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15 December 2017, Volume 45 Issue 12 Previous Issue    Next Issue

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The effect of microRNA-145 on immune-inflammatory response of foam cells by targeting CD40 LIU En-zhao△, LU Li-ping, LIU Yun-ling, LIANG Xue, LI Guang-ping 2017, 45 (12):  1233-1236.  doi: 10.11958/20170738 Abstract ( 749 )   PDF (649KB) ( 3583 )   Objective To investigate the effect of miRNA-145 (miR-145) on immuno-inflammatory reaction of foam cells by targeting CD40. Methods Mouse macrophage cell line RAW 264.7 cells cultured in vitro were randomly divided into model group (non-transfected), miR-145 mimics group (transfected miR-145 mimics), miR-145 inhibitor group (transfected miR-145 inhibitor) and silencing CD40 sequence group (transfected siCD40). Then oxidized low density lipoprotein (ox-LDL) was used to stimulate for 24 h to establish immune inflammatory damage cell model. Quantitative realtime polymerase chain reaction (RT-qPCR) and Western blot assay were used to detect the levels of CD40 mRNA and protein of each group. ELISA was used to detect the levels of inflammatory factors interleukin (IL) - 1, IL-6 and tumor necrosis factor (TNF) - α in cell supernatant. Results Compared with model group, the levels of CD40 mRNA, CD40 protein and IL-1, IL-6, TNF-α were all significantly decreased in miR-145 mimics group (P < 0.01). After transfected with miR-145 inhibitor, the above indexes were all significantly increased than those of model group and miR-145 mimics group (P < 0.01). After transfected with CD40 siRNA, the levels of CD40 mRNA, CD40 protein and IL-1, IL-6, TNF-α were all obviously decreased compared with those of miR-145 inhibitor group (P < 0.01). Conclusion MiR-145 can regulate the immune inflammatory process of foam cells through the target gene CD40, inhibit the activation of CD40/CD40L signaling pathway and inhibit inflammatory response. Related Articles | Metrics

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Effects of FOSL1 on cell proliferation, cell invasiveness and the methylation of PRDM10 gene in breast cancer cell line MDA-MB-231 ZHANG Xiao-yu1, KANG Xiao-ning2, LIU Wei1, LIU Zhi-ming1, WANG Peng1, WANG Zun-yi1△ 2017, 45 (12):  1237-1241.  doi: 10.11958/20170819 Abstract ( 834 )   PDF (733KB) ( 3744 )   Objective To construct the silencing vector of FOS like antigen 1 (FOSL1) gene, and study the effects of FOSL1 on cell proliferation, cell invasiveness and the methylation level of PRDM10 gene in breast cancer cell line MDAMB-231. Methods The FOSL1 silencing vector of gene pLVX-shRNA-FOSL1-shRNA was purchased. The FOSL1 silencing vector and the empty vector were separately transfected into MDA-MB-231, which were regarded as transfection group and empty group, respectively. Untransfected MDA-MB-231 was used as control group. FOSL1 was verified by PCR in MDA-MB-231. The cell proliferation ability and cell invasion ability of MDA-MB-231 were detected by MTT and Transwell assay, respectively. MSP was used to detect the methylation status of PRDM10 gene. The mRNA and protein expression levels of PRDM10 gene were detected by Q-PCR and Western-blot assay. Results MTT results showed that the optical density (OD) values were significantly lower in transfection group compared with those of control group at 24 h, 48 h and 72 h (all P<0.05), and the same as those compared with empty group at 48 h and 72 h (both P<0.05). Compared with empty group and control group, Transwell assays showed that the cell invasive abilities of MDA-MB-231 were decreased in transfection group (both P<0.05), and MSP assay showed that the methylation of PRDM10 gene was decreased in MDA-MB- 231, and Q-PCR and Western-blot tests showed that the expressions of PRDM10 gene were increased in mRNA level and in protein level. Conclusion Silencing of FOSL1 gene inhibits the proliferation and invasion of MDA-MB-231 cells, which might be related to the demethylation of PRDM10 gene in the cells. Related Articles | Metrics

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Effects of FOXO3a on regulating mitophagy in hepatic ischemia reperfusion injury SONG Hu1, ZHANG Jian-jun2△, WANG Zhen2, DU Chen-yang1, ZHENG Hong2, SHEN Zhong-yang2 2017, 45 (12):  1242-1247.  doi: 10.11958/20170865 Abstract ( 890 )   PDF (1438KB) ( 3749 )   Objective To investigate the effect of transcription factor FOXO3a on mitophagy in hepatic ischemiareperfusion injury in mice. Methods A total of 30 male C57BL/6 mice were randomly divided into Sham operation group (Sham) and ischemia reperfusion (IR) 2 h, 6 h, 12 h and 24 h groups, 6 mice in each group. The mouse model of liver ischemia -reperfusion injury was established. Blood biochemical methods were used to detect changes of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). HE staining and TUNEL were used to observe the damages of liver tissue and apoptosis. Western blot assay and qRT-PCR were used to detect the expressions of transcription factor FOXO3a, mitochondrial autophagy-related protein Nix protein and its mRNA expression in each group. Mouse liver AML12 cells were treated with FOXO3a and Nix interfering RNA, and the model was established for 6 h after hypoxia for 1.5 h.These cells were divided into siRNA-NC group, FOXO3a siRNA group and Nix siRNA group. MTT assay was used to detect the viability of cells in each group. The number and distribution of autophagy in each group were observed by confocal microscopy. The expressions of FOXO3a, Nix, microtubule-associated protein LC3, apoptotic protein P62 and Caspase-3 were detected by Western blot assay. Results The levels of ALT and AST in all groups of IR were reduced, and reached the peak value at 6 h (P＜0.05). HE and TUNEL results showed that liver injury and apoptosis were the most serious at 6 h after reperfusion. The expression of FOXO3a and Nix was higher in IR group than that in the Sham group, and the expression level of FOXO3a mRNA was the highest at 12 h after reperfusion, the expression of Nix mRNA was the highest at 6 h after reperfusion (P＜0.05). Western blot assay showed the highest expression of FOXO3a in the reperfusion of 12 h, and the highest expression levels of Nix, Caspase-3 and LC3Ⅱin reperfusion 6 h. After interfering with the expression of FOXO3a, MTT showed a marked reduction in cell survival (P＜0.05), Western blot assay showed that the expression level of FOXO3a was significantly higher in siRNA-NC group than that in FOXO3a siRNA group, and the expression levels of Nix, Caspase-3 and LC3Ⅱwere significantly lower than those of FOXO3a siRNA group. Confocal microscopy showed that the number and distribution of autophagosomes were significantly lower in siRNA-NC group than those in FOXO3a siRNA group. After interfering with the expression of Nix, MTT showed a marked increase in cell survival (P＜0.05), Western blot assay showed that the expression levels of Nix, P62 and LC3Ⅱ were significantly higher in siRNA-NC group than those in Nix siRNA group. Conclusion FOXO3a can reduce the hepatic ischemia-reperfusion injury in mice, which may be related to the FOXO3a inhibition for liver cell mitophagy and apoptosis. Related Articles | Metrics

