Abstract: To explore neuroprotective effects of blonanserin through PC12 cells injury model induced by H2O2. METHODS Used PC12 cells treated with H2O2 as oxidative stress damage cell model. PC12 cells were divided into 4 groups: control group(C group), H2O2-treated group(H group), blonanserin treated group(B group) and positive control group(vitamin E- pretreated, E group). The viability of PC12 cells injured by H2O2 was determined by MTT assay. Used hoechst33258 stained to detect the cell apoptosis and used TUNEL assay to detect the apoptotic rate. The SOD viability and MDA levels were detecded by biochemical methods. RESULTS Comparing with the C group, the apoptotic rate and MDA levels were increased in group H (P＜0.05) , while the cell viability and the SOD viability were decreased obviously(P＜0.05). Compared with H group, the cell viability, SOD viability were significantly increased(p＜0.05), while the MDA levels and apoptotic rate were decreased(P＜0.05). CONCLUSIONS The appropriate concentration of blonanserin showed neuroprotective effect on H2O2-induced PC cells injured.

Key words: trauma, nervous system, apoptosis, PC12 cell, neuroprotective, Blonanserin, SOD, MDA