Tianjin Med J ›› 2015, Vol. 43 ›› Issue (12): 1413-1416.doi: 10.11958/j.issn.0253-9896.2015.12.018

• Clinical Study • Previous Articles     Next Articles

The expression of OCT4 and P27kip1 in renal hyaline cell carcinoma and their correlations

GUAN Youliang1, ZANG Dongyu2, WANG Dan3△   

  1. 1 Department of Urinary Surgery of the First Affiliated Hospital, Liaoning Medical College, Jinzhou 121001,China; 2 Department of Thoraxes Surgery of the Third Affiliated Hospital, Liaoning Medical College; 3 Department of Histology and Embryology, Liaoning Medical College
  • Received:2015-03-16 Revised:2015-06-11 Published:2015-12-15 Online:2015-12-11
  • Contact: △Corresponding Author E-mail:595680903@qq.com E-mail:wanganan1997@sina.com

Abstract: Objective To investigate the expressions of OCT4 and P27kip1 and their correlations in renal hyaline cell carcinoma. Methods A total of 24 samples of renal hyaline cell carcinoma were detected by immunohistochemistry. The rate of cells with positive labelling of OCT4 and P27kip1 and their intra cellular distribution were observed in renal hyaline cell carcinoma. The expression levels of OCT4 and P27kip1 were compared between different gender, age (<60y and ≥60y) and cancer cell metastasis groups. Results The rates of cells with positive OCT4 and P27kip1 expressions were 66.7% and 75% in renal hyaline cell carcinoma respectively. The ratio of cells with high, middle and low expression of OCT4 were 33.3%, 20.5% and 12.5% in renal hyaline cell carcinoma tissues. OCT4 was mainly expressed in cytoplasm and nuclei with a few was expressed in the cell membrane. The ratio of cells with high, middle and low expression of P27kip1 were 12.5%, 25% and 37.5% in renal hyaline cell carcinoma tissues respectively. The positive staining of P27kip1 was in the cytoplasm and cell membrane, and a small number of nuclei were expressed. There were no significant differences in OCT4 and P27kip1 between different age and gender groups. There were significant differences in OCT4 and P27kip1 expressions between patients with metastasis and patients without metastasis (P < 0.05). A negative correlation was found between OCT4 and P27kip1 expres⁃ sions in renal hyaline cell carcinoma (rs=-0.408, P < 0.05). Conclusion The expression of OCT4 is negatively correlated with the expression of P27kip1. Inhibiting expressions of OCT4 or P27kip1, especially knocking down P27kip1 in cytoplasm can be used as a new diagnosis and treatment of renal cancer gene therapy.

Key words: gene therapy, renal clear cell carcinoma, OCT4, P27kip1, immunohistochemistry