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15 December 2021, Volume 49 Issue 12 Previous Issue    Next Issue

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3-O-C12-HSL hampers the maturation of Mo-DCs by inhibiting the expression of lncRNA CD48-AS and CD48 LUO Yan-fen, ZHUANG Qi-zhen, ZHANG Xuan, CHEN Cha, LIU Yang, YAN Xing-xing, XIAO Qian, LI You-qiang 2021, 49 (12):  1233-1239.  doi: 10.11958/20210909 Abstract ( 522 )   PDF (1437KB) ( 2888 )   Objective To screen and clarify the mechanism of long non-coding RNA CD48-AS (lncRNA CD48-AS) and the antisense complementary molecule CD48 in the process of N-3-oxododecanoyl-L-homoserine lactone (3-O-C12- HSL) hampering the maturation of human monocyte-induced dendritic cells (Mo-DCs). Methods Mo-DCs were extracted from the peripheral blood of healthy individuals, and the immature Mo-DCs were divided into three groups for lncRNA chip analysis: the negative control group (0.1% DMSO), the lipopolysaccharide (LPS) positive control group (100 μg/L LPS) and the treatment group (100 μg/L LPS+40 μmol/L 3-O-C12-HSL). The natural antisense lncRNA expressed more than 5 times in the lncRNA expression profile were screened for Cluster analysis. The natural antisense lncRNA CD48-AS was screened. Immature Mo-DCs were divided into the negative control group, the LPS positive control group, and the 3-O-C12-HSL treatment group (5, 10, 25 μmol/L 3-O-C12-HSL). The expression of lncRNA CD48-AS and antisense molecule CD48 were measured using quantitative PCR in each group. The complementary regions of lncRNA CD48-AS and CD48 and the protein coding function of lncRNA CD48-AS were analyzed through bioinformatics. Whether lncRNA CD48-AS affected the expression of CD48 by forming a dimer with CD48 was verified through the ribonuclease protection experiment (RPA). Preliminary experiments were carried out on the localization of lncRNA CD48-AS in cells. Results The lncRNA expression profile of Mo-DCs showed specific changes after 3-O-C12-HSL treatment. 3-O-C12-HSL down-regulated the expression of lncRNA CD48-AS and its antisense target molecule CD48 induced by LPS. Bioinformatics analysis showed that lncRNA CD48-AS was a non-coding RNA without protein coding function. lncRNA CD48-AS may form RNA dimers with CD48 to reduce the degradation of CD48 by RNase and increase the expression of CD48. lncRNA CD48-AS was mainly located in the nucleus in Mo-DCs, with less expression in cytoplasm. Conclusion 3-O-C12-HSL can inhibit the maturation of Mo-DCs by down-regulating lncRNA CD48-AS and then affecting the expression of CD48. Related Articles | Metrics

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Effects of hypoxia on proliferation and the expression of HIF-1α, VEGF, MMP-9 and TIMP-1 in HTR-8/SVneo cells YUAN Shuo, LIU Xiang-yun, ZHANG Jia-qi, DENG Gao-pi 2021, 49 (12):  1240-1244.  doi: 10.11958/20211483 Abstract ( 576 )   PDF (592KB) ( 2855 )   Objective To investigate the effects of hypoxia on the proliferation of trophoblast cell line HTR-8/SVneo cells and the protein expression of hypoxia inducer (HIF) -1α, vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP) -9 and tissue inhibitor of matrix metalloproteinase 1 (TIMP-1). Methods HTR-8/SVneo cells were treated with 0, 50, 100, 200, 400, 800, 1 000, 1 200 μmol/L CoCl2 to establish the chemical hypoxia model. The proliferation of HTR-8/SVneo cells was detected by CCK8 method after 24 h and 48 h culture at the above concentration. According to the results of CCK8 experiment, the hypoxic, medium and high concentration groups were determined. Western blot assay was used to detect changes of the protein expression levels of HIF-1α, VEGF, MMP-9 and TIMP-1 in HTR-8/ SVneo cells in the hypoxic, medium and high concentration groups. Results CCK8 experiment showed that hypoxia activated the proliferation of HTR-8/SVneo cells. After treatment with CoCl2 on HTR-8/SVneo cells for 48 hours, the OD value increased with the increase of concentration. Under the same CoCl 2 concentration, the OD value of 48 h was higher than that of 24 h. The 100, 200 and 400 μmol/ L CoCl2 were used as the low, medium and high concentration groups. After 48 hours, compared with the blank group, the expression levels of HIF-1α, MMP-9 protein in HTR-8/SVneo cells were upregulated in the low, medium and high concentration groups (P＜0.05). The ratio of MMP-9/TIMP-1 increased in a concentration dependent manner (P＜0.05). The protein expression of VEGF was upregulated in the hypoxia, medium and high concentration groups (P＜0.05). The expression level of TIMP-1 protein decreased in the low, medium and high CoCl2 groups, but it showed an increased trend with the increased CoCl2 concentrations. Conclusion Hypoxia can enhance the proliferation of HTR-8/SVneo cells, and the expression of VEGF and the ratio of MMP-9/TIMP-1 can be upregulated by HIF-1α. Related Articles | Metrics

