantly better in NIPPV+NCPAP group than those of NCPAP group (all P＜ 0.05). The lactic acid value decreased with ventilation time in NIPPV+NCPAP group. After ventilation for 12 h, the lactate value was significantly lower in NIPPV + NCPAP group than that of NCPAP group (P＜0.05). The ventilation failure and apnea also reduced in NIPPV+NCPAP group than those of NCPAP group (all P＜0.05). There were no significant differences  in non-invasive ventilation time, no oxygenation time after non-invasive surgery, total intestinal feeding time, hospitalization time, hospitalization expenses, and complications such as chlorination of chloral hydrate due to irritability, feeding intolerance, necrotizing enterocolitis, sepsis, patent ductus arteriosus, intracranial hemorrhage, and bronchopulmonary dysplasia between the two groups of newborns (all P＞0.05). Compared with the successful ventilation group, the FiO2 ≥ 0.30 within 2 hours after birth and lactic acid value continued to increase obviously more in the ventilation failure group (all P＜ 0.05). Conclusion The noninvasive ventilation strategy with NIPPV combined with NCPAP is safe for neonatal respiratory distress syndrome, which is effective and worth promoting.   References | Related Articles | Metrics

Select

Metabolic evaluation for patients with Randall’s plaque urolithiasis ZHANG Yi-fan, XU Chang-bao, WAN You, WANG Xi-kun, ZHOU De-zhi, JI Ming-yang, WANG You-ming 2019, 47 (10):  1072-1075.  doi: 10.11958/20190128 Abstract ( 634 )   PDF (536KB) ( 3569 )   Abstract：Objective To investigate the stone risk factors in patients with Randall’s plaques by analyzing the 24-h urinary metabolic analysis. Methods From October 2017 to October 2018, 30 patients with Randall’s plaques, 20 patients with no - Randall’s plaques and 30 healthy people were collected, and 24-h urine samples were analyzed. The stone components were analyzed by infrared spectroscopy. Results There were no significant differences in size, location, side and composition of the stones between Randall’s plaque group and no-Randall’s plaque. The content of citric acid decreased, while the contents of oxalic acid and phosphoric acid increased in Randall’s plaque group than those of control group (P＜0.05). In Randall plaque group, the content of citric acid in 24-h urine decreased, while the contents of oxalic acid, phosphoric acid, sodium and calcium increased (P＜0.05). Compared with no-Randall’s plaque group, the contents of citric acid in 24 h urine decreased, and the contents of oxalic acid, phosphoric acid, sodium and calcium increased in Randall plaque group (P＜0.05). Conclusion The high sodium, calcium, oxalic acid, phosphoric acid and low citric acid in 24-hour urine of patients with Randall’s plaques have important effects on the occurrence and development of urolithiasis. The intake of oxalic acid, sodium, calcium and phosphoric acid should be limited and citric acid should be supplemented in patients with Randall’s plaque urolithiasis. References | Related Articles | Metrics

Select

The efficiency of sacubitril/valsartan therapy in patients with chronic heart failure JIA Hong-dan, CONG Hong-liang, ZHAO Yun-feng, BI Xi-le, SONG Ting-ting, GUO Qiang-hua, WANG Qi, LIU Li 2019, 47 (10):  1076-1080.  doi: 10.11958/20191271 Abstract ( 1190 )   PDF (371KB) ( 5160 )   Abstract: Objective To observe the clinical efficacy of sacubitril / valsartan in patients with chronic heart failure. Methods Eighty-eight patients with chronic heart failure were enrolled in this study. They were divided into angiotensin receptor enkephalinase inhibitor (ARNI, n=47) group and control group (n=41). ARNI group was treated with sacubitril / valsartan, while control group received benazepril hydrochloride or valsartan. Data of body weight, 24-hour urine volume, dosage of diuretics, N-terminal pro-brain natriuretic peptid (NT-proBNP) level before treatment and 1 month after treatment, and cardiac structure and function before treatment and three months after treatment were compared between the two groups respectively. Results In ARNI group,the body weight, diuretic dosage and NT-proBNP level decreased, 24- hour urine volume increased, while left ventricular end-diastolic diameter (LVDD) and left atrium (LA) reduced, stroke volume (SV), cardiac output (CO), left ventricular ejection fraction (LVEF) and mitral diastolic blood flow velocity E peak and A peak ratio (E / A) increased after treatment compared to the baseline (all P＜0.05). Data of the NT-proBNP level decreased, 24-hour urine volume increased, LVDD and LA reduced, SV, LVEF and E/A increased (all P＜0.05) in ARNI group after treatment compared with those of the control group (P＜0.05). Cardiac function also improved in ARNI group after treatment compared to that of the control group (P＜0.05). Conclusion Compared with benazepril / valsartan, sacubitril/valsartan can further reduce water-sodium retention and improve cardiac function. References | Related Articles | Metrics