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Efficacy of mild hypothermia therapy on pulmonary function in ARDS dogs CUI Song1, LU Cheng-quan2， HAN Ye-ye1, KANG Zhi-jie3, LIU Yin-hui4 2017, 45 (12):  1248-1252.  doi: 10.11958/20170621 Abstract ( 772 )   PDF (651KB) ( 3689 )   Objective To investigate the efficacy of mild hypothermia therapy on pulmonary function in acute respiratory distress syndrome (ARDS) dogs. Methods A total of 25 healthy dogs were included and randomly divided into two groups, mild hypothermia treatment group (experimental group, n=12) and normothermia treatment group (control group, n=13). The E. coli was pumped continuously into the canine femoral vein by micro pump to construct the septic shock model in two groups. The hypothermia experimental group was treated with hypothermia (33 ℃±1 ℃), and the control group was observed at room temperature. The pulmonary hemodynamic parameters and respiratory mechanics parameters were supervised by PICCO and respirator respectively at 0, 24 and 48 h during the ARDS progress. Moreover, chest X-ray and lung tissue biopsy were taken to confirm the diagnosis of ARDS after 72 h. Results Up to 72 h, ARDS was found in the experimental group (n=4) and the control group (n=7) respectively. The oxygenation index (OI), partial pressure of oxygen [p(O2)] and pulmonary static compliance (Cst) decreased gradually with the extension of time in two groups. On the contrary, the external venous lung water index (EVLWI), pulmonary vascular permeability index (PVPI) and airway resistance (Raw) increased gradually (P < 0.05). However, all the parameters were significantly better in mild hypothermia group than those of the normothermia group. Conclusion Mild hypothermia therapy can improve the pulmonary function and reduce the severity of ARDS in septic shock dogs. Related Articles | Metrics

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Effects of kangnao liquid on the expressions of LC3 and Beclin 1 in hippocampus after cerebral ischemia-reperfusion in rats SHI Hui-juan1, LI Fan1, XUE Qian2, ZOU Yu-an2△, TIAN Li-xia1, CHANG Qing2, QIN Li-peng1, YANG Hui-xin1, MA Qiang1 2017, 45 (12):  1253-1256.  doi: 10.11958/20170491 Abstract ( 788 )   PDF (1212KB) ( 3523 )   Objective To observe the effect of kangnao liquid on the expressions of LC3 and Beclin 1 in hippocampus after cerebral ischemia-reperfusion in rats, and to discuss the mechanism. Methods A total of 48 male SD rats were randomly divided into four groups: sham-operation group, model group, kangnao liqiud group [14.30 g/(kg·d)] and edaravone group [10.00 mg/(kg·d)], 12 rats in each group. The rats in kangnao liqiud group and edaravone group were administrated by intragastric administration. The rats in sham-operation group and model group were administrated with equal volume of normal saline. After treating for 7 days, except for the sham-operation group, the middle cerebral artery occlusion (MCAO) model was made by suture method in the other three groups. After 2 h of ischemia, the rats were reperfused for 24 h. Six rats in each group were randomly selected for observing the neurological function. TTC staining was used to observe the morphology of the brain tissue and calculate the percentage of infarction. The morphology of the cerebral cortex and hippocampus was observed by HE staining in the remaining animals, and the expressions of LC3 and Beclin 1 in the hippocampus were detected by immunohistochemical staining. Results Compared with the sham-operation group, model group showed an obvious neurological deficit, and the percentage of cerebral infarction increased significantly. At the same time, nerve cells of cerebral cortex and hippocampus revealed degeneration and necrosis, stroma edema, and the expressions of LC3 and Beclin 1 in hippocampus significantly increased (P＜0.05). Compared with the model group, the neurological deficit symptoms and the percentage of cerebral infarction significantly reduced, changes of neuron morphology were lighter, and the expressions of LC3 and Beclin 1 decreased significantly in kangnao liqiud group and edaravone group (P＜0.05). Conclusion Kangnao liquid shows protective effects on cerebral ischemia-reperfusion, which may be related with the decreased expressions of LC3 and Beclin 1 in hippocampus after cerebral ischemia-reperfusion in rats. Related Articles | Metrics

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Clinical observation of Hybrid therapy in repairing muscular ventricular septal defect associated with additional congenital heart disease LIU Jing-jing1,2, WANG Qiang2, LIU Jian-shi1,2△ 2017, 45 (12):  1257-1260.  doi: 10.11958/20170672 Abstract ( 868 )   PDF (318KB) ( 3511 )   Objective To discuss the clinical efficacy of Hybrid therapy in repairing muscular ventricular septal defect (mVSD) associated with additional congenital heart disease in children. Methods The clinical data of 18 patients (9 males and 9 females) admitted to our hospital from December 2012 to December 2016 were retrospectively analyzed. The mainly additional congenital heart diseases were tetralogy of fallot, coarctation of aorta, complete transposition of great arteries, double outlet right ventricle, mitral valve cleft. All of the patients were treated with corrective surgeries of Hybrid therapy under direct supervision. Results Four patients died through the operation. One patient died of postoperative infection, 1 patient died of the complex malformation, which wasn′ t repaired, 1 patient died of mitral valve damage for occluder device replacement, 1 patient died of arrhythmia with unknown reason. One patient received non planned reoperation because of the huge membranous ventricular septal defect for the first stage surgery, in which no mVSD was found. All the rest patients were completed their first stage treatment and discharged from hospital. During the followed ups (1 month to 5 years), no patient died. The occluder devices were in correct position in 14 patients, no residual shunt was found in 12 cases. The cardiac function was normal in 13 cases [ejection fraction (EF)=0.63±0.05]. Conclusion It is safe and effective for the treatment of mVSD using Hybrid therapy, which is especially suitable for patients with complex cardiac malformations and patients with complicated vascular situation. Related Articles | Metrics

