Analgesic effect of total saponins of Clematis on trigeminal neuralgia rats by regulating the miR-26a-5p/Wnt5a signaling pathway

doi:10.11958/20251039

Abstract

Abstract:

Objective To investigate the analgesic effect of total saponins of Clematis (TSC) on trigeminal neuralgia (TN) rats by regulating the miR-26a-5p/Wnt5a signaling pathway. Methods The TN rat model was established through suborbital nerve constriction surgery. Fifty rats were divided into the TN group, the different doses of (50, 200 mg/kg) TSC groups, the TSC+NC antagonist (200 mg/kg TSC+5 nmoL NC antagonist) group and the TSC+miR-26a-5p antagonist (200 mg/kg TSC+5 nmoL miR-26a-5p antagonist) group2025， with 10 mice per group. Another 10 rats were taken as the sham operation group, and only the infraorbital nerve of rats was exposed but not ligated. ELISA was used to detect the levels of tumor necrosis factor (TNF) -α and interleukin (IL) -6. HE staining was used to detect histopathological changes. Real-time fluorescence quantitative reverse transcription polymerase chain reaction was used to detect the expression levels of miR-26a-5p and Wnt5a mRNA. Western blot assay was used to detect protein expressions of glial neurotrophic factor (GDNF), Wnt5a and brain-derived neurotrophic factor (BDNF) in trigeminal ganglion tissue. Dual luciferase reporter gene system was applied to validate the targeting relationship between miR-26a-5p and Wnt5a. Results Compared with the sham operation group, the expression levels of TNF-α, IL-6, Wnt5a protein and mRNA were obviously increased in the TN group, and the mechanical pain threshold, the expression levels of miR-26a-5p, GDNF and BDNF were obviously reduced (P<0.05). Compared with the TN group, the expression of TNF-α, IL-6, Wnt5a protein and mRNA were greatly reduced in the 50 and 200 mg/kg TSC groups, and the mechanical pain threshold, the expression levels of miR-26a-5p, GDNF and BDNF were greatly increased. There were differences in different doses of TSC between the different groups (P<0.05). Inhibition of miR-26a-5p reversed the protective effect of TSC on TN rats. Conclusion TSC exerts analgesic effects on TN rats by regulating the miR-26a-5p/Wnt5a signaling pathway.

Key words: trigeminal neuralgia, analgesia, total saponins of Clematis, miR-26a-5p/Wnt5a signaling pathway

CLC Number:

R745.11

Figures/Tables 8

References 26

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组别	TNF-α	IL-6
假手术组	86.34±8.82	53.82±5.42
TN组	183.26±18.44^a	105.24±10.82^a
50 mg/kg TSC组	138.09±13.97^b	78.24±7.99^b
200 mg/kg TSC组	92.05±9.31^bc	58.64±5.93^bc
TSC+NC antagomir组	91.52±9.22	59.34±6.08
TSC+miR-26a-5p antagomir组	136.52±13.75^d	77.82±7.88^d
F	90.175^＊＊	64.326^＊＊

组别	TNF-α	IL-6
假手术组	86.34±8.82	53.82±5.42
TN组	183.26±18.44^a	105.24±10.82^a
50 mg/kg TSC组	138.09±13.97^b	78.24±7.99^b
200 mg/kg TSC组	92.05±9.31^bc	58.64±5.93^bc
TSC+NC antagomir组	91.52±9.22	59.34±6.08
TSC+miR-26a-5p antagomir组	136.52±13.75^d	77.82±7.88^d
F	90.175^＊＊	64.326^＊＊

组别	Wnt5a mRNA	miR-26a-5p
假手术组	0.98±0.10	1.02±0.11
TN组	2.01±0.21^a	0.25±0.03^a
50 mg/kg TSC组	1.51±0.16^b	0.54±0.06^b
200 mg/kg TSC组	1.15±0.12^bc	0.85±0.09^bc
TSC+NC antagomir组	1.09±0.11	0.88±0.09
TSC+miR-26a-5p antagomir组	1.49±0.16^d	0.61±0.07^d
F	65.780^＊＊	125.119^＊＊

组别	Wnt5a mRNA	miR-26a-5p
假手术组	0.98±0.10	1.02±0.11
TN组	2.01±0.21^a	0.25±0.03^a
50 mg/kg TSC组	1.51±0.16^b	0.54±0.06^b
200 mg/kg TSC组	1.15±0.12^bc	0.85±0.09^bc
TSC+NC antagomir组	1.09±0.11	0.88±0.09
TSC+miR-26a-5p antagomir组	1.49±0.16^d	0.61±0.07^d
F	65.780^＊＊	125.119^＊＊

组别	GDNF/β-actin	BDNF/β-actin	Wnt5a/β-actin
假手术组	0.86±0.09	1.02±0.11	0.42±0.05
TN组	0.22±0.03^a	0.35±0.04^a	1.38±0.15^a
50 mg/kg TSC组	0.58±0.06^b	0.65±0.07^b	0.95±0.10^b
200 mg/kg TSC组	0.78±0.08^bc	0.83±0.09^bc	0.72±0.08^bc
TSC+NC antagomir组	0.76±0.08	0.91±0.10	0.67±0.07
TSC+miR-26a-5p antagomir组	0.49±0.05^d	0.68±0.07^d	1.03±0.11^d
F	120.925^＊＊	80.538^＊＊	114.387^＊＊