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15 July 2015, Volume 43 Issue 7 Previous Issue    Next Issue
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Cell and Molecular Biology
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Cell and Molecular Biology

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Differential expressions of surface-associated proteins of streptococcus mutans strains isolated from clinical samples under acid condition ZAHO Xingfu, JIANG Shan, HUANG Xiaojing, YAN Fuhua 2015, 43 (7):  705-709.  doi: 10.11958/j.issn.0253-9896.2015.07.001 Abstract ( 550 )   PDF (686KB) ( 3580 )   Objective To investigate the changes in the surface-associated protein expression of streptococcus rnutans (Sm) isolated from clinical samples at pH7.0 and pH5.0. Methods The proteins were extracted from cells at pH7.0 and pH5.0 by Homer method. The proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) and two-dimensional gel electrophoresis followed by image analysis. Proteins were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry and computer-assisted protein sequence analysis. Results Image analysis revealed that four high expression levels of protein loci and two specific protein loci were existed in two strains at pH7.0. Two high expression levels of protein loci and two specific protein loci were existed in two strains at pH5.0. Three high expression levels of protein loci and six specific protein loci were existed in Sm593 at pH5.0. Two-component system histidine kinases Lyts and glyceraldehyde- 3- phosphate dehydrogenase were highly modulated, and NADH oxidase were modulated specifically. Conclusion The two clinical isolations in acid have high expression of some special proteins, which are presumed to be the resemblance of acid reaction．The difference of protein expression of two clinical isolations in acid may represent their distinct acid resistance． Related Articles | Metrics

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Fabrication and biocompatibility assessment of a silk fibroin scaffold with biomimetic oriented microstructure for cartilage tissue engineering YANG Qiang, DING Xiaoming, XU Baoshan, ZHAO Yanhong, LIU Yue, ZHANG Yang, MA Xinlong 2015, 43 (7):  709-712.  doi: 10.11958/j.issn.0253-9896.2015.07.002 Abstract ( 791 )   PDF (831KB) ( 3657 )    Objective To fabricate a silk fibroin scaffold with biomimetic oriented microstructure using the directional crystallization technology, and to evaluate the possibility of application to the cartilage tissue engineering. Methods The silk fibroin scaffold with biomimetic oriented microstructure was made by the directional crystallization technology. The structure of scaffold was observed by the scanning electron microscope, and the pore size, porosity and mechanical properties were calculated. Adipose-derived stem cells were isolated from rabbit, and the passage 3 was seeded into the scaffold. The cell proliferation was detected by CCK8 method. The cell adhesion ability was observed by HE staining and scanning electron microscope. The cell viability was observed by LIVE/DEAD staining. Results The scanning electron microscopy showed that the parallel microtubulelike structure can be seen arranged in longitudinal section of the scaffold, which had a uniform directivity, and also the elliptical or circular pore structure in cross-section. The scaffold had a good pore interconnectivity with (112.43±12.65) µm pore diameter of the cross-section, (90.25±2.05)% porosity and (52.48±5.78) kPa the compression modulus. HE staining and scanning electron microscopy demonstrated that the cells uniformly adhered to the surface and inner pore, and which secreted lots extracellular matrix distributed in the oriented microstructure. Results of CCK8 showed that the good cell proliferation on the scaffold, and the LIVE/DEAD staining indicated that the cells were maintained good viability. Conclusion The silk fibroin scaffolds have a biomimetic oriented microstructure, and show good pore diameter, porosity, biocompatibility and biomechanical properties, which made it a suitable candidate as an alternative scaffold for cartilage tissue engineering. Related Articles | Metrics

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Study on differentiation pluripotency of human gingival fibroblasts induced in vitro JIANG Shaoyun, TAO Yufei, LI Yang, SONG Liting, ZHU Dongwang, DENG Jiayin 2015, 43 (7):  713-716.  doi: 10.11958/j.issn.0253-9896.2015.07.003 Abstract ( 956 )   PDF (707KB) ( 3915 )   Objective To investigate the pluripotency of human gingival fibroblasts (hGFs), and provide a novel cell source for tissue engineering. Methods With informed consent from volunteers, fresh and healthy gingiva were collected. The hGFs were obtained from the gingiva by tissue culture. The third passage of hGFs was cultured in osteogenic medium, chondrogenic medium and adipogenic medium. Cells without differentiation were taken as control. Cells were examined by alkaline phosphatase (ALP) staining, Alizarin red staining, Alcian blue staining and oil red O staining for detecting of the ability of differentiation pluripotency. Real-time polymerase chain reaction was applied to examine the expression of osteogenic marker genes ALP, runt-related transcript factor 2 (Runx2), chondrogenic marker aggrecan (AGR) and adipogenic marker peroxisome proliferator-activated receptor gamma 2 (PPARγ2).Results The hGFs cultured in osteogenic medium showed massive violet deposit at day 7 and calcium nodulus at day 28, meanwhile, the expressions of ALP and Runx2 were higher than those of control (P < 0.01). In chondrogenic group cells were found blue deposit at day 14. In adipogenic group lipidfilled droplets stained with oil red O were found in cells at day 14. However, hGFs in control group had no any positive staining. Furthermore, expressions of AGR and PPARγ2 were significantly higher than those of control (P < 0.01). Conclusion Human gingival fibroblasts have the pluripotency of osteogenic, adipogenic and chondrogenic differentiation. Related Articles | Metrics

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Establishment of miR-223 knockdown lentivirus ZHANG Xin, GUAN Xiaohui, WANG Baoli 2015, 43 (7):  717-720.  doi: 10.11958/j.issn.0253-9896.2015.07.004 Abstract ( 1067 )   PDF (543KB) ( 3803 )   Objective To construct miR-223 knockdown lentivirus vector and provide a tool for further study of the function of miR-223. Methods According to the Invitrogen miR-RNAi online design tool, a pair of complementary oligonucleotides encoding miR-223 mature sequence was designed, annealed and ligated with pcDNA6.2-GW/EmGFP-miR vector. Then miR-RNAi expression cassette was cut and subcloned into lentiviral pCDH-CMV-MCS-EF1-copGFP vector. The lentiviruses were packaged and titered, and then ST2 cells were infected with viruses. The efficiency of infection was calculated, and the knockdown of endogenous miR-223 was detected by using real-time RT-PCR. Results Restriction enzyme digestion and sequencing results showed that miR- 223 lentivirus construct was successfully made. Lentivirus that knockdown miR-223 expression packaged and infected of target cells. The expression of GFP green fluorescent protein accounted for 80%-90% and the virus titer was 1×109 PFU/mL. The infection efficiency reached 90%. Compared with negative control virus, miR-223 knockdown lentivirus significantly down-regulated the expression of miR-223 in ST2, and was 31% (n=3, t=15.091, P＜0.05). Conclusion miR-223 knockdown lentivirus is successfully made. It provides a tool for further studying the function of miR-223. Related Articles | Metrics

