Table of Content

15 May 2015, Volume 43 Issue 5 Previous Issue    Next Issue

---

For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Select

Proteomic analysis of the serum from patients with acute rejection after liver transplantation#br# #br# JIANG Qi, RU Yawei, LI Keqiu, LI Guang 2015, 43 (5):  449-452.  doi: 10.11958/j.issn.0253-9896.2015.05.001 Abstract ( 748 )   PDF (572KB) ( 3869 )   Abstract: Objective To investigate the protein markers that specifically expressed in patients with acute rejection (ACR) after liver transplantation, and to explore preliminarily the mechanisms. Methods Serum samples from three pa⁃ tients with pathologically confirmed ACR after liver transplantation in Tianjin First Central Hospital were collected as ACR group. Three serum samples from patients with normal liver function indicators after liver transplantation were collected as No-ACR group. And six serum samples from healthy examination were mixed with equal amount as healthy control group. Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) was employed to separate, screen and identify the differential⁃ ly expressed proteins between three groups. KEGG and STRING software were applied to deeply analyze the data of three groups. Results A total of 88 differentially expressed proteins were found between ACR group and healthy control group. There were 39 differentially expressed proteins between No- ACR group and healthy control group. Ten differentially ex⁃ pressed proteins were acquired between ACR group and No-ACR group. Comparing 88 and 10 differentially expressed pro⁃ teins, 9 proteins were the same. Among 88 differentially expressed proteins, 30 of them showed a direct interaction, and can be positioned in 13 signaling pathways based on KEGG and STRING software. Fourteen (46.67%) of the 30 proteins were lo⁃ cated in the complement and coagulation cascade pathway. Among 39 differentially expressed proteins, which were detected between No-ACR group and control group, 10 proteins showed a direct interaction including 9 proteins concentrated in the complement and coagulation cascade pathway. Conclusion By proteomic analysis, nine differentially expressed proteins are obtained, which may be regarded as the candidate bio-markers for ACR early diagnosis after liver transplantation. The complement and coagulation cascades system is significantly adjusted after liver transplantation, indicating this pathway plays an important role in the occurrence of ACR. Related Articles | Metrics

Select

Effects and mechanism of interferon-gamma on vasculogenic mimicry of melanoma cells HAN Jian， SUN Baocun,MA Yuemei,ZHAO Xiulan 2015, 43 (5):  453-456.  doi: 10.11958/j.issn.0253-9896.2015.05.002 Abstract ( 1024 )   PDF (948KB) ( 3960 )   Abstract： Objective To investigate the effects of interferon-gamma (IFN-γ) on migration， invasion and vasculogenic mimicry (VM) formation of human melanoma cell line MUM-2B. Methods MUM-2B cells were divided into three groups, control group (10%FBS in DMEM), treatment group1 (10 μg/L IFN-γ) and treatment group2 (100 μg/L IFN-γ). Different concentrations of IFN-γ were added in the culture medium of MUM-2B cells. Wound- healing assay and matrigel invasion assay were performed to examine the migration and invasion ability of MUM-2B cells. Three-D culture was used to observe the VM formation. The expression of vascular endothelial growth factor (VEGF) of MUM-2B cells was detected by Western blot assay. Results The result of wound-healing assay showed that the migration distance of cells was decreased in treat⁃ ment groups compared with that of control group. The migration distance of cells was decreased in treatment group2 com⁃ pared with that of treatment group 1(P < 0.05). The result of matrigel invasion assay showed that the number of invaded cells was decreased in treatment groups compared with that of control group, and which was significantly decreased in treatment group2 than that of treatment group1 (P < 0.05). The result of 3-D culture showed that cells in control group can form typical VM tube-like structures, whereas cells in treatment groups cannot. Western blot assay showed that the expression of VEGF protein was significantly decreased in treatment groups compared with that of control group, and the expression of VEGF pro⁃ tein was significantly decreased in treatment group2 than that of treatment group 1(P < 0.05). Conclusion These data sug⁃ gest that IFN-γ inhibits migration and invasion of MUM-2B cells, and inhibits VM formation by down regulating VEGF ex⁃ pression in vitro. Related Articles | Metrics

Select

GLP-1 regulates proliferation, differentiation and apoptosis of endothelial progenitor cells isolated from human umbilical cord blood by targeting the SDF-1/CXCR4 signaling pathway LIU Feng,XU Wenqiong， MIN Na, TANG Jiazhen， HUANG Haihua 2015, 43 (5):  457-460.  doi: 10.11958/j.issn.0253-9896.2015.05.003 Abstract ( 954 )   PDF (506KB) ( 3911 )   Abstract: Objective To investigate the molecular regulatory mechanism of glucagon like peptide 1 (GLP-1) on prolif⁃ eration, differentiation and apoptosis of human umbilical cord blood endothelial progenitor cells (EPCs). Methods EPCs were isolated from the umbilical cord blood of healthy pregnant women and cultured in 6- hole cell plate at 2×105 density in vitro, transfected with empty vector plasmid (control group), pcDNA3-GLP-1 plasmid (GLP-1 group), pcDNA3-GLP-1plas⁃ mid +AMD3100 (GLP-1+AMD3100 group) and simple AMD3100 (AMD3100 group). The pcDNA3-GLP-1 was transfected into EPCs. The 25μmol/L AMD3100 was used to block the SDF-1/CXCR4 signal pathway of EPCs for 1 h. The cell prolifera⁃ tion was determined by MTT method. The mRNA expressions of differentiation and apoptosis related genes PPARγ, C/EBPα and Caspase-3 were investigated by RT-PCR, and Caspase-3 activity was determined by Caspase-3 activity assay kit. Re⁃ sults Compared to control group, AMD3100 inhibitor showed no effects on cell proliferation, differentiation and apoptosis, while over-expression of GLP-1 in EPCs obviously promoted cell proliferation, and differentiation related genes PPARγ and C/EBPα mRNA expression, but down-regulated mRNA expression and the activity of Caspase-3 significantly (P < 0.05), in⁃ dicating that GLP-1 increased proliferation and differentiation of EPCs while decreased cell apoptosis. When the SDF-1/CX⁃ CR4 signaling pathway was blocked by AMD3100, over-expression of GLP-1 induced promotion of cell proliferation, and the differentiation was decreased significantly and the apoptosis was significantly increased (P < 0.05). Conclusion These data confirm that GLP-1 might promote EPCs proliferation and differentiation, and inhibit cell apoptosis through the regula⁃ tion of the SDF-1/CXCR4 signaling pathway. Related Articles | Metrics

