Abstract: Objective To observe the expression of ICAM-1 in the brain of rats with Hhcy or after the intervention of folic acid, Vitamins B12 and Vitamins B6. Methods 40 healthy male Sprague-Dawley rats were divided into 4 groups as:normal group, Hhcy group,treat group, prevent group by the way of different foods and different interventions.The plasma total homocysteine (tHcy) levels of rats were assayed by high-performance liquid chromatography method(HPLC) in different time; At the end of 12th week ,the expression of intercellular adhesion molecule-1 (ICAM-1) were detected by immunohistochemical method in the cerebral vessels of rats. Results At the end of 4th week, the Hhcy model were induced successfully; At the end of 12th week ,there were no statistical difference between the treat group,prevent group and normal group; there were significantly statistical differences between the number of masculine cerebral vessels in the 4 groups(P＜0.05), the sequence is Hhcy group（3.23±0.58）＞treat group（1.54±0.36）＞prevent group（0.98±0.23）＞normal group（0.35±0.31）. Conclusions The expression of ICAM-1 were increased in the cerebral vessels of rats with Hhcy.The intervention of B Vitamins could decrease.

Key words: hyperhomocystinemia, intervention, intercellular adhesion molecule-1, B Vitamins