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Protective effects of penehyclidine hydrochloride on A549 cells against oxidative injury DU Xueke, PAN Linghui, PEI Shenglin, GE Wanyun 2015, 43 (7):  721-723.  doi: 10.11958/j.issn.0253-9896.2015.07.005 Abstract ( 613 )   PDF (280KB) ( 3550 )   Objective To explore the effects of penehyclidine hydrochloride (PHC) on lung epithelial type Ⅱ cells against oxidative stress damage. Methods A549 cells treated with H2O2 were used as oxidative stress damage cell model. A549 cells were divided into 3 groups: control group (C group), H2O2 treated group (H group) and PHC treated group (P group). The viability of A549 cells was measured by MTT assay. The apoptotic rate was measured by TUNEL assay. The levels of malonicdialed (MDA), reactive oxygen species (SOD), reduced glutathione hormone (GSH) and nicotinamide adenine dinucleotide phosphate (NADPH) of cells were detected by biochemistry colorimetry. Results Compared with group C, the viability of A549 and the contents of SOD, GSH and NADPH were significantly decreased in group H, while MDA content and apoptotic rate were increased (P＜0.05). Compared with group H, the viability of A549, the contents of SOD, GSH and NADPH were significantly increased in group P, while MDA content and apoptotic rate were reduced (P＜0.05). Conclusion Penehyclidine hydrochloride shows protective effects on A549 cells through reducing the oxidative damage induced by H2O2. Related Articles | Metrics

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Liensinine promotes apoptosis of bladder cancer 5637 cells and enhances Caspase-7 expression and activation XU Bin, ZHANG Tianyu, TAN Ning, YAN Qi, XU Lingli, LIU Xiaojia, GAO Li 2015, 43 (7):  724-727.  doi: 10.11958/j.issn.0253-9896.2015.07.006 Abstract ( 936 )   PDF (2511KB) ( 3821 )   Objective To study the effects of Liensinine on apoptosis of 5637 cells, and its mechanism thereof. Methods CCK-8 method and the colony formation test were used to detect cell viabilities, and then inhibition rates were calculated. Flow cytometry was used to detect the effects of Liensinine on apoptosis of 5637 cells. Western blot assay was used to detect Caspase-7 protein expression. Results CCK-8 assay and colony formation test indicated that Liensinine inhibited the cell proliferation significantly. Results of flow cytometry indicated that Liensinine induced early apoptosis of 5637 cells. Western blot assay showed that Liensinine improved the expression of Caspase-7 and enhanced the activation of Caspase-7 in 5637 cells. Conclusion Liensinine could inhibit the proliferation of 5637 cells, induce early apoptosis, which may be related with the enhanced expression of Caspase-7 and its activation. Related Articles | Metrics

Experimental Study

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The effects of liraglutide on learning and memory in Alzheimer-like triple transgenic mice with type 2 diabetes SUN Jie, CHEN Shuyi, LU Susu, ZHENG Jun, DENG Yanqiu 2015, 43 (7):  728-732.  doi: 10.11958/j.issn.0253-9896.2015.07.007 Abstract ( 848 )   PDF (530KB) ( 3796 )   Objective To investigate the effects of type 2 diabetes on learning and memory of APP/PS1/Tau triple transgenic (3×Tg) mice of Alzheimer’ s disease, and the protective mechanism of liraglutide (LIR) thereof. Methods One month old C57BL/6 mice were set to be control group (WT). One month old 3×Tg mice were divided into control group (Tg), liraglutide group (Tg+LIR), type 2 diabetes group (Tg+T2DM) and liraglutide treatment group (Tg+T2DM+LIR). The model of T2DM was established by feeding the high fat and sugar fodder, and then injecting streptozotocin (STZ) in mice, making sure the fasting blood glucose was more than 7 mmol/L. Then the subcutaneous injection of LIR was administered for 2 months. The values of body weight and fasting blood glucose were detected at age of 5-month. Morris water maze was applied to evaluate the spatial learning and memory ability. Western blotting assay was used to measure the levels of phosphorylated Tau, neurofilament (NFs) and insulin receptor substrates. ELISA was used to detect the human Aβ 42 to evaluate the effect of LIR on -amyloid. Results LIR can reduce body weight and blood glucose, can alleviate spatial learning and memory damaging caused by T2DM, and also can improve phosphorylated Tau levels, NFs and insulin receptor substrates caused by T2DM, and finally can reduce the deposition of β-amyloid of 3×Tg mice. Conclusion T2DM can aggravate symptoms of AD in 3×Tg mice, and LIR has a protective effect on it. Related Articles | Metrics

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Development of a duplex fluorescence RT-PCR assay for identifying SRBⅠ gene knockout mice PAN Lili, ZHENG Lu, ZHANG Jun, YU Yang, YAO Shuang, YU Miaomei, FENG Yuehua, LUO Guanghua 2015, 43 (7):  732-734.  doi: 10.11958/j.issn.0253-9896.2015.07.008 Abstract ( 887 )   PDF (379KB) ( 3814 )   Objective To develop a duplex fluorescence RT-PCR assay for detection of scavenger receptor class B, typeⅠ(SRBⅠ) knockout mice. Methods Primers and probes were designed according to knockout region of SRBⅠ gene and related substituted sequence. DNA samples were extracted from tails of mice and performed amplification using realtime PCR. SRBⅠ genotypes of mice were analyzed according to amplification curves of FAM and CY5 channels. Finally, the sensitivity of the method was detected and the accuracy was verified by the direct sequencing. Results The homozygous SRBⅠ wild genotype showed an amplification curve only in FAM channel. When the homozygous SRBⅠ knockout genotype was present, the typical S amplification curve appeared only in the CY5 channel. Heterozygous genotype showed two typical S amplification curves in both FAM and CY5 channels, respectively. The results showed that the sensitivity reached 4×101 copies/μL, and there was complete concordance between this method and direct DNA sequencing. Conclusion The new method is simple, rapid and accurate, which is suitable for genotyping SRBⅠ knockout mice. Related Articles | Metrics

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Effects of Tangshen Formula on liver oxidative stress and pathologic changes in typeⅡdiabetic rats LI Xin, GU Ligang, ZHAO Tingting, YANG Xin, ZHANG Yun, ZHANG Haojun, YAN Meihua, DONG Xi, ZHAO Hailing, WEN Yumin, LI Ping 2015, 43 (7):  735-738.  doi: 10.11958/j.issn.0253-9896.2015.07.009 Abstract ( 740 )   PDF (686KB) ( 4014 )   Objective To investigate the effects of Tangshen Formula on liver oxidative stress in diabetic rats, and their mechanisms thereof．Methods Rat model of type 2 diabetes mellitus and nonalcoholic fatty liver disease was established by intraperitoneally injecting small dose of chain urea with cephalosporins (STZ) and feeding high fat fodder．The model rats were randomly divided into control group, model group, Tangshen Formula group and metformin group．The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), cholesterol (CHO), low density lipoprotein-cholesterol(LDL-C), and superoxide dismutase (SOD), catalase(CAT), vitamin E(VE), nitric oxide (NO) and nitric oxide synthase (NOS) in liver were compared between four groups. Changes of pathological morphology were observed under light microscopy. Results There were significantly decrease in serum levels of ALT, AST, TG, CHO, LDL-C in Tangshen Formula group and metformin group compared with those of model group. There were significantly higher levels of SOD, CAT and VE, and lower levels of NO and NOS of liver homogenate in Tangshen Formula group than those of model group. There were higher levels of SOD and CAT, and lower levels of NO and NOS in liver homogenate in metformin group than those of model group. HE staining showed that liver fatty degeneration was significantly reduced in metformin group and Tangshen Formula group compared with that of model group. Conclusion The fatty liver in type 2 diabetic rats is significantly improved by Tangshen Formula treatment, which is probably associated with the regulation of the response level to oxidative stress in liver． Related Articles | Metrics

