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15 February 2019, Volume 47 Issue 2 Previous Issue    Next Issue

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Effects of new compound OSU-03012 on proliferation, migration and apoptosis of colon cancer cells LI Yu-qin, SHI Jian-ping, JIN Nu-yun, BU Li-mei, WANG Kai, TIAN Pei-ying 2019, 47 (2):  113-118.  doi: 10.11958/20181265 Abstract ( 744 )   PDF (941KB) ( 3861 )   Abstract: Objective To preliminarily study the anti-colon mechanism of the new compound OSU-03012 at cellular level. Methods The human colon cancer cell line HCT-116, which was intervened with 1 μmol /L, 3 μmol /L, 5 μmol /L and 10 μmol /L of OSU-03012, was used as subjects. HCT-116 cells treated with the same volume of dimethyl sulfoxide (DMSO) were taken as the control group. The inhibition rates of cells in each group after 24, 48 and 72 hours of intervention were detected by CCK-8 method, and the migration ability of cells was detected by scratching test and Transwell assay. The apoptosis of cells was detected by flow cytometry assay, and the expressions of Bcl-2 and Caspase-3 proteins were detected by immunofluorescence assay and Western blot assay. The expressions of Bcl-2 and Caspase-3 mRNA were detected by Real-time PCR. Results The cell inhibition rate and apoptosis rate gradually increased while the cell migration distance and cell migration number gradually decreased in the control group, 1 μmol/L group, 3 μmol/L group, 5 μmol/L group and 10 μmol / L group. There were statistical significance in the multiple comparison between the groups (all P＜0.05). The mRNA and protein expression of Bcl-2 were lower in 3 μmol/L group, 5 μmol/L group and 10 μmol/L group than those in the control group, while mRNA and protein expression of Caspase-3 were higher than those in the control group (both P＜ 0.05). Conclusion The new compound OSU-03012 can inhibit the proliferation and migration of colon cancer cells and promote the apoptosis of colon cancer cells in a dose-dependent manner. Related Articles | Metrics

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Protective mechanism of Ndfip1 on iron overload injury in nerve cells CHEN Ming-hui, TIAN Juan 2019, 47 (2):  118-121.  doi: 10.11958/20181501 Abstract ( 712 )   PDF (492KB) ( 4166 )   Abstract: Objective To investigate the protective mechanism of Ndfip1 overexpression on iron overload injury in nerve cells (SH-SY5Y cells). Methods The SH-SY5Y cells were used as the cell model, and the Ndfip1 plasmid was successfully constructed. SH-SY5Y cells were transfected with Ndfip1 plasmid or empty plasmid, respectively, and the experimental group and the control group were established. The MTT assay was used to detect changes of survival rates of SH-SY5Y cells under different concentrations of FeSO4, and the iron overload concentration was determined. SH-SY5Y cells were transfected with Ndfip1 plasmid to determine the transfection efficiency. Ndfip1 plasmid and empty plasmid were transfected into SH-SY5Y cells, respectively. The survival rates of the two groups were detected by MTT method. The fluorescence intensity was observed by fluorescence microscopy using Calcein AM method to determine the intracellular iron ion contents in two groups of cells. The fluorescence intensity was detected by fluorescence spectrophotometer to determine the changes of iron uptake in two groups of cells. Results As the concentration of FeSO4 increased, the survival rates of SH-SY5Y cells gradually decreased. The 200 μmol/L FeSO4 can cause a significant decrease in survival rate of SH-SY5Y cells. The transfection of SH-SY5Y cells with Ndfip1 plasmid significantly increased the expression of Ndfip1. The overexpression of Ndfip1 can increase the survival rate of iron overload injury, reduce intracellular iron content, and reduce the uptake of iron ions in SH-SY5Y cells. Conclusion Iron overload causes nerve cells to damage, resulting in the decrease in survival rate. Ndfip1 can reduce iron ions into nerve cells, reduce iron accumulation in nerve cells, reduce iron damage to nerve cells, improve survival rate, and play its protective role. Related Articles | Metrics

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Construction of lentiviral vectors to deliver short hairpin RNA of rat Adrb3 gene and its effect on rat vascular smooth muscle cells SONG Yan-qiu, MAO Yong-min, QIN Qin, CONG Hong-liang 2019, 47 (2):  122-126.  doi: 10.11958/20182058 Abstract ( 690 )   PDF (1865KB) ( 5145 )   Objective To construct recombinant lentiviral vectors to deliver short hairpin RNA of rat Adrb3 (rAdrb3) gene and observe the effect of rAdrb3 gene expression on rat vascular smooth muscle cells (VSMC). Methods Two rAdrb3 gene shRNA oligonucleotide sequences were designed, and lentiviral interference plasmids were constructed and sequenced. The lentivirus was packaged by co-transfecting 293T cells with three plasmids, and then the lentivirus titers were measured. Rat VSMC were randomly divided into 4 groups, Normal group, Lv-rAdrb3-shRNA-control group, Lv-rAdrb3-shRNA-1 group and Lv-rAdrb3-shRNA-2 group. The cells were collected at the fifth day after infection. Total RNA and protein were extracted. The expression of rAdrb3 mRNA on rat VSMC was detected by real-time PCR. The expression of rAdrb3 protein on rat VSMC was detected by Western blot assay. Results The recombinant lentivirus was successfully constructed and the titer after purification was 2 × 108 TU/mL. The infection efficiency of the recombinant lentivirus on rat VSMC was 80%. Compared with the Normal group, the silencing efficiencies of rAdrb3 mRNA were 87.18% and 65.27% for Lv-rAdrb3- shRNA-1 group and Lv-rAdrb3-shRNA-2 group (P < 0.05). Compared with the Normal group, the silencing efficiecies of rAdrb3 protein were 85.57% and 70.04% for Lv-rAdrb3-shRNA-1 group and Lv-rAdrb3-shRNA-2 group (P < 0.05). Conclusion The recombinant lentiviral vectors to deliver short hairpin RNA of rAdrb3 gene, which can significantly inhibit the expression of Adrb3 gene on rat VSMC, are constucted successfully. Related Articles | Metrics

