Tianjin Medical Journal ›› 2023, Vol. 51 ›› Issue (4): 400-403.doi: 10.11958/20221539

• Clinical Research • Previous Articles     Next Articles

Expression and clinical significance of CD163 in hepatic fibrosis with biliary atresia

LIU Zhijie1,2(), ZHENG Qipeng1,2, XU Xiaodan1,2, WANG Qiong1,2, LI Mengdi1,2, ZHANG Cong1,2, WANG Zhiru1,2, ZHAN Jianghua2,()   

  1. 1 Graduate School of Tianjin Medical University, Tianjin 300070, China
    2 Department of General Surgery, Tianjin Children's Hospital
  • Received:2022-09-22 Revised:2022-11-28 Published:2023-04-15 Online:2023-04-20
  • Contact: ZHAN Jianghua E-mail:liuzhijiexj@163.com;zhanjianghuatj@163.com

Abstract:

Objective To investigate the expression of CD163 in children with biliary atresia (BA) and its relationship with liver fibrosis. Methods A total of 30 children with BA (the BA group, 14 patients with liver fibrosis grade Ⅰ-Ⅱ, 16 patients with liver fibrosis grade Ⅲ-Ⅳ) and 10 children with choledochal cyst (the CC group) were collected. Samples of liver tissue and blood were obtained from the 2 groups. The expression levels of CD163 in liver tissue and blood of the two groups were detected by immunohistochemistry and flow cytometry. The expression of sCD163 was detected by enzyme-linked immunosorbent assay (ELISA), and the value of sCD163 in evaluating BA liver fibrosis was analyzed by receiver operating characteristic curve (ROC). Results Immunohistochemical staining and flow cytometry indicated that CD163 expression levels in liver tissue and blood were significantly higher in the BA group than those in the CC group (P<0.01), and which increased with the increased severity of liver fibrosis (P<0.01). The expression of sCD163 also showed the same change. sCD163 predicted BA with an area under the ROC curve of 0.927, a best threshold value of 8.323 μg/L, a sensitivity of 86.67% and a specificity of 100%. Conclusion The expression of CD163 is up-regulated in children with BA, which accelerates the process of liver fibrosis. sCD163 can accurately reflect the severity of liver fibrosis in children with BA.

Key words: biliary atresia, hepatic fibrosis, CD163

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