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NMDA receptors in central medial thalamus participate in propofol-induced unconsciousness ZHANG Yi, LUO Zhuxin, WANG Yuan, DUAN Zikun, LI Jia 2015, 43 (7):  739-741.  doi: 10.11958/j.issn.0253-9896.2015.07.010 Abstract ( 826 )   PDF (535KB) ( 4175 )   Objective To investigate the role of NMDA receptors in central medial thalamus (CMT) in the unconsciousness induced by general anesthesia. Methods A total of 60 rat models for microinfusion were assigned into 4 groups (n=15 for each group). After induction with propofol, 10 mmol/L (NMDA10 group), 20 mmol/L (NMDA 20 group) and 40 mmol/L (NMDA40 group) of NMDA and normal saline (group C) with equal volume were microinfused into CMT. The incidence of purposeful movement and recovery time of righting reflex were observed in each group respectively. Infusion sites were localized by histological method. Results When the microinfusion site localized within CMT, comparing with group C, the recovery time of righting reflex reduced notably in three NMDA groups (P < 0.05). The recovery time was significantly shorter in NMDA20 group and NMDA40 group than that of NMDA10 group. The incidence of purposeful movement during propofol anesthesia was higher in NMDA20 group and NMDA40 group than that of group C (P < 0.05). When the microinfusion site localized out of CMT, the recovery time of righting reflex was remarkably longer than that within CMT in three NMDA groups (P < 0.05), and there was no significant difference in the incidence of purposeful movement and recovery time between four group (P＞0.05). Conclusion Microinfusion of NMDA agonist into CMT reverses propofol anesthesia, indicating that NMDA receptor in CMT may contribute to the propofol-induced unconsciousness. Related Articles | Metrics

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Study of hydrodynamic in vitro and animal experiment of a homebred pneumatic pulsatile ventricular assist device SHI Guoning, CHEN Qingliang, CHEN Tongyun, LIU Jianshi 2015, 43 (7):  742-744.  doi: 10.11958/j.issn.0253-9896.2015.07.011 Abstract ( 851 )   PDF (819KB) ( 3602 )   Objective To study in vitro hydrodynamics of a pneumatic pulsatile ventricular assist device developed exclusively by China, and establish an animal model for the detection by the device. Methods The hydromechanics experiment was performed on an in vitro test loop using MEDOS-System to drive the ventricular assist device, and lycerl-water solution was used as circulating medium. The changes of afterload pressure and the output of the pump were monitored, and the impermeability and stability were also assessed after the experiment. Six adult dogs were used as the experimental animals. The device worked in the left heart assistance mode for 1 hour then the ventricular fibrillation was induced by potassium chloride, and then defibrillated after 5 min while the device remained working. The hemodynamics data were monitored consecutively during the trial. Results The ventricular assist device worked stably and reliably during the hemodynamic experiment. The pump can generate more than 4 L/min flow against the afterload pressure of 100 mmHg. There were no significant changes in heart rate at different time points in experimental dogs after left ventricular assist. Comparison between after auxiliary immediately and former auxiliary, the diastolic blood pressure of dogs increased 30 mmHg with the ventricular assist, and the diastolic pressure increased 19 mmHg. No obvious fluctuation in blood pressure was found during the auxiliary process. The diastolic blood pressure stayed at 60 mmHg when the heart was in ventricular fibrillation, and returned to normal after electrical defibrillation. Conclusion The ventricular assist device works stably in vitro test, and the pump can meet the need of adult’ s ventricular assist. It is effective and security to dogs in short term. The effects of long-term use need to be future proved. Related Articles | Metrics

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The influence of PI-88 on heparanase protein expression of human esophageal cancer cell and xenograft of nude mice ZHU Hui, HE Ming, CHEN Xin, LI Baozhong, XU Xinjian, LI Fei 2015, 43 (7):  745-748.  doi: 10.11958/j.issn.0253-9896.2015.07.012 Abstract ( 777 )   PDF (568KB) ( 3651 )   Objective To explore the inhibitory effects of sulfated oligosaccharides PI- 88 on the heparanase protein expression of human esophageal squamous cancer cell (ESCC) line TE-13, and to explore the effects of growth, angiogenesis and heparanase protein expression on ESCC xenografts of nude mice. Methods TE-13 cells were cultured and divided into three groups: group A (control group), group B (15 mg/L PI-88) and group C (30 mg/L PI-88). Heparanase protein expression of TE- 13 cells was measured by Western blot assay after being cultured for 36 h. The ESCC suspension was injected subcutaneously in 10 BALB/c/nu mice to build up ESCC xenograft model. The model mice were divided randomly into observation group and control group (5 mice per group). The mice in observation group received 40 mg/(kg·d) PI-88. The mice in control group only received the same volume of saline at the same time. Both PI-88 and saline were daily administrated for 14 days. Every 2 days, the volume of xeongrafts were measured and the mice were executed at the 14th day. CD34 immunohistochemical staining was used to detect the micro vessel density (MVD) of xenografts. Western blot assay and immunohistochemical staining were used to detect the heparanase protein expression of xenografts. Results The heparanase protein expressions of TE-13 cells were significantly decreased in group B and group C than those of group A (P < 0.001), with a kind of PI-88 dose-dependent manner. The volume, MVD and heparanase protein expression of xenografts were significantly lower in observation group than those of control group (P < 0.05). Conclusion The heparanase protein expression in TE-13 cells can be inhibited by PI-88 in vitro and vivo. Furthermore, the growth and angiogenesis of ESCC xenografts were also inhibited by PI-88. Related Articles | Metrics

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Experimental study on repairing spinal cord injury by human umbilical cord blood CD34+ cell transplantation at different time points TANG Liang, FENG Shiqing, GAO Ruixiao 2015, 43 (7):  749-752.  doi: 10.11958/j.issn.0253-9896.2015.07.013 Abstract ( 842 )   PDF (1160KB) ( 3726 )   Objective To investigate the effect of transplantation of human umbilical cord blood CD34+ cells on spinal cord injury. Methods CD34+ cells were separated from fresh human umbilical cord blood by magnetic cell sorting. Ninetysix female Wistar rats were injured at T10 by IMPACTOR MODEL-Ⅱ, and then randomly assigned to three groups: Cyclosporin A (CsA)+Dexamethasone (Dex) treated group (Ⅰ, n=32), local transplantation of cells+CsA+Dex treated group (Ⅱ) at the first day after operation (DAO 1, n=32), local transplantation of cells+ CsA+Dex treated group ( Ⅲ) at DAO 6 (n=32). BBB locomotor scoring system was used to assess the recovery of the lower limbs. The survival and neural differentiation of transplanted cells at the injury site were observed by double immunofluorescence. The tissue vitality at the injury site was observed by 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) staining, and the blood vessel density was observed by infusing mixture of Chinese ink and glutin followed by HE staining. Results BBB score at DAO 8-56 was significantly higher in Ⅱ group than that of other two groups (P < 0.05). TTC staining showed that the proportion of decreased vitality area was significantly smaller inⅡ group than that of other two groups (P < 0.01). The result of gelatin ink perfusion showed that the blood vessel density at the injury site was significantly bigger in Ⅱ group than that of other two groups (P < 0.01). There were more survival transplanted cells inⅡ group than those of III group (per visual field, 7.51±1.00 vs 5.51±0.89， t=6.051, P < 0.01). All the transplanted cells didn’ t differentiate into neural cells. Conclusion Human umbilical cord blood CD34+ cells can promote the recovery of the lower limbs after spinal cord injury by repairing blood vessels to increase tissue vitality at the injury site in rats. Related Articles | Metrics

