天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (1): 25-29.doi: 10.11958/20160858

• 实验研究 • 上一篇    下一篇

人脐带间充质干细胞联合戈舍瑞林对 卵巢早衰的修复作用

贾晓晨 1， 鲁振宇 2， 孟文静 1， 史业辉 1   

  1. 1. 天津医科大学附属天津肿瘤医院
    2. 天津协和干细胞基因工程有限公司
    3. 天津医科大学附属肿瘤医院乳腺肿瘤内科
    4. 天津市肿瘤医院乳腺内科
  • 收稿日期:2016-08-19 修回日期:2016-11-22 出版日期:2017-01-15 发布日期:2017-01-15
  • 通讯作者: 鲁振宇 E-mail:luzhenyu_706@163.com

Effects of human umbilical cord mesenchymal stem cells combined with goserelin on repairing rat ovarian damage

JIA Xiao-chen1, LU Zhen-yu2, MENG Wen-jing1, SHI Ye-hui1   

  1. 1 Cancer Institute and Hospital, Tianjin Medical University, National Clinical Research Center of Cancer,
    Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin Clinical Research Center for Cancer,
    Tianjin 300060， China; 2 Tianjin Precision Cell Biotechnology Co.Ltd., Tianjin

  • Received:2016-08-19 Revised:2016-11-22 Published:2017-01-15 Online:2017-01-15

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摘要： 摘要： 目的 评价人脐带间充质干细胞（hUC-MSCs）联合戈舍瑞林对大鼠卵巢早衰模型的作用， 并观察 hUCMSCs 对化疗所致的损伤卵巢的趋化作用。方法 环磷酰胺首次负荷剂量 50 mg/kg 后， 以 8 mg/ （kg·d）的剂量连续 腹腔注射 14 d 建立大鼠卵巢早衰模型。随机数字表法将 120 只 SD 大鼠分为 A～E 组， 分别为正常组、 生理盐水对 照组、 戈舍瑞林组、 hUC-MSC 组和 hUC-MSC+戈舍瑞林组， 各 24 只。B～E 组通过腹腔注射环磷酰胺建立卵巢早衰 模型。分批处死大鼠， 测定血清雌二醇 （E2） 水平， 计数初级和成熟卵泡数目。通过 GFP 转染 MSCs， 使用共聚焦显微 镜检测通过尾静脉移植的 hUC-MSCs 在 SD 大鼠体内的分布和生长情况。动物活体成像系统观察 hUC-MSCs 在大 鼠体内分布情况。结果 第 46 天 E 组 E2水平与 A 组相当， E 组初始卵泡及成熟卵泡数与 A 组比较差异无统计学 意义（P＞0.05）。激光共聚焦显微镜下可见卵巢组织存在 GFP 阳性细胞的分布。结论 hUC-MSCs+戈舍瑞林组对 卵巢损伤的修复效果较好， 可使大鼠卵巢功能恢复到接近正常水平。

关键词:  脐带, 间质干细胞, 戈舍瑞林, 卵巢功能早衰, 抗肿瘤联合化疗方案

Abstract: Abstract: Objective To observe the repairing effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) and goserelin on chemotherapy-induced ovarian injury, and the distribution and growth of hUC-MSCs transplanted in rat chemotherapy-induced ovarian injury. Methods A total of 120 SD rats were randomized into group A-E: A normal group， B NS control group, C goserelin group, D hUC-MSCs group and E hUC-MSCs+goserelin group. The rat premature ovarian failure (POF) model was established by given a loading dose of cyclophosphamide (CTX, 50 mg/kg) followed by daily intraperitoneal injection of CTX (8 mg/kg) for consecutive 14-day. The hUC-MSCs were injected through caudal vein, and goserelin was given by subcutaneous injection 4 days before POF model established. The serum level of estrogen was detected and numbers of follicles were counted. After GFP was transfected by lentivirus, the distribution and growth of stem cells transplanted in rats were observed by animal in vivo imaging system. Results At day 46, the serum level of estrogen showed no significant difference between group A and group E (P > 0.05). There were no significant differences in the counted follicles between group A and group E (P > 0.05). After tail vein injection of the transfected cells, GFP positive cells were found in injury ovarian. Conclusion There is a repairing effect of hUC-MSCs and goserelin on ovarian injury.

Key words: umbilical cord, mesenchymal stem cells, Goserelin, ovarian failure, premature, antineoplastic combined chemotherapy protocols