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Effects of prophylactic cranial irradiation and its different optional times on prognosis of limited-stage small cell lung cancer HUO Dong-jie1, LU Yan-rong1, WANG Hai-feng1, ZHANG Ling1, Abulikemujiang Adili1, LI Yue2, ZHANG Jin-rong1△ 2017, 45 (12):  1261-1265.  doi: 10.11958/20170823 Abstract ( 962 )   PDF (387KB) ( 3815 )   Objective To observe the impact of the prophylactic cranial irradiation (PCI) and its different interventional times on the prognosis of patients with limited-stage small cell lung cancer (LSCLC) who received comprehensive therapy of complete response (CR). Methods A total of 184 LSCLC patients who received radiotherapy and chemotherapy based on comprehensive treatment were retrospectively analyzed. Patients were divided into two groups based on with or without PCI intervention. There were 50 patients (27.2%) in the PCI group and 134 patients (72.8%) in the non PCI group. The PCI group was subdivided into two groups, PCI1 group (n=20) and PCI2 group (n=30), according to whether patients completed 4 cycles of chemotherapy. Chemotherapy regimen, irradiation method and dose were identical for two groups. Results The brain metastasis rates were 14.0% and 30.6% for PCI group and non PCI group. There was significant difference in brain metastasis rate between the two groups (P＜0.05). The median survival times were 25 months (95%CI: 21.487-28.513) and 17 months (95%CI: 15.175-18.825) for PCI group and non PCI group (P＜0.05). The 1,2 and 3-year survival rates were 54%, 36%,15% and 37%,18%,13% for the two groups. There were no significant differences in brain metastasis rates between PCI1 group and PCI2 group (10.0% and 16.7%). There was no significant difference in median survival time between the two subgroups. Conclusion PCI can reduce the incidence of SCLC brain metastases, and prolong the overall survival time. However, different intervention times of PCI have no significant influence on the prognosis of LSCLC. Related Articles | Metrics

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Antibiotics resistance of Proteus mirabilis isolated from patients with acute diarrhea XIA Lin-lin1, YANG Jie1, HUAN Juan1, GE Hong-yu2, ZHAO Zhan1, LIU Xiao-xia1, WANG Yu-bao1△ 2017, 45 (12):  1266-1270.  doi: 10.11958/20170791 Abstract ( 1059 )   PDF (537KB) ( 3772 )   Objective To investigate antibiotics resistance of Proteus mirabilis isolated from stools of patients with acute diarrhea for the prevention and treatment of its infection and the rational use of antibiotics. Methods Stool samples of acute diarrhea patients were collected in the diarrhea outpatient clinic of the Second Hospital of Tianjin Medical University and Tianjin Medical University General Hospital from 2013 to 2014. Enrichment culture and biochemical identification were used to isolate and identify Proteus mirabilis, which were further performed antimicrobial susceptibility testing and class 1 integron detection. Extended spectrum β-lactamases (ESBLs) phenotype and ESBLs genes (TEM, OXA and CTX-M) were amplified by polymerase chain reaction (PCR), and sequencing were carried on in parts of suspected isolates. ESBLspositive strains were analyzed by pulsed-field gel electrophoresis (PFGE). Results A total of 277 strains of non-repetitive Proteus mirabilis were isolated, and 268 of them were performed antimicrobial susceptibility testing (the remaining 9 strains failed to recover). Relative higher resistant rates were trimethoprim/sulfamethoxazole (30.2%), ampicillin (25.4%), nalidixic acid (25.7%), streptomycin (21.6%) and chloramphenicol (21.3%). The multiple drug resistance rate was 24.6% (66/268). The positive rate of class 1 integron was 22.8% (61/268). Resistance rates to third-generation cephalosporin, ciprofloxacin and imipenem were less than 10%, but 4 isolates were resistant to imipenem, third-generation cephalosporin, fluoroquinolones, trimethoprim / sulfamethoxazole, and chloramphenicol simultaneously. Three cefotaxime-resistant strains (1062, 1505 and 1650) were positive for ESBLs phenotype and harbored CTX-M extended-spectrum β-lactamase genes, among them 2 strains also carried TEM and / or OXA β - lactamase genes. The clustering analysis of pulsed-field gel electrophoresis (PFGE) displayed that the similarities between 1505 and 1650 were 85.7%, and the similarity with 1062 was 58.1%. Conclusion Proteus mirabilis isolated from patients with acute diarrhea in our city show significant multidrug resistance, high positive rate of class 1 integron, and emergence of ESBLs-positive strains resistant to imipenem and fluoroquinolones, which pose a threat to public health. Rational use of antibiotics is important in both clinical and nonclinical settings. Related Articles | Metrics