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Resveratrol prevents inflammation and oxidative stress response in HGFs LI Li-hua, WANG Yu-jiao, LI Jun-xiong, LI Si-yu, TANG Wan-rong, QIU Ya 2021, 49 (12):  1245-1249.  doi: 10.11958/20210918 Abstract ( 563 )   PDF (696KB) ( 2865 )   Objective To elucidate the anti-inflammatory and anti-oxidative properties of resveratrol (RSV) on Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide- (LPS- ) induced human gingival fibroblasts (HGFs). Methods HGF cells were divided into experiment 1 and experiment 2. Cells in experiment 1 were divided into the control group, the LPS group, the RSV 20 μmol/L group, the RSV 40 μmol/L group, the RSV 80 μmol/L group and the LPS+RSV 20 μmol/L group. The cells in experiment 2 were divided into the control group, the LPS group, the LPS+RSV 40 μmol/L group, the LPS+RSV 40 μmol/L+E5564 group and the LPS+E5564 group. Cell viability was evaluated by cell-counting kit-8 assay. IL-1β, IL-6, IL-8, TNF-α, SOD, MDA, and GSH-Px levels were measured by ELISA. Protein expressions were measured by Western blot analysis. Results It was found that 20, 40 and 80 μmol/L RSV had no significant effects on the viability of HGF cells. In LPSinduced HGFs, 40 and 80 μmol/L RSV significantly reduced inflammation by the down-regulation of IL-1β, IL-6, IL-8, and TNF- α expression. And 40 and 80 μmol/L RSV also decreased MDA expression, accompanied by an increase of SOD production (P＜0.05). Meanwhile, 80 μmol/L RSV significantly increased GSH-Px levels (P＜0.05). Additionally, 20, 40 and 80 μmol/L RSV induced deactivation of TLR4/MyD88/NF- κB signaling pathway (P＜0.05). It was found that TLR4 inhibitor (E5564) could further strengthen RSV-reduced inflammation and OS injury by the down-regulation of IL-1β, IL-6, IL-8, and TNF- α production and up-regulation of GSH-Px in LPS-induced HGFs (P＜0.05). Conclusion Resveratrol attenuates the inflammation and OS injury of P. gingivalis LPS-treated HGFs by deactivating TLR4/MyD88/NF-κB signaling pathway. Related Articles | Metrics

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Research on the mechanism of DNMT1 involved in the growth and metastasis of cervical cancer by down-regulating the expression of SOX1 ZHI Yan-fang, ZHANG Ting, LIU Hui, ZENG Xian-xu, BAN Zhen-ying, ZHANG Wei 2021, 49 (12):  1250-1255.  doi: 10.11958/20211084 Abstract ( 526 )   PDF (851KB) ( 2876 )   Objective To investigate the effect of DNA methyltransferase 1 (DNMT1) on the growth and metastasis of cervical cancer (CC) by regulating the expression of sex determining region Y-box 1 (SOX1). Methods Bisulfite quantitative PCR was used to determine the SOX1 methylation level in CC tissues and cells, CC adjacent tissues and normal cervical cells. Western blot assay was used to detect the protein expression levels of DNMT1 and SOX1. HeLa229 and SW756 cells were divided into the DNMT1-NC group, the DNMT1-siRNA group and the blank group. Bisulfite quantitative PCR was used to determine the SOX1 methylation ratio. Western blot assay was used to detect the DNMT1, SOX1, c-Myc, Cyclin D1 and β-catenin protein expression. The cell cloning and nude mouse tumor formation experiments were used to measure cell proliferation, and Transwell experiment was used to measure cell migration and invasion. Results The expression level of DNMT1 and the methylation ratio of SOX1 were higher in CC tissues than those in adjacent tissues, while the expression of SOX1 protein was significantly lower (P＜0.05). The expression level of DNMT1 and the methylation ratio of SOX1 in cervical cancer HeLa229, ME-180, SiHa, SW756 cells were significantly higher than those in cervical epithelial Ect1/E6E7 cells, while the expression of SOX1 protein was significantly lower (P＜0.05). Compared with the blank group and the DNMT1-NC group, the numbers of in vitro clone formation, migration and invasion of HeLa229 and SW756 cells decreased in the DNMT1-siRNA group, and the tumor mass was reduced in nude mice. The protein expression and SOX1 methylation ratio of DNMT1, c-Myc, Cyclin D1, and nuclear/plasma β -catenin decreased, SOX1 protein expression increased (P＜0.05). Conclusion DNMT1 is highly expressed in CC tissues and cells, and it may promote the growth and metastasis of CC by maintaining SOX1 promoter hypermethylation and inhibiting the protein expression. Related Articles | Metrics

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The effect of COL6A5 downregulated by m6A modification on lung adenocarcinoma cell invasion WANG Xin, HUANG Jia-xing, PAN Hui-lin, FENG Zheng-fu, TANG Rui-ming, QIU Hui-si 2021, 49 (12):  1255-1260.  doi: 10.11958/20202783 Abstract ( 602 )   PDF (1246KB) ( 3030 )   Objective To elucidate the role and expression pattern of COL6A5 in lung adenocarcinoma (LUAD). Methods The expression pattern of COL6A5 in LUAD was detected through Oncomine database. The relationship between COL6A5 and the prognosis of LUAD patients was analyzed by GEPIA database. The qPCR assay was used to detect the expression of RNA demethylase ALKBH5 in lung adenocarcinoma tissue. The m6A sites on COL6A5 was confirmed by bioinformatics analysis and MeRIP-qPCR experiment. The inhibitory effect of ALKBH5 on COL6A5 was detected by qPCR and Western blot assy. Bioinformatics analysis and Transwell assay were performed to determine the role of COL6A5 in LUAD. Results Oncomine analysis of the online database showed that the expression of COL6A5 was significantly reduced in LUAD. GEPIA database analysis showed that the level of COL6A5 mRNA expression was significantly lower in LUAD tissue than that of normal tissue (P＜0.05). qPCR result showed that COL6A5 mRNA expression was lower in LUAD tissues than that of adjacent normal tissues in 27 LUAD patients. Compared to normal pulmonary epithelial cells, COL6A5 mRNA was significantly inhibited in LUAD cells (P＜0.05). Furthermore, bioinformatics analysis and MeRIP-qPCR assay confirmed that there were m6A sites on COL6A5 mRNA. The expression level of ALKBH5 mRNA was higher in LUAD than that of adjacent normal tissues, and it was negatively correlated with COL6A5 mRNA expression (r=-0.599, P＜0.01). Knocking down ALKBH5 significantly enhanced COL6A5 expression in H1299 cells. After the analysis of co-expressed genes with COL6A5, it was found that COL6A5 may be involved in the regulation of life activities including epithelial transition, hemostasis, and cell surface interaction in LUAD cells. Results of Transwell assay and Western blot assay confirmed that the over-expression of COL6A5 could inhibit the invasion of H1299 cells. Conclusion The down regulated COL6A5 by ALKBH5 can inhibit the invasion metastasis of LUAD cells . Related Articles | Metrics

