The diagnostic value of TIM-3 and GATA-3 levels in maternal blood and amniotic fluid for premature rupture of membranes complicated with intrauterine infection

doi:10.11958/20221201

Abstract

Abstract: Objective To analyze the diagnostic value of T cell immunoglobulin mucin molecule-3 (TIM-3) and GATA binding protein-3 (GATA-3) in maternal blood and amniotic fluid in the diagnosis of premature rupture of membranes (PROM) complicated with intrauterine infection. Methods Eighty-nine patients with PROM were regarded as the research subjects. Among them, 42 patients with intrauterine infection were regarded as the infection group, and 47 patients without intrauterine infection were regarded as the uninfected group. Meantime, 45 normal full-term pregnant women were used as the control group. According to the severity of intrauterine infection, PROM complicated with intrauterine infection patients were divided into the mild group (16 cases), the moderate group (17 cases) and the severe group (9 cases). The mRNA and protein levels of TIM-3, GATA-3 in whole blood and amniotic fluid were detected. The mRNA and protein levels of TIM-3, GATA-3 in whole blood and amniotic fluid were compared between the control group, the uninfected group, the infection group and pregnant women with different degrees of infection. The efficacy of TIM-3 and GATA-3 proteins in serum and amniotic fluid alone and in combination in the diagnosis of intrauterine infection in pregnant women with PROM were analyzed by receiver operating characteristic curve. Results The TIM-3 mRNA and protein levels in whole blood and amniotic fluid of the control group, the uninfected group and the infection group were increased in turn (P<0.05), and GATA-3 mRNA and protein levels decreased in turn (P<0.05). The TIM-3 mRNA and protein levels in whole blood and amniotic fluid of the mild group, the moderate group and the severe group increased in turn (P<0.05), and GATA-3 mRNA and protein levels decreased in turn (P<0.05). The area under curve of serum TIM-3 and GATA-3 protein alone and in combination, and amniotic fluid TIM-3 and GATA-3 protein alone and in combination to diagnose intrauterine infection in pregnant women with PROM were 0.854, 0.813, 0.937, 0.834, 0.850 and 0.922, respectively. Conclusion The maternal blood and amniotic fluid of patients with PROM complicated with intrauterine infection have high expression of TIM-3 and low expression of GATA-3, both of them have certain diagnostic value for intrauterine infection in pregnant women with PROM, and the combined diagnostic efficiency is higher.

Key words: fetal membranes, premature rupture, amniotic fluid, hepatitis A virus cellular receptor 2, maternal blood, T cell immunoglobulin mucin molecule-3, GATA binding protein-3, intrauterine infection

CLC Number:

R714

Figures/Tables 8

References 20

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基因名称	引物序列（5′→3′）	产物大小（bp）
TIM-3	上游：ACCTGAAGTTGGTCATCAAACCA	227
	下游：CCTTGGAAAGGCTGCAGTGA	227
GATA-3	上游：AGGGAGTGTGTGAACTGTGGG	284
	下游：CTTCGCTTGGGCTTAATGAGG	284
GAPDH	上游：GAGTCAACGGATTTGGTCGTT	213
	下游：TTGATTTTGGAGGGATCTCG	213

基因名称	引物序列（5′→3′）	产物大小（bp）
TIM-3	上游：ACCTGAAGTTGGTCATCAAACCA	227
	下游：CCTTGGAAAGGCTGCAGTGA	227
GATA-3	上游：AGGGAGTGTGTGAACTGTGGG	284
	下游：CTTCGCTTGGGCTTAATGAGG	284
GAPDH	上游：GAGTCAACGGATTTGGTCGTT	213
	下游：TTGATTTTGGAGGGATCTCG	213

组别	n	血浆		羊水
组别	n	TIM-3	GATA-3	TIM-3	GATA-3
对照组	45	1.03±0.14	1.05±0.13	1.01±0.10	0.99±0.12
未感染组	47	1.42±0.25^a	0.83±0.15^a	1.47±0.22^a	0.78±0.14^a
感染组	42	1.83±0.31^ab	0.62±0.12^ab	1.86±0.29^ab	0.59±0.13^ab
F		118.620^＊＊	111.190^＊＊	168.942^＊＊	102.437^＊＊

组别	n	血浆		羊水
组别	n	TIM-3	GATA-3	TIM-3	GATA-3
对照组	45	1.03±0.14	1.05±0.13	1.01±0.10	0.99±0.12
未感染组	47	1.42±0.25^a	0.83±0.15^a	1.47±0.22^a	0.78±0.14^a
感染组	42	1.83±0.31^ab	0.62±0.12^ab	1.86±0.29^ab	0.59±0.13^ab
F		118.620^＊＊	111.190^＊＊	168.942^＊＊	102.437^＊＊

组别	n		血清
组别	n		TIM-3蛋白（ng/L）		GATA-3蛋白（μg/L）
对照组	45		214.39±33.76		362.49±37.25
未感染组	47		285.32±38.51^a		298.62±32.21^a
感染组	42		342.77±40.82^ab		258.46±29.04^ab
F			126.482^＊＊		110.019^＊＊
组别		羊水
组别		TIM-3蛋白（ng/L）		GATA-3蛋白（μg/L）
对照组		227.83±35.12		346.72±36.09
未感染组		305.14±39.23^a		287.41±32.85^a
感染组		360.57±42.58^ab		239.53±27.68^ab
F		127.491^＊＊		118.987^＊＊