Effects of taurine on proliferation, apoptosis and migration of human pancreatic cancer cell lines BxPC-3 and PANC-1

doi:10.11958/20221545

Abstract

Abstract:

Objective To investigate the effects of taurine (Tau) on the proliferation, apoptosis and migration of pancreatic cancer ductal cells. Methods BxPC-3 and PANC-1 cells were cultured in vitro and pretreated with different concentrations of Tau (0, 10, 20, 40, 80, 160 mmol/L). Cell counting kit-8 (CCK-8), cell scratch assay and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method were used to observe the effect of Tau on the proliferation, migration and apoptosis of BxPC-3 and PANC-1 cells. Quantitative real-time polymerase chain reaction (qPCR) and Western blot assay were used to detect the effect of Tau on mRNA and protein expression of relevant apoptosis and cell cycle molecules in BxPC-3 and PANC-1 cells. Results Tau significantly inhibited the proliferation and migration activities of BxPC-3 and PANC-1, and Tau could promote the apoptosis of BxPC-3 and PANC-1 cells. Compared with the control group, expression levels of P53, P21 and BAX in BxPC-3 cells treated with Tau showed a significant upward trend, while the expression of PCNA was significantly decreased. The expression levels of Bcl-2, PCNA, CyclinA2, CyclinB1, CyclinE, CDK1, CDK2, CDK4 and CDK6 in PANC-1 cells were significantly lower than those in the normal control group, while the expression levels of P53 and P21 showed an upward trend. Conclusion Tau can inhibit the proliferation and migration activity of BxPC-3 and PANC-1 pancreatic cancer cells, and promote cell apoptosis, possibly by affecting related apoptosis genes and cyclins.

Key words: Taurine, pancreatic neoplasms, tumor suppressor protein p53, cyclin-dependent kinase inhibitor p21, cyclins, cyclin-dependent kinase

CLC Number:

R349.5

Figures/Tables 10

References 19

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基因	引物序列（5′→3′）	产物大小（bp）
β-actin	上游：CATGTACGTTGCTATCCAGGC 下游：CTCCTTAATGTCACGCACGAT	250
p21	上游：GGATGTCCGTCAGAACCCAT 下游：CCCTCCAGTGGTGTCTCGGTG	165
p53	上游：GGACCCTGTCACCGAGACCCC 下游：GGGAGGAGAGTACGTGCACATA	142
CyclinA2	上游：GCCTTTCATTTAGCACTC 下游：TGAAGGTCCATGAGACAA	110
CyclinB1	上游：GGAAACATGAGAGCCATCCT 下游：TTCTGCATGAACCGATCAAT	113
BAX	上游：ACAGCAAAATTAAGGCAGGACTC 下游：TCGAGGACGACTTCAACTATGG	189
BCL-2	上游：CTGGGATGCCTTTGTGGAAC 下游：GGCAGGGCATGTTGACTTCAC	156
CDK1	上游：GGATGTGCTTATGCAGGATTCC 下游：CATGTACTGACCAGGAGGGATAG	100
CDK2	上游：CCAGGAGTTACTTCTATGCCTGA 下游：TTCATCCAGGGGAGGTACAAC	90
CDK4	上游：TCAGCACAGTTCGTGAGGTG 下游：GTCCATCAGCCGGACAACAT	77
CDK6	上游：CCAGATGGCTCTAACCTCAGT 下游：AACTTCCACGAAAAAGAGGCTT	152
CCNE	上游：AAGGAGCGGGACACCATGA 下游：ACGGTCACGTTTGCCTTCC	80
PCNA	上游：ACACTAAGGGCCGAAGATAACG 下游：ACAGCATCTCCAATATGGCTGA	209