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Study on the changes of temporal frequency tuning curve of pattern visual evoked potentials in monocular-deprived amblyopic mice MA Bo-wen, LYU Wen-chao, WANG Jue, ZHANG Wei, SHI Xue-feng 2021, 49 (12):  1261-1264.  doi: 10.11958/20211190 Abstract ( 452 )   PDF (521KB) ( 3039 )   Objective To explore the changes of temporal frequency tuning of pattern visual evoked potentials in monocular-deprived amblyopic mice. Methods Eighteen postnatal 26 days C57 BL/6J mice were randomly divided into the normal control (Ctrl) group and the monocular deprivation (MD) group. The right eye of mice in the MD group was sutured with mattress for 5 days of form deprivation to establish the amblyopic model. The Ctrl group was fed in the same environment until postnatal 31 days. The electrodes were implanted on the dura above the visual cortex. The right eyes of the mice were subjected to pattern visual stimulation at different termporal frequencies (2.50, 1.25, 1.00, 0.75, 0.5 and 0.25 Hz) under anesthesia on postnatal 32 days, and pattern visual evoked potentials (PVEP) were recorded. The difference of P100 wave amplitudes of PVEP recorded under visual stimuli of different temporal frequencies was compared between the two groups. Results There were no significant differences in the amplitudes of PVEP P100 waveforms evoked by stimuli of 6 temporal frequencies between mice of the Ctrl group (F= 2.214, P＞0.05), while there were significant differences in the amplitudes of PVEP P100 waveforms evoked by stimuli of 6 temporal frequencies between mice of the MD group (F=6.588, P＜0.01), and its temporal frequency tuning curve showed low-passing feature. Under high temporal frequencies (2.50 and 1.25 Hz), the P100 wave amplitudes significantly decreased in the MD group compared with the Ctrl group (t= 2.362 and 2.425，P＜0.05). Under middle to low temporal frequencies (1.00, 0.75, 0.50 and 0.25 Hz), there was no significant difference in the P100 wave amplitudes in the MD group compared with the Ctrl group (all P＞0.05). Conclusion The PVEP responses to visual stimuli of high temporal frequencies are decreased in MD mice, but the responses to low temporal frequencies are not significantly influenced Related Articles | Metrics

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Effects and mechanism of APETx2 and fecal bacteria transplantation on the visceral sensitivity of rats with irritable bowel syndrome induced by neonatal maternal separation LI Huan, YAN Bo, WANG Jin-kun, YUAN Li-ping 2021, 49 (12):  1265-1270.  doi: 10.11958/20211135 Abstract ( 512 )   PDF (726KB) ( 2899 )   Objective To investigate the effect of fecal bacterial transplantation on visceral sensitivity of rats with irritable bowel syndrome (IBS) induced by neonatal maternal separation (NMS), and the role of acid-sensitive ion channel 3 (ASIC3) inhibitor APETx2 on visceral sensitivity in the development of IBS. Methods Ten pregnant SD rats were taken, and the IBS model was constructed by NMS after the birth. The feces of the successful model rats were collected to make fecal microbiota solution. Eighteen healthy male SD rats were randomly divided into the normal control group (Con group), the neonatal maternal separation group (NMS group) and the NMS+APETx2 treatment group, 6 rats for each group. The NMS group and NMS+APETx2 group were given ABX-water continuously for 5 days to establish the pseudo-aseptic model rats, then NMS rats were given intragastric administration of fecal microbiota solution (1.6 mL/kg). The Con group was given the equal volume of normal saline consecutively for 5 days, once a day. After the gavage, the NMS+APETx2 group was given 100 μg/kg APETx2 continuous intraperitoneal injection for 7 days, once a day. The ink movement test was used to determine the intestinal transit rate, and the visceral sensitivity was evaluated after colorectal balloon distention (CRD) stimulation. Immunohistochemistry was performed to detect the localization of ASIC3 and c-kit, and the changes of morphology and number of Cajal cells were observed. Results Compared with the Con group, the NMS group had slower intestinal transit rate, increased visceral sensitivity with the higher abdominal withdrawal reflex (AWR) scores, promoted expression of ASIC3 and c-kit in colon tissue, and morphological changes of ICC cells. Compared with the NMS group, there were significantly accelerated intestinal transit rate, decreased sensitivity of the intestine with the lower AWR scores, suppressed ASIC3 and c kit expression in colon tissue, and the morphology tended to be normal and the number of ICC was reduced in the NMS+ APETx2 group. Conclusion Fecal bacteria transplantation from IBS can induce visceral hypersensitivity and reduce intestinal transmission. APETx2 can alleviate visceral sensitivity and intestinal transmission, which may be related to the inhibition of ASIC3 expression and down-regulation of the c-kit signal associated Cajal cells. Related Articles | Metrics

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Study on the feasibility of renal artery ablation with high-frequency pulsed electric field ZHANG Jun-min, LU Cheng-zhi, XUE Zhi-xiao, SU Si-ying, LIU Shang-zhong 2021, 49 (12):  1270-1275.  doi: 10.11958/20211943 Abstract ( 528 )   PDF (1188KB) ( 2905 )   Objective To explore the feasibility and safety of high-frequency pulse electric field ablation in renal artery denervation. Methods Human neuroblastoma cell SH-SY5Y and rat thoracic artery smooth muscle cell A7r5 cells were ablated under the voltage of 500 V and 700 V, the number of pulses 500 and pulse mode: single pulse, long pulse, bidirectional pulse 5-5-5-5, bidirectional pulse 5-3-3-5 and bidirectional pulse 7-3-3-7. The cell survival rate was detected by MTT method. Electrodes were implanted by percutaneous femoral artery puncture, and renal artery sympathetic nervous system was ablated in four Bama miniature pigs. Surgical tissue melting sections were removed and observed under the microscope to verify the ablation effect. Results Under the microscope, A7r5 cells and SH-SY5Y cells were nearly completely apoptotic at 500 V and 700 V, respectively. When the voltage was 500 V, the survival rate was higher in SH SY5Y cells than that of A7r5 cells under the same pulse (P＜0.01). There was no significant difference in the survival rate of A7r5 cells between single pulse and long pulse, but it was lower than that of bidirectional pulse 5-5-5-5 (P＜0.05). The survival rate increased gradually in bidirectional pulse 5-5-5, 5-3-3-5 and 7-3-3-7 (P＜0.05). When the voltage was 700 V, the cell survival rate was higher in A7r5 cells after two-way pulse 5-3-3-5 ablation than that of two-way pulse 5-5-5-5 (P＜0.05). There was no significant difference in cell survival rate between other pulse modes. During the ablation of porcine renal artery, slight convulsions occurred under the bidirectional pulse 5-5-5-5 mode. The ablation process of bidirectional pulse 5-3-3-5 and 7-3-3-7 was stable. After ablation, the staining of renal artery showed focal rupture of adventitia and media, wall fibrosis, local endothelial damage and thrombosis. Masson staining showed arterial wall fibrosis, and silver nitrate staining showed light brown nerve tissue and nerve cell death. Conclusion High frequency pulsed electric field has good ablation effect on smooth muscle cells A7r5 and nerve cells SH-SY5Y. High frequency pulsed electric field can ablate porcine renal artery sympathetic nerve. Bidirectional asymmetric pulse 5-3-3-5 can effectively avoid muscle contraction, which is the optimal parameter array. Related Articles | Metrics

