Expression levels and clinical significance of Galectin-3, IL-1β and IL-9 in peripheral blood of children with refractory mycoplasma pneumoniae pneumonia

doi:10.11958/20250076

Abstract

Abstract:

Objective To investigate the expression levels and clinical significance of Galectin-3, interleukin (IL)-1β and IL-9 in peripheral blood of children with refractory mycoplasma pneumoniae pneumonia (RMPP). Methods A total of 132 hospitalized children diagnosed with RMPP were selected as the RMPP group, and another 128 children with general MPP (GMPP) in the same period were selected as the GMPP group. The clinical data of C-reactive protein (CRP), tumor necrosis factor-α (TNF-α) and procalcitonin (PCT) in pediatric patients were collected. The expression levels of Galectin-3, IL-1β and IL-9 were detected by enzyme-linked immunosorbent assay (ELISA). The correlation between Galectin-3, IL-1β and IL-9 levels in peripheral blood of RMPP children and clinical factors were analyzed by Pearson or Spearman methods. Multivariate Logistic regression analysis was used to analyze risk factors of GMPP progression to RMPP. The predictive value of peripheral blood Galectin-3, IL-1β and IL-9 levels to RMPP was analyzed by receiver operating characteristic (ROC) curve. Results The heat course, CRP, TNF-α, PCT, Galectin-3, IL-1β, IL-9 and the proportion of extrapulmonary complications were higher in the RMPP group than those in the GMPP group (P<0.01). The correlation analysis results showed that Galectin-3, IL-1β and IL-9 levels were positively correlated with heat course, extrapulmonary complications, CRP, TNF-α and PCT (P<0.01). Logistic regression analysis showed that the increased levels of Galectin-3, IL-1β and IL-9 were independent risk factors for GMPP progression to RMPP (P<0.05). The area under the curve (AUC) of peripheral blood Galectin-3, IL-1β, IL-9 and their combined prediction of GMPP progression to RMPP were 0.852, 0.813, 0.812 and 0.949, respectively, and the combined prediction showed the highest efficacy (P<0.01). Conclusion The levels of Galectin-3, IL-1β and IL-9 in the peripheral blood of children with RMPP are abnormally elevated, and their combined detection can be used as an important indicator for evaluating the progression of GMPP to RMPP.

Key words: pneumonia, mycoplasma, galectin 3, interleukin-1beta, interleukin-9

CLC Number:

R725

Figures/Tables 6

References 21

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组别		n		男性	年龄/岁		住院时间/d		热程/d
GMPP组		128		72（56.25）	7.25±2.30		7.50±1.85		8.05±2.15
RMPP组		132		76（57.58）	7.40±2.42		7.95±2.25		11.23±2.30
t或χ²				0.047	0.512		1.759		11.509^＊＊
组别	喘息			胸腔积液		胸膜增厚	肺外并发症		肺部湿啰音
GMPP组	21（16.41）			22（17.19）		15（11.72）	8（6.25）		23（17.97）
RMPP组	28（21.21）			25（18.94）		18（13.64）	22（16.67）		29（21.97）
χ²	0.981			0.135		0.216	6.908^＊＊		0.650
组别			CRP/（mg/L）			TNF-α/（μg/L）		PCT/（μg/L）
GMPP组			14.75±3.30			2.40±0.64		0.12±0.03
RMPP组			18.48±4.09			3.12±0.92		0.15±0.04
t			8.078^＊＊			7.305^＊＊		6.825^＊＊

组别		n		男性	年龄/岁		住院时间/d		热程/d
GMPP组		128		72（56.25）	7.25±2.30		7.50±1.85		8.05±2.15
RMPP组		132		76（57.58）	7.40±2.42		7.95±2.25		11.23±2.30
t或χ²				0.047	0.512		1.759		11.509^＊＊
组别	喘息			胸腔积液		胸膜增厚	肺外并发症		肺部湿啰音
GMPP组	21（16.41）			22（17.19）		15（11.72）	8（6.25）		23（17.97）
RMPP组	28（21.21）			25（18.94）		18（13.64）	22（16.67）		29（21.97）
χ²	0.981			0.135		0.216	6.908^＊＊		0.650
组别			CRP/（mg/L）			TNF-α/（μg/L）		PCT/（μg/L）
GMPP组			14.75±3.30			2.40±0.64		0.12±0.03
RMPP组			18.48±4.09			3.12±0.92		0.15±0.04
t			8.078^＊＊			7.305^＊＊		6.825^＊＊

组别	n	Galectin-3	IL-1β	IL-9
GMPP组	128	5.51±1.37	5.86±1.41	57.32±12.56
RMPP组	132	7.47±1.92	8.10±2.02	75.84±18.20
t		9.450^＊＊	10.339^＊＊	9.522^＊＊

组别	n	Galectin-3	IL-1β	IL-9
GMPP组	128	5.51±1.37	5.86±1.41	57.32±12.56
RMPP组	132	7.47±1.92	8.10±2.02	75.84±18.20
t		9.450^＊＊	10.339^＊＊	9.522^＊＊

变量	Galectin-3	IL-1β	IL-9
热程	0.507^＊＊	0.494^＊＊	0.497^＊＊
肺外并发症	0.512^＊＊	0.512^＊＊	0.516^＊＊
CRP	0.526^＊＊	0.548^＊＊	0.539^＊＊
TNF-α	0.538^＊＊	0.536^＊＊	0.509^＊＊
PCT	0.517^＊＊	0.527^＊＊	0.521^＊＊