Magu Xujin capsule promotes hip fracture healing in aged rats through SDF-1/CXCR4 signaling pathway

doi:10.11958/20241261

Abstract

Abstract:

Objective To explore the effects of Magu Xujin capsule on hip fracture healing and stromal cell-derived factor-1/CXC chemokine receptor 4 (SDF-1/CXCR4) signaling pathway in elderly hip fracture rats. Methods The hip fracture model of aged rats was established, and successfully modeled rats were randomly separated into the model group, the low-dose Magu Xujin capsule group, the high-dose Magu Xujin capsule group, the high-dose Magu Xujin capsule+pathway inhibitor group (the high-dose Magu Xujin capsule and AMD3100 group), with 12 rats in each group. An additional 12 healthy rats were selected as the control group. X-ray was used to observe the healing of hip fractures in rats, and Garrett score was performed. Enzyme-linked immunosorbent assay (ELISA) was applied to detect serum levels of inflammatory factors related to fracture healing, such as tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10). CT scanning analysis was used to determine bone volume fraction (BV/TV) and bone surface area/volume ratio (BS/BV). Immunohistochemistry was applied to detect the expression of fracture healing factor insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-2 (BMP-2). Western blot assay was applied to detect the expression of SDF-1/CXCR4 signaling pathway related proteins. Results Garrett score, IL-10 level, BV/TV, BS/BV, the IGF-1, BMP-2, SDF-1 and CXCR4 expression were lower in the model group than those in the control group, while TNF-α level was higher (P<0.05). Garrett score, IL-10 level, BV/TV, BS/BV, IGF-1, BMP-2, SDF-1 and CXCR4 expression were higher in the low-dose and high-dose Magu Xujin capsule groups than those in the model group, while TNF-α level was lower (P<0.05). Garrett score, IL-10 level, BV/TV, BS/BV, IGF-1, BMP-2, SDF-1 and CXCR4 expression were lower in the high-dose Magu Xujin capsule+AMD3100 group than those in the high-dose Magu Xujin capsule group, while TNF-α level was higher (P<0.05). Conclusion Magu Xujin capsule can promote hip fracture healing in aged rats, and its mechanism may be related to the activation of SDF-1/CXCR4 signaling pathway.

Key words: hip fractures, fracture healing, chemokine CXCL12, receptors, CXCR6, Magu Xujin capsule

CLC Number:

R683

Figures/Tables 7

References 19

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组别	Garrett评分/分	TNF-α/（ng/L）	IL-10/（ng/L）
对照组	2.75±0.29	5.22±0.54	33.84±3.49
模型组	0.50±0.06^a	30.56±3.17^a	6.71±0.72^a
马骨续筋胶囊低剂量组	1.50±0.16^b	18.34±1.93^b	18.63±1.95^b
马骨续筋胶囊高剂量组	2.58±0.28^bc	6.75±0.71^bc	31.59±3.36^bc
马骨续筋胶囊高剂量+AMD3100组	1.58±0.17^d	17.48±1.85^d	19.42±2.03^d
F	226.950^＊＊	350.894^＊＊	227.478^＊＊

组别	Garrett评分/分	TNF-α/（ng/L）	IL-10/（ng/L）
对照组	2.75±0.29	5.22±0.54	33.84±3.49
模型组	0.50±0.06^a	30.56±3.17^a	6.71±0.72^a
马骨续筋胶囊低剂量组	1.50±0.16^b	18.34±1.93^b	18.63±1.95^b
马骨续筋胶囊高剂量组	2.58±0.28^bc	6.75±0.71^bc	31.59±3.36^bc
马骨续筋胶囊高剂量+AMD3100组	1.58±0.17^d	17.48±1.85^d	19.42±2.03^d
F	226.950^＊＊	350.894^＊＊	227.478^＊＊

组别	BV/TV	BS/BV
对照组	0.20±0.03	3.28±0.35
模型组	0.05±0.01^a	1.02±0.12^a
马骨续筋胶囊低剂量组	0.12±0.02^b	2.05±0.21^b
马骨续筋胶囊高剂量组	0.19±0.03^bc	3.14±0.33^bc
马骨续筋胶囊高剂量+AMD3100组	0.13±0.02^d	2.19±0.24^d
F	40.778^＊＊	72.788^＊＊

组别	BV/TV	BS/BV
对照组	0.20±0.03	3.28±0.35
模型组	0.05±0.01^a	1.02±0.12^a
马骨续筋胶囊低剂量组	0.12±0.02^b	2.05±0.21^b
马骨续筋胶囊高剂量组	0.19±0.03^bc	3.14±0.33^bc
马骨续筋胶囊高剂量+AMD3100组	0.13±0.02^d	2.19±0.24^d
F	40.778^＊＊	72.788^＊＊

组别	IGF-1	BMP-2
对照组	0.71±0.08	0.56±0.06
模型组	0.26±0.03^a	0.11±0.02^a
马骨续筋胶囊低剂量组	0.44±0.05^b	0.30±0.03^b
马骨续筋胶囊高剂量组	0.65±0.07^bc	0.52±0.06^bc
马骨续筋胶囊高剂量+AMD3100组	0.48±0.05^d	0.31±0.04^d
F	55.762^＊＊	99.653^＊＊