Abstract: Objective:To find the expressive proteomics changes in experimental congenital hypothyroidism neonatal rat cerebral cortex，, which provide valuable clues to clarify the pathogenesis of CH-induced brain developmental disorders. Methods:Neonatal hypothyroidism rat was induced by administation of propylthiouracil （PTU） solution to the dams by gavage（1%PTU，2.5ml/d） on embryonic day 13.Cerebral cortex were collected from the control and hypothyroidism neonatal rats.2-DE analysed protein expression diversities between the euthyroid and hypothyroidism neonatal rat cerebral cortex.RIA analysed serum FT3,FT4 level of each groups. Results:Compared with control group,body weight and serum FT3,FT4 level in CH neonatal rat were lower.Stable 2-DE maps of normal and CH neonatal rat were constantly obtained.Maps were analyzed by software.Eight differential protein spot were found,three differential protein spot were highly expressed in CH group,two differential protein spot were highly expressed in control group,three protein spot present only in CH group.Conclusions:Differential protein expression in CH neonatal rat cerebral may play critical roles in CH.

Key words: congenital hypothyroidism, rat, cerebral cortex, 2-DE