Select

Effects of exenatide on the cell proliferation, invasion and apoptosis of human tongue squamous cell carcinoma SCC-25 HUANG Chao1, SHEN Feifei2, LI Gang3, ZHAO Yue4△ 2015, 43 (5):  461-464.  doi: 10.11958/j.issn.0253-9896.2015.05.004 Abstract ( 826 )   PDF (365KB) ( 3885 )   Abstract: Objective To detect the effects of exenatide on the related indicators of proliferation, invasion and apopto⁃ sis of cell line SCC-25. Methods SCC-25 cells were cultured in vitro. The expression level of glucagon like peptide 1 re⁃ ceptor (GLP-1R) was determined by Western blot assay in SCC-25 cells. SCC-25 cells were divided into four groups: con⁃ trol group and exenatide group (1, 10 and 100 nmol/L). The ability of cell proliferation was detected using MTT assay after 24 h, 48 h and 72 h of culture. The ability of invasion was measured with Transwell assays. The expression levels of MMP- 2, Caspase-3 and Phospho-p38 MAPK were measured by Western blot assay. Results GLP-1 receptor expression was found in SCC-25 cells. Compared with control group, the cell survival rate, invasion rate and the expression of MMP-2 were signifi⁃ cantly decreased in SCC-25 group (P < 0.05). The expression of Caspase-3 were significantly increased (P < 0.05).Changes were in a concentration-dependent and time-dependent manner (P < 0.05). The expression of Phospho-p38 MAPK was sig⁃ nificantly increased at 24 h in 10 nmol/L exenatide group (P < 0.05). Conclusion Exenatide can inhibit the cell prolifera⁃ tion and invasion, which may contribute the apoptosis by promoting expressions of Phospho-p38 MAPK and Caspase-3 of SCC-25 cells. Related Articles | Metrics

Select

Protective effects of schisandrin B on hypoxia injury of HK-2 cells LU Ailong, TAN Xiaoyue, ZHANG Mianzhi, WU Yinna 2015, 43 (5):  465-469.  doi: 10.11958/j.issn.0253-9896.2015.05.005 Abstract ( 598 )   PDF (589KB) ( 3891 )   Abstract： Objective To explore the protective effects of schisandrin B (Sch B) on hypoxia injury induced by cobal⁃ tous chloride (CoCl2) in human proximal renal tubular epithelial (HK-2) cells, and the possible mechanism thereof. Meth⁃ ods HK-2 cells were randomly assigned to four groups: control group (Con, cells were untreated), CoCl2 group (CoCl2, cells were treated with 600 μmol/L CoCl2 for 24 h), Sch B pretreat group (CoCl2+Sch B, cells were pretreated with 1 μmol/L and 10 μmol/L Sch B for 2 h) and Sch B group (Sch B, cells were treated with 1 μmol/L and 10 μmol/L Sch B for 2 h). CCK-8 kit was used to detect the cell viability of four groups. Flow cytometry was used to detect the apoptotic rate of four groups. The protein expression of hypoxia-inducible factor 1α (HIF-1α) was assessed by Western blot assay. The expressions of HIF-1α and inducible nitric oxide synthase (iNOS) mRNA were determined by RT-PCR. Results Compared with the control group, after treated with 600 μmol/L CoCl2, the cell viability was decreased, and the apoptosis was increased, the expressions of HIF-1α and iNOS mRNA were up-regulated in HK-2 cells. There was no significant difference in the expression of HIF- 1α mRNA between control group and CoCl2 group. Compared with the CoCl2 group, after pretreated with 1 μmol/L and 10 μmol/L Sch B, the cell viability was increased and the apoptosis was decreased, the expressions of HIF-1α and iNOS were down-regulated in HK-2 cells. There were no significant differences in the cell viability and apoptotic rate between control group and Sch B group. Conclusion Pretreatment with Sch B can reduce the apoptosis of HK-2 cells by inhibiting the ex⁃ pression of HIF-1α and iNOS mRNA, which shows protective effects on hypoxia injury. Related Articles | Metrics

Select

Effects of paeonol on RAS occurred on the development of ventricular remodeling after acute myocardial infarction in rats GU Yuanyuan, ZHOU Xiaohui, XU Qian, ZHAO Jingyi 2015, 43 (5):  470-473.  doi: 10.11958/j.issn.0253-9896.2015.05.006 Abstract ( 544 )   PDF (766KB) ( 3778 )   Abstract: Objective To investigate the effects of paeonol on renin-angiotensin system (RAS) occurred on the devel⁃ opment of ventricular remodeling after acute myocardial infarction (AMI) in rats. Methods The left anterior descending cor⁃ onary artery was ligated to establish the model of AMI in male SD rats. Six groups were set up: sham-operation group, AMI model group, captopril control group, paeonol low dose group (6 mg/kg), paeonol middle dose group (9 mg/kg) and paeonol high dose group (12 mg/kg). Rats were given treatment for 4 weeks after the AMI model was established. HE staining was used to observe changes of myocardial tissue. Real-time PCR was used to detect the mRNA levels of angiotensinogen (AGT), angiotensinⅡreceptor type1(AGTR1) and endothelin (ET)-1 of six groups. Western blot assay was used to detect the protein levels of peptidyl-dipeptidase A (ACE), angiotensin Ⅱ(Ang)-Ⅱand AGTR1 in six groups. Results The transcription of AGT, AGTR1, ET-1mRNA and the expressions of ACE, Ang-Ⅱand AGTR1 protein were significantly higher in myocardial tissue of AMI rats than those of sham-operation rats (P < 0.05). Compared with model group, the expressions of AGT, AG⁃ TR1, ET-1mRNA and ACE, Ang-Ⅱ, AGTR1 protein were significantly decreased in paeonol high dose group and captopril control group (P < 0.05). Paeonol reduced the expressions of those mRNA and protein levels in a significant dose dependent manner. Conclusion Paeonol can slow down the deterioration of the ventricular remodeling after AMI in rats, which may be related to the inhibition of over-activation of RAS. Related Articles | Metrics

Select

Effects of zonisamide on NO content and NOS activity in serum and brain tissue of epileptic rats CHEN Fang， LIU Bin， ZHANG Jing， WANG Wei， SUN Suzhen， WANG Lihui 2015, 43 (5):  474-476.  doi: 10.11958/j.issn.0253-9896.2015.05.007 Abstract ( 733 )   PDF (718KB) ( 3952 )   Abstract: Objective To investigate the effect of zonisamide as a new antiepileptic drug on nitric oxide (NO) content and nitric oxide synthase (NOS) activity in serum and brain tissue of epileptic rats. Methods Eight healthy rats were used as normal control group, and twenty- four epileptic rats induced by pentrazol were randomly divided into epilepsy model group, zonisamide group and phenobarbital group. Levels of NO and malondialdehyde (MDA) content, NOS and superoxide dismutase (SOD) activity in serum and brain tissue were detected in four groups. Results Forty-two rats were injected pen⁃ trazol, and 35 (83%) rats were established the rat model successfully. Epileptic waves were visible in EEG of epileptic rats. The concentrations of NO, MDA and the activity of NOS in serum and brain were significantly increased, the activity of SOD was significantly decreased, in epileptic rats than those of control rats. The concentrations of NO and MDA were significantly increased; the activity of SOD was significantly decreased, in brain in phenobarbital group compared with those of control group. There were significantly lower levels of NO, MDA and NOS, and significantly higher level of SOD in serum and brain tissue in zonisamide group and phenobarbital group than those of epileptic model group (P＜0.05). Conclusion Zonisamide plays an antiepileptic role by reducing the concentration of NO in brain of epileptic rats. Related Articles | Metrics

