Abstract:

[Abstract]   Objective  To observe the effects of stromal cell-derived-factor-1(SDF-1) on the function of endothelial progenitor cells（EPCs）of peripheral blood in patients with diabetes, and to discuss the effects of PI3K/AKT signaling path?way on the role of SDF-1in EPCs. Methods   The peripheral blood samples (30mL) were collected in10diabetes patients (DM group) and 10healthy controls (HC group). (1) The 100μg/L SDF-1was added in intervention group. EGM-2MV was added in non-intervention group. The Boyden chamber and in vitro angiogenesis kit were used to analyze the migration and in vitro angiogenesis of EPCs. (2) Cultured EPCs were divided into blank control group, 1μg/L SDF-1group,10μg/L SDF-1group,100μg/L SDF-1group, pure AMD3100group and100μg/L SDF-1+AMD3100group. AKT protein expression lev?els of endothelial progenitor cells were detected by Western blot assay in each group.  Results   (1) Without intervention with SDF-1, EPCs’migration and angiogenesis ability were lower in DM group than those in HC group. After intervention with SDF-1, the migration and angiogenesis ability were enhanced in two groups, but the increased level was higher in DM group than that of HC group. (2) Under the same concentration, AKT protein expression level was significantly lower in DM group than that in HC group (P<0.01). AKT protein expression levels were increased with the increased levels of SDF-1in DM group and HC group (P<0.05). AKT protein expression was significantly lower in 100μg/L SDF-1+AMD3100group than that of 100μg/L SDF-1group (P<0.05). Conclusion   SDF-1can increase the chemotactic migration and angiogenesis ability of EPCs in peripheral blood, especially for patients with diabetes. The effects of SDF-1on EPCs were related to the PI3K/AKT signaling pathway.

Key words: Stromal cell-derived-factor-1, Endothelial progenitor cells, Phosphoinositide3-kinase /protein kinase B, Diabetes