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The association study of rs5498 (A/G K469E) and rs1799969 (G/A R241G) in intercellular adhesion molecule 1 gene polymorphism with diabetic peripheral neuropathy in Han population REN Zhan-jie1, TENG Xiao-yun2, HUANG Ke-chang2, YU Jian-feng1△ 2017, 45 (12):  1271-1275.  doi: 10.11958/20170629 Abstract ( 775 )   PDF (363KB) ( 4418 )   Objective To investigate the association of genetic polymorphisms of intercellular adhesion molecule 1 (ICAM-1) with diabetic peripheral neuropathy (DPN). Methods A total of 607 type 2 diabetes patients from the Affiliated Hospital of Weifang Medical University were enrolled in this study between June 2013 and December 2014. Rs5498 (A/G K469E) and rs1799969 (G/A R241G) in the ICAM-1 gene were genotyped by using TaqMan allelic discrimination in 295 patients with DPN and 312 subjects without DPN. The distribution of these two SNPs and the genetic influence of ICAM-1 gene polymorphisms on the development of DPN were conducted. Results Genotype distributions of both SNPs were coincided with Hardy-Weinberg equilibrium in the two groups. SNP rs1799969 (G/A R241G) in the ICAM-1 gene showed a high GG genotypic frequency at 96.8% (non DPN) and 99.0% (DPN) respectively. SNP rs5498 (A/G K469E) represented AA and AG genotypes. The values were AA 48.7%/AG 39.4% in non DPN group and AA 51.5%/AG 41.7% in DPN group. There were no significant differences in genotypic distributions and allele frequencies of SNPs rs1799969 (G/A R241G) and rs5498 (A/G K469E) between the patients with DPN group and patients without DPN group (P > 0.05). The dominant（AA+AG） /GG and additive (GG / AA) models of rs5498 (A / G K469E) were associated with higher risk of DPN (ORadjusted=1.585, 1.575 respectively, P < 0.05). To carry A allele was related to the susceptibility of DPN. There was no such association in genetic models of rs1799969 (G/A R241G) and DPN pathogenesis. Conclusion The present study provides evidence that SNP rs5498 E469K (A/G) in the ICAM-1 gene is associated with susceptibility of DPN, and the carrying A allele appears to be a risk of DPN Related Articles | Metrics

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A control study of cognitive function in patients with treatment-resistant depression and first-episode depression JIAN Wei-ying1, GUAN Li-jie1, HUANG Jian-wei2, SU Chan3 2017, 45 (12):  1275-1278.  doi: 10.11958/20170731 Abstract ( 954 )   PDF (323KB) ( 3495 )   Objective To compare the cognitive functions between patients with treatment-resistant depression and first-episode depression. Methods A total of 80 major depressive disorder patients admitted in our hospital from March 2015 to December 2016 were included in this study. The patients were divided into treatment-resistant depression group (n= 40) and first-episode depression group (n=40). Another 40 healthy individuals were used as the control group. The basic data of patients were collected, and Hamilton depression scale (HAMD)- 17 was used to assess the severity of the disease. The performing functions were assessed by trail marking test and stroop color-word test. The attention functions were assessed by digital span test. Memory functions were assessed by Hopkins verbal learning test revise (HVLT-R). After treatment for 6 months, cognitive functions were assessed again in first-episode depression group. Results Compared with control group， the scores of trail marking test (TMT) increased, while the scores of digital span test, stroop color-word test, stroop color colorword test, HVLT-R reduced in treatment-resistant depression group and first-episode depression group (P < 0.05). The scores of TMT, HAMD-17 were lower in first-episode depression group than those of treatment-resistant depression group (P < 0.05). There were no significant differences in other indexes between groups. After six months treatment, the trail marking test score and HAMD-17 reduced, but digital span test, stroop color-word test increased in first-episode depression group (P < 0.05). Conclusion The cognitive function damages severely in patients with treatment-resistant depression and first-episode depression, but there is no obvious difference in severity between two groups of patients. Related Articles | Metrics

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Analysis of 45 cases of maternal death from 2006 to 2016 in a hospital PENG Ying， CHEN Ling△, JIANG Lai 2017, 45 (12):  1279-1282.  doi: 10.11958/20170254 Abstract ( 903 )   PDF (347KB) ( 3464 )   Objective To analyze the causes and influencing factors of maternal mortality and provide the basis for reducing maternal mortality. Methods Forty-five cases of maternal death were collected in our hospital from October 2006 to October 2016, and their age, pregnancy care, cause of death and their assessment were retrospectively analyzed. Results The mortality rate of maternal mortality showed a decreasing trend in 11 years. Seventeen died of direct obstetric factors, and 28 died of indirect obstetric factors. Eighteen cases were inevitable death, and 27 cases were avoidable death. The delivery mothers in our hospital were mainly affected by the individual and family factors of the maternal and family. The pregnant women transferred to our hospital after childbirth were also affected by the knowledge, skills and medical resources of the health care system. Conclusion It is an effective measure to reduce maternal mortality by strengthening propaganda, education and perinatal care, improving maternal health awareness and improving the technical level of medical staff. Related Articles | Metrics

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The relationship between insulin resistance and glucocorticoids in patients with systemic lupus erythematosus JIN Yong-xin, XU Ping, GAO Yan-qing, LIU Zheng, ZHU Gui-jin, ZHAO Mian, ZHOU Lei△ 2017, 45 (12):  1282-1285.  doi: 10.11958/20170866 Abstract ( 1125 )   PDF (322KB) ( 3765 )   Objective To evaluate insulin resistance (IR) in patients with systemic lupus erythematosus (SLE) and investigate the effect of glucocorticoids on IR and its clinical significance. Methods Three hundred and one SLE patients and 103 healthy volunteers hospitalized in our hospital from May 2013 to May 2017 were included in this study. Homeostasis model assessment (HOMA) was used to calculate insulin resistance index (HOMA-IR), HOMA-β cell function index (HBCI) and insulin sensitivity index (ISI). The IR grouping was determined by using the upper 1/4 of the HOMA-IR index of 103 healthy volunteers as the cut point. According to the dose of glucocorticoids in the last 3 months, the SLE group was divided into glucocorticoids＞7.5 mg/d group, ≤7.5 mg/d group and without glucocorticoids group. These parameters were compared with all groups respectively. Furthermore, related factors for HOMA-IR were analyzed by linear correlation. Results Compared with control group, triacylglycerol (TG, P＜0.01), fasting insulin (FINS, P＜0.01), HOMA-IR (P＜0.01), HOMAHBCI (P＜0.05) were significantly increased and high-density lipoprotein cholesterol (HDL-C, P＜0.01) and ISI (P＜0.01) were significantly lower in SLE group. However, there were no significant differences in TG, FINS, HOMA-IR and ISI between different doses of glucocorticoid groups (P＞0.05). The level of HOMA-HBCI was significantly higher in two groups with glucocorticoids than that without glucocorticoid group and normal control group (P＜0.05), while there was no significant difference in HOMA-HBCI between without glucocorticoid group and the normal control group (P＞0.05). HOMA-IR was positively correlated with fasting blood glucose (FBG, r=0.566, P＜0.01), FINS (r=0.949, P＜0.01) and HOMA-HBCI (r=0.280, P＜0.01). But there was a negative correlation between TG (r=-0.139, P＜0.01) and ISI (r=-0.896, P＜0.01). Conclusion Insulin secretion is abnormal and the incidence of IR is elevated in SLE patients. Long term glucocorticoid therapy may be involved in the formation of IR. Related Articles | Metrics