基因	引物序列（5′→3′）	产物大小（bp）
β-actin	上游：CATGTACGTTGCTATCCAGGC 下游：CTCCTTAATGTCACGCACGAT	250
p21	上游：GGATGTCCGTCAGAACCCAT 下游：CCCTCCAGTGGTGTCTCGGTG	165
p53	上游：GGACCCTGTCACCGAGACCCC 下游：GGGAGGAGAGTACGTGCACATA	142
CyclinA2	上游：GCCTTTCATTTAGCACTC 下游：TGAAGGTCCATGAGACAA	110
CyclinB1	上游：GGAAACATGAGAGCCATCCT 下游：TTCTGCATGAACCGATCAAT	113
BAX	上游：ACAGCAAAATTAAGGCAGGACTC 下游：TCGAGGACGACTTCAACTATGG	189
BCL-2	上游：CTGGGATGCCTTTGTGGAAC 下游：GGCAGGGCATGTTGACTTCAC	156
CDK1	上游：GGATGTGCTTATGCAGGATTCC 下游：CATGTACTGACCAGGAGGGATAG	100
CDK2	上游：CCAGGAGTTACTTCTATGCCTGA 下游：TTCATCCAGGGGAGGTACAAC	90
CDK4	上游：TCAGCACAGTTCGTGAGGTG 下游：GTCCATCAGCCGGACAACAT	77
CDK6	上游：CCAGATGGCTCTAACCTCAGT 下游：AACTTCCACGAAAAAGAGGCTT	152
CCNE	上游：AAGGAGCGGGACACCATGA 下游：ACGGTCACGTTTGCCTTCC	80
PCNA	上游：ACACTAAGGGCCGAAGATAACG 下游：ACAGCATCTCCAATATGGCTGA	209

组别	p53			p21
对照组	1.00±0.00			1.00±0.00
Tau 40 mmol/L组	3.26±0.49^a			2.71±0.64^a
Tau 80 mmol/L组	10.70±0.51^ab			9.05±0.26^ab
F	460.900^＊＊			342.500^＊＊
组别		BAX	PCNA
对照组		1.00±0.00	1.00±0.00
Tau 40 mmol/L组		2.12±0.28^a	0.69±0.08^a
Tau 80 mmol/L组		3.14±0.28^ab	0.49±0.08^ab
F		67.110^＊＊	45.080^＊＊

组别	p53			p21
对照组	1.00±0.00			1.00±0.00
Tau 40 mmol/L组	3.26±0.49^a			2.71±0.64^a
Tau 80 mmol/L组	10.70±0.51^ab			9.05±0.26^ab
F	460.900^＊＊			342.500^＊＊
组别		BAX	PCNA
对照组		1.00±0.00	1.00±0.00
Tau 40 mmol/L组		2.12±0.28^a	0.69±0.08^a
Tau 80 mmol/L组		3.14±0.28^ab	0.49±0.08^ab
F		67.110^＊＊	45.080^＊＊

组别	p53		p21		Bcl-2		PCNA		CyclinA2		CyclinB1
对照组	1.00±0.00		1.00±0.00		1.00±0.00		1.00±0.00		1.00±0.00		1.00±0.00
Tau 40 mmol/L组	1.31±0.09		1.22±0.10		0.08±0.02^a		0.23±0.09^a		0.11±0.02^a		0.23±0.12^a
Tau 80 mmol/L组	1.80±0.25^ab		1.54±0.17^ab		0.15±0.08^a		0.27±0.01^a		0.07±0.02^a		0.06±0.02^a
F	20.640^＊＊		17.070^＊＊		352.200^＊＊		221.200^＊＊		2 296.000^＊＊		150.400^＊＊
组别		CyclinE		CDK1		CDK2		CDK4		CDK6
对照组		1.00±0.00		1.00±0.00		1.00±0.00		1.00±0.00		1.00±0.00
Tau 40 mmol/L组		0.16±0.05^a		0.06±0.01^a		0.11±0.07^a		0.10±0.02^a		0.33±0.10^a
Tau 80 mmol/L组		0.16±0.05^a		0.03±0.01^ab		0.08±0.07^a		0.13±0.08^a		0.27±0.04^a
F		413.200^＊＊		8 613.00^＊＊		337.800^＊＊		338.900^＊＊		121.700^＊＊