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The effect of curcumin on CYP3A in rats with alcoholic liver injury and its mechanism CHEN Jie, LIU Bin, YUAN Qiao-yu 2021, 49 (12):  1276-1281.  doi: 10.11958/20210852 Abstract ( 437 )   PDF (557KB) ( 2840 )   Objective To investigate the effects of curcumin on cytochrome P450 3A (CYP3A) in rats with alcoholic liver injury (ALD) and its mechanism. Methods Sixty ALD rats with successful modeling were randomly divided into the model group, the curcumin low, medium and high dose groups (gavage 40, 80 and 160 mg/kg curcumin respectively) and the positive control group (200 mg/kg ademetionine intraperitoneally), with 12 rats in each group. The control group (n=12) was fed normally and given intragastric administration of equal volume of normal saline, for 6 consecutive weeks. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were detected by automatic biochemical analyzer. The morphological changes of liver were detected by hematoxylin-eosin (HE) staining. The mRNA levels of pregnane X receptor (PXR), constitutive androstane receptor (CAR) and CYP3A25 were detected by quantitative real-time PCR (qPCR). The protein levels of PXR and CAR were detected by Western blot assay. Rat primary hepatocytes were used to culture with 0, 100, 200, 300, 400 and 500 mmol/L alcohol respectively, and the alcohol concentration was taken when the cell proliferation inhibition rate was about 50% for the next experiment. 0, 0.5, 1.0, 2.0, 4.0, 8.0 and 16.0 μmol/L curcumin cultured cells respectively, and the most suitable curcumin concentration was used for the next study. The experiments were divided into the control group, the alcohol group, the curcumin group, the curcumin+siRNA-NC group and the curcumin+ siRNA-CYP3A25 group. The cell proliferation was detected by CCK-8. The level of CYP3A25 mRNA in cells was detected  by qPCR. The levels of PXR and CAR protein in cells were detected by Western blot assay. Results In the rat experiment: compared with the control group, the serum levels of ALT, AST and ALP increased in the model group (P＜0.05). Compared with the model group, the serum levels of ALT and ALP decreased in the curcumin low dose group, and the levels of PXR, CAR mRNA and protein, CYP3A25 mRNA in liver tissue increased (P＜0.05). The serum levels of ALT, AST and ALP decreased in the curcumin medium and high dose groups, while the levels of PXR, CAR mRNA and protein, CYP3A25 mRNA in liver tissue increased (P＜0.05). The indicators gradually recovered with the increase of dosage. In the cell experiments: compared with the control group, the cell proliferation inhibition rate increased in the alcohol group (P＜0.05), and the levels of PXR and CAR protein in cells decreased (P＜0.05). Compared with the alcohol group, the proliferation inhibition rate decreased in the curcumin group (P＜0.05), the levels of CYP3A25 mRNA, PXR and CAR protein in cells increased (P＜0.05). Compared with the curcumin group, the proliferation inhibition rate increased in the curcumin+siRNA CYP3A25 group (P＜0.05), and the levels of CYP3A25 mRNA, PXR and CAR protein in cells decreased (P＜0.05). Conclusion Curcumin can increase the level of CYP3A25 to promote drug metabolism and alleviate ALD. This process may be related to the increase expression of PXR and CAR. Related Articles | Metrics

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The relationship between fibrinogen to albumin ratio and early neurological deterioration after rt-PA intravenous thrombolysis in patients with acute ischemic stroke GU Chun-qing, ZHANG Yun-ke, YANG Guang-hua, WU Ji-tao 2021, 49 (12):  1282-1286.  doi: 10.11958/20211514 Abstract ( 757 )   PDF (449KB) ( 2834 )   Objective To explore the relationship between fibrinogen to albumin ratio (FAR) and the early neurological deterioration (END) after intravenous thrombolysis with recombinant tissue-type plasminogen activator (rt-PA) in patients with acute ischemic stroke (IS). Methods A total of 156 patients with IS who received rt-PA intravenous thrombolysis were included. According to the occurrence of END, patients were divided into the END group (n=36) and the non-END group (n= 120). The baseline data were collected. Pearson test was used to analyze the correlation between the FAR of IS patients and the National Institutes of Health Stroke Scale (NIHSS) score before thrombolysis. The receiver operating characteristic (ROC) curve was used to analyze the value of relevant indicators to predict END after rt-PA intravenous thrombolysis in IS patients. Multivariate Logistic regression analysis was used to analyze the risk factors of END after rt-PA intravenous thrombolysis in IS patients. Results The incidence of END after rt-PA intravenous thrombolysis was 23.08% in patients with acute IS. The NIHSS score, blood sugar, white blood cell count, serum fibrinogen (FIB) level and FAR were significantly higher before thrombolysis in the END group than those of the non-END group, and the serum albumin (ALB) level was significantly lower in the END group than that of non-END group (P＜0.05). The FAR of IS patients was positively correlated with NIHSS score before thrombolysis (P＜0.05). The area under curve of FAR predicting END after rt-PA intravenous thrombolysis in IS patients was 0.806 (0.710-0.902), which was significantly higher than that of ALB［0.609(0.494-0.724)］and FIB［0.639 (0.524-0.754)］; but it was lower than the AUC of NIHSS score［0.963(0.931-0.994)］before thrombolysis (P＜0.05). The increased FAR and NIHSS score before thrombolysis were independent risk factors for END after rt-PA intravenous thrombolysis in IS patients (P＜0.05). Conclusion The increase of FAR is related to END after rt-PA intravenous thrombolysis, which can be used as a basis for predicting the occurrence of END after rt-PA intravenous thrombolysis in IS patients. Related Articles | Metrics

