Abstract: Objective   To study the antibacterial effect of wound dressing with microspheres containing levofloxacin in the live animal experiments and it’s cytotoxicity. Methods    According to ISO 10993-5:2009, study chitosan - cytotoxic effects of wound dressing with microspheres containing levofloxacin; vaseline gauze as a blank control, nano-silver trauma attached to experimental, the dressing as the experimental group, comparing different dressings after covering the wound, the number of colonies of the wound tissue, tumor necrosis factor (TNF-α) levels. Results    The vitro cytotoxic response of the dressing was 0/0. the number of colonies in 0.25,1,4,7 D were 96 ± 9 × 105CFU / g, 435 ± 235 × 105CFU / g, 5 ± 3 × 105CFU / g, 18 to 0; ± 9 × 105CFU / g; TNF-α levels in 0.25,1,4,7 D were 44.37 ± 3.39 Pg / mL, 41.45 ± 3.28 Pg / mL, 36.07 ± 3.63 Pg / mL, 32.91 ± 5.74 Pg / mL. Conclusion     the wound dressing was no significant cytotoxicity. with the growth of administration time, reduce the number of colonies and TNF-α, the wound dressing is a long-lasting antibacterial ability of medical dressings.

Key words: chitosan, ofloxacin, delayed-action, tumor necrosis factor-alpha, Ntibacterial agent, cytotoxicity tests