Tianjin Med J ›› 2015, Vol. 43 ›› Issue (12): 1382-1385.doi: 10.11958/j.issn.0253-9896.2015.12.010

• Experimental Study • Previous Articles     Next Articles

Protective effects of Trimetazidine on ischemia-reperfusion injury of fatty liver in rats

WANG Tengfei1,YANG Tao2△,ZHANG Wei2,FANG Zhenyu2,WANG Honghai2,LIU Lei2,ZHANG Haiming2, WANG Yuliang3   

  1. 1 First Central Clinical College of Tianjin Medical University,Tianjin 300192,China;2 Tianjin First Central Hospital;3 Key Laboratory for Critical Care Medicine of the Ministry of Health
  • Received:2015-08-03 Revised:2015-08-21 Published:2015-12-15 Online:2015-12-11
  • Contact: △Corresponding Author E-mail:yangtao5588 @medmail.com.cn E-mail:tengfeiwang6312@163.com

Abstract: Objective To investigate the protective effects of Trimetazidine(TMZ)on the ischemia reperfusion injury (IRI)of fatty liver in autotransplantation model. Methods Fatty liver model was established by feeding high fat diet. Male Wistar rats (n=30) were randomized into three groups; Sham group, TMZ group and Model group. Liver was autotransplanted in both TMZ group and Model group. Serum levels of ALT, SOD, MDA, Bcl-2 and activated Caspase-3 were assessed 6 hours after the operation. The pathological performances of liver were also determined. Results Compared with the Model group, serum levels of ALT,AST, MDA and SOD levels decreased significantly in the TMZ group(P < 0.05). Serum level of Bcl-2 was higher while level of activated Caspase-3 was lower in TMZ group than those in Model group(P<0.05). Histo⁃ logical assay and TUNEL staining showed reduced hepatocyte swelling and narrowed sinusoid as well as decreased hepatic apoptosis in TMZ group compared with Model group. Conclusion TMZ can reduce oxidative stress, promote the expression of Bcl-2 and inhibit the activation of Caspase-3, which all contribute to its protective effect on fatty liver with ischemia-re⁃ perfusion injury.

Key words: Trimetazidine, fatty liver, reperfusion injury, apoptosis, bcl-2-associated X protein, alanine transaminase, aspartate aminotransferases, malondialdehyde, superoxide dismutase, caspase-3