Select

A randomized controlled study of extracorporeal shock wave combined with hyperbaric oxygen in the treatment of nonunion ZHAO Zi-xing, LI Hong-yu, YIN Dong, LIANG Bin, LYU Ya-nan, PAN Xiao-rong, HUANG Qing, HUANG Si-cheng, WEI Ming-zhao, WANG Hong-run 2019, 47 (10):  1081-1084.  doi: 10.11958/20191177 Abstract ( 705 )   PDF (371KB) ( 3770 )   Abstract: Objective To investigate the efficacy of extracorporeal shock wave therapy (ESWT) combined with hyperbaric oxygen for treatment of long-bone fracture nonunion. Methods Sixty cases of long bone fracture nonunion in our hospital were used as study subjects. They were divided into three groups by random number table method. Group A was treated by ESWT，group B was treated by ESWT combined with hyperbaric oxygen and group C was treated by surgery. All patients were followed up 12 months at least. Then the healing rate (6, 9 and 12 months), bone densities (6, 12 and 24 weeks), alkaline phosphatase (ALP, 1, 2, 3, 4, and 6 weeks) and complications were observed in three groups of patients. Results All patients had no long-term complications, and the skin complications were less in group B than those in group A and C. There were no significant differences in the effective rates and ALP levels at different time points after treatment between group B and C, and which were better than those of group A (P＜0.05). The ALP levels were higher at different time points after treatment than those before treatment in three groups, and the overall trend was increased with time (P＜0.05). The bone densities at different time points were higher after treatment in group B than those of group A(P＜0.05). The bone mineral densities at each time point were higher in group A and group B than those before treatment, and the overall trend was increased with time (P＜0.05). Conclusion ESWT combined with hyperbaric oxygen for treatment of long-bone fracture nonunion is safe, effective and have a fewer skin complications. References | Related Articles | Metrics

Select

Study on the application value of serum tumor abnormal protein combined with urine cytokeratin-19 detection in the early diagnosis of bladder cancer WU Wan-wen, LYU Cai, LIU Zhen-xiang, XU Hai-xia, ZHANG Shu-fang 2019, 47 (10):  1085-1088.  doi: 10.11958/20190460 Abstract ( 721 )   PDF (406KB) ( 4326 )   Abstract: Objective To investigate the application value of serum tumor abnormal protein (TAP) combined with urine cytokeratin-19 (CK-19) detection in the early diagnosis of bladder cancer. Methods Fifty patients with bladder cancer confirmed by pathology (bladder cancer group) and 45 patients with benign diseases of urinary system (control group) were retrospectively analyzed. The serum levels of TAP and urine CK-19 levels were detected by agglutination method and enzyme linked immunoabsorbent assay in the two groups, respectively. Binary Logistic regression analysis was used to establish a new diagnostic index for TAP and CK-19 combined detection. The ROC curve was drawn to analyze the diagnostic value of TAP and CK-19 separate or combined detection for bladder cancer. The diagnostic efficacy of TAP and CK-19 alone and combined detection was calculated. Results TAP and CK-19 levels were significantly higher in bladder cancer group than those in control group (P＜0.01). The area under the ROC curve detected by TAP combined with CK-19 [0.918 (95%CI: 0.864-0.973)] was higher than that by TAP alone [0.876 (95%CI: 0.809-0.944)] and CK-19 alone [0.821 (95%CI: 0.727-0.915)]. The Youden index of the joint diagnosis of the two indicators (0.83) was also higher than the two separate indicators (TAP: 0.61; CK-19: 0.64), and the sensitivity (90.00%) and specificity (93.33%) of the combined diagnosis of the two indicators were greater than the single indicator (TAP: 70.00%/91.11%; CK-19: 84.00%/80.00%). Conclusion Combined detection of TAP and CK-19 can improve the diagnostic value and efficacy of single indicator detection and can be used as a new indicator for the early diagnosis of bladder cancer. References | Related Articles | Metrics

