Tianjin Medical Journal

Tianjin Med J ›› 2015, Vol. 43 ›› Issue (6): 674-676.doi: 10.11958/j.issn.0253-9896.2015.06.026

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Study of miR-125b, miR-29a and miR-155-5p as biomarkers of tuberculous meningitis

CHAI Lulu1,TIAN Songxin1,YUAN Li2 ,WANG Hong1   

  1. 1 Department of Neurology , the First Affiliated Hospital,Medicine School of Shihezi University, Shihezi 832000,China;
    2 Department of Pathogenic Microbiology and Immunology,Medicine School of Shihezi University

  • Received:2014-12-01 Revised:2015-01-22 Published:2015-06-15 Online:2015-06-10

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Abstract: Objective To investigate the expression levels of microRNA(miR)-125b, miR-29a and miR-155-5p in cerebrospinal fluid (CSF) and plasma from the patients with tuberculous meningitis and their clinic significance. Methods Cerebrospinal fluid and plasma samples were collected from 20 patients with tuberculous meningitis (tuberculous meningitis group) and 20 patients suffered from primary headache (control group). The total RNAs were extracted.The levels of miR-
125b, miR-29a and miR-155-5p were determined by real-time quantitative PCR (q-PCR). Results Levels of miR-29a and miR-125b in both CSF and plasma of patients in tuberculous meningitis group were significantly higher than those in patients from control group with statistical significance (P<0.01). Mean time, the level of miR-155-5p in plasma but not in CSF of patients in tuberculous meningitis group was higher than those in control group with statistical significance (P<0.01). Conclusion Expression of miR-125b, miR-29a and miR-155-5p may participate in regulating the occurrence and development of tuberculous meningitis. And miR-125b and miR-29a may be used as potential biomarkers for diagnosing tuberculous meningitis.

Key words: tuberculosis, meningeal, plasma, cerebrospinal fluid, microRNAs, real-time quantitative PCR, miR-125b,
miR-29a, miR-155-5p