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Clinical features and imaging diagnosis of colonic stenosis in infants HU Li-li, LI Xin-zi, HE Chang-jiang, SUN Yan, WANG Chun-xiang 2017, 45 (12):  1286-1288.  doi: 10.11958/20170592 Abstract ( 1557 )   PDF (437KB) ( 4534 )   Objective To explore clinical features and imaging diagnosis of colonic stenosis in infants. Methods Seven patients with congenital and acquired colonic stenosis proved by surgery were included in this study. The clinical features, erect abdominal plain radiograph and barium enema were analyzed. Results Of the 7 patients, 4 were congenital colonic stenosis with progressive abdominal distention and vomiting. The erect abdominal plain radiograph showed that intestinal inflation in 3 patients, low-set mechanical intestinal obstruction in 1 patient. In the remaining 3 patients who underwent ileostomy after neonatal necrotizing enterocolitis (NEC). Barium enema showed colonic stenosis in 5 patients and 2 were missed diagnosed who underwent contrast examination in the small intestine, and which showed stenosis in ascending colon near the ileocecus. Seven patients were all proved by surgery. The stenosis sites were located in sigmoid colon in 2 cases, in descending colon in 2 cases, in ascending colon in 2 cases and in transverse colon in 1 case. In 4 cases of congenital colonic stenosis, 2 cases underwent surgical staging, 1 case was followed up for half a year, showing normal defecation and well development, the other 1 case was lost visit after hospital discharge. The other 2 cases received end-toend ileum and colon anastomosis, the abdominal distension was relieved in outpatient review, showing well-developed. Three cases with NEC and secondary colonic stenosis underwent staged surgery. Two patients were followed up in outpatient 2 weeks after operation. They were followed up for half a year, showing normal defecation and well-developed. The other 1 case was lost visit after hospital discharge. Conclusion Clinical features of colonic stenosis are very different and depend on the stenosis degree. NEC is the main cause of acquired colonic stenosis and it can be diagnosed by barium enema. Related Articles | Metrics

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The role and mechanism analysis of FAT2 gene in the ovarian epithelial carcinoma based on TCGA datasets ZHAI Hui-hui1, ZHAO Hong-bo2, ZHANG Qin1, YANG Li-hua1△ 2017, 45 (12):  1289-1292.  doi: 10.11958/20170801 Abstract ( 1113 )   PDF (499KB) ( 3979 )   ponding Author E-mail: yang Abstract: Objective To discuss the role of FAT2 gene in the ovarian epithelial carcinoma (OEC) and predict and analyze the possible mechanism and clinical value of FAT2 in the occurrence and development of ovarian cancer. Methods Data of 311 patients with OEC in the TCGA database were analyzed by cbioprotal online analysis platform. Kaplan-Meier method was used to analyze the relationship between the FAT2 genetic alteration and ovarian cancer survival. The FAT2 gene related proteins, protein network and enrichment analysis were performed by GeneCards database. Results In 311 patients, mutation of FAT2 gene was found in 12 patients with a mutation rate of 4%, amplification in 7 cases (2.3%), multiple mutations in 3 cases (1%) and deletion in 2 cases (0.6%). Kaplan-Meier curve showed that the total survival of patients with FAT2 genetic alteration was significantly longer than that in patients without FAT2 genetic alteration (P=0.033 8). There was no significant difference in the disease-free survival time between the two groups (P=0.387). Twenty-one FAT2 related protein, such as CTNNB1, ARVCF and CTNND1 were down expressed in OEC tissues. The FAT2 related protein enrichment was analyzed, and Wnt signaling pathway, cell-cell adhesion and other cell physiological activities were found. Conclusion FAT2 may play the role in cancer suppressor gene in OEC, which induces the degradation of key protein CTNNB1 in Wnt pathway. The Over expression of FAT2 might be a good prognostic factor in ovarian cancer and can be used as a potential prognostic marker and a therapeutic target. Related Articles | Metrics

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Clinical features and related risk factors of sleep hypopnea in obstructive sleep apnea hypoventilation syndrome HOU Wan-ju, WANG Yan, DONG Li-xia△, CAO Jie△ 2017, 45 (12):  1292-1296.  doi: 10.11958/20170868 Abstract ( 874 )   PDF (368KB) ( 3887 )   Objective To analyze the clinical features and related risk factors of sleep hypopnea (SH) in obstructive sleep apnea hypoventilation syndrome (OSAHS). Methods A total of 63 patients with OSAHS who were underwent polysomnography (PSG) and transcutaneous carbon dioxide partial pressure (TCPCO2) monitoring were selected in this study. All patients were divided into pure OSAHS group (n=35) and OSAHS with SH group (n=28) according to the diagnostic criteria of SH. The clinical features of nocturnal carbon dioxide and related risk factors were compared between two groups, including gender, age, complications, body mass index (BMI), Epworth sleepiness scale (ESS), micro awakening index, arterial blood gas analysis, PSG and TCPCO2. Correlation analysis were used to analyze the correlation between the highest TCPCO2 and other variables. The influencing factors of the highest TCPCO2 were analyzed by multiple linear regression analysis. Receiver operating characteristic (ROC) curve analysis was used to analyze the value for related variables in the diagnosis of SH. Results Twenty-eight patients were diagnosed as SH in all the 63 patients with OSAHS, the proportion was 44.4%. There were no significant differences in gender, age and smoking proportion between the two groups. Data of BMI, arterial carbon dioxide partial pressure [p(CO2)], prevalence of hypertension, ESS, apnea hypopnea index, micro arousal index, percentage of nighttime sleep with blood oxygen saturation less than 90%, highest TCPCO2 and TCPCO2 during each sleep stage were significantly higher in the OSAHS with SH group than those in the pure OSAHS group (P＜0.05), while arterial oxygen partial pressure [p(O2)] and the lowest pulse oxygen saturation (SpO2) were significantly lower than those in pure OSAHS group (P＜0.05). The highest TCPCO2 was positively correlated with p(CO2), ESS and BMI (P＜0.01). Multiple linear regression analysis showed that the highest TCPCO2 was affected by BMI and ESS. As a possible predictor for OSAHS with SH, BMI >31.43 kg/m2 showed a sensitivity of 64.3% and specificity of 91.4%, and ESS score >12 showed a sensitivity of 78.6% and specificity of 71.4%. Conclusion The patients of OSAHS with SH have more severe nocturnal hypercapnia and hypoxemia. OSHAS patients are recommend to undergo TcPCO2 monitoring, when BMI is greater than 31.43 kg/m2 and ESS is greater than 12 scores. Related Articles | Metrics

