Abstract: Abstract： Objective To identify the differentially expressed genes and functional enrichment pathways using bioin⁃ formatics technology in mice who were acutely administered with Morphine. Methods First, we downloaded microarray da⁃ tasets of mice which were acutely administered with Morphine from Gene Expression Omnibus, and assess the quality control parameters of the microarray datasets using AffyQCReport 1.42.0 package by R programming language 3.1.0 software. Subse⁃ quently, the differentially expressed genes of the microarray datasets were identified using the linear models for microarray data of R language. Finally, functional pathways of the differentially expressed genes were enriched based on expression anal⁃ ysis and systematic explorer. Results The microarray datasets showed preferable uniformity and intension similarity. A to⁃ tal of 481 differentially expressed genes including Gm11627, Zfand4, Zbtb16, Pkp2 and Plin4 were identified. While 8 func⁃ tional enrichment pathways, including pathways in cancer, melanogenesis and mitogen-activated protein kinase signaling were revealed. Conclusion The differentially expressed genes and functional pathways were the underlying biomarkers of mice who were acutely administered with Morphine.

Key words: morphine, mice, microarray analysis, gene expression, biological markers； enrichment of functional analysis