Abstract:

[Abstract] Objective  To study the tumorigenicity of human mesenchymal stem cells from adipose tissue (hMSC-AT), and to provide safety data for its clinical application. Methods  Ninety-six nude mice were divided into blank control group， positive control group and adipose tissue-derived mesenchymal stem cell group (n=32 for each group). The hMSC-AT of the 5th passage was subcutaneously injected into nude mice. The mice were sacrificed 3 days, 1 month, 3 months and 6 months after injection respectively. The anatomical check was carried out to observe the tumor formation, and the ability to form colonies in hMSC-AT was observed by soft agar cloning method. Results  No abnormal cells were found by gross necropsy and histological examination in animals of hMSC-AT group and blank control group. In vitro test, hMSC-AT did not show the capacity for clonal growth in the medium. Conclusion  The hMSC-AT at low passage appears to be safe and is not tumorigenic.

Key words: Mesenchymal stem cells, Tumorigenicity, Nude mice, Soft agar cloning