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Expression and prognostic significance of KIF23 in breast cancer based on database analysis SONG Shan-shan, ZHANG Qiang, NIE Yan-hong, WANG Yong-fang, CHEN Hao 2019, 47 (2):  127-130.  doi: 10.11958/20181623 Abstract ( 821 )   PDF (839KB) ( 4123 )    Objective To analyze the expression and significance of kinesin family member 23 (KIF23) in breast cancer based on databases mining. Methods In this study, the differential expression of KIF23 gene in breast cancer and normal tissues was compared using the Oncomine and GCBI databases, and then its expression level was overall analyzed. The relationship between KIF23 expression level and prognosis survival, including relapse-free survival (RFS), overall survival (OS), distant metastases-free survival (DMFS) and post-progression survival (PPS), in breast cancer patients were analyzed by the Kaplan-Meier Plotter database. Results It was discovered that KIF23 was more abundantly expressed in breast cancer and a total of 39 studies in the Oncomine database were involved in the expression level of KIF23 in breast cancer and normal tissues. The median rank of KIF23 gene was 900.0 in all differentially expressed genes of breast cancer (P＜0.05). The expression levels of KIF23 were higher in 23 invasive and 4 non-invasive samples of breast cancer, the median rank of differentially expressed genes was statistically significant (P＜0.05). KIF23 was highly expressed in breast cancer (587.27) compared with that of normal tissues (90.89) after integrated analysis (P＜0.05), which was also proved in the GCBI. In addition, Kaplan-Meier Plotter database analysis indicated that the high expression of KIF23 was closely associated with poor survival. Further analysis showed that the expression levels of survival indicators in ER (+) group were statistically significant. Conclusion KIF23 is highly expressed in breast cancer and is associated with poor prognosis in patients, which may be important for the diagnosis, treatment and prognosis assessment of breast cancer. Related Articles | Metrics

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Adenoviral vector mediated C/ebp delta gene improves the function of ischemic heart in mice WANG Dan-dan, TAO Gui-zhou, HUANG Jian-hua, LIU Hua 2019, 47 (2):  131-135.  doi: 10.11958/20181629 Abstract ( 1010 )   PDF (1000KB) ( 4045 )   Objective To investigate the therapeutic effect of adenoviral C/ebp delta vector on ischemic heart disease. Methods The Ad C / ebp delta-EGFP was constructed, and was injected into myocardium to observe the transductive efficiency to the cardiac myocytes. C57BL/6 mice were used in the experiments. The mice were divided into two groups: control group and experimental group. In control group, the Ad-EGFP was injected into myocardium. In experimental group, the Ad C/ebp delta-EGFP was injected into myocardium. The left anterior descending coronary artery was ligated after Ad C/ ebp delta-EGFP was injected into the left anterior wall of the left ventricle. The cardiac function of mice was examined by heart echocardiography. Mice were sacrificed without pain. The hearts were harvested, and the effect of C / ebp delta on myocardial apoptosis was observed with TUNEL staining. The effect of C/ebp delta on angiogenesis was performed by CD31 immunostaining. The effects of C/ ebp delta on the expressions of HIF-1, HO-1 and VEGF proteins in myocardium after myocardial infarction were detected by immunoblotting. Results The Ad C/ebp delta-EGFP was successfully constructed. The expression level of C/ebp delta in the myocardium was significantly higher in experimental group than that of control group. Local injection of adenoviral vector carrying C/ebp delta significantly improved LVFS (0.323±0.031 in experimental group versus 0.221±0.031 in control group, P＜0.05) and LVEF (0.605±0.085 in experimental group versus 0.464±0.071 in control group, P＜0.05). The myocardial cell apoptotic rate was significantly reduced in experimental group (20.36%± 3.07%) than that of control group (44.26%±6.25%, P＜0.01). The vascular density at the border area of myocardial infarction was increased (145.41±15.52 number of capillaries/per view in experimental group versus 77.60±5.19 number of capillaries/ per view in control group, P＜0.01) after heart infarction. The expressions of HIF-1, HO-1, and VEGF proteins were significantly increased in experimental group compared with those of control group (P＜0.01). Conclusion Adenoviral vector mediated C / ebp delta gene may activate HIF-1, which increases expressions of HO-1 and VEGF, resulting in protection of cardiomyocytes and promote angiogenesis in ischemic heart. Related Articles | Metrics