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Comparison of pulmonary function between chronic obstructive pulmonary disease and overlap syndrome in patients LI Qun, WANG Yan, CAO Jie, ZHOU Ning, WANG Jie, GUO Meinan 2015, 43 (7):  753-757.  doi: 10.11958/j.issn.0253-9896.2015.07.014 Abstract ( 894 )   PDF (336KB) ( 3800 )   Abstract: Objective To explore the difference in pulmonary function between overlap syndrome and chronic obstructive pulmonary disease (COPD) in patients. Methods One-hundred-ninty-four patients came from respiratory department in Tianjin Medical University General Hospital, from October 2012 to August 2014, were included in this study. After inquisition of medical history, evaluation of lung function and polysomnography (PSG), patients were divided into overlap syndrome (OS, n=78) group, COPD group (n=76) and healthy control group (n=40). The indexes of lung function and the pulse oscillation index of pulmonary function were compared between three groups. Results Values of the peak expiratory flow rate (PEF) and the remaining 75% FVC when expiratory flow rate (MEF75) were significantly decreased in OS group than those of COPD group (P＜ 0.05). Patients with sleep apnea hypopnea index (AHI) ≥30 times/h showed significantly decreased MEF25 and the maximum mid expiratory flow (MMEF) in OS group than those of COPD group (P＜ 0.05). Values of the total impedance (Zrs), the resonant frequency (Fres), viscosity resistance when oscillation frequency was 5 Hz (R5), R20 were significantly increased in OS group than those of COPD group (P＜ 0.05). Patients with AHI ≥30 times/h were increased significantly than COPD group in R5-R20 and X5 besides Zrs, Fres, R5, R20. Conclusion There is more serious airway obstruction in patients with OS than that in patients with COPD. Severe sleep disordered breathing can aggravate small airway obstruction in patients with COPD Related Articles | Metrics

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The role of RET proto-oncogene methylation in the pathogenesis of Hirschsprung's disease ZHANG Shujian, ZHANG Hui, ZHAN Jianghua 2015, 43 (7):  756-760.  doi: 10.11958/j.issn.0253-9896.2015.07.015 Abstract ( 837 )   PDF (765KB) ( 3684 )   Abstract: Objective To investigate the relationship between the methylation of RET (proto-oncogene, RET) and Hirschsprung disease (HD), and understand its significance in the development of intestinal wall ganglion cells. Methods Twenty-one surgical removal specimens, which were all dilation segment of HD in Tianjin Children’ s Hospital, were used as experimental group, and 5 samples of non-HD normal colon tissues were used as control group. The bisulfite sequencing (BSP) - direct detecting method was used to detect RET CpG island methylation status. The expression of RET protein was Related Articles | Metrics

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Significance of RhoA and Snail expression in salivary adenoid cystic carcinoma HU Ruili, AN Feng, LIN Yuanyuan, MA Sai, GUO Bowei 2015, 43 (7):  759-763.  doi: 10.11958/j.issn.0253-9896.2015.07.016 Abstract ( 797 )   PDF (733KB) ( 3660 )   Abstract: Objective To investigate the relationship of RhoA and Snail expressions, and the invasion and metastasis in salivary adenoid cystic carcinoma (SACC). Methods The expressions of RhoA protein and Snail protein in 55 samples of SACC (SACC group ) and 20 samples of para-carcinoma normal tissues(control group) were detected using immunohisto⁃ chemical method. The relationship between RhoA protein and Snail protein expressions and clinical and pathological charac⁃ teristics were analyzed. Results The positive expressions of RhoA protein (69.1% vs 5.0%) and Snail protein (72.7% vs 10.0%) were significantly higher in SACC group than those in control group (P < 0.05). The positive expression rates of RhoA protein and Snail protein were significantly higher in patients with lymph node metastasis than those in patients with⁃ out lymph node metastasis. The positive expression rates of RhoA protein and Snail protein were significantly higher in pa⁃ tients at Ⅲ +Ⅳ stage than those in patients at Ⅰ +Ⅱ stage. The positive expression rates of RhoA protein and Snail protein were significantly higher in substantive carcinal tissues than those in screen roller type and tubular carcinal tissues. The posi⁃ tive expression of Snail protein was significantly higher in substantive and tubular carcinal tissues than that in screen roller type carcinal tissues (P < 0.05). There were no significant differences in positive expression rates of RhoA and Snail between different gender, age and different carcinal tissues. There was a positive correlation beween expression rates of RhoA and Snail protein in SACC (r=0.414, P < 0.001). Conclusion RhoA and Snail may both facilitate the infiltration and metastasis of SACC through RhoA/ROCK/PKD1/NF-kappa B/Snail signaling pathways References | Related Articles | Metrics

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The Associations of Serum 25OHD Levels with Semen Parameters JIA Xinzhuan, WEI Lan 2015, 43 (7):  762-765.  doi: 10.11958/j.issn.0253-9896.2015.07.017 Abstract ( 747 )   PDF (266KB) ( 4212 )   Abstract: Objective To analyze the relationship between serum 25 hydroxy vitamin D (25OHD) levels and semen pa⁃ rameters. Methods The serum levels of 25OHD were examined in 68 healthy men, who were divided into three groups. Twenty-two cases were included in 25OHD normal group (30 μg/L≤25OHD<40 μg/L), 20 cases were in 25OHD relatively insufficient group (20 μg/L≤25OHD<30 μg/L), and 26 cases were in 25OHD deficiency group (25OHD<20 μg/L). The se⁃ rum reproductive hormones including follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E 2), prolactin (PRL) and testosterone (T) were detected by chemiluminescence immune analysis. Semen samples were collected by mastur⁃ bation after a period of abstinence for 3 and 7 days. The total sperm count, sperm concentration, percentage of progressive motile sperm and percentage of normal morphology sperm were measured by sperm quality analyzer. Results There were no significant differences in FSH, LH, E2, PRL and T levels between three groups, and which were all in normal ranges. The total sperm count, sperm concentration, percentage of progressive motile sperm and percentage of normal morphology sperm were significantly lower in 25OHD relatively insufficient group and 25OHD deficiency group than those in 25OHD normal group. The total sperm count and the percentage of progressive motile sperm were significantly lower in 25OHD deficiency group than those in 25OHD relatively insufficient group (P < 0.05). There were no statistical differences in sperm concentra⁃ tion and percentage of normal morphology sperm between 25OHD deficiency group and 25OHD relatively insufficient group. Conclusion The decrease of semen quality may be related to serum 25OHD at the relatively insufficient and deficiency lev⁃ els. The effect of 25OHD on semen parameters may not be mediated by reproductive hormones. Related Articles | Metrics

