Tianjin Medical Journal

Tianjin Med J ›› 2016, Vol. 44 ›› Issue (3): 336-341.doi: 10.11958/20150331

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Study on the relationship between expression of miRNAs and receptors of epidermal growth factor in lung adenocarcinoma of non-smoking female

WANG Fumin1, 2, ZHANG Huan1, XU Fangxiu1, QU Jinli1, KONG Jinyu1, QIAN Biyun   

  1. 1 Department of Epidemiology and Biostatistics, Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center of Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China; 2 Graduate School of Tianjin Medical University； 3 Public Health, Shanghai Jiao Tong University School; 4 Hongqiao International Institute of Medicine,Shanghai Jiao Tong University School of Medicine
  • Received:2015-11-19 Revised:2015-12-07 Published:2016-03-15 Online:2016-03-15
  • Contact: QIAN Biyun E-mail:wangfumin57@163.com

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Abstract: Objective To determine association between the expression of miR-155, miR-16, miR-25 and miR-133 and epidermal growth factor receptor (EGFR) in lung adenocarcinoma (ADC) samples of non-smoking female, and their clini⁃ cal significance thereof. Methods According to EGFR types, 112 non-smoking female ADC patients were divided into two groups: EGFR wild (n=51) and EGFR mutant (n=61) groups. Expression levels of four miRNAs were detected by real time quantitative PCR. T-test was used to compare differences of four miRNAs expression in two groups. ADC patients were divid⁃ ed into high miRNA expression group and low expression group according to the mean value of miRNAs expression. Logrank test was used to compare the survival difference between the two subgroups. Cox proportional hazards regression analy⁃ sis was performed to determine the association between different ages (≤60， >60), EGFR types, miRNAs expression and over⁃ all survival. Results The expression of miR-25 was significantly higher in EGFR mutant group than that in EGFR wild group (P < 0.05). There were no significant differences in the survival rates of patients with different ages, EGFR types, and the expressions of miR-155, miR-16 and miR-133a. Patients with the lower expression of miR-25 had the higher survival time than patients with the higher expression of miR-25 (P < 0.05). In EGFR mutation group, there was no relationship be⁃ tween four miRNAs and the prognosis of ADC (P > 0.05). In EGFR wild group, the lower expression of miR-16, miR-25 or miR-133a was associated with longer survival in ADC (P < 0.05). Cox proportional hazards regression analysis revealed that the high expression of miR-25 was an independent risk factor for non-smoking female patients with ADC (P < 0.05). Con⁃ clusion miR-25 is an independent prognostic indicator for non-smoking female patients with ADC. High expression of miR-25 is found in non-smoking female patients with ADC, particularly in patients with EGFR wild-type, which may sug⁃ gest a poor prognosis.

Key words: receptor, epidermal growth factor, moking, sex factors, gene expression, lung adenocarcinoma, miR-155, miR-16, miR-25, miR-133a