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Efficacy of prophylactic uterine artery embolization on mid-term pregnancy placental site abnormalities and high risk of massive bleeding because of placenta accreta LIANG Yuan, LIU Rong△, LI Hong, LI Hui 2017, 45 (12):  1297-1299.  doi: 10.11958/20170870 Abstract ( 939 )   PDF (710KB) ( 4011 )   Objective To evaluate the safety and efficacy of prophylactic uterine artery embolization (UAE) on mid - term pregnancy placental site abnormalities and massive bleeding because of placenta accreta. Methods Twenty-six patients with prophylactic UAE due to abnormal placental position during pregnancy and placental implantation in our hospital intervention center from Jun 2014 to Jun 2017 were retrospectively analyzed. All patients were treated with prophylactic uterine artery embolization. The clinical data, interventional treatment, complications and follow-up were summarized, and the safety and efficacy of UAE were analyzed. Results Twenty-six patients underwent bilateral uterine artery embolization, the technical success rate was 100%. No major complications related to intervention occurred. All patients were successfully terminated pregnancy after uterine artery embolization. No one had massive bleeding, and all patients were successfully retained the uterus. During the follow-up period, menometrorrhagia was found in a patient for more than six months, and oligomenorrhea was found in 2 patients for five months, and two patients were naturally pregnant again. Conclusion Prophylactic UAE before an obstetrical procedure is a safe and effective way to manage or prevent midterm pregnancy of placental site abnormalities and massive bleeding of placenta accreta Related Articles | Metrics

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Clinicopathologic features and clinical significance of endometrial carcinoma with mismatch repair deficiency PENG Fang-hua1， 2, PANG Shu-jie2, LIU Yi-xin1， 2 2017, 45 (12):  1304-1307.  doi: 10.11958/20170709 Abstract ( 1642 )   PDF (620KB) ( 5316 )   Objective To investigate the clinicopathologic features and clinical significance in mismatch repair (MMR) of deficient endometrial carcinoma. Methods A total of 108 endometrial carcinoma cases younger than 50 years of age who were underwent routine laparotomy in our hospital were included in this study. Immunohistochemistry staining was used for 4 MMR proteins (MLH1, MSH2, MSH6 and PMS2). Patients were divided into two groups (MMR deficiency group and MMR normal group) based on the results of immunohistochemistry staining. Results Thirty-three percent of cases (36 patients) showed at least one deletion of MMR protein expression. The clinicopathological features of 36 cases with deletion of MMR protein expression were analyzed. The 31% showed deep myometrium invasion and 25% were with intense lymphocyte infiltration around tumor cells. Thirty-five percent of endometrial carcinomas associated with mucinous, clear cell or other differentiations, and 14% with heterogeneity. Background endometrium of majority of the cases displayed proliferative endometrium. There were 20% cases complicated with ovarian carcinoma. Features included deep myometrium invasion, lymphocyte infiltration around tumor cells, multiple differentiations and complicated with ovarian carcinoma. There were significant differences in background endometrium between endometrial carcinoma combined with ovarian cancer group and control group. Conclusion MMR deficient endometrial carcinoma has characteristic features, which are different from both type I and type II endometrial carcinoma. Related Articles | Metrics

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Relationship between EGFR mutation and CT features and clinical features in patients with non small cell lung cancer ZHANG Hong1, LYU Jun1, XU Mei-lin2, GAO Guo-zheng2, MA Yan-he1 2017, 45 (12):  1308-1312.  doi: 10.11958/20170154 Abstract ( 961 )   PDF (557KB) ( 3999 )   Objective To investigate the relationship between epidermal growth factor receptor (EGFR) gene mutation and computed tomography (CT) features and clinical features in non-small cell lung cancer (NSCLCs) patients. Methods The clinical data of 187 patients with NSCLCs admitted in our hospital from September 2014 to July 2016 were retrospectively analyzed. All the patients accepted the EGFR mutated gene detection, and they were divided into effective mutation group (n=67) and non-effective mutation group (n=120). The clinical data and lung CT imaging data were complete in the two groups. The univariate and multivariate Logistic regression analysis were used to analyze the differences of imaging and clinical features between the two groups. Results Comparing with the non-mutation group, there were higher proportion of women and lower smoking index in the EGFR effective mutation group. Lesions in the lung tissue showed a clear edge, leaf and burr, and containing ground-glass opacity (GGO) component, usually accompanied by airway bronchogram and pleural indentation, and associated with cancer lymphatic inflammation and lung metastasis in mutation group (all P < 0.05). There were no significant differences in the proportion of necrosis, cavitation, calcification, halo sign and vacuole sign between the two groups (P > 0.05). Logistic regression analysis showed that female, GGO containing composition, burr, air bronchogram, carcinomatous lymphangitis and lesion density showed obvious airway involvement were the predictive risk factors in patients with EGFR effective mutation. Conclusion The EGFR mutation occurs more oftern in female. The reliable predictive signs of CT include GGO composition, burr, airway bronchogram and carcinoid lymphangitis. In critical patients who are not easy to obtain clinical pathology, it has a guiding significance to radiographic assessment for EGFR effective mutation. Related Articles | Metrics

