Abstract: Abstract:Purpose To investigate the expression and clinical pathological significance of hCTR1 in the cervical carcinoma and cervical intraepithelial neoplasia. Methods Using tissue microarray and immunohistochemical staining to detect the expression of hCTR1 in 52 cases of cervical carcinoma, 94 cases of CIN and 23 cases of normal cervical squamous epithelia. Results ①The manual tissue microarray was satisfied in IHC staining,and it satisfied the requirement of our studying multiple samples,the detetectable rate was 89.4%.②The positive retas of hCTR1 in SCC(69.2%) and in the level CIN(CIN 2-3, 73.1%) were significantly higher than those of normal cervical squamous epithelium(17.4%) and CIN 1(29.6%) (P<0.05). Other groups are no significant different(P>0.05). ③No significant relationships were found between the expression of hCTR1 and clinical pathological features. Conclusions Using manual tissue microarray made all the cases under the same experiment standard, could minimize the experimental error. hCTR1 expression rates became higher and higher from normal cervical squamous epithelia, CIN to cervical squamous cell carcinoma.hCTR1 regulates the cisplatin concentration in tumor cells so that it may be used as a potential biomarker of cervical carcinoma.

Key words: hCTR1, cervical squamous cell carcinoma, cisplatin, tissue microarray techniques