Table of Content

15 March 2019, Volume 47 Issue 3 Previous Issue    Next Issue

---

For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Select

The low expression of ESE3 under hypoxia and glucose deprived conditions promotes the invasion of PDAC cell line XIAO Di, ZHAO Tian-suo, YU Ming 2019, 47 (3):  225-229.  doi: 10.11958/20190230 Abstract ( 835 )   PDF (890KB) ( 4250 )   Objective To observe the changes of epithelial specific ETS transcription factor 3 (ESE3) expression in PDAC cell line under hypoxia and glucose deprived conditions, and its effect on the invasive ability of PDAC cells.Methods Pancreatic cancer tissues and 16 adjacent normal pancreas tissues from 99 patients with PDAC who underwent radical surgery from January 2005 to October 2014 in Tianjin Medical University Cancer Hospital were collected.Immunohistochemistry was used to analyze the expression of ESE3 in tumor tissues and adjacent normal tissues, and explore the relationship between ESE3 expression level and survival prognosis in PDAC patients. The relationship between ESE3 mRNA expression level and clinic pathological features of 147 patients with PDAC was further validated in TCGA database.The PANC-1 overexpressing ESE3 stably transfected cell line was constructed and validated. Transwell assay was used to observe the invasive ability of PDAC cells with overexpressing ESE3. The expressions of HIF-1α, GRP78 and ESE3 under hypoxic and glucose deprived conditions were detected by Western blot assay. Results The expression of ESE3 was significantly lower in PDAC tissues than that in normal pancreatic tissues. The low expression of ESE3 was closely related to the shorten of overall survival (OS) and non-recurrence survival (DFS, P＜0.05). The TCGA database demonstrated that ESE3 expression was associated with metastasis (P＜0.05). The invasion ability of PDAC cells was increased under hypoxic and glucose deprived conditions, the expression of ESE3 was decreased, and the expressions of HIF-1α and GRP78 were increased (P＜0.05). Conclusion Under hypoxic and glucose deprived conditions, the expression of ESE3 is decreased in PDAC cells, which promotes PDAC cells invasion. Related Articles | Metrics

Select

miR-21 regulates PTEN/AKT pathway to affect the expression of MMP-2 and MMP-9 in podocytes induced by LPS CHEN Xiang, HUANG Ying, LONG Chun-yi, LIAO Pin-hu 2019, 47 (3):  230-234.  doi: 10.11958/20181295 Abstract ( 666 )   PDF (558KB) ( 4049 )   Objective To study the effects and the regulation mechanism of microRNA-21 (miRNA-21, miR-21) on the expressions of matrix metalloproteinase 2 (MMP-2) and MMP-9 in podocytes under lipid polysaccharide (LPS) stimulation in phosphatase and tensin homology deleted on chromosome ten (PTEN) / phosphorylated protein kinase B (pAKT) signaling pathway. Methods The podocytes of 3-8 generation were divided into LPS group and control group. LPS group was given 10 mg/L LPS. The control group was given the same dose of phosphate buffer saline. After 24 h, expressions of miR-21, MMP-2 and MMP-9 were detected by real-time fluorescent quantitative PCR and ELISA, and the correlation between miR-21, MMP-2 and MMP-9 was analyzed. The model of podocyte injury interfered by exogenous miR-21 under LPS stimulation was constructed. The effects of exogenous miR-21 on expressions of MMP-2, MMP-9, PTEN and p-AKT were detected by Western blot assay. Results Compared with the control group, the expressions of miR-21, MMP-2 and MMP-9 increased in podocytes under LPS stimulation, and they were positively correlated (P＜0.05). After the expression of exogenous miR-21 was up-regulated, the expressions of MMP-2, MMP-9 and p-AKT increased but the expression of PTEN decreased (P＜0.05). While the expression of exogenous miR-21 was controlled, the expressions of MMP-2, MMP-9 and pAKT decreased but the expression of PTEN increased (P＜0.05). Conclusion The miR-21 can regulate PTEN / AKT pathway to affect the expressions of MMP-2 and MMP-9 under LPS stimulation in podocytes. Related Articles | Metrics

Select

The protective effect of U0126 on glutamate neurotoxicity in cerebral cortex ZHAO Jing-mi, SUN Li, LIANG Hao, CHENG Yan 2019, 47 (3):  235-240.  doi: 10.11958/20181702 Abstract ( 693 )   PDF (1269KB) ( 3683 )   Objective To investigate the protective effect and possible mechanism of U0126 on glutamate neurotoxicity in brain. Methods Glutamate neurotoxicity model was established by injecting N-methyl-D-aspartate(NMDA) into the cortex of healthy adult male SD rats. First, the optimal modeling conditions were screened using different concentrations (50, 100, 200 mmol/L) of NMDA and different treatment times (3, 6, 12, 24 h). According to the selected optimal conditions, rats were divided into the control group, MAPK / ERK1 / 2 inhibitor U0126 treatment group (2 g / L),NMDA group (200 mmol / L) and different concentrations (0.5, 1, 2 g / L) of U0126 in combination with the NMDA treatment groups. The animals were sacrificed after treatment for 24 h in each group. The brain tissue sections were stained with HE staining to evaluate the tissue damage. Western blot assay was used to detect cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), Caspase-3 (activated form) and phosphorylated ERK1 /2 (p-ERK1/2) expression levels to determine the protective effect of U0126 in glutamate neurotoxic injury in brain tissue. Results (1) NMDA induced excitotoxic damage in rat cortex in a time - and concentration-dependent manner, activated MAPK / ERK1 / 2 signaling pathway,aggravated brain tissue damage, and 200 mmol/L NMDA for 24 h was selected for modeling. (2) Compared with the control group, the expressions of COX-2, iNOS, Caspase-3 (activated form) and p-ERK1/2 increased significantly in the NMDA group. Compared with the NMDA group, the expression levels of COX-2, iNOS, Caspase-3 (activated form) and p-ERK1/2 decreased with the increased concentration of U0126 in U0126+NMDA treatment group, and the area of brain injury decreased significantly. Conclusion U0126 has a protective effect on glutamate neurotoxicity injury in rat cortex, and its mechanism may be related to the inhibition of ERK1/2 activation and downstream signaling pathways of inflammation and apoptosis. Related Articles | Metrics