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Relationship of body mass index and blood lipid level with cancer detection on prostate biopsy ZHANG Haoyu, LYU Guanglin, YUAN Hexing, WEI Xuedong, HU Linkun, ZHANG Xuefeng, HOU Jianquan 2015, 43 (7):  765-768.  doi: 10.11958/j.issn.0253-9896.2015.07.018 Abstract ( 787 )   PDF (274KB) ( 5168 )   Abstract: Objective To investigate the relationship between body mass index (BMI), blood lipid level and cancer dectection on prostate biopsy. Methods A total of 214 patients undergoing a prostate biopsy during 2013.2—2014.8 were reviewed retrospectively. They were divided into prostate cancer and non-cancer groups by biopsy results. The differences of age, prostate-specific antigen (PSA) level, prostate volume (PV), blood lipid level and BMI between two gtoups were analyzed. Risk factors for cancer dectection on biopsy were also analyzed. Results Compared with non-cancer patients, prostate cancer patients were older, had higher PSA level and BMI, but smaller PV and lower HDL-c level (P<0.05). Logistic regression analysis showed that older, higher PSA level and BMI were risk factors for cancer dectection on biopsy, but larger PV and higher HDL-c level were protective factors (P<0.05). Conclusion Comprehensive assessment of patients’ BMI and blood lipid levels can provide important reference for prostate cancer screening at early time and establishment of prostate biopsy scheme, which also provide significant evidence for the prevention, diagnosis and treatment of prostate cancer at early stage for high risk population. Related Articles | Metrics

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Study on aminoglycoside antibiotics resistance of Pseudomonas aeruginosa isolated fromdiabetic foot infections WU Hongfang, SUN Qian, LI Yuzhu, ZHANG Min, MENG Lingling, LI Daiqing 2015, 43 (7):  768-773.  doi: 10.11958/j.issn.0253-9896.2015.07.019 Abstract ( 834 )   PDF (651KB) ( 3868 )   Abstract: Objective To investigate the clinical features, phenotypes and genotypes of Pseudomonas aeruginosa (PA) strains isolated from patients with diabetic foot infection (DFI) resisting to aminoglycosides antibiotics (AmAn). Methods The clinical profiles of 209 DFI patients hospitalized in the Tianjin Metabolic Diseases Hospital were collected and ana⁃ lyzed. Forty-one PA strains were identified, and their antibiotic resistance profiles were obtained. The DNAs of PA isolates were extracted and applied to amplifications for several aminoglycosides modifying enzyme genes, including aac(3′)-Ⅰ , aac (3′)-Ⅱ , aac(6′)-Ⅰ b, aac(6′)-Ⅱ , ant(2′′)-Ⅰ and ant(3′′)-Ⅰ by PCR method. Combining with the clinical features and the antibiotic resistance profiles, the relationship between genotypes and phenotypes of the PA strains was analyzed. Results Gram positive bacteria (G+) were the majority of the pathogen with 51.67% detection rate. The total detection rate of PA was 19.62%, listed as the top one pathogenic bacterium among gram negative bacteria (47.67%). There was significant difference in the ratio of ulcer area≥4 cm2 between PA group and non-PA group and G + group. There were significantly higher inci⁃ dence rate of ischemic ulcer and osteomyelitis in PA group than those of G + group. There were higher clinical characteristics and ulcer depth (SAD) score, and increased hypersensitive C-reactive protein in PA group than those of G + group. There were 30 strains of PA being resistant to AmAn (73.17%). The predominant drug resistance gene to AmAn was ant(3′′)-Ⅰ (65.85%), and aac(3′)-Ⅰ gene was not found from all PA isolates. Conclusion The detection rate of PA isolated from DFI patients was higher, and patients were with the characteristics of larger, deeper and severe ischemia of ulcer area. The phe⁃ nomenon of PA resistant to AmAn was more serious, and ant(3′′)-Ⅰ gene identified from PA isolates was the most common resistance gene identified to AmAn Related Articles | Metrics

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In vitro efficacy of the combination of Cinnamaldehyde and Voriconazole on Aspergillusfumigatus isolates LUO Jin, CHEN Yiqiang, KONG Jinliang, HUANG Hong, HOU Changchun, LI Bing, DONG Biying 2015, 43 (7):  773-777.  doi: 10.11958/j.issn.0253-9896.2015.07.020 Abstract ( 761 )   PDF (391KB) ( 3690 )   Abstract： Objective To evaluate the in vitro antifungal activity of Cinnamaldehyde in combination with Voriconazole (VRC) against clinically isolated Aspergillus fumigatus strains. Methods According to the Clinical and Laboratory Stan⁃ dards Institute (CLSI) M38-A2 document， the minimal inhibitory concentrations (MIC) of Cinnamaldehyde and VRC alone or in combination against 42 clinical Aspergillus fumigatus isolates were determined by both microdillution method and check⁃ board method respectively. The MIC50, MIC90, MICG and MICs distribution were compared between single drug and both in combination. The concentration-accumulative curve was drawn and fractional inhibitory concentration index (FICI) was cal⁃ culated to evaluate the interaction between two test agents. Results The values of MIC50, MIC90 and MICG were significant⁃ ly decreased (P＜ 0.001) when combination of the two drugs than those of their single use, with their MIC distribution concen⁃ tration-accumulative curves shifted to the left. The value of FICI of Cinnamaldehye-VRC combination ranged from 0.187 5 to 1.5. Sixteen strains (38.10%) of them showed the synergistic effect, 19 strains (45.23%) showed additive effect, and 7 strains (16.67%) showed an unrelated effect, and no antagonist effect on tested Aspergillus fumigatus strains in vitro. Conclu⁃ sion Cinnamaldehye in combination with VRC mainly shows a combined synergic and additive inhibitory effect on Asper⁃ gillus fumigatus isolates, and this combination appears to be more active against the test strains, which are less susceptible to voriconazole. Related Articles | Metrics

