Tianjin Medical Journal

›› 2014, Vol. 42 ›› Issue (12): 1202-1208.doi: 10.3969/j.issn.0253-9896.2014.12.015

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Relationship of plasma homocysteine with gene polymorphisms of MTHFR and MTRR among the Han women in Xiangtan city

WANG Shuyuan 1,, LU Yanqiang 2,MA Shaojie 2,HUANG Jingxi 1,YANG Kaiyun 1,XIONG Min 1,ZHOU Yuzhen 1,YANG Qi 3   

  1. 1. Xiangtan Maternal and Child Health Hospital,
    2. Shanghai Institute of Target Molecules
    3. NationalCenter for Women and Children’ s Health, Chinese Center for Disease Control and Prevention
  • Received:2014-03-27 Revised:2014-07-30 Published:2014-12-15 Online:2014-12-15
  • Contact: MA Shaojie E-mail:mashaojie@genechina.com

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Abstract:

[Abstract] Objective To investigate the relationship of plasma homocysteine with the genotype distribution of MTHFR and MTRR among Chinese Han women in Xiangtan. Methods MTHFR C677T, A1298C and MTRR A66G geno? typing was analyzed to detect the distribution of gene polymorphisms among 1 701 women from Xiangtan city then the data were compared with the rest of the Han women in Zibo, Zhengzhou, Yantai, Zhenjiang, Songzi, Huizhou, Qionghai. Plasma Hcy levels from 110 patients were measured and analyzed the correlation with gene polymorphisms. Results The frequency of MTHFR C677T genotype and allele frequencies in Xiangtan is 12.6% which is higher than Huizhou (10.9%) and Qionghai (6.1%) but lower than Zibo (43.6%), Zhengzhou (36.8%), Yantai (32.2%), Zhenjiang (21.8%) with statistically significant dif? ference (P < 0.05). There is no significant different in MTHFR C677T between Xiangtan and Songzi. The frequency of MTH? FR A1298C genotype and allele frequencies in Xiangtan is 4.8% which is lower than Qionghai（7.1%） but higher than Zibo （1.4%）， Zhengzhou（2.4%）, Yantai（1.8%）, Zhenjiang（3.5%） and Songzi（2.6%） with statistically significant difference. The frenquency of MTRR A66G genotype and allele frequencies in Xiangtan is 6.8% which is higher than Zibo (4.8%) but lower than Qionghai (9.3%) with statistically signifcant difference. Plasma Hcy concentration correlate with MTHFR C677T, Hcy concentration in TT population is higher than that in CT and CC population（μmol/L:8.52±2.01 vs 5.94±1.47 vs 5.71± 0.18）； Plasma Hcy concentration also correlate with MTHFR A1298C and Hcy concentration in CC population is higher than AA and AC population（μmol/L: 9.83±2.26 vs 6.35±2.13 vs 5.55±1.75）； Plasma Hcy concentration does not correlate with MTRR A66G. Conclusion The gene polymorphism of MTHFR C677T, A1298C and MTRR A66G among the Hanwomen in Xiangtan was statistically different from other selected regions of China. Mutation in MTHFR C677T and A1298C were associated with elevated plasma levels of Hcy.

Key words: Methylenetetrahydrofolate reductase, methionine synthase reductase, polymorphism, homocysteine