Select

Study on the therapeutic effect of penetrating resin on bleached early enamel HE Wei-wei, LIN Wei-long, WANG Xiao-ming 2019, 47 (3):  241-244.  doi: 10.11958/20181375 Abstract ( 852 )   PDF (611KB) ( 4605 )   Objective To evaluate the therapeutic effect of penetrating resin on bleached early enamel caries.Methods A total of 135 cattle incisors were immersed in the demineralization fluid for 24 h. After the formation of chalk stains, they were randomly divided into 3 groups (n=45): the bleaching group (bleached with 40% hydrogen peroxide), the infiltration group (treated with infiltrated resin) and the bleaching and permeation group (bleached with 40% hydrogen peroxide and then treated with an infiltrated resin). All samples were pH-cycled for 28 d to simulate oral conditions. The color and surface microhardness (SMH) and surface microhardness recovery (SMHR) of the enamel were determined before and after the lesion. Three sets of color changes [ΔE (post-disease-basic value), ΔE (post-experimental-basic value) and ΔE (post -experimental-post-loss)] and surface microhardness recovery rate were calculated, and osmotic evaluation was performed using scanning electron microscopy. Results There were no significant differences in SMH before and after the lesion formation between the three groups. After the experiment, the SMH and SMHR were in the infiltration group>bleaching infiltration group>bleaching group (P＜0.05). There were significant differences in ΔE (post-experimental-basal value) and ΔE (post-experimental-post-loss) between the infiltration group and the bleaching group (P＜0.05). The whitening effect was higher in bleaching group than that of infiltration group, but still significantly worse than that of bleaching infiltration group.Under the scanning electron microscope, it can be seen that the bleaching and permeating group has relatively uniform resin penetration, and there were different ultrastructures between the cured resin materials. Conclusion The bleaching treatment before resin penetration can effectively improve the microhardness and the whitening effect. Related Articles | Metrics

Select

The influence of endogenous nociceptin/orphanin FQ (N/OFQ) in ischemic arrhythmia by regulating β1-adrenergic receptor in rats XIONG Chang, HAN Yi, GUO Zheng 2019, 47 (3):  245-249.  doi: 10.11958/20182162 Abstract ( 682 )   PDF (747KB) ( 3671 )   Objective To investigate the effect of nociceptin/orphanin FQ (N/OFQ) on ventricular arrhythmias induced by acute myocardial ischemia, and the role of β1-adrenergic receptor (β1-AR) thereof. Methods The rat model of acute myocardial ischemia was prepared by ligating the left anterior descending branch of the coronary artery. A total of 60 rats were randomly divided into 3 groups, namely sham-operation group (Sham group), coronary artery occlusion group (CAO group) and the pretreatment of N/OFQ receptor antagonist (UFP-101) group (U+CAO group). Ten rats were sacrificed at 15 minutes, and another ten rats were sacrificed at 1 hour after CAO. Electrocardiographic data were recorded. The expressions of β1-AR from myocardial cell membrane and whole cell were detected by Western blot assay. The expression of β1-AR mRNA was detected by RT-qPCR. Results Compared with Sham group, ischemic arrhythmias occurred in CAO group, and mainly occurred during 15 minutes after CAO. The pretreatment with UFP-101 can significantly decrease ischemic arrhythmias. At 15 min, compared with Sham group, the expressions of β1-AR and β1-AR mRNA in whole cell were downregulated, and the membrane β1-AR was up-regulated (all P＜0.05) in CAO group. Meanwhile, no significant differences were found in the expression of β1-AR and its mRNA in whole cell (all P＞0.05). The expression of membrane β1-AR was down-regulated (P＜0.05) in U+CAO group. Compared with the CAO group, the expressions of β1-AR and β1-AR mRNA in whole cell were up-regulated while the β1-AR in membrane was down-regulated (all P＜0.05) in U+CAO group. At 1 h,compared with Sham group, the expressions of β1-AR and β1-AR mRNA in the whole cell were up-regulated, and the membrane β1-AR was down-regulated (all P＜0.05) in CAO and U+CAO group. Conclusion Endogenous N/OFQ can regulate the expression of β1-AR in myocardial cell membrane and participate in the process of ischemic arrhythmia in rats. Related Articles | Metrics