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The role of JAK2/STAT3 signal transduction pathway in the pathogenesis of endometriosis CHEN Jian-mei , YANG Rui-rui, LIU Hong-en, LIU Hong-xin, SHI Wen-xin, LIU Jiao 2019, 47 (2):  136-139.  doi: 10.11958/20181351 Abstract ( 942 )   PDF (892KB) ( 4054 )   Objective To analyze the relationship between the JAK2 / STAT3 signal transduction pathway and endometriosis (EMS). Methods Sixty rats were randomly divided into Sham-operated group, model group and JAK2 / STAT3 pathway inhibitor group (AG group). There were 20 rats in each group. In model group and AG group, EMS model was established by endometrial transplantation. The rats in AG group were given AG490 1 mg/kg 5 min before modeling, and AG490 4 mg/kg after surgery, 2 times a week for 4 weeks. The model group was given same volume of normal saline. After treatment, the volume of ectopic endometrium was recorded, the growth inhibition rate of ectopic endometrium was calculated, and the histopathological changes were observed by HE staining. The protein expression levels of p-JAK2, JAK2, p-STAT3 and STAT3 were detected by Western blot assay. Results After treatment, the volume of ectopic endometrium was significantly smaller in AG group than that in model group, and the inhibition rate of ectopic endometrial growth was 65.66%. The results of Western blot assay showed that JAK2 and STAT3 proteins and their phosphorylation levels (p-JAK2/ JAK2 and p-STAT3 / STAT3) were significantly increased in ectopic lesions of EMS rats compared with those in normal uterus. The expression levels of p-JAK2, p-STAT3, JAK2 and STAT3 were significantly decreased in ectopic lesions of AG group rats compared with those in EMS rats. Conclusion The JAK2/STAT3 pathway may play an important role in the pathogenesis of EMS, and the inhibition of JAK2/STAT3 signaling pathway can play a positive role in the treatment of EMS. Related Articles | Metrics

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The role of NMDA receptor subunit 2A phosphorylation in hepatic ischemia reperfusion induced brain injury and long-term cognitive dysfunction in young mice WANG Xi-hui , YU Xiang-yang, YU Wen-li , DU Hong-yin 2019, 47 (2):  140-144.  doi: 10.11958/20180973 Abstract ( 784 )   PDF (693KB) ( 3747 )    Objective To evaluate the effect of hepatic ischemia reperfusion on hippocampus and long-term cognitive function in young mice, and investigate the mechanisms related to N-methyl-D-aspartic acid receptor subunit 2A (NR2A) phosphorylation. Methods Fifty-four healthy C57 mice, 2-week old, with body mass 6-9 g, were randomly divided into three groups (n=18 / group): sham-operated group (S), hepatic ischemia reperfusion group (I) and NR2A inhibitor NVP- AAM077 treatment group (N). The mice in group N were given the intraperitoneal injection of NVP-AAM077 at the dose of 10 mg/kg for 3 days after the surgery. The mice in group S and group I were given equal amount of normal saline in the same way. Ten mice from each group were sacrificed 3 d after the surgery. Serum concentrations of S100β and neuron-specific enolase of enzyme (NSE) were detected by ELISA. Histopathological changes of hippocampus were determined by HE staining. Western blot assay was used to detect the expression levels of cleaved caspase-3 and NR2A, p-NR2A Y1325. Long-term cognitive function was evaluated by Morris water maze 30 d after surgery. Results Compared with group S, the serum concentrations of S100β and NSE increased significantly, hippocampal tissue was edematous, the cell arrangement was disorder and sparse, the expression levels of cleaved caspase-3 and p-NR2A Y1325 were up-regulated, and the time spent in the target quadrant decreased in group I and group N (P＜0.05). Compared with group I, the serum concentrations of S100β and NSE decreased significantly, morphological changes of hippocampal tissue were ameliorated, the expression levels of cleaved caspase-3 and p-NR2A Y1325 were down-regulated, and the time spent in the target quadrant increased in group N (P＜0.05). There were no significant differences in the expression level of NR2A and escape latency between the three groups (P＞0.05). Conclusion Hepatic ischemia reperfusion could induce brain injury and long-term cognitive dysfunction in young mice, and the mechanism may be associated with the excitotoxicity mediated by excessive phosphorylation of NR2A Y1325. Related Articles | Metrics

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Validation and comparison of pretest probability of coronary artery disease by using coronary CTA in Chinese patients with stale chest pain WANG Yue, LIU Yu-jie, ZHANG Ying, ZHOU Jia 2019, 47 (2):  145-150.  doi: 10.11958/20181261 Abstract ( 995 )   PDF (554KB) ( 5020 )    Objective To compare the performance of updated Diamond–Forrester method (UDFM) and Duke clinical score (DCS) in a Chinese population referred to coronary computed tomography angiography (CCTA). Methods A total of 5 743 consecutive patients with suspected coronary artery disease (CAD) who underwent CCTA for stable chest pain were collected in Tianjin Chest Hospital from December 2015 to December 2017. CAD was defined as stenosis ≥50% in diameter at least one segment of the coronary artery by CCTA. For each patient, pretest probability (PTP) of CAD was estimated according to UDFM and DCS, respectively. Area under the receiver operating characteristic curve (AUC), net reclassification improvement (NRI) and Hosmer–Lemeshow goodness-of-fit statistic (H-Lχ2 ) were assessed to validate and compare the two models. Results Overall, 1 872 (32%) patients were diagnosed as CAD by coronary CTA examination. There were no significant differences between DCS (AUC=0.772, 95%CI: 0.759-0.786) and UDFM (AUC=0.765,95%CI:0.751-0.779, P= 0.068). DCS demonstrated the improved classification over UDFM through the positive NRI (26.20%, P＜0.001). The calibration of UDFM (H-L χ2 =137.823) and DCS (H-L χ2 =156.704) was both unsatisfactory (P＜0.01), and both overestimated the actual prevalence of CAD. Conclusion Compared to UDFM, DCS is superior for estimating the PTP of CAD in Chinese population referred for CCTA, but both overestimate the actual prevalence of CAD. Related Articles | Metrics