Select

Study of sanfukechuangao acupoint application for treatment of asthma in guinea pigs XIONG Xiangming 2015, 43 (5):  477-479.  doi: 10.11958/j.issn.0253-9896.2015.05.008 Abstract ( 803 )   PDF (778KB) ( 4508 )   Abstract: Objective To observe the effect of sanfukechuangao acupoint application on changes of interleukin- 18 (IL-18), eosinophils (EOS) and pulmonary pathology in asthma model of guinea pigs, and its underlying mechanism thereof. Methods A total of 60 guinea pigs were randomized into normal control group, asthma model group, acupoint-application 1 h group, acupoint-application 3 h group, acupoint-application 6 h group and dexamethasone group. Asthma model was es⁃ tablished by intraperitoneal injection of 10% ovalbumin (OVA， 1 mL) and forced inhalation of atomized 2% OVA. Skin prep⁃ aration and external application of Chinese medicinal herbs were made on back of guinea pigs, and "Feishu", "Xinshu", "Ge⁃ shu"were selected in acupuncture points). Levels of IL- 18 and EOS were detected by immunohistochemisty method. Re⁃ sults Compared with model group， the levels of IL-18 and EOS were significantly decreased, the pathological changes were significantly reduced in three acupoint- application groups. The effectiveness of acupoint-application 3 h group was the best in three groups. Conclusion Sanfukechuangao acupoint application can influence the levels of IL-18 and EOS effectively, and relieve phlegmonosis injuries in pulmonary tissue of asthma model of guinea pig. Related Articles | Metrics

Select

Effects of AG490 on the apoptosis of pancreatic acinar cells in rat model of severe acute pancreatitis FU Jinpeng, SONG Baoji, XIAO Yuanting 2015, 43 (5):  480-483.  doi: 10.11958/j.issn.0253-9896.2015.05.009 Abstract ( 595 )   PDF (703KB) ( 3909 )   Abstract: Objective To investigate the effect of AG490 on the apoptosis of pancreatic acinar cells and expression of apoptosis gene FasL and Bcl-2 in rat model of severe acute pancreatitis (SAP). Methods Seventy-two healthy Wistar rats were randomly divided into control group (n=24), SAP group (n=24) and AG490 group (n=24). Heart blood samples were tak⁃ en to detect serum amylase at 6 h, 12 h and 24 h after operation in three groups. Pancreatic tissue were observed under light microscope to analyze pathological changes and pathological scores. The index of pancreatic acinar cell apoptosis was detect⁃ ed by TUNEL. The expressions of FasL and Bcl-2 mRNA in pancreatic tissue was detected by RT-PCR. Results Com⁃ pared with control group, the damage of pancreatic tissue was gradually increased, the serum level of amylase significantly in⁃ creased (P＜0.01), the index of pancreatic acinar cell apoptosis increased, the expression of FasL mRNA was significantly in⁃ creased, the expression of Bcl- 2 mRNA was significantly decreased (P＜0.05 or P＜0.01) in SAP group. Compared with SAP group, the pancreatic injury was improved significantly, the serum amylase significantly decreased (P＜0.01), the apop⁃ tosis index rate of pancreatic acinar cells was increased, the expression of FasL mRNA was significantly increased, and the expression of Bcl-2 mRNA was significantly decreased (P＜0.05 or P＜0.01) in AG490 group. Conclusion The inhibition of the JAK/STAT3 signaling pathway may regulate the apoptosis-related gene to increase the apoptosis of pancreatic acinar cells, thereby reducing the reaction and pathological damages of acute pancreatitis. Related Articles | Metrics

Select

Apelin-13 protects the cerebral ischemia-reperfusion injury in rats DONG Xin, LU Suqing, LIAO Huiying, OUYANG Xinping, LI Guoshu, ZHOU Jie 2015, 43 (5):  484-487.  doi: 10.11958/j.issn.0253-9896.2015.05.010 Abstract ( 786 )   PDF (434KB) ( 3858 )   Abstract: Objective To observe the protective effect of Apelin- 13 on the cerebral ischemia- reperfusion injury (CIRI), and to explore the possible mechanism in rat model. Methods Fifty male SD rats were randomly divided into five groups: sham group, CIRI model group and Apelin-13 (0.1, 1.0 and 10.0 μg/kg) treatment groups. The model of CIRI was es⁃ tablished by filament. After 2 h ischemia, the focal middle cerebral artery was followed by 72 h reperfusion. Apelin-13 was administrated by intracerebroventricular injection 30 minutes before reperfusion. The score of neural function was estimated in different time points. The 2,3,5- triphenyl tetrazolium chloride (TTC) dye was used to calculate the volume and percent⁃ age of cerebral infarction. The endoplasmic reticulum stress (ERS) protein markers including glucose-regulated protein 78 (GRP78) and CCAAT/enhancer binding protein homologous protein (CHOP) in cerebral cortex were measured by Western blot assay. Results Compared with the sham group, the score of neural function was significantly increased, the infarct rate was reached(47.63 ± 5.81)% and the protein expressions of GRP78 and CHOP were significantly up-regulated in CIRI mod⁃ el group (P<0.05). There were no significant differences in these data between the CIRI model group and 0.1 μg/kg Apelin- 13 treatment group (P>0.05). Compared with the CIRI group, the neural function defect was significantly improved, the mus⁃ cle strength was significantly enhanced and the infarct rate was significantly decreased, and the protein expressions of GRP78 and CHOP were significantly down-regulated in the 1.0 and 10.0 μg/kg Apelin-13 treatment groups (P<0.05). Con⁃ clusion Apelin-13 protects the cerebral ischemia-reperfusion injury in rat model, which may be related with the inhibition of endoplasmic reticulum stress. Related Articles | Metrics

Select

Effects of PPARγ on malignant arrhythmia in myocardial ischemia/reperfusion model rats HE Xueshu, MO Xinling△, CHEN Hua, XIE Ting 2015, 43 (5):  488-490.  doi: 10.11958/j.issn.0253-9896.2015.05.011 Abstract ( 696 )   PDF (298KB) ( 3993 )   Abstract: Objective To investigate the effects of peroxisome proliferator activated receptor gamma (PPARγ) on ma⁃ lignant arrhythmia in myocardial of ischemia/reperfusion(I/R) rats. Methods Twenty-four SD rats were randomly divided into four groups: Sham group, I/R group, rosiglitazone (ROS) group and PPARγ inhibitor GW9662 (GW) group. The myocar⁃ dial I/R injury was induced by ligation of the left anterior descending coronary artery, with ischemia for 30 min and reperfu⁃ sion for 2 h. The whole process limb Ⅱ lead electrocardiogram was applied to observe the frequency of malignant arrhythmia and record the corrected changes of QT(QTc) interval. RT-PCR was used to detect the expression of PPARγ mRNA. Re⁃ sults There were 5 cases of malignant arrhythmia in ROS group, 2 in I/R and 1 in GW group, 0 was found in Sham group. There was a prolongation of the QTc interval in ROS group than the other groups after ischemic stage (P < 0.05). Compared with I/R group and ROS group, the QTc intervals were shorten in ischemia 30 min and reperfusion process in GW group (P< 0.05). Compared with sham group, the expression of PPARγ mRNA was significantly increased in other three groups (P< 0.05). The expression level of PPARγ mRNA was the highest in ROS group. The expression level of PPARγ mRNA was re⁃ duced in GW group compared with that of I/R group and ROS group (P < 0.05). Conclusion Over expression of PPARγ may lead to the occurrence of malignant arrhythmia in myocardial ischemia/reperfusion rats. Related Articles | Metrics

