Abstract:

[Abstract] Objective  To explore the diagnostic value of combined detection of the liquid array technology, interferon (IFN)-γ and IFN-γ-inducible protein (IP)-10 in the rapid, accurate diagnosis and differential diagnosis of tuberculous pleural effusions. Methods Patients with transudative pleural effusions were divided into tuberculous pleural effusion group (n=52) and malignant pleural effusion group (n=38). The method of T-SPOT.TB was used to detect the number of effee tor T cells sensitized to Mycobacterium tuberculosis and spot forming cells (SFCs). The liquid array technology was used to detect the level of IFN-γ and IP-10. Logistic regression was used to analyze and compare the diagnostic value of the twomethod combination. Results The diagnostic sensitivity, specificity and the area under the ROC curve (AUC) of T-SPOT. TB were 90.38%, 84.21%, and 0.938 (95%CI: 0.867-0.978), respectively. The diagnostic sensitivity, specificity and AUC of combined detection of IFN-γ and IP-10 were 98.08%, 97.37%, and 0.995 (95%CI: 0.951-1.000), respectively. There was no significant difference in the diagnostic sensitivity and specificity between the two methods, and the diagnostic agreement for the two diagnostic methods was fine (Kappa=0.703). The difference of AUC between the methods was significantly differ? ent (Z=1.996, P <0.05). The method of combined detection of IFN-γ and IP-10 showed the larger AUC (AUC= 0.995). Conclusion The combined diagnosis meets the clinical needs of rapid, accurate diagnosis and differential diagnosis for tuberculous pleural effusion by simultaneously assaying the level of IFN-γ and IP-10 using the liquid array technology.

Key words: interferon type Ⅱ, tuberculosis, liquid array technology, chemokine CXCL10, pleural effusion, malignant, ROC Curve, 结核感染T 细胞斑点试验