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Study on the treatment strategy of NSTE-ACS non-culprit lesions guided by intravascular ultrasound MA Ke-jing, LIU Yu-jie, ZHANG Ying 2019, 47 (2):  150-154.  doi: 10.11958/20181577 Abstract ( 736 )   PDF (407KB) ( 4103 )   Objective To observe the efficacy of intravascular ultrasound (IVUS) and coronary angiography (CAG) in the treatment of non-culprit lesions (NCL) in patients with non-ST-segment elevation acute coronary syndrome (NSTE- ACS) with multi-vessel disease. Methods A total of 295 patients were included in NSTE-ACS with multivessel disease and successfully underwent percutaneous coronary intervention (PCI) for criminal lesions (CL). Patients were randomly divided into IVUS-guided treatment group (n=148) and CAG-guided treatment group (n=147). The IVUS group underwent gray-scale IVUS examination of NCL. Lesions with plaque burden (PB) ≥70% were detected by PCI. Patients in CAG group with the diameter of NCL greater than 90% were performed for PCI, and all patients were given strict secondary prevention drug treatment. The major adverse cardiovascular events (MACE) and disease-related quality of life assessment were followed up and compared between the two groups. Results (1) The stent implantation rate was significantly higher in the IVUS group than that of CAG group (P＜0.05), but the number of stent implantation was lower in IVUS group (P＜0.05). (2) There was no significant difference in the average hospital cost between the two groups (P＞0.05). (3) The follow-up showed that there was no significant difference in MACE incidence between the two groups (5.52% vs. 1.37%， χ2 =2.589， P＞0.05). The degree of physical activity limitation, angina pectoris frequency, and disease cognition score were significantly better in the IVUS group than those of the CAG group (P＜0.05). The Kaplan-Meier curve was used to compare the event-free survival time of the two groups, and there was no significant difference between the two groups (94.5% vs. 98.6％， Log-rank χ2 =3.276，P＞0.05). Conclusion IVUS-guided NCL therapy can reduce the average number of stent implantation, optimize stent implantation, not increase costs in treatment, and improve the quality of life. Related Articles | Metrics

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The expression and significance of serum caveolin-1 in patients with pulmonary hypertension associated with chronic obstructive pulmonary diseases HOU Wan-ju, CAO Jie, WANG Juan 2019, 47 (2):  155-158.  doi: 10.11958/20181303 Abstract ( 776 )   PDF (367KB) ( 4647 )   Objective To study the expression of serum caveolin-1 (Cav-1) in patients with pulmonary hypertension (PAH) associated with chronic obstructive pulmonary disease (COPD), and to analyze its clinical significance. Methods According to whether combined with PAH, a total of 65 stable COPD patients were divided into COPD group [pulmonary systolic blood pressure (PASP) ＜40 mmHg, 35 cases] and COPD-PAH group (PASP ≥40 mmHg, 30 cases). Thirty healthy volunteers who received physical examination in our hospital during the same period were selected as normal control group. Baseline data, blood gas analysis, lung function indicators, and expression levels of Cav-1, IL-6, and TNF - α were compared. The receiver operating characteristic curve (ROC curve) was plotted and the optimal cut-off value for Cav-1 diagnosis of COPD combined with PAH was analyzed. Results The first second forced respiratory volume (FEV1)/forced vital capacity (FVC), FEV1 percentage of predicted value (FEV1%), and oxygen partial pressure [p(O2)] were lower in the COPD-PAH group and the COPD group than those in the control group, and the partial pressure of carbon dioxide [p(CO2)] and PASP were higher than those in the control group (P＜0.01). The p(O2) was lower in the COPD-PAH group than that in the COPD group, and the p(CO2) and PASP were higher than those in the COPD group (P＜0.01). The expression levels of Cav-1 were decreased gradually in the control group, COPD group and COPD-PAH group, while the expression levels of IL- 6 and TNF-α were increased gradually (P＜0.01). For predicting the risk of COPD combined with PAH, the area under the ROC curve was 0.902, the optimal cut-off value was 6.66 ng/ml with a sensitivity of 76.7% and a specificity of 85.7%. Cav-1 was consistent with the PASP results measured by Doppler ultrasound (Kappa value = 0.627). Conclusion The serum expression of Cav-1 is down-regulated in the patients with PAH associated with COPD, and Cav-1 can be a novel serum marker for the diagnosis of PAH associated with COPD. Related Articles | Metrics