Select

Therapeutic effects of Di’ ao Xinxuekang on myocardial ischemia-reperfusion injury in rats ZHENG Haijuan1,2, WANG Weiting2, HAO Chunhua, ZHAO Zhuanyou2, TANG Lida2△ 2015, 43 (5):  491-495.  doi: 10.11958/j.issn.0253-9896.2015.05.012 Abstract ( 969 )   PDF (507KB) ( 4011 )   Abstract:Objective To observe the therapeutic effect of Di’ ao Xinxuekang (DAXXK) on myocardial ischemia-reper⁃ fusion injury in rats, and to explore its mechanisms. Methods The myocardial ischemia-reperfusion injury model was es⁃ tablished by the ligation of descending coronary artery in rats. Then animals after the modeling were randomized into model group, DAXXK-d (31.5 mg/kg) group, DAXXK-g (63.0 mg/kg) group and Diltiazem (24.8 mg/kg) group. A separate sham group was used as control. The treatment group was given DAXXK once a day for 7 days. Cardiac function and cardiac config⁃ uration were measured by color Doppler ultrasound diagnostic method. Hemodynamics was measured by Millar catheter method. The arterial oxygen saturation and blood oxygen pressure were measured by i-STAT 300 blood gas analyzer. Inflam⁃ matory cytokines interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, adhesion molecules VCAM-1, ICAM-1, and apoptosis-related protein Bcl-2, Bax were detected by ELISA. Myocardial apoptosis was measured using TUNEL method. Results Compared with model group, the left ventricular fractional shortening (FS), the systolic and diastolic function were improved, and the left ventricular pressure maximum rise / fall rates (± LVdp/dtmax) were increased, in DAXXK group. DAXXK improved lung function, increased arterial oxygen pressure and oxygen content. The inflammatory cytokines IL-1β, IL-6, TNF-α and adhesion molecules ICAM-1 and VCAM-1 were decreased in DAXXK group. The myocardial swelling and inflammatory infiltration were relieved, myocardial apoptosis was reduced, the expression of Bcl-2 protein was increased and the expression of Bax protein was decreased in DAXXK group. Conclusion DAXXK can protect myocardial ischemiareperfusion injury, which involved in the inhibition of apoptosis and reduction of inflammatory cytokines. Related Articles | Metrics

Select

The clonal origin of diffuse hepatocellular carcinoma ZHANG Luzhou1, WANG Zhenglu2,3, WANG Yuliang2,4, ZHENG Hong 2,3? 2015, 43 (5):  496-499.  doi: 10.11958/j.issn.0253-9896.2015.05.013 Abstract ( 683 )   PDF (429KB) ( 3810 )   Abstract： Objective To determine the tumor clonal origin of diffuse hepatocellular carcinoma (D-HCC) and clinical outcome after liver transplantation. Methods Fourteen D-HCC patients who underwent liver transplantation in the Tianjin First Central Hospital between August 2004 to November 2012 were evaluated retrospectively. Their clinical characteristics and data of disease-free survival postoperatively were gathered. Twelve loss of heterozygosity sites (LOH) on high frequency microsatellite were selected. The tumor clonal origin of D-HCC was assessed by the means of PCR-SSCP. Results Of 14 D-HCC patients, intrahepatic metastasis (IM) occurred in 11 cases (11/14), and IM with multicentric occurrence (MO) oc⁃ curred in 3 cases (3/14). The disease-free survival time was 4.5 to 37.4 months, and the median time was 8.7 months. Con⁃ clusion The most frequent tumor clonal origin of D-HCC was IM. Liver transplantation may be the effective treatment for D-HCC. Related Articles | Metrics

Select

The association of DNMT3a, DNMT3b expression with the state of promoter methylation of ERα gene and ERα expression in sporadic breast cancer WANG Yong1, WANG Xiaodong2, CHEN Wenjie2, YU Zhaojin2, WU Huizhe2, ZHAO Lin2， WEI Minjie2△ 2015, 43 (5):  500-504.  doi: 10.11958/j.issn.0253-9896.2015.05.014 Abstract ( 1034 )   PDF (579KB) ( 4208 )   Abstract： Objective To investigate the correlationship between DNMT3a, DNMT3b protein expressions and the state of promoter methylation of ERα gene and ERα protein expression in the development of sporadic breast cancer. Methods A total of 180 patients with sporadic breast cancer and 30 patients with breast fibroadenoma were included in this study. The expressions of DNMT3a and DNMT3b protein were detected by immunohistochemical method. The state of promoter methyla⁃ tion of ERα gene was detected by methylation specific PCR in 97 patients with sporadic breast cancer. Results There were no significant differences in positive expression rates of DNMT3a and DNMT3b protein between breast fibroadenoma and breast cancer. There were higher expression levels of DNMT3a and DNMT3b in breast cancer patient of Ⅲ~Ⅳ stages than those ofⅠ~Ⅱstages. The expression of DNMT3a was significantly higher in patients with lymph node metastasis than that of patients without lymph node metastasis (P < 0.05). Of 97 cases of breast cancer patients, ERα gene promoter methylation oc⁃ curred in 39 cases (40.2%). The positive expression of DNMT3a protein was positively correlated with the ERα gene methyla⁃ tion (rS=0.250). The DNMT3a protein expression showed a significant influence to the overall survival (OS) in patients of breast cancer (P=0.035), no significant influence to the disease-free survival (DFS) (P=0.064). DNMT3b protein expression showed no significant influence to OS and DFS of patients with breast cancer (P = 0.914 and 0.961). Conclusion The posi⁃ tive expressions of DNMT3a and DNMT3b are correlated with the invasion, metastasis and poor prognosis of sporadic breast cancer. DNMT3a was positively correlated with the state of ERα gene promoter methylation. The inhibition of DNMT3a and DNMT3b may have advantages in the prevention and treatment of sporadic breast cancer. Related Articles | Metrics

Select

The analysis on factors related to BMD in patients with type 2 diabetes CAO Guolei, LI Jun, LI Siyuan, HOU Junxia, YANG Yi 2015, 43 (5):  505-507.  doi: 10.11958/j.issn.0253-9896.2015.05.015 Abstract ( 693 )   PDF (287KB) ( 3811 )   Abstract: Objective To explore related factors affecting bone mineral density (BMD) and osteoporosis (OP) in pa⁃ tients with type 2 diabetes mellitus (T2DM). Methods Dual-energy X-ray absorptiometry was used to measure BMD of the femoral neck and the lumbar vertebrae in 220 patients with T2DM. All the patients were divided into three groups: normal bone mass group, decreased bone mass group and OP group according to the value of BMD. The clinical data and biochemi⁃ cal indicators were compared and analyzed between these groups, and the influencing factors of T2DM and OP were ex⁃ plored. Results Compared with normal bone mass group and decreased bone mass group, there were more older patients, longer course of T2DM, lower BMI and high density lipoprotein cholesterol (HDL- C), and higher glycated hemoglobin (HbA1c) level in OP group. There were significantly higher age, longer course of T2DM, higher level of HbA1c, and lower levels of BMI, HDL-C in decreased bone mass group than those of normal bone mass group (P<0.05). There were no signifi⁃ cant differences in waist-to-hip ratio, systolic blood pressure (SBP), diastolic blood pressure (DBP), postmenopausal women (PMW) ratio, fasting plasma glucose (FPG), 2-h postprandial blood glucose (2hPG), blood phosphorus, calcium, total choles⁃ terol (TC), three acyl glycerin (TG) and low-density lipoprotein cholesterol (LDL-C) between three groups. The risk factors for T2DM combined with OP were older, female, long duration of T2DM, higher levels of FPG, 2hPG and HbA1c. Conclu⁃ sion Older and female T2DM patients were high-risk group of OP. The treatment plan should be timely adjusted by regular⁃ ly monitoring indexes of blood glucose and HbA1c. Related Articles | Metrics