Select

Dexmedetomidine alleviates the long-term synaptic plasticity damage induced by repeated exposure of sevoflurane in neonatal rats LIANG Xiao-li, GUI Huan, LIU Cheng-xi, ZHANG Chao, XU Shan, ZHANG Yi, ZHU Zhao-qiong 2019, 47 (3):  250-255.  doi: 10.11958/20182121 Abstract ( 649 )   PDF (657KB) ( 4211 )   Abstract: Objective To investigate the effect of dexmedetomidine (Dex) preconditioning on the long-term synaptic plasticity damage induced by repeated exposure of sevoflurane in neonatal rats. Methods Forty-eight rats were randomly assigned into sevoflurane group (group S), sevoflurane preconditioning group (group DS) and control group (group C), with 16 rats in each group. Rats in group S were intraperitoneally injected with 3 mL/kg normal saline before inhaling sevoflurane for 4 h at d7, d14 and d21. Rats in group DS were injected intraperitoneally with 20 μg/kg Dex dissolved in 3 mL/kg normal saline before 4 h sevoflurane inhalation at the same time points. Rats in group C inhaled carrier gas for 4 h after injecting 3 mL/ kg normal saline. Eight rats were randomly selected from each group at d37 and d97 to detect learning and memory ability by Morris water maze. Thereafter, in vivo electrophysiological experiments were implemented to compare the longterm potentiation (LTP) and paired-pulse facilitation (PPF) ratio in hippocampal neurons between three groups. Results At both juvenile and adult rats, comparing with rats in group C and group DS, the escape latency was remarkably prolonged in rats of group S, and the number of crossing through the target quadrant was reduced (P＜0.05). The increments of field excitatory postsynaptic potential (fEPSP) slope at all time points after high frequency stimulation were remarkably lower in rats of group S than those of group C and group DS (P＜0.05), while PPF ratio was significantly higher at multiple stimulus intervals in group S than group C and group DS (P＜0.05). As compared to group S at juvenile age, the escape latency at adult age was shortened in the first and second day in group S, and the number of crossing was increased. Meanwhile in the group S at adult age, the increment of fEPSP after high frequency stimulation was higher, and the PPF ratios induced by paired stimulation with interstimulus interval of 50 ms,100 ms,150 ms and 200 ms were obviously lower than those of group S at juvenile age (P＜0.05). Conclusion Dexmedetomidine can ameliorate the abnormal changes on the long-term and shortterm synaptic plasticity, as well as learning and memory ability, which could provide a new strategy for prevention and treatment of adverse effects on the developing brain induced by sevoflurane in clinical practice. Related Articles | Metrics

Select

The inhibitive effect of polydatin on the proliferation and invasion in lung cancer A549 cells and its mechanism SUN Bei, YE Yin-tao 2019, 47 (3):  255-259.  doi: 10.11958/20181418 Abstract ( 694 )   PDF (647KB) ( 3799 )   Objective To explore the inhibitory effect of polydatin on the proliferation and invasion in lung cancer A549 cells and its mechanism. Methods The lung cancer A549 cells were treated with different concentrations of polydatin (0, 10, 20, 50, 100 and 200 mg / L) for 24, 48 and 72 hours. The proliferation of A549 cells was measured by tetrazolium nitrogen salt (MTS) assay to determine the concentration of subsequent experiments (10 mg/L and 20 mg/L for 48h). With different concentrations of polydatin (0, 10 and 20 mg/L) for 48 hours, the invasion of A549 cells was measured by Transwell assay. The expression levels of matrix metalloprotinase (MMP)-2/9, integrin β4, Toll-like receptor (TLR3), cluster of differentiation (CD44) and phosphorylation of serine/threonine kinase (p-AKT) were measured by Western blotting and real-time PCR assay. The content of transforming growth factor (TGF) - β and tumor necrosis factor (TNF) - α in culture medium were measured by ELISA assay. The activity of nuclear factor (NF) - κB was measured by report gene assay.Results In the concentration ranges of 0, 10, 20, 50, 100 and 200 mg/L polydatin for 24, 48 and 72 h, the cell proliferation was decreased, and 10 mg/L and 20 mg/L of polydatin treatment for 48 h were used for following experiments. After treatment with polydatin for 48 h, the invasion was decreased, the contents of TGF-β and TNF-α were decreased, and the expression levels of MMP-2 / 9, integrin β4, TLR3, CD44, p-AKT and the activity of NF - κB were also decreased (P＜0.05).Conclusion Polydatin can inhibit the proliferation and invasion of lung cancer A549 cells by regulating the expression of tumor reated gene and the activity of AKT/NF-κB signal pathway. Related Articles | Metrics