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Comparison of the effects between centrifugal pump and roller pump on platelets during Stanford type A aortic dissection cardiopulmonary bypass JI Zhen-hua, WU Ting, JIANG Nan 2021, 49 (12):  1287-1291.  doi: 10.11958/20211132 Abstract ( 400 )   PDF (442KB) ( 2908 )   Objective To compare the effect of centrifugal pump and roller pump on platelet in Stanford A-type aortic dissection under cardiopulmonary bypass (CPB). Methods A total of 40 patients who planned to undergo a surgery were enrolled, and they were divided into the centrifugal pump group (n=20) and the roller pump group (n=20) according to the random number table. Data of CPB time, aortic cross-clamp time, circulatory arrest time, blood consumption, coagulation function, liver and kidney function, blood routine, postoperative chest drainage, mechanical ventilation time, ICU (intensive care unit) length of stay, length of hospital stay, and complications were recorded in the two groups. Platelet α -granule membrane protein 140 (GMP-140) and platelet factor 4 (PF4) concentration and platelet count were detected after induction of anesthesia (T1), 0.5 h after fish sperm neutralization (T2), 4 h in ICU (T3), 24 h in ICU (T4), and 48 h in ICU (T5). Results There were no significant differences in baseline data and intraoperative indicators (CPB time, aortic cross-clamp time, circulatory arrest time, blood consumption, bleeding volume) between the two groups (P＞0.05). At T2-T4, compared with the roller pump group, platelet count was increased, and the concentration of PF4 and GMP140 decreased in the centrifugal pump. At 4 h and 24 h, the chest drainage and blood consumption were significantly less in the centrifugal pump group than those in the roller pump group (P＜0.05). There were no significant differences in mechanical ventilation time, ICU stay time, length of hospital stay, 48 h chest drainage and complications between the two groups (P＞0.05). Conclusion In the operation of Stanford type A aortic dissection, the centrifugal pump can reduce platelet activation and consumption, and reduce postoperative bleeding and blood consumption. Related Articles | Metrics

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The value of microRNA-1 in the diagnosis and prediction of glucocorticoid therapy sensitivity of acute onset of bronchial asthma CHEN Di, DAI Biao, CHEN Ru-hua 2021, 49 (12):  1291-1296.  doi: 10.11958/20210571 Abstract ( 456 )   PDF (460KB) ( 2803 )   Objective To investigate the relationship between peripheral blood microRNA-1(miR-1) and acute attack of bronchial asthma, and its value in predicting the sensitivity of hormone therapy in patients with acute attack of bronchial asthma. Methods A total of 72 patients with acute exacerbation of bronchial asthma were used as the observation group, including 16 patients without inhaled glucocorticoid (ICS), 45 patients with steroid sensitive type (SS), and 11 patients with steroid resistant type (SR). In addition, 30 outpatients with health check-ups in the same period were included as the control group. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the serum expression level of miR-1. The relationship between miR-1, interleukin (IL)-5, IL-6, IL-8, tumor necrosis factor α (TNF-α), interferon-γ (IFN-γ), immunoglobulin E (IgE), and fractional exhaled nitric oxide (FeNO) was analyzed by using Spearman rank correlation analysis. The receiver operating characteristic (ROC) curve was used to evaluate the predictive value of miR-1 in diagnosing the acute onset of bronchial asthma and the sensitivity of glucocorticoid therapy. Results The expression levels of miR-1 were significantly lower in the SS group and the SR group than those of the ICS-free group and the control group (P＜0.05). Spearman rank correlation analysis showed that miR-1 was positively correlated with IFN-γ, and negatively correlated with IL-5, IL-6, IL-8, TNF-α, IgE, and FeNO (all P＜0.01). ROC curve analysis showed that in the diagnosis of acute attack of bronchial asthma, the area under the curve (AUC) of miR-1 was higher among all test markers (AUC=0.794, 95%CI: 0.706- 0.882, P＜0.001). The results of joint diagnosis showed that the combination of miR-1 and IFN-γ had higher diagnostic efficiency (AUC=0.834, 95%CI: 0.757-0.912, P＜0.001). When predicting glucocorticoid sensitivity in asthmatic patients, the predictive value of miR-1 was fair (AUC=0.797, 95%CI: 0.668-0.926, P=0.002). The results of combined diagnosis showed that miR-1 combined with FeNO had higher diagnostic value (AUC=0.976, 95%CI: 0.928-1.000, P＜0.001). Conclusion The serum expression of microRNA-1 in patients with bronchial asthma is reduced, and it is expected to be a potential biomarker for the diagnosis of acute asthma. The level of miRNA-1 in peripheral blood of glucocorticoid resistant asthma patients is further reduced, suggesting that it can be one of the detection indicators used as a predictor of the sensitivity of glucocorticoid therapy in asthma patients. Related Articles | Metrics