Select

Pathogenic analysis of acute lower respiratory infections and its correlation with asthma exacerbations FU Zhuo1, WAN Liya1, XU Yongsheng1, GUO Wei1, ZHENG Yuejie2△ 2015, 43 (5):  508-510.  doi: 10.11958/j.issn.0253-9896.2015.05.016 Abstract ( 731 )   PDF (276KB) ( 4603 )   Abstract: Objective To investigate the prevalence of year-round respiratory viral infection in children with lower re⁃ spiratory tract infection (LRTI), and the relationship between respiratory viral infection and allergen sensitization in exacer⁃ bating asthma. Methods A total of 231 hospitalized children with acute LRTI were investigated from May 2013 to April 2014. The 5 most common respiratory viruses were isolated from nasopharyngeal aspirate using multiplex reverse transcrip⁃ tion-polymerase chain reaction, including respiratory syncytial virus (RSV), adenovirus (AV), parainfluenza virus (PIV), in⁃ fluenza virus (IFV) and rhinovirus (RV). Atopic sensitization was defined if more than 1 serum specific immunoglobulin E level measured using immunofluorescence experiment was over 0.35 IU/mL. Results RSV was the most common pathogen of bronchiolitis in hospitalized children through the year. RV or IFV infections were more prevalent in asthma exacerbations compared to other LRTIs. AV was more likely to cause pneumonia. RV and IFV were associated with asthma exacerbations in children with atopic sensitization, but not in nonatopic children. Conclusion RV and IFV are associated with hospital⁃ ization for asthma exacerbation in children with atopic sensitization. Related Articles | Metrics

Select

Relationship of the fat metabolic parameters and androgen level of umbilical cord blood in newborns of mothers with polycystic ovary syndrome HUANG Longjian， LYU Yan， ZHENG Jinghui， CHEN Jingxia 2015, 43 (5):  511-514.  doi: 10.11958/j.issn.0253-9896.2015.05.017 Abstract ( 647 )   PDF (319KB) ( 4272 )   Abstract:Objective To assess the relationship between fat metabolic parameters and androgen concentration in the cord blood of newborns of mothers with polycystic ovary syndrome (PCOS). Methods This cross-sectional study included PCOS women (n=55) and neonatal, and 40 cases with matched body mass index (BMI) were used as control. The clinical data including height, body mass, waist circumference, hip circumference of PCOS group, and length and head circumference in newborns after delivery were measured and compared. Blood lipid level, serum insulin and testosterone level were detect⁃ ed using umbilical artery-vein mixed cord blood after delivery. Regression analysis was used to analyze the influence factors of neonatal cholesterol and testosterone levels. Results The neonatal birth weight, head circumference, cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol and triglyceride level were significantly lower, birth height and testosterone level were significantly higher, in PCOS group than those of control group (P < 0.05). Values of waist to hip ratio, BMI, cholesterol, high density lipoprotein cholesterol and testosterone levels were significantly higher in PCOS group than those of control group (P < 0.05). The insulin, cholesterol and triglyceride levels of PCOS mother were risk factors for neonatal cholesterol level(P < 0.05). The cholesterol, triglyceride and free testosterone levels of PCOS mother were risk factors for increased neonatal free testosterone (P < 0.05). Conclusion Mother with PCOS may affect fetal birth weight， head circumference and cord blood lipid metabolism, which may be related with the elevated level of testosterone during the fetal period. Related Articles | Metrics

Select

Risk factors for acute kidney injury after cardiopulmonary bypass operation in children with non cyanotic congenital heart disease WEN Yupeng, CHANG Cheng, WANG Zhonglun, ZHENG Jie 2015, 43 (5):  515-517.  doi: 10.11958/j.issn.0253-9896.2015.05.018 Abstract ( 669 )   PDF (277KB) ( 4323 )   Abstract： Objective To investigate the incidence and risk factors of acute kidney injury (AKI) after cardiopulmonary bypass(CRB) in children with non cyanotic congenital heart disease. Methods A total of 150 children who underwent car⁃ diopulmonary bypass operation from 2012 May to 2014 May were analyzed retrospectively. The patients were divided into two groups: AKI group (n=29) and non AKI group (n=121). Clinical data were collected and compared between two groups, including gender, age and baseline serum creatinine level. Multivariate Logistic regression analysis was used to evaluate the risk factors for AKI after operation. Results There was no death in the 150 cases. Comparison between groups, there were significant differences in age, history of heart failure and (or)pneumonia, baseline serum creatinine level, baseline uric acid, body mass, CPB) time, aortic clamping time, mechanical ventilation time, postoperative transfusion and hypotension. Multi⁃ variate Logistic regression analysis showed that age (≤1 years old), the history of pneumonia and (or) heart failure, CPB time (>40 min), aortic clamping time (>30 min) were the independent risk factors for AKI after operation. Conclusion It is im⁃ portant to choose the best age for surgery in children, actively prevent preoperative complications and shorten the CPB and aorta blocking time for preventing the occurrence of AKI. Related Articles | Metrics

Select

In vitro biomimetic remineralization of dentin collagen by phosphorylated chitosan/amorphous calcium phosphate compound and tripolyphosphate MIAO Ruijing， ZHANG Xu， SUN Yingchun， GAO Ping△ 2015, 43 (5):  518-521.  doi: 10.11958/j.issn.0253-9896.2015.05.019 Abstract ( 554 )   PDF (881KB) ( 4245 )   Abstract： Objective To investigate the remineralizing therapy of deep caries and in vitro biomimetic remineralization of demineralized dentin by phosphorylated chitosan/amorphous calcium phosphate compound (P-chi/ACP) and tripolyphos⁃ phate (TPP). Methods Thirty-two extracted human molars were cut and completely demineralized. Two samples were used to show the characteristics by transmission electron microscopy (TEM). The other 30 samples were divided into two groups: fifteen samples were treated by P-chi/ACP and TPP (P-chi/ACP+TPP group), the other fifteen samples were not treated by TPP (P-chi/ACP group). All of the samples were distinguished into experimental side and control side, and then they were set on the in vitro model for 1 week. Micro-computed tomography (μCT) and TEM were used to assess the effects of reminer⁃ alization. Results μCT detection revealed that the mineral density were higher in the experimental sides (125.42±12.16 and 119.39±8.64) than that of control sides (96.96±10.56 and 105.27±9.42) in both groups (P < 0.01). TEM figures showed that hierarchical intrafibrillar remineralization was realized in samples of P-chi/ACP+TPP group, while trace amounts of hi⁃ erarchical remineralization was detected in P-chi/ACP group. Conclusion Fully demineralized dentin appears to have the potential to be remineralized with the application of P-chi/ACP. The ultrastructure of samples is better in P-chi/ACP+TPP group than that of P-chi/ACP group. Related Articles | Metrics

