Abstract: Abstract: Objective To observe the effect of sanfukechuangao acupoint application on changes of interleukin- 18 (IL-18), eosinophils (EOS) and pulmonary pathology in asthma model of guinea pigs, and its underlying mechanism thereof. Methods A total of 60 guinea pigs were randomized into normal control group, asthma model group, acupoint-application 1 h group, acupoint-application 3 h group, acupoint-application 6 h group and dexamethasone group. Asthma model was es⁃ tablished by intraperitoneal injection of 10% ovalbumin (OVA， 1 mL) and forced inhalation of atomized 2% OVA. Skin prep⁃ aration and external application of Chinese medicinal herbs were made on back of guinea pigs, and "Feishu", "Xinshu", "Ge⁃ shu"were selected in acupuncture points). Levels of IL- 18 and EOS were detected by immunohistochemisty method. Re⁃ sults Compared with model group， the levels of IL-18 and EOS were significantly decreased, the pathological changes were significantly reduced in three acupoint- application groups. The effectiveness of acupoint-application 3 h group was the best in three groups. Conclusion Sanfukechuangao acupoint application can influence the levels of IL-18 and EOS effectively, and relieve phlegmonosis injuries in pulmonary tissue of asthma model of guinea pig.

Key words: SanfuKechuangao, Acupoint Application, IL-18, Eos, Asthma, Guinea pig