Select

Cardioprotective effect of myocardial injection or subarachnoid injection mediated nerve growth factor gene transfection in type 1 diabetic rats YI Jian-min, YUE Wei, GAO Pan, ZHANG Wei-nan 2019, 47 (3):  260-264.  doi: 10.11958/20181625 Abstract ( 528 )   PDF (421KB) ( 4757 )   Objective To investigate the protective effect of recombinant adeno-associated virus (rAAV) mediated nerve growth factor (NGF) transfection by intramyocardial injection or subarachnoid injection in type 1 diabetic rats.Methods The whole experiment consists of two sub-experiments, experiment A:12 SPF grade male SD rats were randomly divided into control group (group control) and diabetes mellitus group (group DM, n=6). Group DM was given streptozotocin(STZ, 50 mg/kg, i.p) to induce type 1 diabetic rat model. The tail flick latencies were measured one day before STZ injection and 1, 2, 4, 6, 8 and 9 weeks after STZ injection in two groups of rats. The contents of NGF and calcitonin generelated peptide (CGRP) were measured by ELISA method. Experiment B:24 SPF grade male SD rats were randomly divided into 4 group (n=6): diabetic heart transfection control group (group MC), diabetic heart transfection group (group ME), diabetic spinal cord transfection control group (group SC) and diabetic spinal cord transfection group (group SE). The 2×2 factorial design was used in this study. The diabetic rat model was established by giving STZ (50 mg/kg, i.p) in four groups of rats. At the end of 4 weeks after injection of STZ, rats in group MC and ME were transfected with rAAV-green florescent protein (GFP) and rAAV-NGF-GFP at a titer of 0.8×1013 virus genomes (μg/L) by intramyocardial injection at 100 μL. Rats in group SC and SE were transfected with rAAV-GFP and rAAV-NGF-GFP at a titer of 0.8 × 1012 virus genomes (μg / L) by subarachnoid injection at 25 μL. After 5 weeks, the cardiac function indexes were measured in groups of rats. The expressions of GFP in myocardium, spinal cord and dorsal root ganglion of T1-T5 were observed by fluorescence microscope,and the contents of NGF and CGRP were measured by ELISA method. Results In experiment A, after 4 weeks, the tail flick latency was significantly delayed in group DM compared with that of group control (P＜0.05). The NGF and CGRP protein in myocardium were significantly decreased in group DM compared with those of group control (P＜0.05). In experiment B, both rAAV-NGF-GFP and myocardial tissue as transfection pathway significantly improved the cardiac function of rats (P＜0.05), and up-regulated the contents of NGF and CGRP protein in myocardial tissue (P＜0.05). There was an interaction between transfectants and transfection pathways during the experiment. Combination of both can improve cardiac function and up-regulate NGF and CGRP in myocardium (P＜0.05). The myocardial point injection of rAAV NGFGFP can significantly improve the cardiac function of rats (P＜0.01), and up-regulate the contents of NGF and CGRP protein in myocardial tissue (P＜0.01). Conclusion Compared with subarachnoid injection, intramyocardial injection of rAAV-NGF can effectively increase the expression of NGF in myocardial tissue of diabetic rats and have a more effective cardioprotective effect. Related Articles | Metrics

Select

The clinical value of eosinophils in the diagnosis and treatment of children with adenoid hypertrophy ZHANG Qi-xue, CAO Yuan, ZHANG Gui-min, ZHAO Xiu-juan, RUAN Hong-ying, ZHENG Yong-zhe, DU Jian-qun, LU Hong-hua, LIN Peng 2019, 47 (3):  265-268.  doi: 10.11958/20181682 Abstract ( 908 )   PDF (515KB) ( 4019 )   Objective To investigate the levels of eosinophils (EOS) in peripheral blood and tissues of children with adenoid hypertrophy, and its correlation with allergic factors. Methods A total of 112 children underwent adenoidectomy in the department of otorhinolaryngology, head and neck surgery of Tianjin First Center Hospital from October 2017 to July 2018 were selected as the study objects. These children were divided into two study groups: adenoid hypertrophy only (AH,n=62) group and adenoid hypertrophy with allergic rhinitis (AH+AR, n=50) group. The number of EOS in tissue sections stained with hematoxylin-eosin (HE) was observed under high power microscope. The percentage of EOS in peripheral blood was detected. In addition, the serum specific IgE detection and visual analogue scales (VAS) were performed in all children,and the differences of the levels of EOS in tissues and peripheral blood were analyzed. The receiver operating characteristic(ROC) curve was drawn to analyze the diagnostic value of the EOS levels in tissues and peripheral blood for allergic factors in children with AH+AR. Results The EOS level was significantly higher in AH + AR group than that in AH group (P＜ 0.05). There were no significant differences in EOS levels between different levels of allergen (P＞0.05). The EOS level in peripheral blood was significantly higher in patients with severe allergic rhinitis than that of patients with mild allergic rhinitis (P＜0.05). There were no significant differences in the EOS levels between severe and moderate rhinitis, moderate and mild rhinitis (P＞0.05). In tissues, the more severe the symptoms of allergic rhinitis are, the higher the level of EOS is(P＜0.05). According to the ROC curve, the area under the curve (AUC): peripheral blood was 0.791 (95%CI: 0.705-0.878，P＜0.001), the cut-off value was 2.60%, and the sensitivity was 70.0%, the specificity was 74.2%; and in tissue AUC was0.843 (95%CI: 0.765-0.920, P<0.001), the cut-off value was 3/10 sections, and the sensitivity was 76.0% and the specificity was 80.6%. Conclusion The EOS levels in adenoid tissue and peripheral blood are increased in children with positive serum specific IgE, suggesting that there are allergic factors are in the etiology of AH. The EOS level in tissues is more important for the differential diagnosis of allergic factors. Related Articles | Metrics

