Abstract:

[Abstract]  Objective   To study the correlation between the expressions of Bcl-2and Caspase-3in intestinal epithelial and inflammatory cells of lamina propria and the staging and pathogenesis of ulcerative colitis (UC). Methods The  pathomorphological changes of 25 samples of UC in the active phase and remission phase and25samples of control were observed under microscope. The expressions of Bcl-2and Caspase-3were detected by immunohistochemistry SP method. The correlations between expressions of Bcl-2, Caspase-3and UC staging were analyzed. Results   There were no significant differences in expressions of Bcl-2and Caspase-3in intestinal epithelial cells between three groups (P>0.05). The expression of Bcl-2in inflammatory cells of lamina propria was significantly higher in active UC group than that of normal control group(P<0.05), no significant difference between UC remission group and normal control group (P>0.05). There was a higher positive expression of Caspase-3in inflammatory cells of lamina propria in UC remission group. There was correlation between Bcl-2, Caspase-3and UC staging (r=0.371and0.4453respectively). Conclusion   The expression levels of Bcl-2 and Caspase-3were related with pathogenesis of UC. The detection of expression levels of Bcl-2and Caspase-3has a certain application value in UC staging.

Key words: caspase3, bcl-2-associated X protein, Colitis, Ulcerative, epithelial cells, intestinal mucosa