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Analysis of influencing factors of radiation-induced lung injury in patients with esophageal cancer treated by IMRT WU Wan-yan, TAN Yao, XIAO Nan, LIU Yan, ISLET Dahl 2019, 47 (2):  159-162.  doi: 10.11958/20181557 Abstract ( 672 )   PDF (350KB) ( 3528 )   Objective To explore the influencing factors of radiation-induced lung injury (RILI) in esophageal cancer treated with intensity modulated radiation therapy (IMRT). Methods Clinical data of 233 patients with esophageal cancer treated with IMRT in Tumor Hospital affiliated to Xinjiang Medical University from January 2014 to December 2016 were analyzed retrospectively. All of the patients were divided into 2 groups according to whether acute radiation lung injury occurred: with RILI and without RILI. The influencing factors of radiation-induced lung injury were analyzed. Clinical data included age, sex, T staging, lymph node metastasis, pathological type, tumor location, tumor length, chemotherapy mode, KPS score, basic lung disease, diabetes and smoking history. Physical parameters of radiotherapy included double lung V5, V10, V15, V20 and V30, mean lung dose (MLD), gross tumor volume (GTV), two lung volume, GTV volume and two lung volume ratio, field number of irradiated and prescription dose. The risk factors of radiation-induced lung injury were screened by Logistic regression analysis. Results In all groups, there were 63 (27%) patients with RILI, and 25 (10.7%) patients with grade ≥2 RILI. Univariate analysis showed that the positive lymph nodes, GTV volume, GTV volume and two lung volume ratio, MLD, and double lung V5, V10, V15, V20, V30 and prescription dose were significantly different between the two groups. Multivariate analysis showed that V5 ≥63.15% (OR=1.022, 95%CI: 1.003-1.041) and V30 ≥5.35% (OR= 2.693, 95%CI: 1.159-6.010) were risk factors for RILI (P＜0.05). Conclusion The full account of the clinical characteristics and the physical indexes such as V5 and V30 should be considered to minimize the risk of RILI, when evaluating the treatment plan of intensity modulated radiotherapy for esophageal cancer. Related Articles | Metrics

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Effects of ultrasound-guided SAPB (serratus anterior plane block) on postoperative analgesia in patients undergoing thoracoscopic lobectomy ZONG Yu, XIE Yan-hu, HU Li-guo, ZHOU Wei-de, CHAI Xiao-qing 2019, 47 (2):  163-166.  doi: 10.11958/20181314 Abstract ( 816 )   PDF (392KB) ( 3958 )    Objective To investigate the effect of ultrasound-guided serratus anterior plane block (SAPB) on postoperative analgesia in patients undergoing thoracoscopic lobectomy. Methods Sixty patients scheduled for thoracoscopic lobectomy under general anesthesia were randomly divided into two groups, group P (intravenous patient- controlled analgesia) and group S (intravenous patient-controlled analgesia+SAPB). Patients received morphine intravenous patient-controlled analgesia (PCIA) after operation in the two groups. Patients of group S were given ultrasound-guided SAPB with 0.4 mL/kg of either 0.375% ropivacaine after the operation immediately. Morphine cumulative consumption at the 6th, 12th and 24th postoperative hours and NRS (numerical rating scale) pain scores at the 30 min, 1, 2, 6, 12 and 24 postoperative hours were recorded respectively in both two groups. Flurbiprofen axetil 50 mg was injected intravenously when the NRS pain score was greater than 4, and it could be repeated if necessary. Adverse reactions such as analgesic rescue cases, postoperative RASS sedation score and nausea, vomiting, pruritus, respiratory depression, bradycardia and hypotension were recorded. Results The NRS pain scores at the 30 min and 1, 2, 6 h were significantly lower in group S than those in group P (P＜0.01). There were no significant differences in NRS pain scores at 12 and 24 h after operation between two groups. NRS pain scores were gradually reduced with the prolongation of postoperative time in two groups. There was no interaction between groups and processing times (P＞0.05). Morphine cumulative consumption were significantly lower at 6, 12 and 24 postoperative hours in group S than those in group P (P＜0.01). No significant differences were found in the incidence of adverse reactions, RASS sedation scores and analgesic rescue cases between two groups. Conclusion Ultrasound-guided SAPB can significantly reduce postoperative morphine consumption and early pain scores in patients undergoing thoracoscopic lobectomy, without increasing the incidence of adverse reactions. Related Articles | Metrics

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Significance of P53 expression in invasive breast cancer YUAN Ming-ming, REN Xiao-yan, TAO Yu-mei, CHEN Ting-ting, CAI Nan-nan, JIN Xiao-xia, WEI Ying-ze, HE Song 2019, 47 (2):  167-170.  doi: 10.11958/20181378 Abstract ( 1854 )   PDF (1292KB) ( 4173 )   Objective To investigate the expression of P53 and its correlation with clinicopathological characteristics in invasive breast cancer. Methods A total of 709 cases of invasive breast cancer were collected in the Nantong Cancer Hospital from January 2015 to December 2017. The expressions of P53 in 709 cases were detected with immunohistochemistry. The expression of P53 and its correlation with molecular classifications, histologic grades and expressions of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER-2) and Ki-67 were investigated and analyzed. Results The high positive rate of P53 was 26.8% (190/709), low positive rate of P53 was 73.2% (519/709). The high positive rate of P53 in luminal subtype A, luminal subtype B with negative HER-2, luminal subtype B with positive HER-2, HER-2 over-expressing subtype and triple negative subtype were 6.1% (12/197), 18.0% (34/189), 39.1% (25/64), 50.0% (58/116) and 42.7% (61/143), respectively. There were significant differences in expressions of P53 between five luminal subtypes (P＜0.05). The expression level of P53 was higher in breast cancer with medullary histological features than that in other histologic subtypes (P＜0.05). The expression of P53 increased with the rise of histologic grades (P＜0.05). The expression level of P53 was higher in breast cancer with ER negative, PR negative, HER-2 positive and high Ki-67 expression groups (all P＜0.05). Conclusion P53 plays a significant role in the development and progression of invasive breast cancer. The expression of P53 can be used to guide the individualized therapy and as a reference index to clinical outcome prediction in invasive breast cancer. Related Articles | Metrics