Select

Study on antibacterial ability of silver nanoparticles loaded titanium nanotubes MIAO Jingwen, ZHANG Xu, ZHANG Wenyi, SUN Yingchun, MA Shiqing, GAO Ping△ 2015, 43 (5):  522-525.  doi: 10.11958/j.issn.0253-9896.2015.05.020 Abstract ( 640 )   PDF (3928KB) ( 3818 )   Abstract: Objective To detect the inhibitory effect of siliver nanoparticles loaded titanium nanotubes on staphylococ⁃ cus aureus, and provide a theoretical basis for implant local application. Methods Orderly arrangement of titania nano⁃ tubes produced by anodic oxidation, loaded silver nanoparticals by situ replacement. Scanning electron microscopy (SEM) and transmission electron microscopy(TEM) were used to detect the morphology topology of silver nanoparticals, titanium nanotubes and siliver particals loaded titanium nanotubes. The minimum inhibitory concentration of silver nanoparticles was calculated. The antibacterial of planktonic bacteria was detected 1 day, 3 days and 5 days after culturing staphylococcus au⁃ reus on siliver particals loaded titanium nanotubes. The inhibitory bacterial adhesion properties were detected by scanning electron microscopy. Results The uniform and orderly diameter of 80~120 nm TiO2 nanotubes were prepared under 18 V voltage, loaded diameter of 20 nm silver nanoparticals, which effectively inhibited adhesion and proliferation of staphylococ⁃ cus aureus. Conclusion Titanium nanotubes produced by 18 V have a stronger drug loading capacity. The 100 mmol/L sil⁃ ver nanopartical solution loaded nanotubes can effectively inhibit staphylococcus aureus adhesion and proliferation within three days. Related Articles | Metrics

Select

The study of cytocompatibility of Co-Cr alloy and Ti alloy YAN Tao 2015, 43 (5):  526-528.  doi: 10.11958/j.issn.0253-9896.2015.05.021 Abstract ( 720 )   PDF (318KB) ( 3848 )   Abstract: Objective To investigate the cytocompatibility of the liching liquids of Co-Cr alloy and Ti alloy on human gingival fibroblasts (HGF). Methods The HGF were treated in vitro with leaching liquids of Co-Cr alloy and Ti alloy, re⁃ spectively. The DMEM cell medium containing 10% fetal bovine serum was served as a negative control. The viability of HGF treated by two dental alloys were evaluated by means of MTT, and the contents of intracellular reduced glutathione (rGSH) were assayed by kits. The tumor necrosis factor-α (TNF-α) contents were determined in the culture supernatant by ELISA in two groups. The effects of these alloys on the expression of caspase-3 were examined by real time-PCR method. Results Compared with the control group, HGF treated with Co-Cr alloy leaching liquids showed a lower viability (P < 0.05), while Ti alloy leaching liquid promoted the proliferation of HGF. In Co-Cr alloy group, the rGSH content was signifi⁃ cantly decreased (P < 0.05), while TNF-α content was significantly increased (P < 0.05) compared with control group. There were no significant differences in rGSH and TNF-α contents between the Ti alloy group and control group (P > 0.05). The ex⁃ pression of caspase-3 was significantly higher in Co-Cr alloy group than that of control group (P < 0.05), while there was no significant difference in the expression of caspase-3 between Ti alloy group and control group. Conclusion Results suggest that Co-Cr alloy possesses cytotoxicity, while there is better cell compatibility for Ti alloy. Related Articles | Metrics

Select

Research on mandibular bone mineral density in patients with end-stage renal disease LIU Jianshan， LI Xiaoying，WANG Ying， WANG Shan， PENG Cheng△ 2015, 43 (5):  529-532.  doi: 10.11958/j.issn.0253-9896.2015.05.022 Abstract ( 620 )   PDF (437KB) ( 3854 )   Abstract：Objective To investigate the effectiveness of panoramic mandibular index (PMI) in detecting the bone min⁃ eral density (BMD) of mandibular bone and whole-body bone in patients with chronic end-stage renal failure. Methods A total of thirty patients with peritoneal dialysis treatment were used as experimental group and 31 healthy adults were used as control group. The panoramic jaw tomography was taken for the measurement of superior PMI (sPMI) and inferior PMI (iPMI) in two groups. The dual-energy X-ray absorptiometry (DXA) was used to detect BMD of lumbar spine bone. Data were com⁃ pared between two groups. The correlation of sPMI, iPMI and BMD of lumbar spine bone was analyzed. Results All indica⁃ tors including sPMI(0.262 2±0.026 7 vs 0.284 2±0.025 4, t=3.301) , iPMI (0.314 1±0.028 3 vs 0.334 1±0.027 5, t=2.808) and BMD of lumbar spine bone (0.832 3±0.101 0 vs 0.906 9±0.113 6，t=2.709) were significantly lower in experimental group than those in control group (P < 0.01). There was a positive correlation between sPMI and iPMI with BMD of lumbar spine bone (r=0.439 and 0.389, P < 0.05). The BMD of lumbar spine bone was significantly lower in female patients than that of male patients in control group (0.849 7±0.114 7 vs 0.968 0±0.076 3， t=3.357). The BMD of lumbar spine bone was also sig⁃ nificantly lower in female patients than that of male patients in experimental group (0.775 4±0.068 4 vs 0.882 1±0.099 9， t= 3.365). There were no significant differences in values of sPMI and iPMI between male and female patients of two groups. Conclusion The BMD of mandibular bone is lower in patients with chronic end-stage renal failure than that of the normal people. PMI index is a simple and effective method to detect the BMD of mandibular bone, which can reflect the BMD of whole body bone in patients with chronic end-stage renal disease. Related Articles | Metrics

Select

Study for gene mutation detection of circulating DNA with ligase-ELISA reaction CUI Haizhong, XIAO Na, ZHANG Yongping, CHEN Dagui, TANG Yitong 2015, 43 (5):  533-536.  doi: 10.11958/j.issn.0253-9896.2015.05.023 Abstract ( 504 )   PDF (414KB) ( 4012 )   Abstract： Objective To establish a single nucleotide polymorphisms genotyping (SNP) method for a convenient, accu⁃ rate, and routine analysis of clinical samples. Methods Based on the design of oligonucleotide probe, the assay was per⁃ formed through three steps: the conjunction of the detection probe, universal amplification, labeling and ELISA reaction. The genotype of each SNP was revealed by reading signals of each set of reaction tubes. This assay was applied to detect sixtytwo plasma samples of lung cancer for circulating DNA for three SNPs of EGFR, c.2573T>G（L858R） , EGFR, c.2582T>A （L861Q） , EGFR, c.2155 G>T （G719C). Results were compared with those obtained by direct sequencing. Results The het⁃ erozygote mutation was identified for L858R by both methods, although no mutation was detected for L861Q and G719C. Six samples were identified as heterozygotes with the new method, and only two samples were unambiguously identified as het⁃ erozygotes by the direct sequencing. Two additional samples could not be identified as heterozygotes because the peak of mu⁃ tant allele was very low compared with that of wild allele. Conclusion The developed method enabled accurate identifica⁃ tion of SNP in a convenient manner, and which is adapted to routine analysis from heterogeneous samples unambiguously. Related Articles | Metrics