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Clinical value and inference prognosis of plasma perfusion combined with hemofiltration in the treatment of acute paraquat poisoning lung injury and prognosis CHEN Xian, WU Yu-jing, DU Hai-ke△ 2017, 45 (12):  1312-1315.  doi: 10.11958/20170696 Abstract ( 676 )   PDF (348KB) ( 3606 )   Objective To observe the curative effect of plasma perfusion (PP) combined with continuous veno-venous hemofiltration (CVVH) on different degrees of paraquat poisoning induced acute lung injury (ALI), and assess its clinical value for treatment of paraquat poisoning. Methods Data of 197 patients with paraquat poisoning hospitalized in our hospital from June 2013 to April 2017 were retrospectively analysed, including 128 cases with mild-to-moderate poisoning (5 mg·h/L≤SIPP≤25 mg·h/L) and 69 cases with severe poisoning (25 mg·h/L＜SIPP≤50 mg·h/L). Patients were divided into treatment group and control group according to the treatment of PP and CVVH. The incidence rate and time of irreversible lung injury were compared between the two groups of patients. Results (1) In patients with mild-to-moderate poisoning, the incidence rate of lung injury was significantly lower in treatment group than that of control group (P < 0.05). In patients with severe poisoning there was no significant difference in the incidence rate of lung injury between two groups (P > 0.05). (2) There was no significant difference in the occurrence time of irreversible lung injury between two groups of patients with different degrees of poisoning (P > 0.05). (3) The blood toxicant concentration of 4.35 mg·h/L and SIPP value 19.35 mg·h/L were used as a critical indicator of prognosis in treatment group. Conclusion Plasma perfusion combined with continuous veno-venous hemofiltration can obviously reduce the incidence of paraquat poisoning of lung injury in mild-to-moderate patients, but which has no obvious influence on the occurrence time. Related Articles | Metrics

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The relationship between the thrombospondin-1 and the carotid atherosclerosis in patients with type 2 diabetic BIAN Zhi-ying, HU Yan-yun, WANG Jun, SUN Qian, QIE Hai-jing, ZHAO Xin-cui, XIAO Yan-xin, YIN Chao 2017, 45 (12):  1316-1319.  doi: 10.11958/20170771 Abstract ( 653 )   PDF (330KB) ( 4079 )   Objective To investigate the relationship between the thrombospondin-1 (TSP-1) and carotid atherosclerosis and its related indicators in patients with type 2 diabetes mellitus (T2DM). Methods A total of 101 T2DM patients were divided into T2DM group (A group, n = 52) and T2DM with carotid artery atherosclerosis group (B group, n = 49) according to whether complicated with carotid artery atherosclerosis, and 50 normal healthy persons were used as the normal group (C group , n=50). The TSP-1 and other clinical indicators were detected including fasting blood sugar (FPG), fasting insulin (FINS), glycosylated hemoglobin (HbA1c), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), fibrinogen (Fib) and homocysteine (homocysteine). The differences between TSP-1 and other related indicators were analyzed. Results There were no significant differences in diastolic blood pressure (DBP) and body mass index (BMI) between the 3 groups (P>0.05). The level of systolic blood pressure (SBP) was significantly higher in group B than that of group A and group C (P<0.01), but there was no significant difference between group A and group C (P>0.05). There were no significant differences in TC, TG and HDL-C between three groups (P>0.05). The values of TSP-1 and Hcy were increased sequentially in group B, group A and group C (P < 0.05). There were significant differences in FPG, HbA1c, Fib, FINS and LDL-C between three groups (P＜0.05). TSP-1 was positively correlated with FPG, FINS, HbA1c, Fib and Hcy (r= 0.585, 0.341, 0.701, 0.409 and 0.351, P < 0.05). Linear regression analysis showed that TSP-1 was affected by FINS, HbA1c and Fib, and HbA1c was more important. Conclusion TSP-1 is associated with the occurrence and development of diabetic macrovascular complications. It has good clinical value for early detection, early treatment and delaying the progress of diabetic macrovascular diseases. Related Articles | Metrics

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The relationship between traumatic event exposure rate and major depressive disorder prevalence in community population in Tianjin CHEN Zheng1,2, MA Jun1△, XU Guang-ming2△, YIN Hui-fang2 2017, 45 (12):  1320-1323.  doi: 10.11958/20170627 Abstract ( 748 )   PDF (335KB) ( 3509 )   Objective To investigate the prevalence of traumatic event exposure and its relationship with major depressive disorder (MDD) in community population in Tianjin. Methods A multi-stage cluster random sampling method was used for the general health questionnaire (GHQ-12) in the population aged above 18 in 15 538 families of 18 counties in Tianjin city. Among the 11 748 subjects who completed the survey, they were divided into the high-risk group (100%), medium risk group (40%) and low risk group (10%) according to the risk level of mental disorders. Finally, 4 438 residents were interviewed with the diagnostic and statistical manual of mental disorders Fourth Edition (DSM-Ⅳ) axis Ⅰ diagnostic clinical interview version (SCID-Ⅰ/P). The prevalence of respondents exposed to traumatic events and MDD was calculated. Logistic regression analysis was applied to obtain the risk factors of MDD. Results A total of 469 people experienced traumatic events in 4 438 subjects, and the adjusted total exposure rate was 10.3%. Seventy-six people suffered from MDD in 469 cases with traumatic events, and the adjusted total exposure rate was 6.7%. The prevalence of MDD was 3.3% in people with no traumatic events. Logistic regression analysis showed that female (OR=1.77， 95%CI： 1.27-2.46), widowed/ divorced and experienced a traumatic event (OR=2.21， 95%CI： 1.46-3.34) were the risk factors of MDD. Conclusion The total exposure rate of traumatic events is 10.3% in community residents in Tianjin, and the prevalence of MDD is related with the traumatic event exposure. It is suggested that the whole society should mobilize and participate extensively, and pay more attention to the psychological health problems of high risk population. Related Articles | Metrics

