Abstract: Objective To explore expression levels of SIRT1 mRNA and protein in adipose tissue of obese patients, and its clinical significance in the treatment of obesity. Methods A total of 105 patients in our hospital were selected and divided into obesity group (BMI≥23 kg/m2) and control group (18.5 kg/m2 ≤BMI＜23 kg/m2) according to body mass index (BMI). SIRT1 mRNA expression levels in adipose tissue (collected from peritoneum) were detected by quantitative PCR method in two group. SIRT1 protein expression levels of adipose tissue were detected by enzyme- linked immunosorbent (ELISA) in two groups. The relationships of SIRT1 expression, BMI, total cholesterol (TC), triglyceride (TG), fasting plasma glucose (FPG) and insulin resistance (HOMA- IR) were analyzed between two group. Results SIRT1 mRNA and protein levels were significantly lower in obesity group than those of normal group (P＜0.05). The values of TC, TG, FPG, FINS and HOMA-IRinadiposetissueweresignificantlyhigherinobesitygroupthanthoseofnormalgroup(P＜0.05).Theproteinexpression level of SIRT1 was positively correlated with values of TC, TG, FPG, FINS and HOMA-IR (r=-0.391, -0.941, -0.184, 0.215 and -0.990, P<0.05). Conclusion SIRT1 mRNA and protein expression levels in adipose tissue are significantly reduced in obesity patients than those of control group. There is a close relationship between SIRT1 protein level and obesity index, which has certain guidance for clinical treatment of obesity.

Key words: obesity, subcutaneous fat, abdominal, body mass index, RNA, messenger, dyslipidemias, blood glucose, silent information regulator 1