Tianjin Medical Journal

Tianjin Medical Journal ›› 2015, Vol. 43 ›› Issue (7): 724-727.doi: 10.11958/j.issn.0253-9896.2015.07.006

• Cell and Molecular Biology • Previous Articles     Next Articles

Liensinine promotes apoptosis of bladder cancer 5637 cells and enhances Caspase-7 expression and activation

XU Bin, ZHANG Tianyu, TAN Ning, YAN Qi, XU Lingli, LIU Xiaojia, GAO Li   

  1. 1 Department of Urology, Affiliated Hospital of Guilin Medical University, Guilin 541004, China; 2 Center of Science Research, Guilin Medical University; 3 Department of Gastroenterology, 4 Department of Pathology, Affiliated Hospital of Guilin Medical University
  • Received:2015-01-15 Revised:2015-04-28 Published:2015-07-15 Online:2015-07-15
  • Contact: ZHANG Tianyu E-mail： zhangtianyu64@qq.com E-mail:461387614@qq.com

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Abstract: Objective To study the effects of Liensinine on apoptosis of 5637 cells, and its mechanism thereof. Methods CCK-8 method and the colony formation test were used to detect cell viabilities, and then inhibition rates were calculated. Flow cytometry was used to detect the effects of Liensinine on apoptosis of 5637 cells. Western blot assay was used to detect Caspase-7 protein expression. Results CCK-8 assay and colony formation test indicated that Liensinine inhibited the cell proliferation significantly. Results of flow cytometry indicated that Liensinine induced early apoptosis of 5637 cells. Western blot assay showed that Liensinine improved the expression of Caspase-7 and enhanced the activation of Caspase-7 in 5637 cells. Conclusion Liensinine could inhibit the proliferation of 5637 cells, induce early apoptosis, which may be related with the enhanced expression of Caspase-7 and its activation.

Key words: urinary bladder neoplasms, alkaloids, cell proliferation, apoptosis, cysteine endopeptidases, liensinine, Caspase-7