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Diagnostic value of random spot urine albumin/creatinine ratio in women with hypertensivedisorders complicating pregnancy YIN Hong, ZHONG Mei 2015, 43 (7):  777-781.  doi: 10.11958/j.issn.0253-9896.2015.07.021 Abstract ( 996 )   PDF (348KB) ( 4147 )   Objective Determined random spot albuminuria to creatinine ratio(ACR) ，24 hours urinary protein and other biochemical indicators. Other biochemical indicators, analyzed the correlation of them, to discuss the diagnostic value of ACR in women with hypertensive disorders complicating pregnancy. Methods 584 women with hypertensive disorders complicating pregnancy including 169 gestational hypertension, 205 mild preeclampsia, 173 severe preeclampsia, 37 chronic hypertension complicating pregnancy and 2038 normal pregnant women were collected over the same time. ACR, 24 hours urinary protein, routine examination of blood and urine, coagulative function, liver and kidney function, fasting plasma glucose, glycated hemoglobin, insulin, C-Peptide, lipids were determined and compared in all the cases. Bivariate correlation analysis was used to analyze the relationship of ACR, 24 hours urinary protein quantitative and other biochemical indicators. To identify the independent risk factor of hypertensive disorders complicating pregnancy on logistic regression analysis. Receiver operating characteristic (ROC) curve was used to analyze the sensitivity, specificity and optimal cut off value of ACR for predicting hypertensive disorders complicating pregnancy. Results （1）ACR, 24 hours urinary protein quantitative, blood urea nitrogen, serum creatinine, uric acid, total cholesterol, low density lipoprotein cholesterol (BUN, Cr, UA, TC, LDL) were significantly higher in women with severe preeclampsia, however, platelet (PLT), serum albumin, total protein were significantly lower. In women with mild preeclampsia group, ACR, 24 hours urinary protein quantitative, UA was significantly higher, while serum albumin significantly lower than that in the other two groups.（2）There were positive correlation between the ACR and 24 hours urinary protein quantitative, urea nitrogen, serum creatinine, UA, D-dimer, TC, LDL (r=0.94, 0.37, 0.35, 0.61, 0.12, 0.33, 0.31, p＜0.05 ), while negative correlation between ACR and serum albumin, total protein, PLT (r=-0.46, -0.40, -0.12, p＜0.05 ) （3）Area under curve (AUC), sensitivity, specificity and optimal cut off value of ACR for predicting gestational hypertension, mild preeclampsia, severe preeclampsia ( 24 hours urinary protein quantitative≥2g) severe preeclampsia ( 24 hours urinary protein quantitative≥5g) were 0.823, 0.89, 0.61, 1.44mg/mmol, 0.988, 0.97, 0.96, 10.48 mg/mmol, 0.978, 0.97, 0.88, 39.84 mg/mmol, 0.973, 0.95, 0.87, 94.91 mg/mmol separately. Conclusions There was positive correlation between ACR and 24 hours urinary protein quantitative. ACR could be used for diagnosing and estimating the severity degree of hypertensive disorders complicating pregnancy. Related Articles | Metrics

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The relationship between total IgE and atopic dermatitis in maternal serum and neonatal umbilical cord blood and allergen testing ZENG Sanwu, MENG Linghe, WANG Deling, ZOU Yingxue, TANG Naijun, CHEN Xu, WEI Wenguo 2015, 43 (7):  781-784.  doi: 10.11958/j.issn.0253-9896.2015.07.022 Abstract ( 740 )   PDF (348KB) ( 4154 )   Abstract： Objective To explore the correlation of maternal serum and newborn cord blood total IgE and childhood atopic dermatitis (AD), as well as its clinical significance of allergen testing. Methods Selected 35 cases which were diagnosed as AD(AD group), and randomly selected 35 cases of the baby who were not diagnosed as AD (control group) from a birth cohort established in 2009—2011.Detected maternal serum and newborn cord blood total IgE level by the ELISA method. Used quantitate immunoblotting method to detect serum specific IgE antibody level. Results The serum total IgE level of the mothers and cord blood in the AD group are significantly higher than the control group (χ2=16.568 and 14.933，P < 0.01). Compared to the control group, there was statistical significance of dust mites, house dust, ragweed pollen, song kind of pollen, poplar, surname and elm pollen, mould, shrimp, marine fish of mother serum allergen in AD group（P < 0.05）; there was statistical significance of song kind of pollen, mould of newborn cord blood in AD group（P < 0.05）. Conclusion Advanced mother serum total IgE may play a predictive effect on infants suffering` from AD.There is no obvious consistency in allergic state between mothers and infants. Related Articles | Metrics

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The study on dynamic changes of plasma D-dimer in elderly patients with acute exacerbation of COPD combined with type 2 diabetes mellitus WANG Yanchun, ZHANG Junfeng, WEI Dianjun, LIU Chunli 2015, 43 (7):  784-792.  doi: 10.11958/j.issn.0253-9896.2015.07.023 Abstract ( 951 )   PDF (403KB) ( 4001 )   Abstract: Objective to investigate the dynamic changes of the levels of plasma D-dimer, C-reactive protein (CRP) and fasting plasma glucose (FBG) in elderly patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) combined with type 2 diabetes mellitus. Methods AECOPD group(n=65) as A group , AECOPD combined with type 2 diabetes mellitus group(n=65) as B group, The levels of D-dimer, CRP and FBG were observed and compared in the first day, third day, seventh day and the 14th day after hospital admission in two groups, and the correlation analysis between D-dimer, CRP and FBG. Logistic regression analysis of the factors that increased plasma levels of D-dimer. Results The levels of D-dimer, CRP and FBG of B group were higher than A group in the first day, third day and the seventh day after hospital admission（P<0.01）, D-dimer and CRP in A group declined obviously in the seventh day after hospital admission, but in B group declined obviously until the 14th day. In A group there was no significant difference on FBG, In B group FBG significantly decreased. there was a positive correlation between CRP,D-dimer and FBG（P<0.05）.Logistic regression analysis showed that CRP, FBG, p（O2）and age were the influence factors for the increased levels of D-dimer . Conclusion in elderly patients with AECOPD combined with type 2 diabetes mellitus, we should pay attention to anti-inflammatory and controlling blood sugar, at the same time dynamic monitoring for the levels of D-dimer, that help to improve the clinical and prognosis for patients with COPD, and reduce mortality. Related Articles | Metrics

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A long-term follow-up study on the treatment of small cell lung cancer using teniposide/ carboplatin with etoposide/carboplatin as first-line therapy CHEN Qingqing, ZHANG Huilai, WANG Huaqing 2015, 43 (7):  788-792.  doi: 10.11958/j.issn.0253-9896.2015.07.024 Abstract ( 958 )   PDF (363KB) ( 4104 )   Objective To compare the efficacy and toxicity of teniposide plus carboplatin（TC） and etoposide plus carboplatin（EC） in treatment of newly diagnosed SCLC，and the possible role of VM-26 on prevention of brain metastasis of SCLC. Methods A total of 102 previously untreated SCLC patients without brain metastasis were included； 64 patients received EC（VP-16 group） and 38 patients received TC（VM-26 group）. The characteristics of patients were comparable according to χ2 test. One month after chemotherapy all patient get CR or PR began prophylactic cranial irradiation（PCI）. Results The overall response rate was 78.9% for VM-26 group with 4 complete response(CR) and 26 partial response(PR)，and 76.6% in VP-16 group with 7 CR and 42 PR(P=0.826). Median PFS was 10 months（95%CI 7.4～12.6） in VM-26 group and 9 months（95%CI 6.4～11.6）in VP-16 group(P=0.866).Median survival was 18 months（95%CI 16.5～19.5） and 16 months（95%CI 9.9～22.1）in VM-26 group and VP-16 group(P=0.642), reapectively. The 1，2 and 3-year survival rates were 73.7%、36.8% and 18.4% in VM-26 group; and 71.9%、37.5% and 18.8% in VP-16 group(P﹥0.05). The rate of brain metastasis was 21.1% for VM-26 group and 43.8% for VP-16 group(P=0.020). The main toxicity was grade 1/2 mylosuppression，and there was no significant difference between the two groups（P﹥0.05）. Conclusion TC is a highly active regimen for treatment of SCLC；there is no difference in the effectiveness and toxicity versus EC. Application of VM-26 can reduce the incidence of brain metastasis in SCLC patients. Related Articles | Metrics