Select

Evaluation of clinical prognostic factors of interstitial lung disease in patients with anti-MDA5 antibody positive dermatomyositis WANG Jie-rui, SHU Rong, SHI Hui-jing, LIU Bai-lu, LI Na, YANG Wen-fang HU Yu-qin, HAN Yi-xuan 2019, 47 (3):  269-273.  doi: 10.11958/20182151 Abstract ( 1675 )   PDF (357KB) ( 5310 )   Objective To analyze retrospectively clinical manifestations and prognostic factors of interstitial lung disease (ILD) in patients with anti-melanoma differentiation-associated gene 5 (MDA5) antibody-positive dermatomyositis(DM). Methods The patients with DM-ILD admitted to Kailuan General Hospital between January 2017 and April 2018 were selected as subjects including 9 patients with anti-MDA5 antibody positive (4 survivors，5 deaths) and 9 patients with anti-synthase (ARS)-antibody positive. The clinical manifestations were compared between the two groups. Cox regression analysis adjusted for age and sex was used to determine prognostic factors for anti-MDA5 antibody-related ILD. Results Compared with ARS-ILD patients, krebs von den lungen 6 (KL-6), alanine aminotransferase (ALT), glutamyltransferase (GGT) and ferritin levels were significantly higher in MDA5-ILD patients (P＜0.05). There were no significant differences in age, sex, incidence of CADM, creatine kinase (CK), KL-6, lactate dehydrogenase (LDH), aspartate aminotransferase (AST),ALT, GGT, C-reactive protein (CRP) and ferritin levels between 4 survivors and 5 dead patients (all P＞0.05). After adjusting confounding factors, the level of ferritin was not associated with the prognosis of anti-MDA5 antibody positive patients. Conclusion Anti-MDA5 antibody is an important index for early diagnosis of DM complicated with acute /subacute interstitial pneumonia (A / SIP). It has not yet been demonstrated that the level of ferritin is associated with the severity of disease and prognosis of anti-MDA5 antibody-related ILD. Related Articles | Metrics

Select

Expression and clinical significance of Panx 1 in non-small cell lung cancer ZHANG Hui-li, MU Yan-xi, TIAN Yu, YANG Li-qun, WANG Lin 2019, 47 (3):  273-276.  doi: 10.11958/20181666 Abstract ( 886 )   PDF (590KB) ( 3763 )   To investigate the expression of Pannexin1 (Panx1) in human non-small cell lung cancer(NSCLC), and to analyze the clinical significance of Panx1 expression. Methods Real-time quantitative PCR was used to detect the expression of Panx1 mRNA in 25 NSCLC and matched paracancerous samples. The expressions of Panx1 protein were detected by immunohistochemical staining in 30 samples of NSCLC and matched paracancerous tissues, and 10 samples of normal lung tissues. The relationship between its expression and clinicopathological features was analyzed.Results The expression levels of Panx1 mRNA and Panx1 protein were significantly higher in NSCLC than those in adjacent tissues (P＜0.05). There were no significant differences in positive expression rates of Panx1 between different age,gender, pleural invasion and pathological grading groups of NSCLC patients (P＞0.05). The positive expression rate of Panx1 was higher in the TNM clinical stage Ⅲ-Ⅳ group than that in the Ⅰ-Ⅱ group. The positive expression rate of Panx1 was significantly higher in the lymph node metastasis group than that in the no lymph node metastasis group (P＜0.05).Conclusion Panx1 is highly expressed in NSCLC tissues, but low in normal lung tissues or adjacent tissues, suggesting that Panx1 may play an important role in the occurrence, development and metastasis of NSCLC. Related Articles | Metrics

Select

Clinical characteristics of secondary interstitial pulmonary disease in patients with rheumatoid arthritis and primary Sjogren's syndrome SONG Guo-jing, QIU Li-jun, PANG Chun-yan, ZHAO Si-meng, LIU Yuan, WANG Yong-fu 2019, 47 (3):  277-280.  doi: 10.11958/20181391 Abstract ( 820 )   PDF (404KB) ( 4586 )   Objective To investigate the clinical features, imaging scores, and prognosis of secondary interstitial lung disease (ILD) in patients with overlapping rheumatoid arthritis and Sjögren’s syndrome (RA-pSS). Methods Clinical data and high-resolution lung CT (HRCT) data of 43 cases of RA-pSS patients were retrospectively collected including 15 cases of RA-pSS associated ILD and 28 cases of RA-pSS with non-ILD. There were no significant differences in age, gender and course of disease between the two groups. The clinical data, lung function, imaging changes in patients with RA-pSS, and score of the lung HRCT were observed, imaging characteristics and prognosis of RA-pSS associated ILD were analyzed. Results Compared with RA-pSS without secondary ILD group, patients with RA-pSS secondary to ILD showed significantly higher levels of C reactive protein (CRP, P＜0.05). RA-pSS secondary to ILD group showed the most common manifestation of interlobular septal thickening in high-resolution CT (HRCT). The most common type of ILD was nonspecific interstitial pneumonia (NSIP) in the RA-pSS secondary ILD group. Compared with the non-UIP group, the clinicalradiology-physiology score (CRP score) was significantly higher in the UIP group (P＜0.05). Compared with the RA-pSS without the secondary ILD group, the survival rate was significantly lower in the RA-pSS secondary ILD group (P＜0.01).Conclusion Patients with RA-pSS associated ILD have higher CRP for their inflammatory markers, complicated imaging characteristic and poor prognosis, and should receive clinical attention. Related Articles | Metrics