Select

Malignant lymphoma complicated with podocytic infolding glomeruopathy after chemotherapy: a case report LI Juan, LU Wan-jun, GONG Shu-hao, WANG Ying 2019, 47 (10):  1089-1091.  doi: 10.11958/20190627 Abstract ( 744 )   PDF (906KB) ( 3570 )   Abstract: Podocytic infolding glomerulopathy (PIG) is a rare glomerular disease found in recent years. Its diagnosis mainly depends on pathological features. At present, there are relatively few clinical cases of this disease. This paper introduces a case of PIG caused by chemotherapy of malignant lymphoma. A 46-year-old female patient showed positive proteinuria and mild edema of lower limbs after chemotherapy for diffuse large B lymphoma. The related experimental data were in accordance with the diagnosis of nephrotic syndrome. The renal biopsy was performed. The pathological diagnosis was in consistent with PIG. The purpose of this article was to analyze the pathogenesis of PIG and review the clinical reports of PIG. References | Related Articles | Metrics

Select

A case of successful rescued pregnancy patient with spinal deformity and heart failure NAN Lian-ling, LI Jie, ZHANG Hong, BAI Yu-fang 2019, 47 (10):  1092-1095.  doi: 10.11958/20191225 Abstract ( 741 )   PDF (461KB) ( 3803 )   Abstract: Pregnancy with heart disease is the most common cause of non-direct obstetric death and the second highest risk factor for maternal mortality. Because of the normal physiological changes of pregnant women, pregnancy with heart failure usually occurs in the 32-34 weeks of gestation, the delivery period and three days after delivery. For pregnant women with potential risk of heart failure, it is very important to pay close attention to the vital signs of patients, to choose the suitable time and mode to terminate pregnancy for the prognosis of patients. We report a 27-year-old patient with a short height of 1.45 meters. At the age of two, severe kyphoscoliosis was caused by trauma. Later, due to repeated upper respiratory tract infections, pectus carinatum was formed. In late stage of the pregnancy, early stage of heart failure appeared and developed to grade Ⅲ of NYHA heart failure. Finally, The pregnancy was terminated by cesarean section to help the patient give birth, both mother and child were healthy in six months of follow up. References | Related Articles | Metrics

Select

Effect analysis of short-term comprehensive intervention in patients with type 2 diabetes mellitus complicated with overweight or obesity WANG Hui, WANG Jiao, LIU Hong-wei, LU Rui, WANG Meng-yang, LI Chang-ping, LIU Yuan-yuan, MA Jun 2019, 47 (10):  1096-1101.  doi: 10.11958/20191410 Abstract ( 667 )   PDF (374KB) ( 3530 )   Abstract: Objective To explore the effects and influencing factors of short-term comprehensive intervention on blood glucose control in obese or overweight patients with type 2 diabetes mellitus. Method: Baseline questionnaire survey (including gender, age, diabetes-related information), physical measurement (height, weight, waist circumference, etc.) and biochemical tests (including fasting blood glucose (FPG), postprandial 2H blood glucose (2h PBG) and glycosylated hemoglobin (HbA1c)) were conducted in 47 hospitals in Tianjin in 2018. Biochemical indicators after 3 months of intervention were included. χ^2 test, univariate and multivariate unconditional logistic regression were analyzed by using SAS statistical package. Results Among 987 diabetic patients with overweight or obesity, 738 (74.8%) were obese and 872 (88.3%) were central obese. There were significant differences in blood sugar indexes before and after comprehensive intervention (P < 0.001); the effective rate of 3-month intervention was 65.75% (649/987). Univariate unconditional logistic regression analysis showed that age, course of disease and regularity of three meals were statistically significant. (P < 0.001); Multivariate logistic regression analysis showed that normal diet was an effective protective factor for 3-month comprehensive intervention (OR = 1.992, 95% CI: 1.466-2.706), course of disease (> 10 years) (OR (95% CI): 10-15 years: 0.555 (0.37-0.831); course (> 15 years: 0.546 (0.357-0.834)) and age (> 60 years) (OR = 0.604, 95% CI: 0.404-0.904) are risk factors for effective 3-month comprehensive intervention. Conclusion Comprehensive intervention for obese and overweight patients with T2DM can effectively improve the standard rate of glycosylated hemoglobin and reduce the blood sugar levels of patients. At the same time, it is suggested to establish and improve the comprehensive intervention system to improve the blood sugar standard rate of this population. References | Related Articles | Metrics

