Abstract: Single cell sequencing is a powerful new sequencing technology, which can analyze the genome, transcriptome and epigenome in a single cell. Based on single-cell sequencing, the different molecular and clinicopathological characteristics of tumor heterogeneity can be explored in colorectal cancer, which provide important insights into the early diagnosis, therapy and prognosis of colorectal cancer. This paper reviewed the progress of single-cell sequencing technology in colorectal cancer, so as to provide references for the identification of prognostic markers of colorectal cancer, the search for new therapeutic targets and and personalized therapy.

Key words: colorectal neoplasms, single cell sequencing, intratumoral, treatment