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The application of acridine orange fluorescent staining method in circulating tumor cell LIU Min, MA Fuling, HAN Rong, LI Mei, LIU Shufen, ZHANG Shumin 2015, 43 (7):  792-796.  doi: 10.11958/j.issn.0253-9896.2015.07.025 Abstract ( 783 )   PDF (389KB) ( 3882 )   Objective ：To explore the screening method of circulating tumor cells (CTCs) staining with acridine orange fluorescent (AO-F) and its application in metastatic renal cell carcinoma (RCC) patients. Methods: Culture 769-P cell line and primary RCC cells，calculate the percentage of AO-F positive staining 769-P cell under 5 random sights after AO-F staining and evaluate the sensitivity of AO-F to cells. 5ml morning fasting venous blood of all controls was obtained to enrich nucleated cells. The CTCs model of renal cancer was established when known numbers of tumor cell（500、200、100、50、10）respectively were mixed with 106 nucleated cells which were used to evaluate A0-F method’s specificity and reproducibility. 27 metastatic RCC patients were collected and use AO-F staining method to detect the expression of AO-F positive cells in their blood. Analyse the correlation between expression and clinical parameters. Results: The percentage of AO-F positive staining 769-P cell was 93%±3% under 5 random sights. The recovery rate(%) of four groups (500、200、100、50) was 10.2±3.8、9.2±2.3、10.8±2.6、10.5±1.9, respectively. No significant difference was found among four groups（F=1.001，P＞0.05）.The group（10）could find AO-F positive staining cells occasionally. Zero case in controls was positive. 9 cases of 27 in test group were positive and the rate was 33.33%. There were no significant statistical difference between gender, ages, tumor sizes, pathological pattern, Furhman stages, metastasis of lung or not, presence of tumor or not and AO-F positive rate (P >0.05). Conclusion: It is confirmed that the method of CTCs staining with AO-F, which has high specificity and reproducibility, is feasible to detect CTC and worthy of being studied. There is a certain reference value to predict tumor recurrence and metastasis. Related Articles | Metrics

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Knowledge, attitudes and practices of post abortion family planning services in Tianjin YANG Jing, HUO Yan, ZHANG Yujuan, WANG Jianmei, WANG Zhenxia 2015, 43 (7):  796-799.  doi: 10.11958/j.issn.0253-9896.2015.07.026 Abstract ( 717 )   PDF (368KB) ( 4208 )   　　　Objective To better understand the knowledge, attitudes, practice(KAP) status and relative demand to post abortion family planning service(PAFPS) in medical staffs of obstetrics and gynecology department in Tianjin. Methods By using random, stratified and clustered sampling approach, we selected 290 obstetrics and gynecology personnels in Tianjin. A Self-Administered Questionnaire on PAFPS KAP was employed to survey the general characteristics, awareness of PAFPS knowledge, attitudes to PAFPS, the occurrence of unintended pregnancy themselves, service capabilities of PAFPS and request of continuing education. Results The respondents had defects in PAFPS forms, follow-up arrangements and contraceptive method after abortion. Doctors’ average knowledge score was higher than nurses’. The higher the level of medical institutions and job titles, the higher the knowledge score. 95.34% of respondents believe that it is necessary to develop PAFPS widely. But 93.55% of respondents think it is difficult to carry out PAFPS as required because of unclear service process, shortage of funds, human resource constraints, inadequate facilities and weak sense of service. 53.36% of respondents who had sexual life history occurred unintended pregnancy themselves. The current defects of PAFPS were hadn’t standardized service processes, missing of medium-term and long-term follow-up, missing of collective consulting, providing of contraceptives not timely and hadn’t pay attention to contraceptive advice. 90.68% medical personnel showed demand on relative training. Conclusion It’s necessary to perform continuing education about PAFPS to obstetrics and gynecology personnels via different intervention. At the same time, we should increase investment and make the service processes perfectly, so as to improve the level of PAFPS among obstetrics and gynecology medical staffs. Related Articles | Metrics

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The relative impact factors of postpartum pelvic floor muscle injury in the region of Tianjin GAO Wen, LYU Fengjun, ZHANG Cuiping, LI Weiqin, LI Baojuan 2015, 43 (7):  800-804.  doi: 10.11958/j.issn.0253-9896.2015.07.027 Abstract ( 607 )   PDF (456KB) ( 3814 )   Abstract： Objective To investigate the influences of different factors on postpartum pelvic floor muscle injury, and provide clinical evidence to the early prevention of pelvic floor dysfunction (PFD). Methods A total of 65 630 women, who participated the postpartum pelvic floor function screening in Tianjin, from December 2011 to December 2014 were selected in this study. The uniform detect methods was used in this study. The analysis based on the PFD was diagnosed by physi⁃ cians of Women’ s and Children’ s Health Center in each district. The influences of different factors on postpartum pelvic floor muscle were analyzed. Results There were 19 452 (29.6%) women suffering from postpartum pelvic floor muscle inju⁃ ry, with the abnormal rate 29.6%, which was higher in rural areas than that in urban areas (33.9% vs 27.8%). The abnormal rate was higher in women with junior middle education or below (34.1%) than that in women with senior middle or polytech⁃ nic schooling (30.4%), and women with college or higher education (27.7%). There was a higher abnormal rate in women with vaginal surgery delivery (40.8% ) than that in women with natural delivery (32.5% ) and women with caesarean section (27.2%). The muscle abnormal rate was higher in women having given two or more than two times of deliveries (34.6% ， 31.5%) than those having initial delivery (29.1%). Multiple factor analyses showed that city residency, higher education and less delivery times were protecting factors for postpartum pelvic floor muscle while vaginal surgery was a risk factor for post⁃ partum pelvic floor muscle. Conclusion There is a high incidence of postpartum pelvic floor muscle injury in the region of Tianjin. Analyzing the risk factors and strengthening the management of pelvic floor muscle injury by early prevention or ear⁃ ly treatment would help to reduce the incidence of PFD effectively during pre-pregnancy and perinatal periods. Related Articles | Metrics

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Analysis of serum lipid levels in elderly female cadre population in Tianjin ZHAO Xian, WANG Hui, TIAN Jianli, WANG Lin 2015, 43 (7):  804-807.  doi: 10.11958/j.issn.0253-9896.2015.07.028 Abstract ( 654 )   PDF (305KB) ( 4584 )   Abstract Objectives To find out the trend of blood lipid in female elderly with aging, we observed a elderly cadre population in Tianjin, and analyzed the general level, distribution features, and had further discussion the strategies of blood lipid regulation therapy and prevention and control of cardiovascular disease during senile period. Methods We analyzed the data of annual health check from the General Hospital of Tianjin Medical University Health Care Department of 2013 of blood lipid on female elderly in order to investigate the general level and abnormality features of blood lipid in this elderly female group. Results The average blood lipid level of this health screening groups of elderly female cadre in 2013 were total cholesterol (TC) 5.46±0.97 mmol/L, triglycerides (TG) 1.57±0.82 mmol/L, high density lipoprotein cholesterol (HDL-C) 1.59±0.44mmol/L, low density lipoprotein cholesterol (LDL-C) 3.18±0.89 mmol/L. The level of TC, TG and LDL-C increased with aging, and then decreased at the same age pointcut of 60-69, and was higher than the previous research in China of 45-49 age group. HDL-C level had a reverse pattern, decreased with aging and then increased at the same pointcut as above. Conclusions In 2013, this elderly female cadres population of health check was a well-educated, professional urban resident population. The level of TC, TG, LDL-C and HDL-C changed regularly with aging, and the age pointcut was higher than the previous research in China. The abnormality ratio of overweight, obesity and dyslipidemia were higher than previous research. The level of LDL/HDL, TG/HDL and non-HDL-C indicate the high risk of coronary heart desease. Related Articles | Metrics

