The effect of Thioridazine on proliferation and apoptosis of pancreatic cancer cells

doi:10.11958/20220932

Figures/Tables 11

Fig.1 The effect of TDZ on clone formation of pancreatic cancer SW1990 cells

Tab.1 Effects of TDZ on proliferation inhibition rate and clone formation number of pancreatic cancer SW1990 cells

组别	增殖抑制率（%）	细胞克隆形成数（个/视野）
对照组	0.00±0.00	81.15±7.01
TDZ低剂量组	17.71±1.64^a	63.82±6.05^a
TDZ中剂量组	36.71±3.04^ab	44.86±5.08^ab
TDZ高剂量组	59.44±5.18^abc	28.91±2.83^abc
F	604.698^＊＊	155.044^＊＊

Fig.2 The effect of TDZ on apoptosis of pancreatic cancer cell line SW1990

Fig.3 Expression of apoptosis-related proteins in each group

Tab.2 Effects of TDZ on apoptosis rate and expression of apoptosis-related proteins of pancreatic cancer SW1990 cells

组别	凋亡率（%）	cleaved-caspase3 蛋白	cleaved-caspase9 蛋白
对照组	6.04±0.51	0.23±0.02	0.16±0.02
TDZ低剂量组	11.96±1.07^a	0.36±0.03^a	0.29±0.02^a
TDZ中剂量组	18.96±1.58^ab	0.53±0.04^ab	0.44±0.04^ab
TDZ高剂量组	27.31±2.07^abc	0.67±0.05^abc	0.59±0.06^abc
F	369.665^＊＊	247.278^＊＊	155.700^＊＊

Fig.4 Effects of interfering LINC00470 expression on clone formation and apoptosis of pancreatic cancer cell line SW1990

Fig.5 Effects of interfering LINC00470 expression on the expression of apoptosis-related proteins in pancreatic cancer SW1990 cells

Tab.3 Effects of LINC00470 expression interference on proliferation inhibition rate, clone formation number, apoptosis rate and apoptosis-related protein expression levels of pancreatic cancer SW1990 cells

组别	LINC00470	抑制率（%）	细胞克隆形成数（个/视野）	凋亡率（%）	cleaved-caspase3蛋白	cleaved-caspase9蛋白
si-NC组	1.00±0.00	6.09±0.57	82.49±7.98	7.15±0.65	0.22±0.02	0.14±0.02
si-LINC00470组	0.31±0.03	38.98±4.52	36.71±3.21	21.83±2.14	0.61±0.05	0.52±0.04
t	69.000^＊＊	21.658^＊＊	15.967^＊＊	19.691^＊＊	21.726^＊＊	25.491^＊＊

Fig.6 Overexpression of LINC00470 reversed the effect of TDZ on clone formation and apoptosis of pancreatic cancer SW1990 cells

Fig.7 Overexpression of LINC00470 reversed the effect of TDZ on the expression of apoptosis-related proteins in pancreatic cancer SW1990 cells

Tab.4 LINC00470过表达逆转了TDZ对胰腺癌SW1990细胞增殖抑制率、克隆形成数、凋亡和凋亡相关蛋白表达的作用 (n=9, $\bar{x}$±s)

组别	LINC00470	抑制率（%）	细胞克隆形成数（个/视野）	凋亡率（%）	cleaved-caspase3蛋白	cleaved-caspase9蛋白
TDZ+pcDNA组	1.00±0.00	60.84±5.89	27.24±3.58	29.03±2.39	0.69±0.05	0.56±0.04
TDZ+pcDNA-LINC00470组	3.44±0.23	21.63±2.34	69.51±6.33	12.96±1.07	0.33±0.03	0.25±0.03
t	31.826^＊＊	18.560^＊＊	17.438^＊＊	18.411^＊＊	18.522^＊＊	18.600^＊＊

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