Select

Effects of dexmedetomidine preconditioning on imbalance of nitric oxide/endothelin-1 and remote lung injury in patients with lower limb ischemia-reperfusion YU Jian， LI Rui， WANG Qi 2015, 43 (5):  537-541.  doi: 10.11958/j.issn.0253-9896.2015.05.024 Abstract ( 620 )   PDF (403KB) ( 4112 )   Abstract： Objective To investigate the effects of dexmedetomidine preconditioning on nitric oxide (NO)/endothelin (ET)-1 imbalance and remote lung injury induced by lower limb ischemia-reperfusion (LIR). Methods Sixty patients who scheduled for unilateral lower extremity surgery matched American Society of Anesthesiologists (ASA) Ⅰ-Ⅱ, were random⁃ ized into two groups: control group (R group, n=30) and dexmedetomidine preconditioning group (PD group, n=30). Lumbar plexus combined with sciatic nerve block was performed guided by a nerve stimulator in both groups. In group PD, dexme⁃ detomidine intravenous infusion was started at a dose of 0.125 mL /kg (4 mg/L) for 10 minutes before using tourniquet, where⁃ as group R received an equivalent volume of normal saline. Artery blood gas analysis, respiratory index and oxygenation in⁃ dex were measured, and NO, ET-1, interleukin-8 (IL-8) and malondialdehyde (MDA) concentrations were determined from plasma samples 10 minutes before tourniquet inflation (T0), 15 minutes (T1), 2 h (T2), 6 h (T3) and 24 h (T4) after tourniquet deflation. Results Compared with T0, RI was higher at T3 in group R and OI was lower at T2-4 (P < 0.01). There were no signifi⁃ cant differences in RI and OI of group PD between different time points (P > 0.05). In R and PD groups, ET-1, IL-8 and MDA concentrations were increased, while NO level and NO/ET-1 ratio were significantly decreased after tourniquet defla⁃ tion (P＜0.05). Compared with group R, RI was lower and OI was higher at T3 in group PD (P < 0.01). The levels of ET-1, IL-8, MDA, NO and NO/ET-1 ratio were significantly different after tourniquet deflation between group PD and group R (P＜0.05). In group R, there was positive correlation between ET-1 and IL-8 levels with RI, and negative correlation be⁃ tween ET-1 and IL-8 levels with OI (P < 0.01). There was positive correlation between NO level, NO/ET-1 ratio and RI lev⁃el (P <0.01). Conclusion Lung function impairment induced by tourniquet application could be attenuated by dexmedeto⁃ midine preconditioning based on protecting endothelial cells and inhibiting lipid peroxidation. Related Articles | Metrics

Select

Comparison of postoperative analgesic effect between the single dose of oxycodone and dezocine in patients undergoing gynecological laparoscopic operation SHEN Junmei, SONG Zixian, YONG Fangfang, ZHU Kangsheng， JIA Huiqun 2015, 43 (5):  542-544.  doi: 10.11958/j.issn.0253-9896.2015.05.025 Abstract ( 1010 )   PDF (285KB) ( 4093 )   Abstract: Objective To compare the postoperative analgesic effect of the single dose of oxycodone and dezocine in patients who underwent gynecological laparoscopic operation. Methods Sixty patients who underwent elective gynecologi⁃ cal laparoscopic operation were randomly divided into two groups (n=30): oxycodone group (group O) and dezocine group (group D). Fifteen minutes before the end of surgery, oxycodone 0.1 mg/kg was given in O group, and dezocine 5 mg was giv⁃ en to D group. Twenty minutes before the end of surgery, tropisetron 5 mg was given to both groups. Analgesia was main⁃ tained by propofol-remifentanil with TCI. The mean arterial pressure (MAP) and heart rate (HR) of T1, T2, T3 and T4 were recorded respectively in both two groups. After the operation, pain of visual analogue scale (VAS) was assessed in 2 h ,4 h , 6 h and 24 h, respectively. Results There were no significant differences in MAP and HR between two groups at T1, T2, T3 and T4 (P > 0.05). The VAS score was significantly lower in group O than that of group D (P < 0.05). There was significant difference in the incidence of nausea between the two groups (P < 0.05). Conclusion Single dose of oxycodone 0.1 mg/kg can be used for postoperative analgesia after gynecological laparoscopic operation, and which has better analgesia than that of dezocine, except for the adverse reaction of nausea. Related Articles | Metrics

Select

The nutritional survey analysis of hospitalized elderly patients with fractures ZHANG Caiyun, LEI Min, ZHANG Na, GUO Chunhua, JING Yongmin 2015, 43 (5):  545-547.  doi: 10.11958/j.issn.0253-9896.2015.05.026 Abstract ( 619 )   PDF (317KB) ( 3967 )   Objective To evaluate the nutritional status of elderly patients with fracture, used by mini nutritional assessment (MNA-SF). Methods Total 1353 cases of hospitalized elderly patients with fracture were included. The MNA-SF investigations were completed within 3 days after admission. The relationships among nutritional status and different ages, with or without exercise, different fracture site, with different diseases were analyzed. Results There were statistical significances about the sum of rates of malnutrition risk and malnutrition among different ages, between with and without exercise, between femoral intertrochanteric fracture and upper limb fracture (P=0.000). There were statistical significances about the sum of rates of malnutrition risk and malnutrition between patients with cardiovascular disease and two kinds of diseases, between patients with cardiovascular disease and three kinds of diseases, between cerebral vascular diseases and two kinds of diseases, between cerebral vascular diseases and three kinds of disease (P=0.001; P=0.001; P=0.001; P=0.004). Conclusion Patients aged 70~90 years old, with no regular exercise, intertrochanteric fracture, diabetes mellitus or a variety of chronic disease a higher incidence of malnutrition risk and malnutrition. Related Articles | Metrics

Select

Epidemiological analysis of reported emergency public health events at schools in Tianjin from 2006 to 2013 CHEN Qian, DONG Xiaochun, GOU Jinbo, SUN Meiling 2015, 43 (5):  548-551.  doi: 10.11958/j.issn.0253-9896.2015.05.027 Abstract ( 571 )   PDF (359KB) ( 4110 )   Abstract: Objective To analyze the epidemiological characteristics of reported emergency public health events at schools from 2006 to 2013, and provide a scientific basis for better disposal of school-related events. Methods The report⁃ ed emergency public health events at schools in Tianjin from 2006 to 2013 were collected, and descriptive epidemiological analysis was conducted on data. Results A total of 267 reported emergency public health events at school in Tianjin during 2006-2013, of which 257 infectious disease events, accounting for 96.25%. The incident occurred in primary schools and nurseries. The peak was from March to June and October to December for the incident. The top three species of disease onset were influenza (2 360 cases), chicken pox (2 032 cases) and mumps (1 813 cases). There was a correlation between the duration and interval from onset of the first case to report of emergency public health events（rs=0.522， P < 0.05). Conclusion In recent years, school public health emergency events are high momentum, mainly in rural areas, primary schools and nurseries. Timely detec tion and disposal of emergency public health events can reduce the harm generated by them. Related Articles | Metrics

