Abstract: Abstract: Objective To detect the effects of exenatide on the related indicators of proliferation, invasion and apopto⁃ sis of cell line SCC-25. Methods SCC-25 cells were cultured in vitro. The expression level of glucagon like peptide 1 re⁃ ceptor (GLP-1R) was determined by Western blot assay in SCC-25 cells. SCC-25 cells were divided into four groups: con⁃ trol group and exenatide group (1, 10 and 100 nmol/L). The ability of cell proliferation was detected using MTT assay after 24 h, 48 h and 72 h of culture. The ability of invasion was measured with Transwell assays. The expression levels of MMP- 2, Caspase-3 and Phospho-p38 MAPK were measured by Western blot assay. Results GLP-1 receptor expression was found in SCC-25 cells. Compared with control group, the cell survival rate, invasion rate and the expression of MMP-2 were signifi⁃ cantly decreased in SCC-25 group (P < 0.05). The expression of Caspase-3 were significantly increased (P < 0.05).Changes were in a concentration-dependent and time-dependent manner (P < 0.05). The expression of Phospho-p38 MAPK was sig⁃ nificantly increased at 24 h in 10 nmol/L exenatide group (P < 0.05). Conclusion Exenatide can inhibit the cell prolifera⁃ tion and invasion, which may contribute the apoptosis by promoting expressions of Phospho-p38 MAPK and Caspase-3 of SCC-25 cells.

Key words: exenatide, tongue squamous cell carcinoma, GLP-1R, cell proliferation, neoplasm invasiveness, apoptosis