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Screening for risk factors of stroke in people with family history of stroke DONG Wentao, ZHANG Zhen, JIN Zhangning, GAO Feng, ZHANG Litong, LI Fengtan, WANG Jinghua, YANG Xinyu 2015, 43 (7):  807-810.  doi: 10.11958/j.issn.0253-9896.2015.07.029 Abstract ( 683 )   PDF (303KB) ( 4150 )   Objective To investigate the characteristics of cerebral vascular imaging and the risk factors of stroke in population with family history of stroke in rural areas of Ji County, Tianjin. Methods Area volunteers with family history of stroke were recruited in the study area to undergo computed tomography and computed tomography angiography of the brain as well as physical and laboratory examinations. A total of 281 subjects underwent examination,153 were men and 128 were women. Results The smoking rate, drinking rate, educational level, and low HDL-C level detection rate were higher among men than among women, whereas the high TG level detection rate was higher among female subjects than among male subjects; all of these differences were statistically significant (p < 0.05).38 subjects belong to the stroke group, The age, internal carotid artery calcification rate(ICAC), and internal carotid artery tortuosity(ICAT) rate were significantly higher in the stroke group than in the non-stroke group (p < 0.05) . The results of multivariate logistic regression analysis indicated that Age, SBP, ICAC, and ICAT were independent risk factors for stroke. Conclusion For population with family history of stroke, age, hypertension, ICAC and ICAT were risk factors of stroke. Related Articles | Metrics

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Research strategy of the case-control post-genome-wide association study LIU Jiayu, ZHANG Li′na, ZHENG Hong 2015, 43 (7):  810-814.  doi: 10.11958/j.issn.0253-9896.2015.07.030 Abstract ( 814 )   PDF (333KB) ( 4082 )   Since the first paper reported the finding of genetic variation contributing to human age-related macular de⁃generation by genome-wide association study (GWAS) in 2005, large number of human complex diseases associated genetic variants have been identified through GWAS method. However, the biological function of these genetic variants is not very clear. The present paper reviewed the methods of fine-mapping and the progress of the functional studies for these suscepti⁃bility variants. In the post GWAS Era, the study of genetic mechanisms can help us to understand the disease pathogenesis. Related Articles | Metrics

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Research progress on the role of CaMKⅡ in cardiovascular disease WANG Pu, LIU Yangong, ZHENG Mingqi 2015, 43 (7):  813-818.  doi: 10.11958/j.issn.0253-9896.2015.07.031 Abstract ( 807 )   PDF (339KB) ( 4615 )   Calcium/Calmodulin-Dependent Protein KinaseⅡ (CaMKⅡ）has multiple function which made it play a central role in cardiovascular disease. Especially it activates numerous downstream targets in various signaling pathways that promotes vascular disease,heart failure, myocardial hypertrophy and arrhythmias. CaMKⅡ can impact calcium balance and increase calcium leak in myocardial cell via phosphorylating L type calcium channel, Ryanodine receptor (RyR2) and phospholamban (PLN), and regulate ATP sensitive potassium current (IKATP) and late sodium current by affect sodium channels and potassium channels . In addition, It will directly regulate transcription via activating the silk crack the original activated protein kinases (MAPKs) and acetylation enzyme (HDAC). These mechanisms has important roles in myocardial hypertrophy and heart failure, arrhythmia. So weWe focus to demonstrating the structure and action mechanism of CaMKⅡ to improve a new therapy of cardiovascular disease. Related Articles | Metrics

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The advances of genetic factors in spontaneous intracerebral hemorrhage and molecular mechanisms LIU Yang, LIU Xiu, CHEN Xuyi, SUN Shengkai, WANG Zhihong 2015, 43 (7):  819-823.  doi: 10.11958/j.issn.0253-9896.2015.07.032 Abstract ( 855 )   PDF (306KB) ( 4953 )   Spontaneous intracerebral hemorrhage (SICH) refers the primary, non-traumatic parenchymal hemorrhage.In China, SICH accounts for about 20%-30% of total strokes. SICH is a kind of disease affected by multiple factors includ⁃ing environmental and genetic factors. The high morbidity and mortality cause serious damage to human health. Therefore, it is important to find etiology and risk factors of SICH. The article reviewed the progress of SICH pathogenesis in the perspec⁃ tive of genetics and molecular biology. Related Articles | Metrics

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Research progress in strategy of preventing stone retropulsion during ureteroscopic lithotripsy LI Li, XU Yong, QI Shiyong 2015, 43 (7):  822-826.  doi: 10.11958/j.issn.0253-9896.2015.07.033 Abstract ( 738 )   PDF (323KB) ( 4312 )   Abstract: Stone retropulsion during ureteroscopic lithotripsy is a common troublesome problem for most urologists. Stone retropulsion can result in increase in health care cost and increased operative time. With the popularisation of ureteros⁃copy, additional procedures to treat residual migrated fragments has drawn more and more attention and research. In recent years, many equipment, devices and techniques are designed to resolve this big puzzle. We reviewed the literatures to sum⁃mary the strategy for preventing stone retropulsion during ureteroscopic lithotripsy. Related Articles | Metrics

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Research progress of miR-224 and tumor LIU Maosheng, ZHANG Jixiang 2015, 43 (7):  826-830.  doi: 10.11958/j.issn.0253-9896.2015.07.034 Abstract ( 815 )   PDF (319KB) ( 4867 )   MiR-224 is a small noncoding RNA that usually binds to response element of target mRNA through the seed sequences，leading to the suppression of translation or mRNA degradation。Recent researches indicate that miR-224 not only aberrantly expresses in multiple tumors （ hepatocellular carcinoma ，gastric carcinoma、diffuse large B—cell lymphoma，prostate cancer， colorectal cancer, etc），playing an important role in tumor suppressor or oncognen，but also has an effect on the chemosensitivity of tumor.In this article,we review recent studies about the mechanism of miR-224 in the tumor development ,progression and chemosensitivity. Related Articles | Metrics

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Research advances in IL-33 and its receptor ST2L in allergic rhinitis DU Yunyan, LUO Ying, WAN Jun, YANG Chunping, YANG Ying, HE Rongzhou 2015, 43 (7):  830-833.  doi: 10.11958/j.issn.0253-9896.2015.07.035 Abstract ( 818 )   PDF (301KB) ( 4467 )   Allergic rhinitis (AR) is a specific individual after contact with allergens of the performance of a noninfectious inflammatory response in the nasal mucosa.Th1/Th2 immune imbalance may play an important role in the pathogenesis of AR.Interleukin 33 (IL-33), the novel cytokine of IL-1 family,is an important regulatory factor in allergic diseases, autoimmune diseases and various inflammatory diseases.IL-33 as an alarm, mainly by the secretion and release of tissues and cells damaged, especially impaired epithelial cells and endothelial cells.IL-33 binding to its receptor ST2L can activate a variety of immune cells to produce Th2 cytokines, precipitating and maintaining Th2 polarization, increased AR immune inflammation, which is the new targeted of AR in research and treatment. In this article,we will do a brief overview for the biological functions of IL-33 and its receptor ST2L and the research progress in the AR. References | Related Articles | Metrics