Abstract: Objective　To study the effect of incomplete embolization of hepatic artery chemoembolization (TACE) on the growth of VX2 liver cancer and Notch2 pathway in rabbits. Methods　The puncture and implantation method was used to establish rabbit VX2 liver cancer model (n=16) . The model rabbits were divided into TACE incomplete embolization group (experimental group, n=8) and the control group (n=8) by random number table. Ultrasound was used to screen the tumor and changes of tumor volume on the day 7, day 14 (on the day of TACE incomplete embolization) and day 21 after tumor implantation. In the experimental group, the model of TACE incomplete embolization of VX2 hepatoma was established under DSA (0.5 mL lipiodol emulsion and 0.5 mL normal saline were injected into the left hepatic artery). In the control group, 1 mL normal saline was injected into the left hepatic artery. The tumor morphology was detected by ordinary CT scan and enhanced CT scan. HE staining was used to observe the morphology of tumor cells. Western blot assay was used to detect the expressions of Notch2, HIF-1α and VEGF proteins. Results　The VX2 hepatoma model was successfully established in 16 rabbits. Rabbits of the experimental group were successfully established VX2 hepatoma TACE incomplete embolism model under DSA. The tumor volume (2.42±0.97) cm3 was significantly lower in the experimental group than that in the control group (6.79 cm3±4.76 cm3, P＜0.05). Ordinary CT scan and enhanced CT scan before and after TACE showed that the tumors in the two groups were consistent with the performance of hepatoma or hepatoma after TACE incomplete embolization. In the control group, a large number of hepatoma cells were seen by HE staining, while in the experimental group, hepatoma cells were necrosis and a small number of hepatoma cells remained around. The expression level of HIF-1α protein was significantly higher in the experimental group than that in the control group (P＜0.05). There was no significant difference in the expressions of Notch2 and VEGF proteins between the two groups. Conclusion　TACE incomplete embolization can inhibit the growth of VX2 hepatoma in rabbits and induce the high expression of HIF-1α protein in tumor hypoxia microenvironment.