Select

Research progress on relationship between intestinal flora and obesity and type 2 diabetes YUAN Bing-shu, ZHAO Hai-long, LI Li-juan 2019, 47 (10):  1102-1107.  doi: 10.11958/20191036 Abstract ( 771 )   PDF (394KB) ( 3757 )   Abstract: Intestinal flora is the microflora colonized in the intestine. Intestinal flora microecological balance can be affected by various factors, including living environment, life style, eating habits and drugs. These changes are the pathological basis of many diseases. Increasing evidence shows that intestinal flora including Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes, can increase the incidence of obesity and type 2 diabetes (T2DM) through immune, metabolic and circadian clock pathways. These researches have become the focus of academic discussion. This article summarizes the relationship between changes in intestinal flora and obesity and T2DM in recent years, in order to further clarify the pathogenesis of obesity and T2DM, and provide some new ideas for finding therapeutic targets. References | Related Articles | Metrics

Select

The current research status and application prospects of CD326 in malignant tumors ZHOU Jing, DING Wen-wen, ZHU Yu-xi 2019, 47 (10):  1107-1111.  doi: 10.11958/20182217 Abstract ( 1250 )   PDF (350KB) ( 3806 )   Abstract: CD326 is a transmembrane protein, which is expressed in some normal epithelial cells and participates in the formation of human embryo and the differentiation of the adult cells. Also CD326 is expressed in the epithelial carcinoma cells and plays an important role in the formation and spread of tumors. Since its discovery, CD326 has attracted much attention in the expression, significance, mechanism, diagnosis and treatment in various common malignant tumors. This paper discussed the structure feature of CD326, the relationship between CD326 and the tumor microenvironment, the significance of CD326 expression in normal stem cells and tumor cells, and the clinic application, in order to further study the application value of tumor-related molecules. References | Related Articles | Metrics

Select

Progress in the application of sonodynamic therapy in atherosclerosis XIE Qian, ZHAO Yun, LIU Rong 2019, 47 (10):  1112-1116.  doi: 10.11958/20191138 Abstract ( 812 )   PDF (378KB) ( 4001 )   Abstract: Atherosclerosis is a chronic vascular disease with high morbidity and mortality. Although the current treatment has achieved certain therapeutic effects, it has the characteristics of long treatment cycle, large adverse reactions and invasiveness. Sonodynamic therapy (SDT) has become a hot topic in current research because of its non-invasive nature, less impact on normal tissues, strong tissue penetration and no significant attenuation of energy. SDT can cause cell apoptosis and autophagy, improve lipid deposition, reduce inflammatory cell infiltration, promote vascular smooth muscle cell repopulation and prevent matrix degradation, and it is a potential treatment for atherosclerosis. The effects of SDT on atherosclerosis and its mechanisms are summarized below. References | Related Articles | Metrics

Select

Advances in application of extracorporeal circulation of centrifugal pumps JI Zhen-hua, WU Ting 2019, 47 (10):  1117-1120.  doi: 10.11958/20191109 Abstract ( 1091 )   PDF (330KB) ( 3716 )   Abstract: Centrifugal pumps have more and more clinical applications as a new generation of cardiopulmonary bypass (CPB) power device because of its small size, light weight, high safety coefficient, large flow rate and minor damage to blood components. At present, centrifugal pumps are widely used in extracorporeal membrane oxygenation (ECMO), left heart assist and conventional CPB heart surgery in foreign hospitals. In recent years, although there have been reports on the clinical application of centrifugal pumps in China, most of them are used for ECMO, and the majority of conventional CPB heart surgery still uses rolling pump as the driving blood pump. In this paper, the current situation, clinical application, advantages and precautions of centrifugal pump research at home and abroad are reviewed in order to better guide clinical work. References | Related Articles | Metrics