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Long-term prognostic analysis of simultaneous integration and intensity modulation combined with selective lymph node radiation radical therapy for esophageal squamous cell carcinoma LI Yun-hua, YAN Xue-jun, TAN Ya-li, HU Ya, XIAO Mao-liang 2021, 49 (12):  1297-1302.  doi: 10.11958/20211069 Abstract ( 473 )   PDF (406KB) ( 2777 )   Objective To investigate the long-term prognosis of simultaneous integrated intensity modulation (SIBIMRT) combined with selective lymph node irradiation (ENI) with or without chemotherapy for radical treatment of esophageal squamous cell carcinoma. Methods Data of 130 patients with esophageal squamous cell carcinoma who received radical radiotherapy were analyzed retrospectively, and the long-term survival, prognostic factors, treatment failure mode and adverse reactions were analyzed. Results The 1- , 3- and 5-year overall survival (OS) and progression-free survival (PFS) rates were 81.5%, 52.7%, 39.8% and 70.7%, 40.6%, 35.8%, respectively. The median OS stage and PFS stage were 36.37 months and 23.89 months, respectively. Multivariate Cox analysis showed that cT3, cN1, no chemotherapy and GTV-V＞37.0 cm3 were independent risk factors for OS, and cT3 was independent risk factors for PFS (P＜0.05). In all patients, 39 patients (30.0%) had local recurrence, 20 patients (15.4%) had distant metastasis, and 4 patients had both. Among the 35 patients with local regional recurrence, there were 32 cases of local esophageal lesion recurrence, 1 case of regional lymph node recurrence, and 2 cases of local esophageal combined regional lymph node recurrence. All patients had no grade≥4 related adverse reactions during and after treatment. There was no significant difference in the incidence of grade≥2 adverse reactions between patients with combined chemotherapy and patients without combined chemotherapy. Conclusion SIB-IMRT combined with ENI is safe and effective in the treatment of patients with esophageal squamous cell carcinoma. It is recommended to combine chemotherapy for patients with better general conditions. Related Articles | Metrics

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Efficacy analysis of F4.8 visual nephroscope under ultrasound-guided spermatic cord block in the treatment of benign epididymal tumors 2021, 49 (12):  1303-1307.  doi: 10.11958/20211508 Abstract ( 472 )   PDF (541KB) ( 2817 )   Objective To evaluate the safety and efficacy of 4.8Fr micro-percutaneous nephrolithotomy in the diagnosis and treatment of epididymal benign mass under ultrasound-guided spermatic cord block. Methods Eighty-two patients diagnosed with epididymal mass underwent scrotal surgery with the application of 4.8Fr micro-percutaneous nephrolithotomy from January 2018 to May 2021 in our hospital. They were randomly divided into conventional blind puncture group (group R, 41 cases) and ultrasound-guided puncture group (group U, 41 cases). The surgical efficacy, scrotum pain score, complications and recurrence of lesions were recorded. Results All the 82 patients were successfully performed scrotal cystoscopy without severe complications occurred, such as scrotal hematoma, testicular rupture or epididymis injury. Sixty-four cases diagnosed with epididymal cyst accepted laser resection of the cyst. Eighteen cases of epididymal solid mass were diagnosed as incompletely torsion of the testicular or epididymosis accessory, and underwent holmium laser ablation. The U group had higher successful rate of spermatic cord block, lower rate of vascular complications and conversion of anesthesia mode, compared with R group, which had significantly statistical differences (P<0.05). However, there was no significant difference in intraoperative anesthetic drug addition between two groups (P>0.05). There were significantly statistical differences according to the pain score of 5 min and 2h, and hospitalization cost between two group (P<0.01), while the differences of pain score of 6h and 12h, and time of hospitalization were not significant (P> 0.05). The scrotal ultrasonography was performed after 1 and 3 month, and no abnormal structure was observed. Conclusion The technique of 4.8Fr micro-percutaneous nephrolithotomy under ultrasound-guided spermatic cord block could be an ideal inspection and treatment method for epididymal benign mass, and it is worth promoting. Related Articles | Metrics

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A clinical comparative study on the safety and feasibility of transumbilical single-port+1-port laparoscopy and traditional laparoscopic radical resection of colorectal cancer 2021, 49 (12):  1307-1310.  doi: 10.11958/20211357 Abstract ( 576 )   PDF (419KB) ( 2812 )   Objective To compare the safety and feasibility of transumbilical single-hole+1-hole laparoscopy and traditional laparoscopic radical resection of colorectal cancer. Methods Data of 42 patients with colorectal cancer admitted to our hospital from October 2018 to May 2021 were collected. Patients were divided into the study group and the control group according to different surgical methods, and 21 patients in each group. The two groups were treated with umbilical single-hole+ 1-hole laparoscopic radical mastectomy and traditional laparoscopic radical mastectomy. Data of operation time, intraoperative blood loss, postoperative exhaust time, postoperative hospital stay, number of lymph node dissection and postoperative complications were compared between the two groups. Results The postoperative hospitalization time was significantly shorter in the study group than that of the control group (P＜0.05). There were no significant differences in the operative time, intraoperative blood loss, postoperative exhaust time and number of lymph node dissection between the two groups (P＞ 0.05). There was no anastomotic fistula in the two groups after operation. There were one case of intestinal obstruction, one case of anastomotic bleeding and two cases of incision infection in the control group, and one case of anastomotic bleeding and one case of incision infection in the research group. There were no significant differences in the complications between the two groups (P＞0.05). Conclusion Transumbilical single-hole+1-hole laparoscopic radical resection of colorectal cancer is safe and feasible, and which is superior to traditional endoscopic surgery in postoperative recovery. Related Articles | Metrics

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The predictive value of microRNA-18a combined with uterine artery blood flow parameters for early-onset preeclampsia WANG Yong, LI Chuan-gang 2021, 49 (12):  1311-1315.  doi: 10.11958/20211196 Abstract ( 452 )   PDF (469KB) ( 2801 )   Objective To explore the predictive value of microRNA-18a (miR-18a) combined with uterine artery blood flow parameters [resistance index (RI), pulse index (PI) and systolic phase velocity/diastolic phase velocity (S/D)] for the early-onset preeclampsia (E-PE). Methods A total of 628 pregnant women undergoing pregnancy checkups were selected in this study and divided into the E-PE group (n=34) and the control group (n=594) according to whether they developed E-PE. The relative expression of mir-18a was detected by real-time quantitative PCR, and RI, PI and S/D parameters were detected by color Doppler ultrasound diagnostic instrument. The predictive value of serum mir-18a, RI, PI and S/D to E-PE were analyzed by subject operating characteristic curve (ROC). Results The relative expression of miR- 18a in serum was lower in the E-PE group than that of the control group, and RI, PI, S/D were higher than those of the control group (P＜0.05). The relative expression of serum miR-18a was negatively correlated with RI, PI, and S/D in E-PE patients (P＜0.05). ROC analysis showed that serum miR-18a, RI and PI can be used to predict the risk of E-PE, and areas under the curve were 0.780 (95%CI: 0.727-0.832), 0.729 (95%CI: 0.644-0.814) and 0.782 (95%CI: 0.695-0.870), respectively. However, S/D had lower predictive value for E-PE, and the product under the curve was 0.602 (95%CI: 0.496- 0.708). miR-18a combined with PI showed a higher predictive value for E-PE, with a sensitivity of 82.35%, a specificity of 83.84%, and a Youden index of 0.662. Conclusion The serum expression of in miR-18a is abnormally low in E-PE patients, and its expression level is negatively correlated with uterine artery blood flow parameters such as RI, PI, and S/D. miR-18a combined with PI has a high predictive value for E-PE. Related Articles | Metrics