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Application of dexmedetomidine combined with propofol on mammotome operation for breast ZHANG Li, HE Xian-bing 2019, 47 (2):  171-174.  doi: 10.11958/20181085 Abstract ( 767 )   PDF (369KB) ( 4408 )   Objective To investigate the anesthetic effect of dexmedetomidine combined with propofol in mammotome operation for breast. Methods Sixty patients underwent mammotome operation for multiple solid masses in breast were randomly divided into observation group and control group (n=30 for each group). The control group was anesthetized with propofol. The observation group was anesthetized with dexmedetomidine and propofol. Data of blood pressure, heart rate, oxygen saturation, the time of jaw support, the time of postoperative recovery, the amount of propofol at the beginning of anesthesia, the start of surgery, 5 minutes after the start of surgery, the end time of surgery and the VAS score, Ramsay sedation score at 1 hour after surgery were compared between the two groups. Results There were no significant differences in blood pressure and oxygen saturation between the two groups at each time point (P＞0.05). The heart rates at the beginning of surgery, 5 minutes after the start of surgery and at the end of surgery were lower in the observation group than those of the control group (P＜0.05). The heart rates at the beginning of surgery and 5 minutes after the start of surgery were lower than at the start of anesthesia and the end of surgery in the observation group (P＜0.05). The time of jaw support, recovery time and propofol dosage were shorter in the observation group than those in the control group (P＜0.05). The VAS score was lower at 1h after operation in the observation group than that of the control group. There was no significant difference in the Ramsay score between the two groups (P＞0.05). Conclusion The use of dexmedetomidine combined with propofol on mammotome operation for breast can reduce the amount of propofol. The effect of anesthesia and postoperative sedation is good, but heart rate decreases, which is not suitable for patients with sinus bradycardia and atrioventricular block. Related Articles | Metrics

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The diagnostic value of combined detection of serum RF, anti-CCP and 14-3-3η protein for rheumatoid arthritis YANG Ya-qiong, YANG Hong-wei, BAI Xue, WANG Yi, GUAN Shi-zhen 2019, 47 (2):  174-178.  doi: 10.11958/20181515 Abstract ( 1012 )   PDF (374KB) ( 4217 )   Objective To evaluate the clinical value of combined detection of serum rheumatoid factor (RF), anti- cyclic citrullinated peptide antibody (anti-CCP）and 14-3-3η protein in the diagnosis of rheumatoid arthritis (RA)． Methods A total of 94 RA patients were selected as RA group, and 40 healthy people with age, sex matched were used as control group. Serum 14-3-3η protein and anti-CCP were analyzed by enzyme linked immunosorbent assay (ELISA). RF levels were measured by scattering immune turbidimetry. Series and parallel tests were carried out respectively with the above 3 indexes. The sensitivity, specificity, positive predictive value, negative predictive value, accuracy and Youden’ s index (YI) were used to analyze the diagnostic efficacy of each test for RA. Results Among anti-CCP, RF and 14-3-3η protein, anti-CCP showed the highest accuracy (87.4%), YI (0.753) and the highest sensitivity (84.0%), therefore， it was the best measure of RA diagnosis. When using two serum makers, anti-CCP together with RF were an ideal combination with the highest accuracy (87.4%), YI (0.742) and the highest sensitivity (90.4%). While using three serum markers, [(14-3-3η + anti-CCP) / RF], [(14-3-3η + RF) / anti-CCP], [(14-3-3η / RF) + anti-CCP] and [(14-3-3η+anti-CCP) / (14-3-3η+ RF) / (anti-CCP + RF)] showed the higher accuracy (87.4%, 87.9%, 87.4%, 87.9%) and YI (0.745, 0.756, 0.757, 0.760). [(14-3- 3η + anti-CCP) / RF], [(14-3-3η + RF) / anti-CCP] and [(14-3-3η+anti-CCP) / (14-3-3η+ RF) / (anti-CCP + RF)] were the higher sensitivity (88.3%, 89.4%, 87.2%) than [(14-3-3η / RF) + anti-CCP] (81.9%), which was an ideal combination. Conclusion Among anti-CCP, RF and 14-3-3η protein, anti-CCP has the highest accuracy, YI has the highest sensitivity. The combination detection [(14-3-3η + anti-CCP) / RF], [(14-3-3η + RF) / anti-CCP] and [(14-3-3η+anti- CCP) / (14-3-3η+ RF) / (anti-CCP + RF)] has higher accuracy and higher YI, along with good sensitivity. Related Articles | Metrics

