Abstract: Objective   To establish a model of mouse continuing endotoxemia thrombocytopenia (TCP) induced by lipopolysaccharide (LPS) and observe the change of mean platelet volume (MPV) and thrombopoietin (TPO) in this model. Methods   C57/BL mice were randomly divided into four groups, group of LPS 5mg/kg low dose of continue injection (LL-c group), group of LPS 5mg/kg low dose of single injection (LL group), group of LPS 50mg/kg high dose of single injection (HL group), group of physiological saline injection (C group). The blood platelet count(PLT) and MPV were detected before injection as base lines,and then observe variation of PLT ,TPO ,MPV and mortality. Results   The mouse PLT was significantly lower in LL-c group among seven days than basic line (P<0.01),which was continued in the base by about 30%. The PLT were low and then high in HL and LL group. The MPV was continued significantly higher in LL-c group than basic line (P<0.01),which was augmentation and then diminution in HL group and LL group.The TPO level of LL-c group in the seventh day was higher than C group,that has significance(P<0.05).The mortality of LL-c group ,LL group and HL group were 25%(5只)、0%(0只)、70%(14只). Conclusion   The model of continuing endotoxemia TCP can be induced by consecutive low dose LPS intraperitoneal injection,the mechanism and therapy of which was futher to study.

Key words: lipopolysaccharides, endotoxemia, thrombocytopenia, thrombopoietin, mice, inbred C57BL, mean platelet volume