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Meta-analysis for efficacy and safety of rosuvastatin and simvastatin in the treatment of hyperlipidemia LIU Shao-yun△, YANG Xue-jia, KONG Li-cha, WANG Dong-ying, DONG Qiu-li, 2017, 45 (12):  1324-1329.  doi: 10.11958/20170615 Abstract ( 755 )   PDF (869KB) ( 3892 )   Objective To investigate the effectiveness and safety of rosuvastatin and simvastatin in the treatment of hyperlipidemia. Methods The database including CNKI, VIP, Wanfang data base and CBM were retrieved to search the clinical randomized controlled trials (RCT) of rosuvastatin and simvastatin in the treatment of hyperlipidemia, and the data were analyzed with Review Manager 5.2. Results Eighteen RCTs were included with a total sample size of 1 819 cases with hyperlipidemia, in which there were 917 patients in rosuvastatin group and 902 in simvastatin group. The Meta-analysis results showed that there were significantly lower serum levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and significantly higher level of high-density lipoprotein cholesterol (HDL-C) in rosuvastatin group compared with those of simvastatin group [(MD=-0.15, 95%CI: -0.22- -0.09, P＜0.01), (MD=-0.18, 95%CI: -0.25- -0.11, P＜0.01), (MD=-0.23, 95%CI: -0.28- -0.19, P＜0.01) and (MD=-0.11, 95%CI: -0.06- -0.15, P＜0.01)]. There was no significant difference in the incidence of gastrointestinal adverse reaction between the two groups. Conclusion The current clinical evidences show that rosuvastatin has a better effect on the treatment of hyperlipidemia, and has no adverse reactions. Related Articles | Metrics

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Error analysis and experience summary in setting up the retroperitoneal cavity for peritoneoscopy CUI Xiao-jian1, CONG Gui-cheng1, YAO Qing-xiang1, LI Bo-quan2 2017, 45 (12):  1330-1333.  doi: 10.11958/20170755 Abstract ( 973 )   PDF (786KB) ( 3646 )   Objective To explore errors and their causes in setting up the retroperitoneal cavity for peritoneoscopy. Methods The clinical data of 450 patients who were performed the laparoscopic surgery in our hospital from May 2009 to December 2016 were collected. According to the trocar puncture points, patients were divided into lumbar group (n=193) and iliac flap group (n=276). The problems were summarized and analyzed in the process of setting up the retroperitoneal cavity. Results The mistakes existed in setting up the retroperitoneal cavity including peritoneum rupture (10 cases), error in balloon expansion clearance (5 cases), homemade balloon rupture and fall off (7 cases), poor position of puncture port (34 cases), bleeding of puncture channel (6 cases), leaking around the trocar and subcutaneous emphysema. After peritoneal patching, re-establishment of the expansion of the gap, adjusting the trocar position and other appropriate measures for treatment, the operations were successfully in 450 patients. Conclusion We should choose the appropriate method for building cavity according to different conditions of patients, and know well the anatomy of the peritoneal cavity. All details should be emphasized in the process of building cavity to reduce the occurrence of errors. Related Articles | Metrics

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Progress of galectin-3 and atrial fibrillation LIU Rui-meng, LIU Chang-le△ 2017, 45 (12):  1330-1333.  doi: 10.11958/20170636 Abstract ( 789 )   PDF (348KB) ( 3113 )   Atrial fibrillation (AF) is the most common tachyarrhythmia in clinical practice. Owing to its high morbidity and mortality, early diagnosis of AF plays an important clinical significance in treatment and prognosis. Galectin-3 (Gal-3), as a sensitive marker of inflammatory and oxidative stress found in recent years, is involved in the occurrence and development of myocardial inflammation and fibrosis, which is closely related to atrial remodeling. Gal-3 inhibitor may decrease the risk of AF by reversing myocardial remodeling, which may provide a new theoretical basis and research direction for the upstream treatment of AF. Related Articles | Metrics

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Research progress of IL-8 in lung cancer MIAO Ya-jing1, JIN Fang2, XU Peng-yu2, QIU Xiao-fei2△ 2017, 45 (12):  1333-1336.  doi: 10.11958/20170724 Abstract ( 727 )   PDF (330KB) ( 3765 )   Lung cancer is the leading cause of cancer-related deaths worldwide. Acquired drug resistance and metastasis are the main causes of treatment failure in lung cancer. Tumor microenvironment is a complex network for the survival and progression of tumor cells, in which inflammatory factors play a critical role in drug resistance and metastasis. Interleukin (IL)-8 is one of critical pro-inflammatory cytokines responsible for cell proliferation, invasion and metastasis, drug resistance and early recurrence in lung cancer. A serum based approach is advantageous for providing a real-time detection and evaluation of disease status in patients. In this review, we summarize the recent advances of IL-8 in predicting prognosis and radiation-induced lung toxicity (RILT), as well as increasing resistance and stem-like characteristics of lung cancer. Related Articles | Metrics

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Study on the role of miRNA-183 family in malignant cancer ZHANG Zi-li1, WANG Jin-liang2, MENG Xiang-chao1△ 2017, 45 (12):  1337-1340.  doi: 10.11958/20170930 Abstract ( 764 )   PDF (313KB) ( 5167 )   MiRNAs have a close relation with the development of malignancy. As a new type of regulatory factor, miRNA plays an important role in the development and progression of tumors, and plays the role of oncogene or tumor suppressor gene. Abnormal expression of miRNA is found in tumors. Most of the abnormal expression of miRNA are related to tumor staging, malignancy, treatment and prognosis, which provides a new strategy for the evaluation of early diagnosis, treatment and prognosis of tumors. The miRNA-183 family is composed of three types of miRNAs including miRNA-96, miRNA-182 and miRNA-183. They present abnormal expressions in tumors of digestive system, urogenital system and respiratory system. More and more researches have been done on the relationship between miRNA-183 family and tumor, and the mechanism has been deeply studied. Related Articles | Metrics

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Progress in isoniazid resistant mutant genes and molecular detection methods of Mycobacterium tuberculosis LI Qian-lin1, FENG Fu-min1, DAI Er-hei2△ 2017, 45 (12):  1341-1344.  doi: 10.11958/20170876 Abstract ( 957 )   PDF (364KB) ( 4351 )   Tuberculosis is a chronic infection caused by Mycobacterium tuberculosis. The high financial burden and high incidence rate of tuberculosis have become a critical public health problem in China. Isoniazid, as one of the important first-line drugs in the treatment of clinical patients, drug resistance is becoming increasingly serious because of the lack of standard treatment. At present, the main mechanism of isoniazid resistance is known as gene mutation. How to detect drug resistance gene effectively and accurately is the key to control tuberculosis. In this article, we analyzed the frequent genes (katG, inhA, ndh, ahpC and oxyR) and novel genes (kasA, sigI, furA, mabA, iniABC and efpA) from the perspective of molecular biology, and summarized their diagnostic methods. Related Articles | Metrics