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Study on the analgesic effects of different administration timing points on dressing change in patients with moderate second degree burn LIU Yu-shu, LIU Fei-fei, LI Jia-guang, LIU Wen-ming 2021, 49 (12):  1315-1318.  doi: 10.11958/20210702 Abstract ( 439 )   PDF (378KB) ( 2812 )   Objective To observe the effects of different administration timing of analgesics on the intensity and duration of pain caused by dressing changes on burn. Methods Forty male patients with lower limb burn were randomly divided into the experimental group (Pre group) and the control group (Post Group). Patients in Pre group were given flurbiprofen axetil 50 mg 30 minutes before dressing change, and patients in Post group were given flurbiprofen axetil with the same dose immediately after dressing change. The maximum heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP) and subjective pain score (numerical rating scale, NRS) were recorded before dressing change, and at the time points of 0.5, 1, 2, 4, 8, 14 and 24 hours after dressing change. The simultaneous assessment of NRS was also recorded. Results Compared with the Post group, HR, SBP and DBP were lower in the Pre group during dressing change and 1 hour after dressing change (P＜0.05). SBP was lower at 4,14 h and DBP at 8 h was also lower in the Post group (P＜ 0.05). Compared with the Post group, NRS was lower during dressing change and within 4 hours after dressing change in the Pre group (P＜0.05). Compared with the Post group, patients in the Pre group recovered to mild pain more quickly after dressing change (P＜0.05). Conclusion Thirty minutes administration of analgesics before dressing change can significantly reduce the intensity of dressing change pain and the duration of pain after dressing change. Related Articles | Metrics

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Determination of the cut-off value of Ki-67 and its effect on the early recurrence and metastasis in patients with HR+/HER2- breast cancer MA Qin, HOU Yu-jin, GAO Xu-tong, LIU Xin-lan 2021, 49 (12):  1319-1323.  doi: 10.11958/20210930 Abstract ( 1531 )   PDF (512KB) ( 2929 )   Abstract：Objective To determine the Cut-off value of Ki-67 expression in hormone receptor positive and human epidermal growth factor receptor 2 negative（HR+/HER2-） breast cancer, to explore the effect of different Ki-67 expression levels on early recurrence and metastasis of breast cancer. Methods We retrospectively analyzed 956 patients with HR+/HER2- breast cancer diagnosed by operation and pathology in our hospital from January 1, 2015 to December 31, 2019. The median was used to determine the Cut-off value of Ki-67 expression in HR+/HER2- breast cancer. Analysis the effect of Ki-67 expression on early recurrence and metastasis of HR+/HER2- breast cancer. Results The median expression of Ki-67 in 956 patients with HR+/HER2- breast cancer was 20%, There was no significant difference in the shape and position of Ki- 67 distribution curve among different years.20% was used as the cut off value for distinguishing the high and low expression of Ki-67 in our center. Compared with the international cut off value of Ki-67 (14%), the consistency of the two methods was better (Kappa = 0.846).956 patients were followed up for 4 to 71 months, a total of 56 patients had recurrence or metastasis. Univariate analysis showed that Ki-67 expression level, T stage, N stage, histological grade and vascular / nerve invasion were the influencing factors of early recurrence and metastasis in patients with breast cancer. Multivariate analysis showed that N stage (HR=2.130,95%CI:1.200~3.781) and Ki-67 ≥ 20% (HR=3.271,95%CI:1.441~7.421) were independent risk factors for disease-free survival (DFS) of HR+/HER2- breast cancer. The patients with Ki-67 ≥ 20% will be further divided into 3 subgroups: 20% ~ < 30%, 30% ~ < 50% and ≥ 50%. Compared with < 20% patients, with the increase of Ki-67 expression level, the risk ratio of early recurrence and metastasis increased (HR was 2.784, 4.409, 6.168), and 5-year DFS decreased (94.9%, 91.3%, 87.5%). Conclusion The Cut-off value of Ki-67 expression in HR+/HER2- breast cancer is 20% in our hospital. with the increase of Ki-67 expression, the risk ratio of early recurrence and metastasis with HR+/HER2- breast cancer was increased, DFS was decreased. Related Articles | Metrics

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Predictive value of serum pentraxin-3 combined with CHA2DS2-VASc score for cerebral infarction in patients with obstructive sleep apnea hypopnea syndrome and atrial fibrillation FENG Hui-qiong, DONG Feng, MENG Xiang-jun, NIU Wen-liang, XI Fu-qiang, WANG Wei 2021, 49 (12):  1324-1327.  doi: 10.11958/20211181 Abstract ( 456 )   PDF (436KB) ( 2935 )   Objective To investigate the prediction value of serum pentraxin 3 (PTX3) combined with CHA2DS2- VASc score for cerebral infarction in patients with obstructive sleep apnea hypopnea syndrome (OSAHS) and atrial fibrillation. Methods A total of 198 OSAHS patients with atrial fibrillation were selected. According to whether cerebral infarction occurred during the follow-up period, patients were divided into the cerebral infarction group (36 cases) and the non-cerebral infarction group (162 cases). The indexes were compared between the cerebral infarction group and the noncerebral infarction group. The correlation between serum PTX3 and other indexes in OSAHS patients with atrial fibrillation were analyzed. The risk factors of cerebral infarction in OSAHS patients with atrial fibrillation were analyzed. The predictive value of PTX3 combined with CHA2DS2-VASc score for cerebral infarction in patients with OSAHS complicated with atrial fibrillation was analyzed. Results The systolic blood pressure, low density lipoprotein cholesterol (LDL-C), left atrial diameter (LAD), apnea hypopnea index (AHI), CHA2DS2- VASc score and PTX3 were significantly higher in the cerebral infarction group than those in the non-cerebral infarction (P＜0.05). Serum PTX3 levels were positively correlated with LDL-C, LAD, AHI and CHA2DS2-VASc scores in patients with OSAHS and atrial fibrillation (P＜0.05). Multivariate Logistic regression analysis showed that the increased LAD, AHI, CHA2DS2-VASc score and PTX3 were risk factors for cerebral infarction in patients with OSAHS and atrial fibrillation (P＜0.05). The areas under the curve of PTX3 and CHA2DS2-VASc scores in predicting cerebral infarction in patients with OSAHS complicated with atrial fibrillation were 0.785 (0.695-0.875) and 0.794 (0.698-0.890), respectively. The combined analysis of PTX3 and CHA2DS2-VASc scores showed that the sensitivity and Youden index were significantly improved. Conclusion Serum PTX3 combined with CHA2DS2-VASc score has a higher predictive value for cerebral infarction in patients with OSAHS and atrial fibrillation. Related Articles | Metrics

