Abstract: Objective  To investigate the effect of nourishing kidney and activating blood recipe of traditional Chinese medicine on the expression of Nephrin in renal tissue of mice with adriamycin nephropathy（ADRN） induced by adriamycin（ADR） Methods  The focal segmental glomerulosclerosis( FSGS) change of nephropathy model was induced by injection of adriamycin into the tail vein．After 1 week，Twenty four male rats were randomly divided into normal control group，ADR model group and treatment group.The level of body weight，right renal weight and urinary albumin creatinine ratio (UACR) were measured，Furthermore, the morphological changes of renal tissue were observed under microscope.The mRNA and protein expressions of Nephrin in renal tissues were determined by RT-PCR and immunofluorescence respectively. Results  There was a significantly higher level of body weight and lower level of UACR in normal control group and treatment group than that of ADR model group (P < 0.05). There were intensy expression of Nephrin in glomerular mesangial region in normal control group and treatment group compared with in ADR model group.There were significantly higher expressions of Nephrin mRNA in those of normal control group and treatment group than in ADR model group .(P <0.05) There were no significant differences in Nephrin mRNA between normal control group and treatment group( P>0.05)Conclusion  The method of nourishing kidney and activating blood recipe of traditional Chinese medicine attenuates Nephrin decrease in FSGS change nephropathy model which is closely related with the reduction of proteinuria.

Key words: reinforcing kidney, activating blood, Epirubicin, glomerulosclerosis, focal segmental, podocytes, Disease Models, 动物