Experimental study of vascular extracellular matrix gel promoting the differentiation of bone marrow CD34+ progenitor cells into endothelial cells

doi:10.11958/20221138

Abstract

CLC Number:

R331.3

Figures/Tables 9

Fig.1 A patient’s aortic tissue (scale bar=1 cm)

Fig.2 HE and DAPI staining of human aortic tissue (×40)

Fig.3 Purity of CD34+ progenitor cells

Fig.4 Growth of CD34+ progenitor cells on FN and ECM gel (×20)

Fig.5 mRNA expression of CD31, eNOS and VWF in CD34+ progenitor cells in two groups

Tab.1 Comparison of mRNA expression levels of CD31, eNOS and VWF between the two groups

组别	CD31	eNOS	VWF
FN组	0.858±0.044	0.462±0.137	0.634±0.135
ECM组	0.992±0.077	0.630±0.191	0.745±0.148
t	4.522^＊	5.363^＊	14.520^＊＊

Fig.6 The percentage of CD34+ progenitor cells with positive differentiation-related markers in two groups

Fig.7 Phagocytosis for DiI-ACLDL by CD34+ progenitor cells (×40)

Tab.2 Comparison of levels of secreted angiogenic factors between the two groups

组别	Angiogenin	FGF-7	GM-CSF	Leptin	PDGF-BB
FN组	184.7±24.2	425.7±34.3	104.3±22.0	548.7±74.2	634.7±85.1
ECM组	473.3±46.1	767.3±45.0	192.0±29.5	848.7±136.5	1 009.2±199.1
t	10.810^＊＊	12.090^＊＊	16.730^＊	8.294^＊	5.662^＊

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