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Comparison analysis of risk factors, stroke mechanisms and infarct patterns between intracranial anterior and posterior circulation in ischemic stroke patients LIN Huan, WANG Jian, LYU Zhi-yu, CHEN Xiu 2019, 47 (2):  179-183.  doi: 10.11958/20181615 Abstract ( 813 )   PDF (392KB) ( 5078 )    Objective To investigate risk factors, stroke mechanisms and infarct patterns of anterior and posterior circulation in ischemic stroke (IS) patients caused by intracranial atherosclerotic stenosis (ICAS). Methods Data of 257 ICSA related IS patients admitted to the department of neurology in our hospital were retrospectively collected from December 2014 to December 2017. According to the location of responsible vessel, all patients were divided into anterior circulation group (n=170) and posterior circulation group (n=87). The clinical data of those patients were collected. The risk factors, stroke mechanisms and infarct patterns were analyzed and compared between the two groups. Results (1) The proportion of male and smoking history was separately higher in anterior circulation group than that in posterior circulation group, while the proportion of hypertension and diabetes, and the level of HbA1c were lower in anterior circulation group than those of posterior circulation group (P＜0.05). Binary logistic regression analysis revealed that hypertension, diabetes and high level of HbA1c were independent risk factors for IS patients caused by intracranial posterior circulation atherosclerotic stenosis. (2) The proportion of hemodynamic impairment and multiple mechanisms were higher in anterior circulation group than those of posterior circulation group, but the proportion of local branch occlusion was lower in anterior circulation group than that of posterior circulation group (P＜0.05). (3) The proportion of single corticosubcortical infarction was higher in anterior circulation group than that of posterior circulation group, however, the proportion of single subcortical infarction was lower than posterior circulation group (P＜0.05). Conclusion There are differences in risk factors, stroke mechanisms and lesion patterns between anterior and posterior circulation in IS patients caused by ICAS. The development of different control strategies for different parts of blood vessels may be beneficial to reduce the occurrence of related IS. Related Articles | Metrics

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Effects of repetitive transcranial magnetic stimulation on motor function and serum levels of MMP-9/hs-CRP of ischemic stroke patients LIU Chao-meng, MEGN Yao, LI Hao-hao, ZHANG Gui-qing 2019, 47 (2):  184-188.  doi: 10.11958/20181534 Abstract ( 669 )   PDF (380KB) ( 5021 )    Objective To evaluate the effects of repetitive transcranial magnetic stimulation (rTMS) on motor function and serum levels of matrix metalloproteinase-9 (MMP-9)/high-sensitivity C-reactive protein (hs-CRP) in patients with ischemic stroke (IS). Methods A total of 54 patients met the inclusion criteria were randomly divided into low-frequency stimulation group, high-frequency stimulation group and sham stimulation group with 18 in each group. All the patients were given routine medical treatment and rehabilitation training. Then the 0.5 Hz rTMS treatment was performed on the healthy side in the primary motor cortex (M1) of the low-frequency stimulation group, and the 10 Hz rTMS treatment was performed on the affected side of the high-frequency stimulation group. It was worth noting that the sham stimulation was also performed on the affected side of the M1 area, but no current, only sound, and the treatment lasted 3 weeks, 5 d per week. Finally, serum levels of MMP-9 and hs-CRP were measured by enzyme-linked immunosorbent assay (ELISA) at 3 days before treatment and 3 weeks after treatment, and the simple motor function on the affected side was evaluated by the FMA scale, meanwhile, the motor evoked potential (MEP) latency and central motor conduction time (CMCT) were recorded. The motor function and serum expression levels of MMP-9 and hs-CRP were compared before and after treatment between the three groups. Results There were no significant differences in serum concentrations of MMP-9 and hs-CRP, FMA scores, MEP latency and CMCT before treatment between the three groups (P＞0.05). After 3 weeks of treatment, the serum concentration of MMP-9 and hs-CRP were lower in low frequency stimulation and high frequency stimulation groups than those of the sham-stimulated group, and the FMA scores of the upper and lower limbs were higher than those of the sham- stimulated group, and the MEP latency/CMCT were shorter than those of sham-stimulated group. There were no significant differences in above indicators between low frequency stimulation and high frequency stimulation groups (P＜0.05). After 3 weeks of treatment, the serum concentrations of MMP-9 and hs-CRP were negatively correlated with the corresponding upper and lower FMA scores in the rTMS treatment group (low and high frequency groups, P＜0.01). Conclusion rTMS can accelerate the recovery of motor function in patients with IS, which may be related to the down-regulation of serum expression levels of MMP-9 and hs-CRP. Related Articles | Metrics

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A case report of IgG4-related disease with lung adenocarcinoma TANG Lu, KONG Chun-yu, QI Wu-fang 2019, 47 (2):  189-191.  doi: 10.11958/20181634 Abstract ( 905 )   PDF (441KB) ( 4249 )    IgG4-related disease (IgG4-RD) is an immune-mediated inflammation with fibrotic disease, and it is clinically found that some IgG4-RD can be combined with malignant tumors. Based on the retrospective analysis of 1 case of IgG4-RD with lung adenocarcinoma identified pathologically in May 2018, the clinical manifestation, pathological characteristics, diagnosis and treatment of IgG4-RD, and the relationship between IgG4-RD and malignant tumors were studied. Related Articles | Metrics