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Establishment and evaluation of prognostic prediction model for ventriculo-peritoneal shunt in#br# post-traumatic hydrocephalus patients with severe disturbance of consciousness #br# BIAN Qin, YANG Peng, ZHANG Qiu-fang 2021, 49 (12):  1328-1332.  doi: 10.11958/20210135 Abstract ( 400 )   PDF (494KB) ( 2825 )   Objective To investigate the risk factors for the prognosis of ventriculoperitoneal shunt in patients with post-traumatic hydrocephalus complicated with severe disturbance of consciousness, and to construct a linear prediction model to guide the early identification of high-risk patients. Methods Patients diagnosed with post-traumatic hydrocephalus complicated with severe disturbance of consciousness in our hospital were selected as research objects. All patients were treated with ventriculoperitoneal shunt. Three months after treatment, patients were divided into the good prognosis group (GOS score ＞ 3 points) and the poor prognosis group (GOS score ≤3 points). Multivariate Logistic regression was used to screen risk factors, and a nomogram prediction model was built on this basis. The receiver operating characteristic (ROC) curve, area under curve (AUC) and Hosmer-Lemeshow test were used to evaluate the model. Results A total of 218 patients were included in the analysis, including 153 in the model group and 65 in the validation group. In the model group, 90 cases (58.8%) had a good prognosis, and 63 cases (41.2%) had a poor prognosis. Multivariate Logistic regression analysis showed that age ≥50 years (OR=1.356, 95%CI: 1.101-1.639), moderate hydrocephalus (OR=2.859, 95%CI: 2.325-3.212), Glasgow coma score 9-12 points at onset (OR=2.421, 95%CI: 2.056-2.857) and interval from onset to shunt≥3 months (OR=1.639, 95%CI: 1.325-2.124) were independent risk factors affecting prognosis (all P＜0.05). According to the above factors, the AUC of clinical prognosis were 0.896 (95%CI: 0.842-0.933) and 0.875 (95%CI: 0.825- 0.916) in the model group and the validation group respectively (P＜0.05). Hosmer-Lemeshow test showed that there was a good fitting degree for the line graph model (χ2=0.896 in the model group, χ2=0.567 in the verification group, both P＜ 0.05). After risk stratification based on the line graph model, the proportion of poor prognosis was significantly higher in high and medium risk patients than that of the low risk patients in the model group and the validation group. Conclusion The model constructed in this study has a high value in evaluating patients with post-traumatic hydrocephalus and severe disturbance of consciousness undergoing ventriculoperitoneal shunt, and which can guide the early clinical identification of high-risk patients. Related Articles | Metrics

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The report issuance template according to "Clinical Practice Guideline for Rapid on-site Evaluation of Diagnostic Interventional Pulmonology" FENG Jing 2021, 49 (12):  1333-1334.  doi: 10.11958/20211829 Abstract ( 440 )   PDF (331KB) ( 2852 )   In this instruction file, the definition and work content of rapid on-site evaluation (ROSE), the definition of ROSE cytomics and the qualification issues of ROSE report issuance are elaborated, and the report issuance template according to "Clinical Practice Guideline for Rapid on-site Evaluation of Diagnostic Interventional Pulmonology" is provided. Related Articles | Metrics

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The progress in the study of pathological changes and therapeutic strategies for repetitive mild traumatic brain injury CHEN Fang-lian, GE Xin-tong, WANG Dong, LEI Ping 2021, 49 (12):  1335-1339.  doi: 10.11958/20211348 Abstract ( 404 )   PDF (417KB) ( 2793 )   Mild traumatic brain injury (mTBI), known as brain concussion, accounts for most of traumatic brain injury (TBI) cases. Neurodegeneration could be observed in brains of some mTBI patients in the long term after injury. The underlying pathological mechanism has not been fully elucidated, which seriously affects the exploration and development of treatment strategies. On the basis of reviewing the procedure of pathological development in injured brain after moderate and severe TBI, this paper contrastively discussed the reported characteristics of pathological changes after repetitive mTBI. In addition, we also summarized the new progress in its therapeutic strategies, which will provide a basis for exploring longterm and comprehensive therapeutic strategies in the future. Related Articles | Metrics

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Research progress on the relationship between glial cells and synaptic plasticity ZHANG Ning, XIE Lu-shuang, LIU Qi, LYU Pei-ran 2021, 49 (12):  1340-1344.  doi: 10.11958/20211127 Abstract ( 771 )   PDF (406KB) ( 2791 )   Synaptic plasticity is closely related to the development and repair after injury in the nervous system. Glial cells maintain close communication with neurons through energy transportation and signal transduction, which is an important way to promote synaptic plasticity. Astrocytes are closely related to synapses in morphology and function, and are an important factor in regulating synaptic plasticity. Microglia carry out two-way information interaction with synapses, which is a key regulator of synaptic plasticity during development and adulthood. Oligodendrocytes participate in the formation of myelin sheaths, provide metabolic substrates for synaptic transmission, which is a potential force to promote synaptic plasticity. This paper reviews the research progress on the relationship between astrocytes, microglia, oligodendrocytes and synaptic plasticity, in order to provide the reference for further understanding the relationship between glial cells and synaptic plasticity. Related Articles | Metrics