Tianjin Medical Journal

Tianjin Medical Journal ›› 2022, Vol. 50 ›› Issue (6): 561-565.doi: 10.11958/20212403

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Tetrahydroxystilbene glucoside and emodin improves the hippocampal neuronal apoptosis in mice induced by high glucose

CHEN Gang1, 2, XU Yongjie1, 3, HUANG Changyudong1, 2, LIN Hairong1, 2, YANG Tingting1, 2, ZHU Liying4, #br# LI Xing5, PAN Wei1, 3△#br#   

  1. 1 The Affiliated Hospital of Guizhou Medical University, Guizhou Prenatal Diagnosis Center, Guiyang 550004, China; 
    2 School of Clinical Laboratory Science, Guizhou Medical University; 3 School of Public Health, Guizhou Medical 
    University; 4 Clinical Laboratory Center, the Affiliated Hospital of Guizhou Medical University; 
    5 Guizhou University of Traditional Chinese Medicine
  • Received:2021-10-25 Revised:2022-02-24 Published:2022-06-15 Online:2023-12-20

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Abstract: Abstract: Objective　To explore the effect of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D- glucoside (TSG) and Emodin, the main components of polygonum multiflorum, on the apoptosis of hippocampal neuron HT-22 cells induced by high glucose. Methods　The mouse hippocampal neuron HT-22 cells were divided into 4 groups: the control medium group (NC group, glucose concentration 25 mmol/L), the high-glucose medium group (HG group, glucose concentration 55 mmol/L), the HG+TSG group and the HG+Emodin group. The HG+TSG group and the HG+Emodin group were treated with TSG and Emodin at the concentrations of 50, 100 and 200 μmol/L for 12, 16, 20, 24 and 48 h, respectively. The cell viability was detected by CCK-8 method after treatment. Histone acetyltransferase (HAT) and histone deacetylase (HDAC) enzyme activities were detected by enzyme-linked immunosorbent assay (ELISA). Flow cytometry was used to detect the apoptosis rate. Western blotting was used to detect the expression of apoptosis-related proteins: B-lymphoma-2 (Bcl-2), Bcl-2-related X protein (Bax) and Caspase-3. Results　The 200 μmol/L of TSG for 48 h, and 100 μmol/L of Emodin for 48 h were the optimal condition for culturing cells after testing. Flow cytometry results showed that TSG and Emodin significantly down-regulated cell apoptosis rate after 48 h of treatment. ELISA results showed that the activity of HAT was significantly down-regulated, HDAC activity was up-regulated in the HG+TSG group. The activities of HAT and HDAC were significantly down-regulated in the HG+Emodin group. Western blot results showed that the expressions levels of Bax and Caspase-3 were significantly down-regulated, and the expression of Bcl-2 was significantly up-regulated after TSG and Emodin treated cells for 48 h. Conclusion　TSG and Emodin may regulate the activities of HAT and HDAC, thereby down-regulate the apoptosis of hippocampal neurons induced by high glucose.

Key words: hippocampus, neurons, emodin, apoptosis, histone deacetylases, fallopia multiflora, bcl-2-associated X protein, proto-oncogene proteins c-bcl-2, caspase 3, diabetes encephalopathy