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Correlation between serum CA 19-9 levels and ovarian mature cystic teratoma: a Meta-analytic review ZHANG Chao, FU Jin-rui, WANG Gao-sheng 2019, 47 (2):  192-202.  doi: 10.11958/20181281 Abstract ( 647 )   PDF (1406KB) ( 3730 )    Objective To study the correlation between CA 19-9 levels and ovarian mature cystic teratoma systematically. Methods Data of the correlation between CA 19-9 and mature cystic teratoma were searched and collected from the database of Pubmed, CNKI, CBMdisc, and Wanfang. Two evaluators independently evaluated the quality of the literature and extracted the data. The literature will be grouped according to mature teratoma and immature teratoma, unilateral or bilateral lesions, with or without torsion, with or without neural tissue and thyroid tissue. Review Manager 5.3 was used for Meta-analysis. Results A total of 53 articles were included. The results were as follows: the concentration of CA 19-9 was lower in mature teratoma group (n=1 398) than that in immature teratoma group (n=145, P＜0.001). The concentration of CA 19-9 was higher in bilateral tumor group (n=614) than that in unilateral tumor group (n=3 126, P＜ 0.001). The concentration of CA 19-9 was higher in patients with tumor torsion group (n=18) than that in non-tumor torsion group (n=374, P＜0.001). There was no significant difference in the positive rate of CA 19-9 between the tumor group with nerve tissue (n=364) and the tumor group without nerve tissue (n=1 305, P=0.300). The positive rate of CA 19-9 was higher in the tumor tissue with thyroid tissue group (n=35) than that in tumor tissue without thyroid tissue group (n=1 135, P= 0.030). The positive rate of CA 19-9 decreased with the increase of age (r=- 0.818, P＜0.001) and increased with the increase of tumor size (r=0.927, P＜0.001). Conclusion There is a significant correlation between CA 19-9 concentration and MCT. There is higher concentration of CA 19-9 in the younger age group, which may indicate that the tumor is large, with the possibility of malignant, bilateral lesions, complications like tumor torsion, and the possibility of mixed thyroid tissue. Related Articles | Metrics

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Prospect of mesenchymal stem cells combined with graphene scaffold materials on knee osteoarthritis WANG Xiao-dong, LIU Ai-feng, JIN Hong-bin, LI Rong 2019, 47 (2):  203-207.  doi: 10.11958/20181370 Abstract ( 825 )   PDF (373KB) ( 4794 )    Knee osteoarthritis is one of diseases that afflict the middle-aged and old people. Articular cartilage injury is the core of its pathological process. The pathological researches around cartilage damage have become more and more in- deepth, and the existing treatment cannot fundamentally reverse this process. However, with the development of cartilage tissue engineering technology in recent years, especially the use of mesenchymal stem cells has provided a new way to solve this difficulty. At the same time, the application of graphene materials in the medical field has gradually become popular. The use of graphene material properties as a scaffold structure combined with mesenchymal stem cells to seek a new method for cartilage damage repair, this paper will summarize the mechanism of knee cartilage injury, the repair of cartilage by mesenchymal stem cells and the application of graphene scaffold materials, in order to develop new treatment ideas. Related Articles | Metrics

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Research progress of intestinal flora in colitis associated cancer KONG Xian-bin, WANG Fang-yuan, PENG Ying-ying, GUO Yu-zhu, MENG Jing-yan 2019, 47 (2):  207-212.  doi: 10.11958/20181639 Abstract ( 672 )   PDF (401KB) ( 3743 )   Colitis associated cancer (CAC) is a worldwide medical problem. Tumor microenvironment is the hot spot of current research. Intestinal flora imbalance is closely related to the incidence of CAC and aggravates colitis-induced CAC. With the development of microecology, intestinal flora has received much attention in CAC research. This article outlines the relationship between intestinal flora and diet, inflammatory mechanisms and intestinal mucosa during CAC development, and the related research in diagnosis and treatment. It is mainly discussed that the intestinal flora may become a new target of CAC mechanism and its related application prospects. Related Articles | Metrics

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Expression of ERG gene in tumor and its clinical research progress WANG Wen-jun, YU Yu, CUI Jiu-wei 2019, 47 (2):  213-220.  doi: 10.11958/20181058 Abstract ( 2149 )   PDF (539KB) ( 8978 )   ETS-related gene (ERG) belongs to the E26 transformation-specific (EST) transcription factor family and plays an important role in the development and production of blood vessels, hematopoietic system and cartilage development. In recent years, more and more studies have found that ERG gene overexpression occurs in some tumors, thereby promoting tumor cell proliferation and tumor angiogenesis, and involving in the occurrence and development of tumors, such as prostate cancer (PCa), Ewing's sarcoma (EWS), leukemia, cartilage tumors. However, the specific mechanism of the action of ERG in these tumors is not yet fully clear. At present, the application of ERG in the diagnosis and prognosis of tumors has become a research hotspot. Some drugs that target ERG and its downstream signaling pathways and target genes are in clinical trials. This article reviews the expression, mechanism of action and clinical application of ERG gene in tumors. Related Articles | Metrics

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Research progress of severe fever with thrombocytopenia syndrome and its etiology WANG Jing-jie, LIU Zhi-xin, WEI xiu-li , YANG Jing, LI Jian, TAN Hua-bing, LIU Long 2019, 47 (2):  220-224.  doi: 10.11958/20181245 Abstract ( 770 )   PDF (404KB) ( 3644 )   Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly discovered RNA virus belonging to the family Bunyaviridae, which has been identified as the causative agent of severe fever with thrombocytopenia syndrome (SFTS). SFTSV mainly appears in rural and hilly areas in central and eastern provinces of China, and has caused serious public health problems. SFTS presents mainly have symptoms including fever, vomiting, peripheral blood thrombocytopenia, liver and kidney function damage. Some patients died due to multiple organ dysfunction, and the mortality rate has reached to 10%-15%. This review focused on the research progress in the etiology, infection process, pathogenesis, epidemiology, clinical diagnosis and detection methods of SFTSV, and put forward the existing problems and preventive measures to provide a reference for the diagnosis, prevention and control of this disease. Related Articles | Metrics