Select

Association between maternal MTHFR C677T polymorphism and neural tube defects in offsprings: a Meta-analysis FANG Yulian, MA Shikun, SHI Ouyan, ZHANG Peng, CAI Chunquan 2015, 43 (5):  552-558.  doi: 10.11958/j.issn.0253-9896.2015.05.028 Abstract ( 742 )   PDF (912KB) ( 4043 )   Abstract： Objective To explore the association between maternal methylene tetrahydrofolate reductase (MTHFR) C677T polymorphism and neural tube defects (NTDs). Methods CBM, VIP, CNKI, Wanfang, PubMed and Web of Science databases from set up to March, 2014 were electronically searched to identify case-control studies on the relationship be⁃ tween maternal MTHFR C677T polymorphism and NTDs. The data were quantitatively analyzed by RevMan 5.0 software. Re⁃ sults A total of 25 studies were selected including 2 282 cases and 3 420 controls. Overall, the pooled OR (with 95% CI) under co-dominant model and allele contrast were 2.28(1.60-3.24), 1.25(1.02-1.53) and 1.42(1.21-1.67). Subgroup analy⁃ sis showed significant association between maternal MTHFR C677T polymorphism and NTDs susceptibility in Asian popula⁃ tions. Conclusion The present meta-analysis suggests that MTHFR C677T polymorphism is significantly associated with maternal risk for NTDs, especially in Asian populations. Related Articles | Metrics

Select

Glucocorticoid combined with mycophenolate mofetil versus cyclophosphamide in the treatment for adult refractory nephrotic syndrome:a Meta-analysis FENG Wei, TANG Rongwei, LIAO Yunhua 2015, 43 (5):  559-565.  doi: 10.11958/j.issn.0253-9896.2015.05.029 Abstract ( 706 )   PDF (624KB) ( 4630 )   Abstract：Objective To systematically review the efficacy and safety of mycophenolate mofetil versus cyclophospha⁃ mide in adults with refractory nephrotic syndrome. Methods The randomized controlled trials of mycophenolate mofetil and cyclophosphamide treatment for refractory nephrotic syndrome were searched from Cochrane library, PubMed, EMbase, Wanfang, VIP and CNKI till March 2014. The relevant studies were screened according to inclusion criteria and exclusion criteria. The quality of the included studies was evaluated. Meta-analyses were performed by using RevMan 5.2 software. The indexes were analyzed including the complete remission rate, efficiency, serum albumin, and adverse reaction after com⁃ pleting the treatment for adults with refractory nephrotic syndrome. Results There were 9 RCTs, a total of 467 patients were enrolled. The result of the meta-analysis showed that mycophenolate mofetil could significantly increase complete re⁃ mission rate (RR=1.45, 95% CI=1.17~1.81, P=0.000 7) and efficiency rate (RR=1.23, 95 % CI=1.11~1.36, P < 0.000 1). It can also enhance the level of serum albumin (WMD=2.73, 95% CI=1.42~4.04, P < 0.000 1) and decrease 24-hour urinary protein (SMD=-0.63, 95%CI=-1.16~-0.10, P=0.02) compared with cyclophosphamide in the treatment of refractory nephrot⁃ ic syndrome. There was no significant difference in the serum level of cholesterol between mycophenolate mofetil group and cyclophosphamide group (SMD=0.31, 95%CI=-0.23~0.84, P=0.26 ). The incidence rates of liver dysfunction (RR=0.13,95% CI=0.06~0.28, P < 0.000 01), leukopenia (RR=0.10, 95% CI=0.04~0.23, P < 0.000 01), gastrointestinal reaction (RR=0.21, 95% CI=0.11~0.39, P < 0.000 01) and alopecia (RR=0.08, 95% CI= 0.02~0.29, P < 0.000 01) were significantly lower in my⁃ cophenolate mofetil group than those of cyclophosphamide group. There were no significant differences in respiratory tract in⁃ fection rate (RR=0.68, 95%CI=0.41~1.14, P=0.14) and lung infection rate (RR=0.58, 95%CI =0.31~1.08, P=0.09) between the two groups. Conclusion The safety and efficacy are better in the treatment of refractory nephrotic syndrome using myco⁃ phenolate mofetil than that of cyclophosphamide. Related Articles | Metrics

Select

Progress in correlation of long non-coding RNA with breast cancer invasion and metastasis SU Wei, ZHANG Jin 2015, 43 (5):  566-569.  doi: 10.11958/j.issn.0253-9896.2015.05.030 Abstract ( 701 )   PDF (308KB) ( 4557 )   Abstract:Long non-coding RNA(lncRNA) is a group of functional RNA molecules, which is more than 200 nucleotides in length and lacks ability of encoding protein. The current study indicates that the abnormal expression of lncRNA is closely related with breast cancer progression. Some lncRNA takes part in regulating the process of breast cancer invasion and me⁃ tastasis. This article reviews the abnormal expression of lncRNA in breast cancer and the relevance with breast cancer inva⁃ sion and metastasis, which is expected to provide new strategy for breast cancer diagnosis and treatment. Related Articles | Metrics

Select

Research advances of microRNA related to oral cancer LIU Xiaonan,ZHANG Wenchao 2015, 43 (5):  570-572.  doi: 10.11958/j.issn.0253-9896.2015.05.031 Abstract ( 672 )   PDF (277KB) ( 4296 )   Oral cancer is a common cancer of head and neck. Regional recurrence and metastasis are the main course of the poor prognosis. But the exact molecular mechanism is still unknown. Studies have showed that microRNA plays an im⁃ portant role in the tumorigenesis, invasion and metastasis of oral cancer. And it is expected to become a valuable diagnostic marker and therapeutic target. This paper makes a review about the microRNA related to oral cancer. Related Articles | Metrics

Select

Research progress of Nrf2/ARE pathway regulating mechanism YI Xiaofang, TAN Chao 2015, 43 (5):  573-575.  doi: 10.11958/j.issn.0253-9896.2015.05.032 Abstract ( 1489 )   PDF (265KB) ( 8416 )   Abstract: Nuclear factor E2 related factor Nrf2 is a nuclear transcription factors involved in a variety of protein expres⁃ sion. As a center of oxidative stress regulation, it combines with antioxidant components (antioxidant responsive element, ARE) and activates downstream multiple anti-oxidation, anti-inflammatory and detoxifying enzyme protein expression. This signaling pathway is involved in the development of inflammation, tumor and other pathological process. This review de⁃ scribes the basic structure, biological effects and signaling pathways of Nrf2, summarizes the latest progress about mecha⁃ nisms of factors, which are involved in the positive and negative regulations of signal pathway, providing a new target for antiinflammatory, antioxidant, and antitumor biochemical treatment. Based on these, the paper also looks forward to applicating bioinformatics technology and providing better prospects for the development of target intervention. Related Articles | Metrics

Select

Cryotherapy in the treatment for tracheal carinal giant tumor:a case report 2015, 43 (5):  576-576.  doi: 10.11958/j.issn.0253-9896.2015.05.033 Abstract ( 752 )   PDF (607KB) ( 3928 )   Related Articles | Metrics