Select

Analysis and reflection on the dose-adjustment of antibiotics for patients with chronic kidney disease in continuous renal replacement therapy BAI Jing, LIU Jiang, HOU Juan, HAN Ya-qi, ZHANG Kun, CHEN Xin-ran, HU Zhen-jie 2019, 47 (3):  281-283.  doi: 10.11958/20181797 Abstract ( 635 )   PDF (322KB) ( 3546 )   When continuous renal replacement therapy (CRRT) is performed in critically ill patients, the pharmacokinetics and pharmacodynamic parameters of antibiotics are affected by some factors, such as CRRT, drug properties and pathophysiological condition of patients, which can in turn affect the administration of antibiotics. This article analyzed the dose adjustment of vancomycin in a patient with chronic renal disease treated with CRRT, and discussed the influencing factors of antibacterial dose adjustment in CRRT. Individualized drug administration can ensure the safety and effectiveness of anti - infective therapy. Related Articles | Metrics

Select

The risk factors and perinatal outcome for birth weight ≥2 500 g infants small than gestational age ZHAO Cai-yun, HUA Shao-fang 2019, 47 (3):  284-287.  doi: 10.11958/20181778 Abstract ( 683 )   PDF (343KB) ( 3876 )   Objective To determine the risk factors of birth weight ≥2 500 g infants small than gestational age (SGA) and the common adverse perinatal outcome. Methods A total of 183 infants with birth weight (≥2 500 g) less than gestational age in the Department of Obstetrics of our hospital from January 1, 2013 to April 8, 2018 were selected as the case group. Another 183 cases of appropriate for gestational age (AGA) infants with birth weight ≥2 500 g were selected as the control group. The related indexes affecting neonatal birth weight were compared between two groups, including maternal factors [maternal body mass index (BMI), pregnancy complications], fetal factors (sex, parity), placental and umbilical cord factors. The clinical data of fetal distress, amniotic fluid loss and neonatal diseases were analyzed by SPSS software version 19.0. Results The maternal BMI was less in case group than that of control group, while umbilical cord placenta abnormalities, the ratios of female infants and the first birth were higher in case group than those of the control group (P＜ 0.05). Logistic regression analysis showed that abnormal placenta and umbilical cord, female infants and first fetus were the main risk factors for SGA (≥ 2 500 g), and higher maternal BMI was the protective factor of SGA (≥2 500 g). The incidence of oligohydramnios, fetal distress and birth defects in SGA during perinatal period were significantly higher than those in AGA(≥ 2 500 g, P＜0.05). Conclusion The risk factors and perinatal outcomes for infants (≥2 500 g SGA) have particularity. It is expected that high-risk factors can be closely monitored by strengthening screening and guidance during pregnancy and perinatal period to reduce the occurrence and improve prognosis. Related Articles | Metrics

Select

The clinical data analysis of the dysphagia patients easily misdiagnosed as laryngopharyngeal mass LIU Chun-han, ZHANG Di 2019, 47 (3):  287-289.  doi: 10.11958/20180889 Abstract ( 779 )   PDF (446KB) ( 3525 )   Objective To reduce the occurrence of misdiagnosis of laryngopharyngeal masses by clinicians, reduce the mental and economic burden of patients, and avoid excessive diagnosis and treatment. Methods Clinical data of 20 cases of dysphagia in outpatient department of Otolaryngology and Neurology were retrospectively analyzed. Patients were given neurological and otolaryngological physical examination, magnifying laryngoscope, neck and head CT (computed tomography)scan. Results There were no obvious abnormalities in neurological examination in 20 cases. Under magnifying laryngoscope, the posterior pharyngeal wall mucosa was protruded forward, and the anterior edge of cervical vertebral body was protruded forward on CT scan. After more than one year of clinical follow-up, no significant increase in dysphagia symptoms was observed in 20 patients. Conclusion It is found that cervical hyperplasia can lead to dysphagia, which is easily misdiagnosed as laryngopharyngeal mass. Comprehensive and meticulous neurological system and otolaryngology head and neck surgical examination should be performed, combined with magnifying laryngoscope and neck CT scan to make a definite diagnosis. Related Articles | Metrics

Select

One case of first and second branchial arch syndrome in neonates SONG Li, LIU Yang, WANG Dan 2019, 47 (3):  290-292.  doi: 10.11958/20181796 Abstract ( 1852 )   PDF (596KB) ( 3998 )   The first and second branchial arch syndrome，also known as the hemifacial microsomia (HFM) is a congenital craniofacial deformity. Its etiology and pathogenesis are still unclear. It can rely on the detailed physical examination and imaging examination to diagnose. Surgical and non-surgical treatment can improve facial structure and function, and promote the development of psychosomatic health. Related Articles | Metrics

Select

A case analysis of congenital transverse mesenteric hernia misdiagnosed as small bowel tumor XU Xing, ZHANG Tao, ZHAO Yan, ZHENG Zhi-chao 2019, 47 (3):  293-295.  doi: 10.11958/20181421 Abstract ( 676 )   PDF (375KB) ( 3615 )   Congenital transverse mesenteric hernia is rare in clinical practice. The study reported one case of a patient who was misdiagnosed as small bowel tumor because of symptoms of abdominal distension and CT images showing a mass originated from the initial part of jejunum. However, intraoperative exploration confirmed congenital transverse mesenteric hernia. After the surgical intervention of releasing the hernia sac and repairing the mesenteric hiatus, the patient was discharged from hospital. This study aims to improve the understanding congenital transverse mesenteric hernia and reduce misdiagnosis. Related Articles | Metrics

Select

Clinical investigation of the association between serum homocysteine and cognitive impairment in elderly patients with hemodialysis GUO Yi-dan, TIAN Ru, LUO Yang 2019, 47 (3):  295-298.  doi: 10.11958/20190038 Abstract ( 750 )   PDF (401KB) ( 4301 )   Objective To investigate the relationship between serum homocysteine (Hcy) and cognitive impairment(CI) in elderly patients with hemodialysis (HD). Methods A total of 107 HD patients were divided into normal cognition group and cognitive impairment group according to the evaluation of Montreal Cognitive Assessment (MoCA). Clinical data including serum Hcy level were collected in the two groups. Relevant indicators were compared between the two groups, and the relationship between cognitive impairment and serum level of Hcy was analyzed by multivariate Logistic regression.Receiver operating characteristics (ROC) curve was also plotted to evaluate the diagnostic value of Hcy for the cognitive impairment. Results Seventy(65.4%) of 107 HD patients suffered from cognitive impairment. There were significant differences in MoCA scores, age, gender, education level, stroke history, heart disease history, dialysis duration, hemoglobin and homocysteine levels between the two groups (P＜0.05). Multivariate Logistic regression showed that the increased levels of hemoglobin in male were protective factors for cognitive impairment. The odd ratio (OR) and 95% confidential interval (95%CI) were 0.295 (0.095-0.916) and 0.952 (0.910-0.997，P＜0.05). Age, prolonged dialysis and elevated serum Hcy level were risk factors for cognitive impairment. The OR values (95%CI) were 1.080 (1.026-1.136), 1.022 (1.008-1.036) and 1.212 (1.039-1.414), respectively（P＜0.05). The area under curve of ROC analysis was 0.711 (0.611-0.811), the cut-off point of serum Hcy under the maximum Youden index was 20.65 μmol/L, the sensitivity was 65.7% and the specificity was 56.8%. Conclusion Cognitive impairment is common in hemodialysis patients. It might be associated with gender, age,dialysis duration and anemia. High serum level of Hcy is also an independent risk factor for cognitive impairment, which may be an effective index for predicting cognitive impairment. Related Articles | Metrics

Select

A case report of severe hypoglycemia caused by late dumping syndrome CHEN Fang, HE Qing, HUANG Yu-meng, LIU Ming, CUI Jin 2019, 47 (3):  299-301.  doi: 10.11958/20190031 Abstract ( 806 )   PDF (347KB) ( 3457 )   Dumping syndrome is a common complication after esophageal, gastric and bariatric surgery. This paper introduces a case of late dumping syndrome due to repeated severe hypoglycemia after 8 years of treatment for gastric cardia cancer surgery. After treatment with acarbose, the blood glucose was stable. After follow-up for 2 years, no hypoglycemic reactions such as fatigue and palpitation occurred again. The purpose of this study was to explore the diagnosis and treatment of late dumping syndrome in order to improve the understanding of the disease. Related Articles | Metrics

Select

A case report of de Winter syndrome PI Jian-bin, LI Jun-long, JIN Zheng, WU Wei 2019, 47 (3):  302-304.  doi: 10.11958/20190019 Abstract ( 813 )   PDF (376KB) ( 3580 )   The emergence of de Winter syndrome indicates the occurrence of critical cardiovascular diseases. Serious adverse cardiovascular events can be caused by untimely intervention, but they are easily overlooked because of their rarity and the characteristics of electrocardiogram (ECG). We reported a case of a young man with de Winter syndrome, whose initial complaint was acute chest pain. The result of ECG showed changes of de Winter sign. It was associated with proximal occlusion of the left anterior descending (LAD) coronary artery. Timely reperfusion therapy by means of primary percutaneous coronary intervention (PCI) was the preferred treatment for patient with de Winter syndrome. The subsequent clinical course was uneventful. Therefore, it is necessary to improve the recognition ability of medical workers for such special ECG to improve the prognosis of such patients. Related Articles | Metrics

Select

Targeted surveillance analysis of multidrug-resisitance organisms in a new hospital for five consecutive years WANG Cong-cong, GAO Li, WANG Xiao-cui, REN Peng-fei 2019, 47 (3):  305-308.  doi: 10.11958/20181735 Abstract ( 762 )   PDF (397KB) ( 3715 )   Objective To understand the distribution characteristics and trends of multiple drug resistant organism (MDRO) in a new hospital, and to define the key point of MDRO prevention and control. Methods The target pathogen strains detected from inpatients in Zhengzhou Yihe hospital from July 2013 to June 2018 were collected, and the distribution and change trend of the isolated MDRO were analyzed. Results A total of 7 395 strains of targeted pathogens were detected, including 812 strains of MDRO. The majority of the isolated MDRO plants were carbapenems resistant acinetobacter baumannii (CR-AB, 185 plants, 22.78%), followed by carbapenems resistant Klebsiella pneumoniae (CR-KP,180 plants, 22.17%). The total detection rate of MDRO was 10.98% over the five-year, with an overall upward trend in the average annual detection rate (χ2=20.245, P < 0.001). The main type of MDRO infection was community infection (535 cases,65.89%), mainly concentrated in Neurosurgery, Critical Care, Neurology, and Respiratory Departments. Conclusion The prevention and control of multidrug resistant bacteria are gradually becoming more serious with the extension of the opening time in our hospital. Monitoring and management of CR-AB and CR-KP have become the focus of MDRO prevention and control. Related Articles | Metrics

Select

Meta analysis of clinical efficacy of stem cell therapies for patients with ischemic stroke CHU Yu-ru, YU Nai-hao, KAN Jian-ying 2019, 47 (3):  309-316.  doi: 10.11958/20190037 Abstract ( 731 )   PDF (1045KB) ( 5168 )   Objective To evaluate systematically the clinical efficacy and safety of stem cell therapies for patients with ischemic stroke. Methods PubMed, EMbase, the Cochrane Library, VIP database, Wanfang database and CNKI database were searched by computer about randomized controlled trials of stem cell therapies for patients with ischemic stroke. The retrieval time was from the set up of library to December 2018. Two systematic evaluators screened the literatures according to inclusion and exclusion standards, and extracted the important information of literature. After assessing the quality of included studies, the risk of bias was analyzed by Stata 11.0 software for systematic evaluation and forest plot analysis, and obtained the funnel plot and the sensitivity analysis of included studies. Results A total of 29 case-control studies including total of 1 908 ischemic stroke patients were included in the systematic screening. The results of Meta analysis showed that the neural function deficient degree score (NIHSS), Barthel index (BI), Fugl-Meyer assessment (FMA) and the functional independence measure (FIM) are significantly better in stroke patients with treatment of stem cell transplantation than those of patients in control group. Conclusion Stem cell transplantation can improve effectively the neurological deficit, autonomous behavior ability and body movement function of patients with ischemic stroke. Related Articles | Metrics

Select

Progress in endoplasmic reticulum stress and atrial fibrillation ZHANG Xiao-wei, LI Guang-ping, LIU Tong 2019, 47 (3):  317-321.  doi: 10.11958/20181826 Abstract ( 774 )   PDF (356KB) ( 3895 )   Atrial fibrillation (AF) is a common clinical persistent arrhythmia and an independent risk factor for stroke and heart failure. However, the exact pathogenesis of AF is not completely clear. Endoplasmic reticulum is a major organelle that regulates protein synthesis, intracellular Ca2+ concentration, oxidative stress level, and induces apoptotic signaling pathways. A variety of pathogenic factors can lead to endoplasmic reticulum stress (ERS), which mainly restores the homeostasis of endoplasmic reticulum through the unfolded protein response (UPR). Excessive activation of ERS can lead to Ca2+ overload of atrial myocytes, imbalance of oxidative stress and apoptosis, playing an important role in the pathogenesis of AF. The purpose of this review is to summarize the research progress of ERS and AF. Related Articles | Metrics

Select

Advances in the treatment of knee varus osteoarthritis with high tibial osteotomy ZHANG Hang, HE Sen 2019, 47 (3):  322-328.  doi: 10.11958/20181350 Abstract ( 700 )   PDF (527KB) ( 4788 )   High tibial osteotomy is an option for the treatment of unilateral osteoarthritis of the knee joint in order to relieve pain, improve function, delay knee replacement and preserve high levels of activity in patients. The key to the success of osteotomy is to select patients strictly, comprehensive medical history and physical examination, precise preoperative planning, at the same time, skilled surgical techniques are required as a guarantee. The use of appropriate fixation techniques and rehabilitation treatment schedule can help to achieve long-term good results. This article reviews the indications for high tibial osteotomy, patient selection, preoperative planning, surgical techniques, fixation methods,complications and clinical outcomes. Related Articles | Metrics

Select

Advances in NLRP3 inflammasome and atrial fibrillation WU Xiao-han, LIU Chang-le 2019, 47 (3):  329-332.  doi: 10.11958/20182004 Abstract ( 934 )   PDF (327KB) ( 3856 )   Atrial fibrillation (AF) is the most common tachyarrhythmia in clinic, but its mechanism has not been fully elucidated, and the increased inflammatory response is often associated with the occurrence and development of AF. As the most widely studied and characteristic inflammatory body in recent years, nucleotide-binding oligomerization domain-like receptor protein3 (NLRP3) inflammasome participates in many non-infectious inflammatory reactions, while more evidences show that NLRP3 is closely related to atrial remodeling and myocardial fibrosis, which may provide a new theoretical basis and research target for the upstream mechanisms of AF. In this paper, the characteristics and signal transduction pathways of NLRP3 are briefly described, the correlation between NLRP3 and AF is summarized from both basic and clinical studies,and the future application of NLRP3 antagonist is prospected. Related Articles | Metrics

Select

Progress of microRNAs related to NF-κB signal pathway in rheumatoid arthritis WANG Jing, YUAN Yu-hua 2019, 47 (3):  333-336.  doi: 10.11958/20181531 Abstract ( 709 )   PDF (320KB) ( 5194 )   MicroRNAs are small non-coding RNA molecules that are mainly involved in physiological and pathological processes including cancer, viral infection, inflammation and autoimmune diseases. The nuclear factor κB (NF - κB) is a transcriptional factor with a wide range of gene regulatory functions that participate in different biological processes such as inflammation, cell proliferation, tumor development, learning and memory. Some studies have suggested that microRNA is closely related to the NF-κB pathway and plays an inhibitory or activation role in regulating the NF-κB pathway. This article reviews the microRNAs involved in NF-κB signal regulation in rheumatoid arthritis. Related Articles | Metrics